Article Per Year (5 Year)
p-Index From 2020 - 2025
3.799
P-Index
This Author published in this journals
All Journal Jurnal Fitopatologi Indonesia Innofarm : Jurnal Inovasi Pertanian Agrovigor: Jurnal Agroekoteknologi Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Entomologi Indonesia Jurnal Pusat Inovasi Masyarakat
Fitrianingrum Kurniawati, Fitrianingrum
Departemen Proteksi Tanaman, Fakultas Pertanian, IPB University, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Medicine & Pharmacology Social Sciences

Published : 32 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 32 Documents
Search

 1   2   3   4 
Laporan Pertama di Sulawesi Selatan: Karakter Morfologi dan Molekuler Nematoda Puru Akar yang Berasosiasi dengan Akar Padi di Kabupaten Wajo, Sulawesi Selatan Hishar Mirsam; Fitrianingrum Kurniawati
Jurnal Perlindungan Tanaman Indonesia Vol 22, No 1 (2018)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.33108

Abstract

Root Knot Nematode (RKN) is one of the most important cosmopolite parasitic nematode species. Reports on RKN associated with rice root in Indonesia are still limited in West Java and Yogyakarta. This study aimed to identify the RKN associated with rice root in Sub-district of Bola, District of Wajo, South Sulawesi, based on morphological and molecular characters. Sampling was carried out by purposive method based on specific criteria of sample, i.e. root knot. Identification of root knot nematode (RKN) infestation in field was done by observing the primary and secondary symptoms. Morphological identification was carried out based on characters of juvenile 2 and the female perineal pattern. Molecular identification was based on amplification of r-DNA by polymerase chain reaction technique using primers rDNA2 and rDNA 1.58s. RKN were detected associated with the incidence of root knot in rice plant. RKN was identified as Meloidogyne graminicola based on morphological characters of juvenile 2 and the female perineal pattern. PCR using primer rDNA 2 / rDNA 1.58s successfully amplified a DNA band of RKN of ± 500 bp. Nucleotide sequence analysis showed that RKN isolated from Wajo was closely related to M. graminicola isolated from Nepal, China, India, Madagascar, and USA with homology of 98.1–100.00%. IntisariNematoda puru akar (NPA) merupakan salah satu jenis nematoda parasit penting yang bersifat kosmopolit. Laporan NPA yang berasosiasi dengan akar tanaman padi di Indonesia masih terbatas di Jawa Barat dan Yogyakarta. Penelitian ini bertujuan mengidentifikasi NPA yang berasosiasi dengan akar tanaman padi di Kabupaten Wajo, Sulawesi Selatan berdasarkan karakter morfologi dan molekuler. Pengambilan sampel dilakukan secara purposif dengan memilih sampel berdasarkan pada kriteria gejala spesifik penyakit puru akar. Identifikasi serangan dilakukan dengan mengamati gejala primer dan gejala sekunder. Identifikasi morfologi dilakukan dengan pengamatan karakter morfologi juvenil 2 dan pola perineal NPA betina.Identifikasi molekuler dilakukan dengan teknik polymerase chain reaction (PCR) untuk mengamplifikasi wilayah internal transcribed spacer (ITS) ribosomal DNA (rDNA) menggunakan pasangan primer rDNA2 dan rDNA1.58s. NPA ditemukan berasosiasi dengan akar tanaman padi yang memperlihatkan gejala puru akar. NPA diidentifikasi sebagai Meloidogyne graminicola berdasarkan karakter morfologi juvenil 2 dan pola perineal NPA betina. PCR menggunakan primer rDNA2/ rDNA1.58s berhasil mengamplifikasi pita DNA NPA dengan ukuran sekitar 500 bp. Analisis runutan nukleotida menunjukkan isolat NPA asal Wajo-Indonesia memiliki tingkat kekerabatan yang sangat dekat dengan isolat M. graminicola asal Nepal, Cina, India, Madagaskar, dan Amerika Serikat dengan nilai homologi berkisar 98,1–100,0%.
IDENTIFIKASI TOMATO INFECTIOUS CHLOROSIS VIRUS PENYEBAB PENYAKIT KLOROSIS PADA TANAMAN TOMAT DI CIPANAS JAWA BARAT MELALUI PERUNUTAN NUKLEOTIDA GEN PROTEIN SELUBUNG UTAMA Fitrianingrum Kurniawati; Gede Suastika; Giyanto .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 15 No. 1 (2015): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.34 KB) | DOI: 10.23960/j.hptt.11533-43

Abstract

Identification of tomato infectious chlorosis virus, the causal agent of chlorosis disease on tomato in Cipanas West Java by sequencing of main coat protein gene nucleotide. Tomato infectious chlorosis virus (TICV) causes chlorosis on tomato. Tomatoes infected by this virus shows interveinal yellowing, necrotic, bronzing, brittleness, and declining in productivity. This study aims to identify the causal agent of chlorotic disease on tomato by sequencing the coat protein gene. The methods involve collecting infected plants, total RNA extraction, cDNA synthesis, DNA amplification, visualization of the results of reverse transcription polymerase chain reaction (PCR), and phylogenetic analysis using BLAST, clustal w, Bioedit v 7.0.5.3, MEGA v 6:06. RT-PCR using spesific primers (CP-F TICV Bam and TICV R-Hind) amplified a DNA band of 792 bp, which has been successfully sequenced and identified as TICV. Nucleotide sequences homology analysis showed that TICV Indonesia_TWJ isolate Cipanas is the same strain as TICV from other countries (99.4 – 100%), such as Spain, Greece, USA, France, and Italy.
EKSPRESI GEN PROTEIN SELUBUNG TOMATO INFECTIOUS CHLOROSIS VIRUS PADA ESCHERICHIA COLI Fitrianingrum Kurniawati; Gede Suastika; Giyanto .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 15 No. 2 (2015): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.527 KB) | DOI: 10.23960/j.hptt.215114-121

Abstract

Expression of tomato infectious chlorosis virus coat protein gene on Escherichia coli. Tomato infectious chlorosis virus (TICV) is the causal agent of chlorotic disease of tomato. Detection of TICV can be carried out by RT-PCR and serological test. Titer of TICV in plant tissue is very low because TICV is limited to phloem. Serological detection of TICV requires antiserum which is not available in Indonesia. Producing antibody through cloning and coat protein gene (TICV CP gene) expression is a promising approach in producing antiserum. The objective of this study was to express TICV CP gene as antigen for antiserum production. TICV CP gene was amplified using RT-PCR from total RNA extracted from TICV infected leaves collected from Cipanas, Cianjur, West Java. The amplified CP gene was then sequenced and sub-cloned into pET 21b expression vector, transformed into Escherichia coli strain BL21 DE3(pLysS) and induced expression using IPTG 1 mM overnight at 37 °C. CP that contains 6xhistag was purified using NiNTAspin column and then confirmed by SDS-PAGE. The size of TICV CP gene was 750 bp and the gene was expressed on pET 21 b vector and SDS-PAGE showed a 29 kDa band.
Penggunaan Drone dalam Mengaplikasikan Pestisida di Daerah Sungai Besar, Malaysia Hana Khoirunisa; Fitrianingrum Kurniawati
Jurnal Pusat Inovasi Masyarakat (PIM) Vol. 1 No. 1 (2019): November 2019
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (346.723 KB)

Abstract

Agriculture is an important thing that must be considered because all organisms including humans need food to live. In the industrial era 4.0 technology in the field of agriculture began to develop, starting from before planting to harvesting. In Sungai Besar, Selangor, Malaysia, almost all agricultural activities are already using machines and technology-based. One of them is the presence of drones owned by the Wawasan Tani Cooperative. Drones are used to apply pesticides or fertilizers to plants. Observation results show that spraying pesticides using drones is more effective and efficient because it can spray pesticides to plants is represent to the plant and the processing time can be two until twenty times faster. In addition, drones have also been developed to determine the state of plant health and identify pests and diseases in the field.
Pendampingan Petani dalam Pengendalian Hama dan Penyakit Jambu Biji (Psidium guajava L.) di Desa Cibening, Kecamatan Pamijahan, Bogor, Jawa Barat Ruomenson Dedi Jefri Bakara; Fitrianingrum Kurniawati
Jurnal Pusat Inovasi Masyarakat (PIM) Vol. 2 No. 1 (2020): Februari 2020
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1151.516 KB)

Abstract

The majority of people in Cibening, Sub-district Pamijahan work as farmers. Guava is the main commodity that has been cultivated. This village is also a contributor to Bogor Regency, which is the third largest guava producer in West Java province. However lately the production of guava in this area has decreased so that farmers suffered significant losses. This loss caused by a pest attack that occurs for a long time. The main pest and disease in this village are Helopeltis sp. and plant parasitic nematodes. Farmers in this village still do not understand in detail related how to manage pest and disease. The use of chemical pesticides is the only one control strategy for pest and disease. With these conditions, farmers need assistance to solve the problem. This activity was done through three stages, namely preliminary survey, socialization related to the introduction and control of pests and diseases of guava, and training of PGPR as a biological agent and planting Tagetes sp as refugia and nematicidal actvity. The ojective of this activity was to enable farmers to have the ability to solve pest and disease problems damaging guava, with proper control that takes into account all aspects, so farmers get more financial benefits and environmental sustainability in this village can be realized. Guava farmers in Cibening Village, Pamijahan are able to recognize and deal with pests and diseases that attack guava independently and farmers get more financial and environmental sustainability benefits.
Sosialisasi Pemilahan Sampah dan Pelatihan Pembuatan Kompos di Desa Luwijawa, Kecamatan Jatinegara, Kabupaten Tegal Rifqie Mardiansyah Purmadi; Rahmi Jamza; Sugeng Santoso; Fitrianingrum Kurniawati; Budi Nugroho; Candra Budiman
Jurnal Pusat Inovasi Masyarakat (PIM) Vol. 2 No. 4 (2020): Juni 2020
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (227.686 KB)

Abstract

Luwijawa Village is a village located in Jatinegara District, Tegal Regency, Central Java Province. The village which has an area of 257,285 ha, the majority of its residents work as farmers. Agriculture carried out is rice farming with rice and corn as the main crops, as well as plantation crops such as coffee and durian. Villagers still use chemical fertilizer as a nutrient provider for plants, as well as the problem of rubbish that is still burned or thrown away by residents. The activity of sorting waste and training on composting was carried out to educate the public, especially PKK women, on how to manage waste to compost fertilizer. The socialization is done by the lecture method. The speaker explained the material using a power point and continued with video playback. Participants can ask questions after the socialization activities have been completed. Socialization activities and compost making training were carried out on different days. Compost making training is conducted with residents from organic waste. Organic waste obtained from residents' kitchen waste is cut to pieces and mixed with bioactivators (EM- 4). The ingredients that have been mixed are then put into a bucket and tightly closed so that no air enters, this is done to maximize the work of the bioactivator in the fermentation process. Compost making training activities took place well, there were quite a lot of participants, with relatively high enthusiasm. This is evidenced by the many questions asked by participants in the series of activities. Participants have been able to distinguish organic and inorganic waste, and have the desire to make their own compost with materials available in the surrounding environment. Keywords: compost, garbage, socialization
Teknik Budi Daya Dan Karakteristik Talas Belitung (Xanthosoma sagittifolium) di RW 01 Kelurahan Situ Gede Reynatha Syafira Rizkiya; Fitrianingrum Kurniawati
Jurnal Pusat Inovasi Masyarakat (PIM) Vol. 2 No. 5 (2020): Juli 2020
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (496.061 KB)

Abstract

Taro is one of the most cultivated plant in Bogor. One of the area that routinely producing taro is Situ Gede. There are two types of taro that commonly cultivated, belitung’s taro (Xanthosoma sagittifolium) and bogor’s taro (Colocasia esculenta L.). In Situ Gede, they only cultivating belitung’s taro (Xanthosoma sagittifolium). Planting and cultivating taro is not hard but the uncorrect way to cultivate taro can cause the yield not at its maximum. It still need a proper way of cultivation to gain maximum yield. This writing aims to know the cultivation technique and the characteristic of belitung taro (Xanthosoma dagittifolium) which is cultivated by the community of RW 01, Situ Gede village. This activity held in RW 01 Situ Gede on 19 of February 2020 to 3 of March 2020. The data’s taken with interviewing and observing the real situation. The data will be compared to literature with the same topic. The process of taro cultivation in Situ Gede village starts from the preparation of planting material which is propagated by itself from tubers, making beds, organic and inorganic fertilizing with different intervals, weeding and land disposal process for the purpose of production. Taro saplings are not discarded but are used for taro propagation. In general, the cultivation techniques applied in Situ Gede are in accordance with the literature and some taro farming practices in other areas. The number of tubers produced is largely influenced by environmental factors, spacing, and intensity of care in the cultivation process. Keywords: belitung’s taro, characteristic, cultivation, tuber
Identifikasi berbasis karakter molekuler Nucleopolyhedrovirus pada larva Helicoverpa armigera Hubner (Lepidoptera: Noctuidae) asal Bogor, Jawa Barat R. Yayi Munara Kusumah; Trendy Hartanto; Fitrianingrum Kurniawati
Jurnal Entomologi Indonesia Vol 19 No 2 (2022): July
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.19.2.127

Abstract

Nucleopolyhedrovirus (NPV) is a potential biological control agent recommended to control corn cob borer larvae (Helicoverpa armigera Huber). NPV characters can be studied using various detection and identification methods. One technique to study the character of NPV is polymerase chain reaction (PCR) followed by analysis of the results of nucleotide and amino acid sequences. The molecular character of NPV in infected H. armigera larvae in corn plantations in Cibeureum Village, Dramaga District, Bogor Regency using DNA polymerase partial gene sequences needs to be done. The methods used to study the character of the NPV consisted of DNA isolation using a modified CTAB method, partial gene amplification of DNA polymerase using HearNPVF1 and HearNPVR1, and analysis of the level of homology of nucleotide and amino acid sequences compared to other countries and phylogeny. PCR amplification using specific primers was successfully carried out with the HearNPV DNA polymerase partial gene DNA band measuring about 1,200 bp. Phylogenetic analysis was also successfully carried out and showed that there was a high relationship between HearNPV isolates from Indonesia (Bogor, West Java) and NPV isolates that infect Helicoverpa from other countries such as: Spain, Australia, the Netherlands, India, Brazil, Russia, and China with nucleotide and amino acid homology values ​​of 99%. HearNPV isolates from Bogor were in the same group as NPVs that attacked the Genus Helicoverpa from other countries, while NPVs from other genera were in separate groups based on phylogenetic analysis using Mega 7 software.
Biologi ngengat Galleria mellonella (Linn.) (Lepidoptera: Pyralidae) pada beberapa jenis pakan buatan Nadzirum Mubin; Mohammad Riza Krisnadi; Teguh Santoso; Fitrianingrum Kurniawati
Jurnal Entomologi Indonesia Vol 19 No 3 (2022): November
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.19.3.243

Abstract

The greater wax moth (Galleria mellonella (Linnaeus)) is one of Asia’s most essential pests in honey beekeeping. Apart from being a pest, G. mellonella was also used as a test insect in several business fields. The needs of the population of G. mellonella encourage several areas of the business to cultivate it practically and economically. This study aimed to determine and examine the composition of food ingredients as an artificial diet that can affect the growth and development of G. mellonella. The test consisted of four different feed-modified formulas, namely P1 (corn flour, wheat, glycerol, and yeast), P2 (rice flour, wheat flour, wheat bran, glycerol, and yeast), P3 (wheat flour, wheat, glycerol, and yeast), and P4 (brown rice, glycerol, and yeast). The observation criteria were life cycle, mortality rate, fecundity, and egg fertility of the G. mellonella moth. Based on the study’s results, the life cycle development of G. mellonella was the shortest in the P3 treatment (71.5 ± 5.2 days) and the longest P4 treatment (84.2 ± 8.3 days). The highest mortality rate was in the P3 feed type at 13.12%, while the lowest occurred in the P4 treatment at 4.44%. Based on the fecundity of female imago, treatment P4 had the highest fecundity level of 527.7 eggs/female, while treatment P1 was the lowest (169.6 eggs/female). P4 treatment responded best to several biological aspects of the G. mellonella moth, such as larger body size, lower mortality, and high fecundity rates.
Analisis filogenetik Hyposidra talaca nucleopolyhedrovirus (HytaNPV) yang diisolasi dari perkebunan teh Gunung Mas, Bogor, Jawa Barat dan virulensinya terhadap Hyposidra talaca Walker R Yayi Munara Kusumah; Fitrianingrum Kurniawati; Eka Dana Kristanto; Franciskus Parasian; Michael Christian
Jurnal Entomologi Indonesia Vol 20 No 2 (2023): July
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.20.2.151

Abstract

Hyposidra talaca (Walker) is an important pest of tea plant. H. talaca can cause losses of between 40–100% in the dry season if proper control is not carried out. H. talaca has natural enemies such as predators, parasitoids, and pathogens. One of the entomopathogens is NPV. This study aims to obtain molecular characteristics through DNA polymerase sequences and determine the virulence level of NPV isolates from H. talaca. The same species from different locations can have genetic variability. Therefore, molecular characterization by polymerase chain reaction (PCR) on DNA polymerase sequences is one way to study the genetics of HytaNPV. NPV was isolated from infected H. talaca larvae collected from the field. The DNA isolates were used as templates for PCR for DNA polymerase gene amplification with an amplicon target of ±1,000 bp. A sequencing process followed the PCR provides nucleotide sequence. HytaNPV DNA polymerase sequencing results were aligned with GenBank's BLAST data to provide information on the relationship of HytaNPV to NPVs isolated from other regions. Based on molecular character analysis using DNA polymerase gene sequence, HytaNPV Bogor has a homology level of 93.9% with HytaNPV isolated from India. HytaNPV Bogor has a genetic relationship with the NPV that infects Buzura suppressaria from China and Australia. HytaNPV Bogor is similar to the NPV that infects H. talaca from India. The bioassay of HytaNPV isolate against H. talaca showed the highest LT50 value of 1.92 days was found in concentration of 1.58 x 107 POBs/ml in second instar larvae.
Co-Authors Abdul Muin Adnan Abdul Munif Ade Indra Maulana Sembiring AHMAD JUNAEDI Anindita, Devina Cinantya Aris Budiman Ayang Ratna Kumala Baran Wirawan Cahyana, Destika Candra Budiman Charles Parningotan Haratua Simanjuntak Christian, Michael Dwi Rachmina Efi Toding Tondok Eka Dana Kristanto Faqih Udin Fawwaz El Auly Franciskus Parasian Gede Suastika Giyanto . GUSMAINI GUSMAINI, GUSMAINI HAJRIAL ASWIDINNOOR Hamidi, Ilmi Hana Khoirunisa Heriyanto Syafutra Hishar Mirsam Istikorini, Yunik Kikin H Mutaqin Kristanto, Eka Dana Kusumah, Yayi Munara Laksono Trisnantoro Maulidiya, Sherly Eka Michael Christian Mohammad Riza Krisnadi Muhammad Alam Firmansyah Muhammad Prama Yufdy, Muhammad Prama Muhammad Syukur Nadzirum Mubin Nadzirum Mubin Neng Tipa Nursipa Ni Wayan Kartika Pratiwi Parasian, Franciskus Pramudita, Pajar R Yayi Munara Kusumah Rahardjo, Budhi Rahmi Jamza Ramadhani, Ahmad Revi, Lestia Reynatha Syafira Rizkiya Rifqie Mardiansyah Purmadi Rizky Mailani Rahman Rosyid Amrulloh Ruomenson Dedi Jefri Bakara Sitanggang, Della SRI HENDRASTUTI HIDAYAT Supramana Syakir, Andi Muhammad Teguh Santoso Trendy Hartanto Zahra, Sabrina Aulia