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Protective Effects of Red Spinach (Amaranthus tricolor L.) Ethanolic Extract on Tracheal Histopathology in Cigarette Smoke-Exposed Wistar Rats Setiawan, Haris; Agrisukma, Salsabilla Ari Sekar; Putra, Ichsan Luqmana Indra
Jurnal Sain Veteriner Vol 44, No 1 (2026): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.111588

Abstract

Cigarette smoke generates free radicals that can cause various respiratory disorders. Red spinach (Amaranthus tricolor L.) is a plant rich in antioxidants, which may help neutralize free radicals produced by cigarette smoke exposure. This study aimed to evaluate the protective effects of red spinach ethanol extract on the trachea of Wistar rats exposed to cigarette smoke. Twenty-four male Wistar rats were randomly assigned to four groups: K (distilled water), KN (cigarette smoke exposure), P1 (cigarette smoke exposure with 200 mg/kg BW extract), and P2 (cigarette smoke exposure with 400 mg/kg BW extract). Treatments were administered daily for 21 consecutive days. On day 22, the rats were euthanized, and their tracheas were collected for histological analysis. The tracheal tissues were processed using the paraffin embedding method and stained with hematoxylin and eosin. The evaluated parameters included epithelial cell height, tracheal lumen diameter, and goblet cell number. Data were analyzed using one-way ANOVA followed by Duncan’s post hoc test, with significance set at p < 0.05. Results demonstrated that exposure to cigarette smoke (KN group) significantly disrupted the tracheal histological structure (p < 0.05). However, administration of 400 mg/kg BW red spinach ethanol extract (P2 group) exhibited a protective effect by preserving epithelial cell height, lumen diameter, and goblet cell number comparable to those of the control group (K). In conclusion, red spinach ethanol extract at a dose of 400 mg/kg BW effectively protects the tracheal structure of Wistar rats against cigarette smoke-induced damage by maintaining epithelial cell height, lumen diameter, and goblet cell number at near-normal levels.
Pemantauan populasi ulat grayak jagung Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) pada pertanaman jagung dan padi menggunakan berbagai jenis perangkap: Monitoring population of fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) Smith (Lepidoptera: Noctuidae) in maize and rice fields using different types of trap Ihsan Nurkomar; Ichsan Luqmana Indra Putra; Dina Wahyu Trisnawati; Fadhila Lutfiyyatul Zahra; Achmad Fauzan Dharmawan
Jurnal Entomologi Indonesia Vol 23 No 1 (2026): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.23.1.1

Abstract

Spodoptera frugiperda Smith is a major invasive pest of maize and has also been reported attacking other host plants, including rice. As a migratory pest with high reproductive capacity, the population dynamics of S. frugiperda are influenced by cropping systems and host plant availability. Accurate population monitoring is a key component of integrated pest management (IPM). However, the performance of different trap types under varying agroecosystems has not been fully understood. This study aimed to evaluate the performance of different trap types (pheromone, light, and pheromone–light combination traps) in detecting S. frugiperda populations in maize and rice fields under different agroecosystem conditions. The study was conducted at four locations representing distinct agroecosystem conditions. Each location consisted of three field plots as independent replications. The number of adults captured per trap per week was used as an indicator of trap performance. The results showed that S. frugiperda populations were significantly higher in maize fields than in rice fields (p < 0.0001). A significant interaction was found between trap type and agroecosystem condition (p < 0.001). Pheromone traps recorded the highest captures under high population conditions, whereas light and combination traps performed relatively similarly under low population conditions. Light traps captured more non-target insects than pheromone traps. These findings indicate that the performance of S. frugiperda traps is context-dependent and should be adapted to the cropping system to support IPM-based monitoring strategies.
PARASITIZATION, PARASITISM, AND COMPARISON OF SEX RATIO OF Telenomus remus TO EGGS OF Spodoptera frugiperda ON CORN PLANTS IN KULON PROGO REGENCY Chiquita Almira; Ichsan Luqmana Indra Putra
Agrovigor Vol 19, No 1 (2026): In Progress
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v19i1.33082

Abstract

Spodoptera frugiperda is an invasive pest that causes severe damage to maize crops in Kulon Progo Regency. One biological control effort against this pest is the use of the egg parasitoid Telenomus remus. This study aimed to analyze the parasitization rate, parasitism rate, sex ratio, and their correlations in T. remus parasitizing S. frugiperda eggs in maize fields in Kulon Progo Regency. Egg samples were collected from maize fields using a W-pattern scouting method and reared under laboratory conditions until adult parasitoids or host larvae emerged. The number of parasitized eggs, successful parasitoid emergence, and the proportion of female and male individuals were recorded. The results showed that T. remus exhibited a relatively high parasitization rate, while the parasitism rate was moderate, with a female-biased sex ratio. Correlation analysis indicated that parasitization rate, parasitism rate, and sex ratio were not significantly correlated. These findings indicate that the developmental success of T. remus is influenced more by the biological condition of host eggs than by the number of eggs parasitized.
Effect of Calina Papaya Leaves Extract on Respiratory Tract in Cigarette Smoke Exposed Rats Haris Setiawan; Arlinda Sekar Ayu Hikmawati; Sri Wijayanti Wulandari; Eriza Via Malinda; Ichsan Luqmana Indra Putra
Jurnal Biodjati Vol 7 No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.19068

Abstract

Cigarette smoke is a source of free radicals that cause health problems throughout the world. Indonesia is a country that has many sources of natural antioxidants in counteracting free radicals, one of which is Calina papaya leaves. The study aimed to determine the potential of Calina papaya leaves antioxidants in improving the respiratory organs of Wistar rats after being exposed to cigarette smoke. The study using 25 male Wistar rats consisted of Control (not exposed to cigarette smoke and given distilled water), KN (cigarette smoke and given distilled water), P1 (cigarette smoke and Calina Papaya Leaves Ethanolic extracts (CPLE) 100 mg/kg BW), P2 (cigarette smoke and CPLE 200 mg/kg BW), and P3 (cigarette smoke and given CPLE 300 mg/kg BW). Exposure to cigarette smoke and administration of extracts was carried out for 21 days. On day 22, the rats were euthanized and dissected to remove the lungs and trachea. Organs were prepared using the paraffin method and hematoxylin-eosin staining. Parameters consisted of histopathological observations of the lungs and trachea. All data parameters were analyzed using a one-way ANOVA test and Duncan's advanced test (p<0.05). The results showed that there was an improvement in histopathology of the lungs and trachea at a dose of 200 mg/Kg BW compared to other doses (p<0.05). The study showed that the Calina papaya leaves ethanolic extracts have the potential as a source of antioxidants in improving the respiratory organs of Wistar rats after being exposed to cigarette smoke.
Incidence and Severity of Spodoptera frugiperda Attacks on Maize Crops in Kulon Progo District Amalina Nadhilah Izzuddin; Putra, Ichsan Luqmana Indra
PLANTA TROPIKA Vol. 14 No. 1 (2026)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v14i1.29362

Abstract

Spodoptera frugiperda is a pest that can rapidly destroy maize plants and cause economic losses for maize farmers. The objective of this study was to analyze the incidence and severity of S. frugiperda on maize crops in Kulon Progo District. Sampling locations were determined using the random stratified method. The purposive method was used to determine the maize fields used for sampling. The W-scouting method was used to calculate the incidence and severity of S. frugiperda infestation. The collected data were analyzed using a correlation test to evaluate the relationship between the incidence and severity of S. frugiperda infestation. The analysis revealed spatial variability in infestation levels. The lowest incidence was observed in Samigaluh Sub-district, with an infestation rate of 9.13%, whereas the highest was observed in Panjatan Sub-district, with a rate of 20.95%. The degree of crop damage also differed among locations. In Lendah Sub-district, the pest caused substantial injury, with 14 corn plants categorized as severely damaged. In contrast, Temon Sub-district recorded 176 plants exhibiting only minor damage symptoms. These results indicate that S. frugiperda infestations were present throughout the surveyed region, with the highest incidence detected in Panjatan and the lowest in Samigaluh, suggesting localized differences in pest pressure and environmental suitability.
Co-Authors AA Sudharmawan, AA Abda Abda Achmad Fauzan Dharmawan Agrisukma, Salsabilla Ari Sekar Ahmad Tantowi Akhmad Nizzar Nasikhudin Alfi Nurcahyasari Amalina Nadhilah Izzuddin Andika Setyo Budi Any Guntarti Aprilda, Clariza Ninda Aris Setiawan Arlinda Sekar Ayu Hikmawati Aulia Wulanda Aulia Wulanda Aulia, Dwi Ranti Azzahra, Fara Chiquita Almira Citra Ariani Edityaningrum, Citra Clariza Ninda Aprilda Codrat Dwi Nugroho Sulistyo Wati Daud, Intan Dede Maryana Dina Wahyu Trisnawati Dita Megasari Dwi Setyawan Eriza Via Malinda Fadhila Lutfiyyatul Zahra Fauzi Ahmad Muda Glady Sunggoro Haris Setiawan Husni dan Zulfanazli Pudjianto Alfian Rusdy Ihsan Nurkomar Ihsan Nurkomar Inggita Utami Irwanto Sucipto Khairurrizki, Amanda Khusnul Khotimah Khusnul Khotimah Kurnia Suci Maharani Laela Hayu Nurani Lalu Muhammad Irham Lenggar, Benta Listiatie Budi Utami Lyly Nur Indah Sari MA’RUFAH, NILA Maolinda Budiarti Misbahul Munir Moh. Mirza Nuryady Muhammad Izzuddin Noor Rahmadi Muhammad Usman Mulyono Mulyono Nanda Dwi Martina Nanda Dwi Martina Nina Restyaningsih Nisrina Az-Zahra Nurlaily Nur Ismiyati Nuri Dwi Astuti Nurul Istiqomah Nurul Suwartiningsih Nurwidodo, Nurwidodo Pertiwi, Pelangi Mayang Sukma Pratiwi, Ambar Pravitarani, Faradina Pudjianto Pudjianto Putra, Sigit Surya Putri, Aulia Ramanda Putri, Dila Savira Mayang Putri, Tasya Aulia Putri, Tasya Triana Raffly Muhammad Dhiaulhaq Ridwan Alfatah Rizki G. Pangestu Sadda Salisa Yahya Saputra, Wahyu Hana Sigit Surya Putra Siti Rofida Sri Wijayanti Wulandari Sri Wijayanti Wulandari Suci Resty Rahmadini Sugiyanto - Suprihatini, Novilia Suwartiningsih, Nurul Suwartiningsih, Nurul Syiama Nofitri Yuningsih Tazkiah Unida Tri Liana Marthadella Unida, Tazkiah Utami, Inggita Utami, Nurul Putrie Wikantyoso, Bramantyo Wildan Muhlison Winda Aulia Putri Wulandari, Sri Wijayanti Yahya Hanafi