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All Journal JURNAL PETERNAKAN Jurnal Zarah Jurnal Kesehatan Medika Saintika JURNAL ILMIAH PETERNAKAN TERPADU Majalah Kedokteran Andalas Jurnal Abdi Insani SPEKTA (Jurnal Pengabdian Kepada Masyarakat : Teknologi dan Aplikasi) Bioscientist : Jurnal Ilmiah Biologi Jurnal Ilmiah Wahana Pendidikan Jurnal Kesehatan Saintika Meditory Jurnal Peternakan Borneo Jurnal Medisains Kesehatan
Heppy Setya Prima
Stikes Syedza Saintika
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Karakterisasi dan Identifikasi Molekuler Bakteri Asam Laktat Diisolasi dari Ikan Bilih (Mystacoleucus padangensis) Danau Singkarak Berpotensi Sebagai Probiotik Prima, Heppy Setya
Majalah Kedokteran Andalas Vol. 46 No. 3 (2023): Online Juli 2023
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v46.i4.p535-547.2023

Abstract

Tujuan dari penelitian ini adalah untuk mengisolasi dan mengidentifikasi isolat bakteri asam laktat dari ikan inaktif (Mystacoleucus padangensis) dari Danau Singkarak Bilih dan mengevaluasi potensi probiotiknya. Setiap jenis bakteri asam laktat memiliki efek probiotik yang berbeda. Oleh karena itu, seleksi dan identifikasi diperlukan untuk mendapatkan strain probiotik yang baik. Tujuan dari penelitian ini adalah untuk mengetahui bakteri asam laktat yang terkandung dalam billfish tertentu (Mystacoleucus padangensis) melalui pengujian biokimia dan dilanjutkan melalui pengujian molekuler. Metode: Penelitian ini merupakan penelitian kualitatif deskriptif untuk mengetahui bakteri asam laktat tertentu pada ikan bilih (Mystacoleucus padangensis). BAL dilakukan menggunakan media isolasi selektif Man ROGOSA Sharpe Agar. Seleksi dilakukan dengan pengamatan morfologi dan pewarnaan Gram. Sifat biokimia dan jenis fermentasi juga diuji. Untuk mewujudkan potensinya sebagai probiotik, hasilnya adalah: Metode identifikasi molekuler menggunakan gen penanda 16S rRNA digunakan dalam identifikasi bakteri asam laktat pada penelitian ini. Berdasarkan hasil analisis dengan menggunakan BLAST, ditemukan bahwa jenis isolat bakteri fermentasi Ikan Bilih (Mystacoleucus padangensis) kode sampel IB1, 99,86% mirip dengan Lactobacillus fermentum 
Efek Dosis Probiotik Konsorsium pada Kualitas Organoleptik Daging Broiler Susalam, Malikil Kudus; Marlida, Yetti; Prima, Heppy Setya; Fajri, Fadhli; Maulana, Fajri
Jurnal Peternakan Vol 22, No 2 (2025): September 2025
Publisher : State Islamic University of Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/jupet.v22i2.33056

Abstract

ABSTRAK. Probiotik konsorsium merupakan kombinasi dari berbagai mikroorganisme yang saling bekerja sehingga menghasilkan manfaat yang lebih baik dibandingkan probiotik tunggal. Tujuan penelitian adalah untuk mendapatkan dosis pemberian probiotik konsorsium yang terbaik dilihat dari uji organoleptik daging broiler meliputi tekstur, rasa, warna dan aroma. Penelitian menggunakan ayam broiler yang dipelihara selama 35 hari dengan pemberian level probiotik konsorsium berbeda dalam air minum. Pakan yang digunakan adalah BR 1 Wonokoyo. P0 = pemberian level 0 ml probiotik konsorsium, P1 = pemberian level 0,5 ml probiotik konsorsium, P2 = pemberian level 1 ml probiotik konsorsium dan P3 = pemberian level 1,5 ml probiotik konsorsium dengan 5 kali pengulangan. Parameter yang diamati adalah tekstur, rasa, warna dan aroma daging. Hasil penelitian ini menunjukkan pemberian probiotik konsorsium pada ayam broiler memberikan pengaruh berbeda sangat nyata (P<0,01) terhadap tekstur daging, rasa daging, warna daging dan aroma daging. Hasil penelitian ini disimpulkan pemberian level 1 ml probiotik konsorsium pada ayam broiler diperoleh tekstur daging 4,40; rasa daging 4,31; warna daging 3,82 dan aroma daging 4,14.Kata kunci: Probiotik, konsorsium, tekstur, rasa, warnaThe Effect of Probiotic Consortium Dosage on the Organoleptic Quality of Broiler MeatABSTRACT. Consortium probiotics are a combination of various microorganisms that work together to produce better benefits than single probiotics. This research aimed to obtain the best dose of probiotic consortium based on organoleptic tests of broiler meat, including texture, taste, color, and aroma. This study used broiler chickens that were reared for 35 days by administering different levels of probiotic consortium in drinking water. The feed used is BR 1 Wonokoyo. P0 = Giving probiotic consortium level 0 ml, P1 = Giving probiotic consortium level 0.5 ml, P2 = Giving probiotic consortium level 1 ml, and P3 = Giving probiotic consortium level 1.5 ml with 5 times covered. The parameters observed were texture, taste, color, and aroma of meat. The results of this study showed that giving a probiotic consortium to broiler chickens had a very significantly different effect (P<0.01) on meat texture, meat taste, meat color, and meat aroma. From the results of this study, it was concluded that by administering 1 ml of the probiotic consortium to broiler chickens, the meat texture was 4.40, the meat taste was 4.31, the meat color was 3.82, and the meat aroma was 4.14.
Uji Aktivitas Antifungi Kombinasi Ekstrak Etanol Kulit Pisang Kepok (Musa paradisiaca L.) dan Kulit Jeruk Manis (Citrus sinensis) terhadap Candida albicans Penyebab Keputihan Patologis Secara In Vitro Yulia, Inelvi; Prima, Heppy Setya
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9360

Abstract

Pathological vaginal discharge (Candidiasis) is a reproductive health problem experienced by more than 50% of women throughout the world. Candida albicans is the main cause of pathological vaginal discharge. This disease is characterized by yellow to greenish discharge, odor, and itching to the point of bleeding which can cause death. Antifungal drugs used to treat pathological vaginal discharge can have several side effects. Therefore, the urgency of this research is to find natural alternatives for pathological vaginal discharge drugs, such as sweet orange peel and kepok banana peel which contain bioactive compounds that can inhibit the growth of Candida albicans. The aim of this study was to test the antifungal activity of a combination of ethanol extracts of sweet orange peel and kepok banana peel against Candida albicans in vitro. This research uses laboratory experimental methods. The results showed that all combinations of ethanol extracts of sweet orange peel and kepok banana peel could inhibit the growth of Candida albicans. The inhibitory power of each extract combination is categorized as moderate to very strong. According to the one-way ANOVA statistical test, all combinations were significantly different (p < 0.05). The ethanol extract of sweet orange peel contains flavonoids, alkaloids, phenolics and steroids, while the kepok banana peel extract contains phytochemical compounds such as flavonoids, phenolics, triterpenoids and alkaloids.
Characterization of Lactic Acid Bacteria from Budu Fish as Probiotic Candidates from Padang Pariaman Regency Prima, Heppy Setya; Kudus Susalam, Malikil Alam; Maulana, Fajri Fajri; fajri, fadhli fadhli
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 13 No. 2 (2025)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v13i2.p479-499

Abstract

West Sumatra is an area that has a large number of fermented food products, one of which is fermented fish called Budu. The purpose of this study was to isolate and identify Lactid Acid Bacteria isolates from Budu fish from Padang Pariaman Regency and evaluate their probiotic potential. The research method is as follows: isolation of Lactid Acid Bacteria from Budu fish, followed by assessment of resistance tests to gastric pH and bile salts and antimicrobial activity. Then, 16S rRNA was used to assess the species of lactic acid bacteria isolates. Of the 10 samples, one sample of Isolate MKS4, with potential antimicrobial activity was reported. The results showed that the morphological and biochemical characteristics of Lactid Acid Bacteria include Gram-positive bacteria, bacilli and catalase negative included in the homofermentative bacteria group. The greatest antimicrobial activity was shown by Escherichia coli MKS4 0157:H7 (14.79 mm), S. aureus ATCC 25923 (14.89 mm) and enteritidis ATCC 13076 (16.64 mm). The results of molecular identification using 16S rRNA showed that the isolated lactic acid bacteria had similarities with the Lactobacillus strain parabucneri strain HBUAS56106, namely antimicrobial potential against pathogenic bacteria. Lactobacillus. parabucneri strain HBUAS56106 can be used as an antidiarrheal agent and the best antimicrobial compound is classified as a probiotic bacteria
SELIMUT ANTI API INOVASI PENCEGAHAN DAN MITIGASI KEBAKARAN TAHAP AWAL BERSAMA MITRA PBKM KESUMA MARELAN MEDAN Suyanti, Retno Dwi; Pardosi, Sri Masnita; Nasution, Alvi Sahrin; Prima, Heppy Setya
Jurnal Abdi Insani Vol 12 No 11 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i11.2838

Abstract

Fires are sudden disasters that can cause significant losses, both material and non-material. Prompt early response is crucial to prevent the spread of fire, but many communities lack simple and easy-to-use fire extinguishers. A five-member community service team, in collaboration with the Kesuma Community Learning Center (PKBM), led by Ms. Siti Nurhayati, located in Lk 2 Pasar 2 Barat Terjun, Medan Marelan, and involving 40 community members as partners, conducted training and developed an innovation called a "Fulerene Patterned Fire Blanket." This blanket is made from heat-resistant fullerene-patterned fiberglass and coated with sodium bicarbonate (NaHCO₃), which accelerates fire extinguishing by lowering the temperature and inhibiting oxidation. This combination of materials results in a device that is high-temperature resistant, lightweight, and reusable after washing. This activity included outreach, demonstrations, hands-on training, and involved the local community in simple production of the device. The results demonstrated an increase in community knowledge and skills in dealing with small fires. Enthusiasm for this safe and economical design has also increased disaster awareness and preparedness at the community level. The positive response has opened up opportunities for broader program development. Scientific studies have shown that fullerene-patterned fire blankets are effective as a preventative solution, enhancing community preparedness for early fire risks.
Integrated Aeroponic Technology for Enhancing Community-Based Potato Seed Production in North Sumatra Diningrat, Diky Setya; Harahap, Novita Sari; Prima, Heppy Setya; Febriyossa, Adelia; Maulana, Bagoes; Sibuea, Abdul Muin; Sari, Laela; Jamaludin, Askiah Binti
SPEKTA (Jurnal Pengabdian Kepada Masyarakat : Teknologi dan Aplikasi) Vol. 6 No. 2 (2025)
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/spekta.v6i2.13927

Abstract

Background: Indonesia's potato production faces critical challenges in quality seed provision. Traditional G0 seed propagation methods achieve only 40-60% survival rates due to transplant shock and environmental stress. Contribution: This research contributes to community-based technology transfer literature in agricultural seed systems by implementing integrated aeroponic-micro tuber technologies through participatory partnerships. Method: A participatory action research approach was employed over four months (January-April 2025) involving 25 participants from Gapoktan Nilam farmer group in North Sumatra. The tri-partite partnership included farmers, PT. G10 Agrotech, and Universitas Negeri Medan. Training modules covered aeroponic fundamentals, micro-tuber applications, and integrated system management. post assessments measured survival rates, productivity, knowledge, and skills using paired t-tests. Results: Survival rates improved significantly from 52.3±8.2% to 83.7±4.1% (p<0.001). Monthly seedling production increased by 171% from 2,040±320 to 5,520±280 units. Participant knowledge scores doubled from 4.2±0.8 to 8.5±0.6 (p<0.001). The program achieved 95% participation with 78% satisfaction rates and demonstrated 240% return on investment within 12 months. Conclusion: The integrated approach effectively addresses transplant shock while establishing sustainable community partnerships. This model demonstrates successful scaling of complex agricultural technologies at grassroots level, contributing to enhanced food security through improved seed systems.
Uji Aktivitas Antibakteri Ekstrak Kulit Jeruk Jesigo (Citrus Nobilis Lour) Terhadap Bakteri Escherichia Coli dan Listeria Monocytogenes Prima, Heppy Setya; Yansen, Fatridha
Jurnal Zarah Vol. 12 No. 1 (2024): April, 2024
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Maritim Raja Ali Haji

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31629/96x3t956

Abstract

Listeria monocytogenes dan Escherichia  coli adalah bakteri penyebab berbagai infeksi pada manusia. Kulit jeruk JESIGO  (Citrus nobilis Lour) mengandung  senyawa kimia berupa flavonoid,  alkaloid,  saponin  dan  tanin yang  berfungsi  sebagai  antibakteri. Penelitian  ini  bertujuan  untuk  mengetahui  aktivitas ekstrak kulit jeruk JESIGO  (Citrus nobilis Lour) dalam menghambat pertumbuhan bakteri Escherichia coli dan  Listeria monocytogenes.  Penelitian ini merupakan eksperimental laboratorium  dengan metode  difusi  agar untuk mengetahui daya hambat terhadap bakteri Escherichia coli dan  Listeria monocytogenes melalui pembentukan zona inhibisi. Sampel  yang  digunakan adalah bakteri Escherichia coli dan  Listeria monocytogenes. Beberapa variasi konsentrasi ekstrak kulit jeruk JESIGO; 5%, 15%, 25%,  45%, digunakan dalam penelitian ini.  Amoxicilin  dan DMSO digunakan sebagai kontrol positif dan kontrol negatif. Berdasarkan hasil penelitian,  konsentrasi ekstrak kulit jeruk JESIGO konsentrasi 45% menunjukkan daya hambat terbaik terhadap E. coli O157 dengan diameter zona hambat sebesar 15,76 mm (kuat). Diameter zona hambat terbesar terhadap bakteri Listeria monocytogenes diperoleh dari ekstrak kulit jeruk JESIGO dengan kosentrasi 45% yaitu 19,98 mm (kuat). Hasil penelitian menunjukkan bahwa ekstrak kulit jeruk JESIGO  (Citrus nobilis Lour)  memiliki aktivitas antibakteri terhadap Escherichia coli dan  Listeria monocytogenes dalam kategori kuat.
Perbandingan Aktivitas Anti-Hiperurisemia Ekstrak Etanol Seledri (Apium Graveolens L.) Dan Daun Salam (Syzygium Polyanthum) Pada Mencit Yang Diinduksi Kafein Yansen, Fatridha; Putra, Nanda; Gusnelti, Yesi; Prima, Heppy Setya
Jurnal Zarah Vol. 13 No. 1 (2025): April, 2025
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Maritim Raja Ali Haji

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31629/zarah.v13i1.6984

Abstract

Celery plants (Apium Graveolens L) and bay leaves (Syzygium polyanthum) have been used as alternative medicine to treat Hyperuricemia. This study aimed to determine the effectiveness of administration of extracts from celery plants and bay leafs to decrease uric acid levels in blood. This research used a post-test control group only design. A total of 24 male mice (randomly divided into 4 groups; P0, P1, P2 and P3). P0 was the control group (induced with caffeine at a dose of 0.5 mg/kgBW). P1 was induced with caffeine 0.5 mg/kgBW+celery extract 35 mg/kgBW, P2 was induced by caffeine 0.5 mg/kgBW+bay leaf extract 35 mg/kgBW and P3 was induced by caffeine + a combination of celery plant extract and bay leaves 35mg/kgBW. Uric acid levels were measured using an easy touch GCU meter device. The results showed that average of uric acid level for P0, P1, P2 and P4 were 7.1mg/dl; 4.9 mg/dl; 4 mg/dl; and 5.2 mg/dl, respectively. The most significant antihyperuricemia activity was demonstrated by administering 35 mg/kgBW of bay leaf extract. The One Way ANOVA analysis described that the administration of extract ethanol generated significant effects (p-value <0.05). Based on post hoc LSD analysis, it was found that the administration of ethanol extract from bay leaves showed significant differences compared to combination (celery + bay leaves) in lowering uric acid levels. Therefore, it was concluded extract from celery and bay leaves can be used to lower the level of uric acid
KARAKTERISASI DAN IDENTIFIKASI MOLEKULER BAKTERI ASAM LAKTAT ASAL IKAN RINUAK (PSILOPSIS SP) SEBAGAI KANDIDAT PROBIOTIK HALAL DARI DANAU MANINJAU SUMATERA BARAT Annita Annita; Eliza Zahra; Heppy Setya Prima
Jurnal Kesehatan Medika Saintika Vol 16, No 1 (2025): Juni 2025
Publisher : Stikes Syedza Saintika Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30633/jkms.v16i1.2976

Abstract

Lactic Acid Bacteria (LAB) have recently become one of the main topics of discussion in the fields of health, food industry, science, animal husbandry, agriculture. LAB plays an important role in efforts to reduce stunting through various mechanisms related to intestinal health and nutrient absorption. One source of LAB is fermented Rinuak fish (Psilopsis sp) from Lake Maninjau, West Sumatra, Indonesia, which is isolated from probiotic bacteria which have the potential to act as antimicrobials, but must be ensured that they are halal in accordance with Islamic religious teachings. The aim of this study was to examine the antimicrobial potential of probiotic LAB isolated from Rinuak fish (Psilopsis sp). The research methods carried out were as follows: isolation and characterization of Rinuak fish (Psilopsis sp) LAB DNA, followed by assessment of the antimicrobial activity of the raw bacteriocin supernatant. Then, 16S rRNA was used to assess LAB isolate species that potentially had antimicrobial activity. The results showed that 3 samples of Rinuak fish (Psilopsis sp), After testing its ability to kill pathogenic bacteria, it was found that IR1 isolate had the greatest antimicrobial activity against Escherichia coli ATCC 0157 (30..27 mm), Staphylococcus aureus ATCC 25923 (25.65 mm) and Listeria monocytogenes CFSAN 004330 (18.65 mm) while the diameter of the inhibition zone by the supernatant of lactic acid bacteria Crude bacteriocin against Escherichia coli ATCC 0157 was 18.87 mm, Staphylococcus aureus ATCC 25923 was 22.67 mm while Listeria monocytogenes CFSAN 004330 did not form an inhibition zone. The results of molecular identification using 16S rRNA showed that the isolated lactic acid bacteria were 99.64% similar to those isolated from Rinuak fish (Psilopsis sp) with Lentilactobacillus buchneri subsp. silagei CD034 strain 17-16, which has antimicrobial potential against pathogenic bacteria. Lentilactobacillus buchneri subsp. silagei CD034 strain 17-16 can be used as an antidiarrheal and antityphoid drug in humans as well as a natural preservative in food that is guaranteed halal
ANALISIS BIOETIKA TERHADAP PRAKTIK PENGELOLAAN BIOPESTISIDA DALAM AGROWISATA PT. MEREK INDAH LESTARI (TAMAN SIMALEM RESORT) Adinda Laura Munthe; Beatreak Banjarnahor; Desi Arzeti Nainggolan; Rani Simanjuntak; Widyanti Saragi; Eko Prasetya; Aswarina Nasution; Heppy Setya Prima
Jurnal Ilmiah Wahana Pendidikan Vol 12 No 8.B (2026): Jurnal Ilmiah Wahana Pendidikan
Publisher : Peneliti.net

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Agrowisata dapat diartikan sebagai gabungan antara aktivitas pertanian dan pariwisata yang tidak hanya memberikan pengalaman liburan bagi pengunjung, tetapi juga berfungsi sebagai sarana pembelajaran tentang praktik pertanian yang berkelanjutan. Salah satu langkah yang diambil untuk mendukung sistem pertanian yang ramah lingkungan adalah dengan menerapkan biopestisida sebagai solusi alternatif untuk mengendalikan hama. Tujuan dari penelitian ini adalah untuk mengevaluasi pengelolaan biopestisida di wilayah agrowisata PT. Merek Indah Lestari (Taman Simalem Resort) dengan menggunakan sudut pandang bioetika. Penelitian ini menerapkan pendekatan deskriptif kualitatif dengan menggunakan metode studi kasus yang dilaksanakan di Organic Research Center pada zona 1, zona 2, dan zona 8. Pengumpulan data dilaksanakan melalui observasi partisipatif selama kegiatan magang, serta didukung oleh tinjauan pustaka dari beragam sumber ilmiah. Analisis dilakukan dengan berpijak pada prinsip-prinsip bioetika, yaitu beneficence (memberikan manfaat), non-maleficence (tidak menimbulkan kerugian), dan tanggung jawab terhadap keberlanjutan lingkungan. Temuan dari penelitian ini menunjukkan bahwa pemanfaatan biopestisida di lokasi tersebut membantu mendukung praktik pertanian yang lebih aman bagi lingkungan, manusia, dan organisme non-target.
Co-Authors Abdul Muin Sibuea Adelia Febriyossa Adinda Laura Munthe Agasi, Satri Yusasra Annita Annita Aswarina Nasution Beatreak Banjarnahor Befani Osanna Purba Dameria Sibarani Dara Betsaida Sipayung Dea Anzelika Desi Arzeti Nainggolan Dewi Sinta Wati Diningrat, Diky Setya Eko Prasetya Eliza Zahra Fadhli Fajri fajri maulana Falya Andini Fatiha Salma Siregar Fatridha Yansen Febrina, Bunga Putri Gracelda E. Pardede Gusnelti, Yesi Harahap, Novita Sari Ibrahim Ibrahim Inelvi Yulia Jamaludin, Askiah Binti laela Sari, laela Maulana, Bagoes Mawar Gultom Muhammad Amran Muthia Syahbana Nasution, Alvi Sahrin Nazlia Ramadani Tambunan Noviyanti, Aidha Nursakinah Harahap Nursyamsiah Harahap Oktaviani Sitohang Pardosi, Sri Masnita Pricilia, Adelva Putra, Nanda Raden Mohamad Herdian Bhakti Rani Simanjuntak Retno Dwi Suyanti Ruqaya annisa nurul haq Rusmawar Silaban Sandri, Dwi Sarah Amelia Gultom Selpiana Br Pasaribu Sofiana C.A Sihombing Susalam, Malikil Kudus Venni Handayani Widyanti Saragi Yansen, Fatridha Yetti Marlida Yulia Beti