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All Journal Indonesian Journal of Tropical and Infectious Disease Jurnal Pengabdian Kepada Masyarakat Universitas Bangka Belitung INFORMAL: Informatics Journal Jurnal RESTI (Rekayasa Sistem dan Teknologi Informasi) Promotif: Jurnal Kesehatan Masyarakat Jurnal Ilmiah Ilmu Kesehatan: Wawasan Kesehatan Jurnal Kesehatan Manarang Jurnal Sisfokom (Sistem Informasi dan Komputer) Jurnal Endurance: Kajian Ilmiah Problema Kesehatan SCIENTIA JOURNAL Infokes : Info Kesehatan Gorontalo Journal of Public Health Jurnal Kesehatan Jurnal Kesehatan Ilmiah Indonesia / Indonesian Health Scientific Journal Contagion: Scientific Periodical Journal of Public Health and Coastal Health Jurnal Kesmas (Kesehatan Masyarakat) Khatulistiwa Jurnal Ilmiah Manusia dan Kesehatan Jurnal Ners Jurnal Pesut : Pengabdian untuk Kesejahteraan Umat Kampurui Jurnal Kesehatan Masyarakat Miracle Journal Of Public Health (MJPH) Jurnal Promotif Preventif KESANS : International Journal of Health and Science Jurnal Puan Indonesia pendidikan, science, teknologi, dan ekonomi Ilmu Komputer untuk Masyarakat Public Health and Safety International Journal (PHASIJ) Riset Informasi Kesehatan JT-IBSI (Jurnal Teknik Ibnu Sina) Health Dynamics Jurnal Riset Sains dan Kesehatan Indonesia Jurnal Ilmu Kedokteran dan Kesehatan Indonesia Jurnal Kesehatan Ibnu Sina (j-kis)
Herdianti Herdianti
Fakultas Ilmu Kesehatan Universitas Ibnu Sina
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Journal : Health Dynamics

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Assessment of Knowledge, Attitude and Practice Regarding Biosafety among Laboratory Staff in a University HAMZAH, NURUL AINUN; Nik Mohd Razali, Nik Nur Fatin Athirah; Abdul Samad, Nurul Izzah; Mohd Amin, Nursuhaili; Yhaya, Mohd Firdaus; Hatta, Herdianti
Health Dynamics Vol 2, No 10 (2025): October 2025 (In progress)
Publisher : Knowledge Dynamics

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Abstract

Background: Biosafety is crucial for protecting laboratory staff from infectious agents and hazardous biological materials. Inadequate knowledge of unsafe practices may increase the risk of laboratory-acquired infections and accidents. This study assessed the knowledge, attitude, and Practice (KAP) of biosafety and related factors among laboratory staff. Methods: A cross-sectional study was conducted among 150 laboratory staff recruited through stratified and random sampling. Data were collected using a validated, self-administered questionnaire that assessed knowledge, attitude, and practice related to biosafety. Descriptive analysis, one-way ANOVA, Pearson correlation and multiple linear regression were performed to examine differences and associations. Results: The findings revealed that laboratory staff demonstrated moderate knowledge (74.8%), good attitudes (95.5%), and good practices (93.7%) towards biosafety. Significant differences in knowledge [F (2,147) = 23.7, p < 0.001] and Practice [F(2,147) = 21.36, p < 0.001] were observed across schools. A moderate positive correlation was found between Practice and knowledge (r = 0.518, p < 0.01) and between Practice and attitude (r = 0.342, p < 0.01). Higher education was associated with better knowledge and attitudes, while longer years of employment were negatively associated with knowledge. No demographic factors significantly predicted practice. Conclusions: Laboratory staff demonstrated positive attitudes and good practices, but showed only moderate knowledge of biosafety. Regular training, continuous education, and standardised biosafety programs are crucial for enhancing knowledge and maintaining safe laboratory practices. This study provides essential evidence to strengthen biosafety training and policies in Malaysian universities, supporting safer laboratory environments and improved institutional preparedness.
Co-Authors Abdul Samad, Nurul Izzah Adinda, Atik Afifah Anwar Akbar, Muhammand Al Hafez Husein Alfajri, Ilham Alfiyyah, Nurul Anastasia, Amanda Puspita Annisa, Sarah Anwar, Afifah Arnanda, Riyansyah Ashari Ashari Astuti, Wistiani Awaldi, Doifi Azizah, Regina Nur Bahzomi Fuadi Chinta Yolanda Sari Dinilhaq, Fauzal Elsusi Martha Elvina, Rodiana Entianopa, Entianopa Erick Irawadi Alwi Febriyanto Karyatibrata Feriani, Pipit Firdaus Yustisia Sembiring Firdaus Yustisia Sembiring fitri, lennisa Fitriyani Umar Gusnawati Sulaeman Hajizah, Wardah Hamdani Hamzah, Nurul Ainun Hariansyah, Fitra Dwi Hatta, Andi Muhammad Iqra Rezky Helen Mefriani Herman Ice Irawati Izma Fahria Kafit, M lilis nurhayati Listiawaty, Renny Lydia Erfina Martha, Elsusi Maryana, Tatik Mega Gemala Mohd Amin, Nursuhaili Mohd Firdaus Yhaya, Mohd Firdaus Muhammad Farhan Nadya Indah Sari Nasir, Haidawati Nik Mohd Razali, Nik Nur Fatin Athirah Novela Sari Novelasari Novendra Hidayat Noviyanti Nur Jading, Rasmin Nurfadilla, Muhammad Izra Nurul, Sarah Fila Oktarizal, Hengky Pramawati, Anita Priyambada, Galih Regina Nur Azizah Roni Saputra Roni Saputra, Roni Sa&#039;diah Erlina Sarbiah, Andi Sari, Nadya Indah Sari, Novela Sembiring, Firdaus Yustisi Sembiring, Firdaus Yustisia Siburian, Roliana Sugianto, Ardi Abimanyu Sugiarto Sugiarto Sukri, Ahmad Sulaeman, Gusnawati Suroso suroso Suroso Suroso Taufik Fitriyanto Taufik Fitriyanto Ulla Amalia, Rizqi Umar, Fitriyani Utami, Fathia Wianida Vika Adasari Yulita Wiwin Trioktoriana Zahara Gema Gatra