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All Journal Jurnal Fitopatologi Indonesia Innofarm Jurnal Inovasi Pertanian Agrovigor: Jurnal Agroekoteknologi Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Entomologi Indonesia Jurnal Pusat Inovasi Masyarakat
Fitrianingrum Kurniawati, Fitrianingrum
Departemen Proteksi Tanaman, Fakultas Pertanian, IPB University, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Medicine & Pharmacology Social Sciences

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Pelatihan Pembuatan Pupuk Hayati di Desa Gondel, Kecamatan Kedungtuban, Kabupaten Blora, Jawa Tengah Firmansyah, Muhammad Alam; Kurniawati, Fitrianingrum; Mubin, Nadzirum; Istikorini, Yunik
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 3 (2024): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.10.3.305-314

Abstract

The rice productivity in Gondel Village, Kedungtuban District, Blora Regency, Central Java, has declined. Factors such as attacks by plant pests and diseases (OPT), climate change (DPI) impact, and decreasing soil quality are the leading causes of crop failure. This training aims to enhance the understanding and skills of Gondel Village farmers in making biofertilizers and introducing environmentally friendly pest and disease control methods. The activity involved 40 farmers from 8 farmer groups (Gemah Ripah, Margo Mulyo, Ngudi Makmur, Sido Dadi, Sido Makmur, Tambah Makmur, Tambah Rizki, and Sri Mulyo), with each group represented by five farmers. The training methods included analysis of soil chemicals, socialization, practical propagation of Trichoderma sp. on rice media, and biofertilizer production. Pre-test and post-tests were conducted to assess the improvement in farmers' knowledge. Trichoderma biofertilizer was formulated using carriers such as a) Manure and b) Compost, zeolite, and humic acid. The results of the soil chemical properties analysis showed that farmers' use of chemical fertilizers was very intensive, resulting in very high levels of phosphorus (P) and calcium (Ca) and high levels of potassium (K), magnesium (Mg), and cation exchange capacity (KTK). Evaluation results indicated that the training significantly improved the farmers' knowledge of biofertilizer production in theory and practice. However, some farmers still need further guidance on the propagation of the biological agent Trichoderma sp.
Analisis filogenetik Hyposidra talaca nucleopolyhedrovirus (HytaNPV) yang diisolasi dari perkebunan teh Gunung Mas, Bogor, Jawa Barat dan virulensinya terhadap Hyposidra talaca Walker Kusumah, R Yayi Munara; Kurniawati, Fitrianingrum; Kristanto, Eka Dana; Parasian, Franciskus; Christian, Michael
Jurnal Entomologi Indonesia Vol 20 No 2 (2023): July
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.20.2.151

Abstract

Hyposidra talaca (Walker) is an important pest of tea plant. H. talaca can cause losses of between 40–100% in the dry season if proper control is not carried out. H. talaca has natural enemies such as predators, parasitoids, and pathogens. One of the entomopathogens is NPV. This study aims to obtain molecular characteristics through DNA polymerase sequences and determine the virulence level of NPV isolates from H. talaca. The same species from different locations can have genetic variability. Therefore, molecular characterization by polymerase chain reaction (PCR) on DNA polymerase sequences is one way to study the genetics of HytaNPV. NPV was isolated from infected H. talaca larvae collected from the field. The DNA isolates were used as templates for PCR for DNA polymerase gene amplification with an amplicon target of ±1,000 bp. A sequencing process followed the PCR provides nucleotide sequence. HytaNPV DNA polymerase sequencing results were aligned with GenBank's BLAST data to provide information on the relationship of HytaNPV to NPVs isolated from other regions. Based on molecular character analysis using DNA polymerase gene sequence, HytaNPV Bogor has a homology level of 93.9% with HytaNPV isolated from India. HytaNPV Bogor has a genetic relationship with the NPV that infects Buzura suppressaria from China and Australia. HytaNPV Bogor is similar to the NPV that infects H. talaca from India. The bioassay of HytaNPV isolate against H. talaca showed the highest LT50 value of 1.92 days was found in concentration of 1.58 x 107 POBs/ml in second instar larvae.
Co-Authors Abdul Muin Adnan Abdul Munif Ade Indra Maulana Sembiring AHMAD JUNAEDI Anindita, Devina Cinantya Aris Budiman Ayang Ratna Kumala Baran Wirawan Cahyana, Destika Candra Budiman Charles Parningotan Haratua Simanjuntak Christian, Michael Dwi Rachmina Efi Toding Tondok Faqih Udin Fawwaz El Auly Gede Suastika Giyanto . GUSMAINI GUSMAINI, GUSMAINI HAJRIAL ASWIDINNOOR Hamidi, Ilmi Hana Khoirunisa Heriyanto Syafutra Hishar Mirsam Istikorini, Yunik Kikin H Mutaqin Kristanto, Eka Dana Kusumah, R Yayi Munara Kusumah, Yayi Munara Laksono Trisnantoro Maulidiya, Sherly Eka Mohammad Riza Krisnadi Mubin, Nadzirum Muhammad Alam Firmansyah Muhammad Prama Yufdy, Muhammad Prama Muhammad Syukur Nadzirum Mubin Neng Tipa Nursipa Ni Wayan Kartika Pratiwi Parasian, Franciskus Pramudita, Pajar Rahardjo, Budhi Rahmi Jamza Ramadhani, Ahmad Revi, Lestia Reynatha Syafira Rizkiya Rifqie Mardiansyah Purmadi Rizky Mailani Rahman Rosyid Amrulloh Ruomenson Dedi Jefri Bakara Sitanggang, Della SRI HENDRASTUTI HIDAYAT Supramana Syakir, Andi Muhammad Teguh Santoso Trendy Hartanto Zahra, Sabrina Aulia