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All Journal HAYATI Journal of Biosciences VALENSI Farmaka International Journal of Integrated Health Sciences Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Pharmaceutical Science and Technology Jurnal Farmasi Klinik Indonesia Pharmacology and Clinical Pharmacy Research FITOFARMAKA: Jurnal Ilmiah Farmasi Molekul: Jurnal Ilmiah Kimia JFIOnline Indian Journal of Forensic Medicine & Toxicology
Rizky Abdulah
Fakultas Farmasi Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Journal : HAYATI Journal of Biosciences

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Anticancer Activities and Metabolite Profiling of UHPLC-HRMS Method from Chrysanthemum x morifolium (Ramat.) Hemsl Leaves Maesaroh, Imas; Barliana, Melisa Intan; Abdulah, Rizky; Muhaimin, Muhaimin
HAYATI Journal of Biosciences Vol. 33 No. 1 (2026): January 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.1.167-180

Abstract

The Chrysanthemum morifolium Ramat, traditionally used for cancer treatment, including breast cancer, possesses anticancer properties. The aim of this study is metabolite profiling using ultra-high-performance liquid chromatography in conjunction with the high-resolution mass spectrometry (UHPLC-HRMS) technique and its correlation with the cytotoxic activity of the extract and ethyl acetate fraction of Chrysanthemum x morifolium (Ramat.) Hemsl leaves on cancer cells. The ethyl acetate fraction from the hydrolyzed ethanol extract of (Chrysanthemum x morifolium (Ramat.) Hemsl) leaves has anticancer activity against the MCF-7 breast cancer cells. Metabolite profiling was used to understand the presence of metabolites that have anticancer activity. UHPLC-HRMS was used to profile their metabolites. Compound Discoverer 3.3 software finished data processing and metabolite annotation. Anticancer activity was performed using the 2-[2-methoxy-4-nitrophenyl]-3[4-nitrophenyl]-5[2,4-disulfophenyl]-2H-tetrazolium (WST-8) assay. As many as 57 secondary metabolites were identified by UHPLC-HRMS analysis. Secondary metabolites that have the potential as anti-breast cancer are glycitein, diosmetin, kaempferol, esculetin, scopoletin, dihydroartemisinin, and Chrysin, with successive percentages of 31.39%, 19.91%, 5.61%, 2.63%, 0.82%, 0.14%, and 0.05%. Ethyl acetate fraction showed stronger cytotoxic activity than ethanol extract against MCF-7 cells with IC50 values of 66.31 ppm at 24 hours incubation and 40.35 ppm at 48 hours. Further research can be conducted on the isolation of flavonoids from the ethyl acetate fraction, as well as the analysis of cell cycle apoptosis stimulation and gene expression mechanisms.
Co-Authors . Supriyatna, . Ace Tatang Hidayat Agustini, Dewi D. Ahmad Faried Aisha K. Nur Ajeng Diantini, Ajeng Akhmad Priyadi Alvian, Sofa Dewi Ami A. Pratiwi Anas Surbanas Andreas Ciokan Angga Prawira Kautsar Arifah, Gina Asep Supriadin Barliana, Melisa I. Barliana, Melisa I. Carissa P. Purabaya Cherry Rahayu Cherry Rahayu Cherry Rahayu Cinthya, Sindy E. Ciokan, Andreas Desi Harneti Putri Huspa Destiani, Dika P. Desyandri Desyandri Devinna Kang Dewi D. Agustini Dika P. Destiani Dinge, Fonny Dyah A. Perwitasari Eka W. Suradji Eli Halimah, Eli Eli Mirdayani Emma Surahman Emma Surahman, Emma Eni Indrawati Ernestine Arianditha Pranasti Eva S. Tarigan Fajar, Mohamad Febriyanti, Maya Febriyanti, Maya Finisanti, Ratih Fonny Dinge Fonny Dinge Furqani, Winda H. Ghassani, Salma K. Gina Arifah Haafizah Dania Hanny Hafiar Hasriana Hasriana Hasriana Hasriana, Hasriana Hasrianna Hasrianna Hasrianna, Hasrianna Henry Ng Hersa Milawati Hersa Milawati Hilmi, Indah L. Hiroshi Koyama Imam A. Wicaksono Imaniar N. Faridah Imas Maesaroh Indah L. Hilmi Indah Laily Hilmi Indrawati, Eni Insani, Widya N. Irma M. Puspitasari Irma M. Puspitasari Irma M. Puspitasari, Irma M. Ivan S. Pradipta Ivan S. Pradipta Kang, Devinna Katherine A.Tjenggal Keri Lestari Khairinnisa, Miski A. Khansa F. Rahmah Kumamba, Raine D. Kusuma, Sri A. F. Leri Septiani Marline Abdassah, Marline Maya Febriyanti Meilani Jayanti Melisa I. Barliana Melisa I. Barliana Melisa I. Barliana Melisa I. Barliana Melisa Intan Barliana Milawati, Hersa Miski A. Khairinnisa Mohamad Fajar Mohamad Fajar Mohamad Nurul Azmi Mohamad Nurul Azmi bin Mohamad Taib Muhaimin Muhaimin Muhammad Rivai Mutakin Mutakin Nabilah Nadhif Nabilah Nadhif Ng, Henry Norisca A. Putri Nunung Kurniasih Nunung Kurniasih Nunung Kurniasih, Nunung NURUL ANNISA Nurul Annisa Nurul Annisa Okky S. Purwanti Pradipta, Ivan S. Pratiwi, Ami A. Priyadi, Akhmad Purabaya, Carissa P. Purwanti, Okky S. Rahayu, Cherry Rahayu, Fima Perdani Raine D. Kumamba Rano K. Sinuraya Rano K. Sinuraya Rano K. Sinuraya RANO KURNIA SINURAYA Ratih Finisanti Rika Yulianti, Rika Riska F Siregar Salma K. Ghassani SILVIA PERMATA SARI Sindy E. Cinthya Sinuraya, Rano K. Siregar, Riska F Sitepu, Annisa Ayu Ningtyas Carolina Siti Saidah Siti Saidah Sofa D. Alfian Sofa D. Alfian Sofa D. Alfian Sofa D. Alfian, Sofa D. Sofa Dewi Alfian Sri A. F. Kusuma Sri A. Sumiwi Sri M. Wahyuningrum Sumiwi, Sri A. Sunu Widianto Sunu Widianto Supriyatna Supriyatna Surachman, Emma Surachman, Emma Suradji, Eka W. Tarigan, Eva S. TIANA MILANDA Tina Rostinawati Unang Supratman Wahyuningrum, Sri M. Widianto, Sunu Widya N. Insani Winda H. Furqani Yudi Mulyana Hidayat Zulfan Zazuli