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The Analysis of the Chlorogenic Acid in the Ethanol Fraction of Robusta Coffee Beans and Its Effect on Glucose Levels in Wistar Rats Hasanuddin, Rusman; Jasmiadi, Jasmiadi; Abdillah, Nurliana
Disease Prevention and Public Health Journal Vol 15, No 2 (2021): Disease Prevention and Public Health Journal
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/dpphj.v15i2.4705

Background: The metabolic disorder caused by high blood glucose levels and pancreatic beta-cell damage is known as diabetes mellitus. Indonesia itself is the 7th country in the world with the number of people with diabetes mellitus. Indonesia occupies rank 7 in the world with the number of people with diabetes mellitus. Apart from that, robusta coffee (Coffea canephora L) is one of the most popular drinks globally, including Indonesia. Chlorogenic acid in coffee beans effectively reduces cell damage due to free radicals, including minimizing excessive glucose release from the liver into the blood. Objective: This study aimed to analyze the effects of chlorogenic acid in the ethanolic fraction of robusta coffee (Coffea canephora L) beans on blood glucose levels in Wistar rats. Method: The researchers applied an experimental study with a randomized pretest-posttest control group design. The beans of robusta coffee were extracted using the Maceration method and then fractionated using a hexane and ethyl acetate solvent. The concentration of the obtained remaining fraction was measured using a spectrophotometer. Furthermore, hyperglycemia testing included 30 Wistar rats induced with 20% glucose for 3 â€“ 4 weeks. They were then given the ethanol fraction of robusta coffee with a dose of 400 mg/kg BW and 500 mg/kg BW. Meanwhile, metformin served as a positive control, and NaCMC served as a negative control. Results: The chlorogenic acid analysis in the ethanol fraction of robusta coffee on a spectrophotometer with a concentration of 37% indicated a decrease of 16.66% on the negative control and 48.06% on the positive control. Meanwhile, the ethanol fraction of each control was 51.53% and 52.16%, respectively. Conclusion: The ethanol fraction of the robusta coffee significantly affects the decrease in blood glucose levels in Wistar rats.

The Analysis of the Chlorogenic Acid in the Ethanol Fraction of Robusta Coffee Beans and Its Effect on Glucose Levels in Wistar Rats Rusman Hasanuddin; Jasmiadi Jasmiadi; Nurliana Abdillah
Disease Prevention and Public Health Journal Vol. 15 No. 2 (2021): Disease Prevention and Public Health Journal
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/dpphj.v15i2.4705

Background: The metabolic disorder caused by high blood glucose levels and pancreatic beta-cell damage is known as diabetes mellitus. Indonesia itself is the 7th country in the world with the number of people with diabetes mellitus. Indonesia occupies rank 7 in the world with the number of people with diabetes mellitus. Apart from that, robusta coffee (Coffea canephora L) is one of the most popular drinks globally, including Indonesia. Chlorogenic acid in coffee beans effectively reduces cell damage due to free radicals, including minimizing excessive glucose release from the liver into the blood. Objective: This study aimed to analyze the effects of chlorogenic acid in the ethanolic fraction of robusta coffee (Coffea canephora L) beans on blood glucose levels in Wistar rats. Method: The researchers applied an experimental study with a randomized pretest-posttest control group design. The beans of robusta coffee were extracted using the Maceration method and then fractionated using a hexane and ethyl acetate solvent. The concentration of the obtained remaining fraction was measured using a spectrophotometer. Furthermore, hyperglycemia testing included 30 Wistar rats induced with 20% glucose for 3 â€“ 4 weeks. They were then given the ethanol fraction of robusta coffee with a dose of 400 mg/kg BW and 500 mg/kg BW. Meanwhile, metformin served as a positive control, and NaCMC served as a negative control. Results: The chlorogenic acid analysis in the ethanol fraction of robusta coffee on a spectrophotometer with a concentration of 37% indicated a decrease of 16.66% on the negative control and 48.06% on the positive control. Meanwhile, the ethanol fraction of each control was 51.53% and 52.16%, respectively. Conclusion: The ethanol fraction of the robusta coffee significantly affects the decrease in blood glucose levels in Wistar rats.

Formulation and Activity Test of Repellent Spray Marigold Flower Essential Oil (Tagetes erecta L.) in Aedes aegypti Mosquitoes Nur Alfiah Irfayanti; Jasmiadi Jasmiadi; Alda Ta.ri
Journal Syifa Sciences and Clinical Research Vol 4, No 2 (2022): Volume 4 Edisi 2 2022
Publisher : State University of Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/jsscr.v4i2.14161

The purpose of this study was to determine the activity of spray preparations of marigold flower essential oil (Tagetes erecta L.) on the Aedes aegypti mosquito. Marigold flower essential oil using water distillation method using aquadest and rabbits as test animals. The study was conducted by taking five treatments, namely one negative control group K(-), three treatment groups with variations in concentration (P1) 2.5%, (P2) 5%, P(3) 10% and one positive control group K( +). The results showed that repellent spray had repulsion, control (-) was 50.67%, 2.5% concentration was 68.33%, 5% concentration was 78.67%, 10% concentration was 96% and control (+ ) by 100%. The results of the protection test showed that the repellent spray containing marigold flower essential oil had the highest activity at a concentration of 10%. Based on the results of statistical analysis showed that the calculated f is greater than f table 5% and 1% and the results of the follow-up test of SNK (Newman Keuls) showed that the concentration of 10% was not significantly different from the control (+).

Phytochemical Screening, Relationship of Total Phenolic with Antioxidant Activity Of Ethanol and Methanol Extracts of Kesambi (Schleichera oleosa (Lour.) Oken) Bark Nur Alim; Tahirah Hasan; Rusman Rusman; Jasmiadi Jasmiadi; Zulfitri Zulfitri
Jurnal Ilmiah Sains Volume 22 Nomor 2, Oktober 2022
Publisher : Sam Ratulangi University

Phenolic content has a role in antioxidant activity. The higher the phenolic content of a test sample, the higher the antioxidant activity, which is indicated by a smaller IC50 value. Methanol and 70% ethanol are solvents that have been proven to be widely used to extract total plant phenolic compounds. This study aims to determine the phytochemical content and the relationship of total phenolic content with antioxidant activity of ethanol and methanol extract of Schleichera oleosa (Lour.) Oken) bark using UV-Vis spectrophotometry. Schleichera oleosa (Lour.) Oken was extracted by maceration using 70% ethanol and methanol as solvent. Qualitative analysis of phytochemical compounds using specific reagents, analysis of total phenolic content, and antioxidant activity test using the DPPH method using UV-Vis spectrophotometry at 764 nm and 515 nm, respectively. The results of the qualitative test showed that the ethanol and the methanol extract was positive for alkaloids, flavonoids, and phenolics. The results of the analysis of total phenolic content and antioxidant activity showed that the 70% ethanol extract had a total phenolic content of 7.6829 mg gallic acid equivalent/g with IC50 28.5240 ppm and the methanol extract had a total phenolic content ofÂ 9.2057 mg gallic acid equivalent/g with IC50 1.6191 ppm. From these results, it was concluded that the higher the total phenolic content, the higher the antioxidant activity.Keywords: Antioxidant; Schlechera oleosa (Lour.) Oken.; total phenolicÂ Skrining Fitokimia dan Hubungan Kadar Fenolik Total dengan Aktivitas Antioksidan Ekstrak Etanol dan Metanol Kulit Batang Kesambi (Schleichera oleosa (Lour.) Oken)ABSTRAKKadar fenolik memiliki peran terhadap aktivitas antioksidan. Semakin tinggi kadar fenolik suatu sampel uji maka aktivitas antioksidannya juga semakin tinggi, yang ditunjukkan dengan nilai IC50 yang lebih kecil. Pelarut metanol dan etanol 70% merupakan pelarut yang telah terbukti banyak digunakan untuk menarik senyawa fenolik total tanaman.Â Penelitian ini bertujuan untuk menentukan kandungan fitokimia, hubungan kadar fenolik total dengan aktivitas antioksidan ekstrak etanol dan ekstrak metanol kulit batang kesambi (Schleichera oleosa (Lour.) Oken) menggunakan spektrofotometri UV-Vis. Kulit batang kesambi (Schleichera oleosa (Lour.) Oken) diekstraksi dengan cara maserasi menggunakan pelarut etanol 70% dan pelarut metanol. Analisis kualitatif senyawa fitokimia menggunakan pereaksi spesifik, analisis kadar fenolik total dan uji aktivitas antioksidan dengan metode DPPH menggunakan spektrofotometri UV-Vis masing-masing pada panjang gelombang 764 nm dan 515 nm. Hasil uji kualitatif menunjukkan bahwa ekstrak etanol dan ekstrak metanol kulit batang kesambi (Schleichera oleosa (Lour.) Oken) positif mengandung alkaloid, flavonoid dan fenolik. Hasil analisis kadar fenolik total dan uji aktivitas antioksidan menunjukkan bahwa ekstrak etanol 70% memiliki kadar fenolik total sebesar 7,6829 Â mgGAE/g ekstrak dengan nilai IC50 sebesar 28,5240 ppm dan esktrak metanol memiliki kadar fenolik total sebesar 9,2057 mgGAE/g ekstrak dengan nilai IC50 sebesar 1,6191 ppm. Dari hal tersebut disimpulkan bahwa semakin tinggi kadar fenolik total maka semakin tinggi pula aktivitas antioksidannya.Kata kunci: Antioksidan; fenolik total; Schlechera oleosa (Lour.) Oken

UJI TOKSISITAS AKUT EKSTRAK ETANOL KLIKA KAMANDRAH (CROTON TIGLIUM L.) TERHADAP LARVA ARTEMIA SALINA LEACH DENGAN METODE BRINE SHRIMP LETHALITY TEST Rusman; A. Suparlan Isya Syamsu; Jasmiadi; Sri Wahyuni Gaffar
Journal Prima Health Science Vol 1 No 3 (2020): Mei
Publisher : LPPM STIKes Prima Indonesia

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Research on the acute toxicity test on an ethanol extract of camandrah clika (Croton tiglium L.) ethanol extract onArtemia salina Leach larvae was conducted. With the Brine Shrimp Lethality Test method. The aim of this researchwas to determine the acute toxicity potential of the camandrah clika ethanol extract that extracted by macerationwith 96% ethanol solvent, then evaporated to produce a thick extract, then it was tested against Artemia salina Leachlarvae at concentrations of 1000 ppm, 100 ppm, ten ppm, one ppm and negative control of seawater. The resultsobtained were based on an analysis of 3,706/mL, classified as highly toxic to the level of Artemia salina Leach.

Antipyretic Activity of Ethanol Extract from Beligo (Benincasa hispida (Thunb.) Cogn.) Fruit Flesh in Wistar Albino Rats (Rattus norvegicus) Nur Alim; Rusman Hasanuddin; Jasmiadi Jasmiadi; Musdalifah Musdalifah; A. Ihdinal Hagg; Nur Linda
Jurnal Ilmiah Sains Volume 23 Nomor 2, Oktober 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jis.v23i2.48443

The people of South Sulawesi employ beligo as a plant with fever-reducing properties. The utilized part is the flesh of its fruit. The beligo fruit contains fatty acids, flavonoids, phenolics, and steroids that hold promise as antipyretic agents. The study aimed to determine the antipyretic activity of ethanolic extract from beligo fruit flesh on male Wistar rats induced with peptone solution. The research method encompassed maceration extraction using 70% ethanol solvent and antipyretic activity testing using 15 male Wistar rats divided into five treatment groups. Group I served as the negative control and received SODIUM-CMC 1%, groups II, III, and IV received ethanolic extract of beligo fruit flesh at doses of 250 mg/kg BW, 500 mg/kg BW, and 750 mg/kg BW respectively, and group V, the positive control, received paracetamol tablets. Measurements of initial temperature, temperature after induction, and temperature after treatment were taken at 30-minute intervals for 180 minutes. Data were statistically analyzed using a Completely Randomized Design (CRD) and Duncan test. The research findings revealed a fever reduction percentage of 1,57% for group I, 4,03% for group II, 4,28% for group III, 4,45% for group IV, and 5,25% for group V. Data analysis indicated that ethanolic extract of beligo fruit flesh at doses of 250 mg/kg BW, 500 mg/kg BW, and 750 mg/kg BW exhibited significant antipyretic activity (p < 0.05). The ethanolic extract from beligo fruit flesh possesses antipyretic activity in induced Wistar rats, suggesting its potential use as a fever-reducing medication. Keywords: Antipyretic; Benincasa hispida (Thunb.) Cogn; fever

UJI KESERAGAMAN BOBOT OBAT RACIKAN DALAM BENTUK SEDIAAN KAPSUL GELATIN KERAS DARI BEBERAPA APOTEK DI MAKASSAR Jasmiadi
Journal of Pharmaceutical Science and Herbal Technology Vol. 3 No. 1 (2018): Journal of Pharmaceutical Science and Herbal Technology
Publisher : STIKES Nani Hasanuddin Makassar

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Obat Racikan merupakan obat yang dibentuk dengan mencampur bahan-bahan aktif yang kemudian dimasukkan ke dalam cangkang kapsul. Dalam hal ini kapsul merupakan sediaan padat yang terdiri dari satu atau lebih bahan padat dengan atau tanpa bahan inert yang dimasukkan dalam cangkang yang umumnya dibuat dari gelatin yang sesuai. Kapsul gelatin keras yang memiliki keuntungan yakni mencegah bau dan rasa pahit pada obat sehingga sangat popular dikalangan masyarakat. Oleh karena itu, untuk memperoleh mutu obat racikan kapsul harus memenuhi persyaratan yang telah ditentukan salah satunya adalah uji keseragaman bobot. Telah dilakukan penelitian dengan tujuan untuk mengetahui keseragaman bobot obat racikan kapsul gelatin keras. Hasil penelitian menunjukkan bahwa bobot dari sampel apotek diperoleh pada apotek A terdapat 10 kapsul yang menyimpang dari persyaratan bobot dengan persentase penyimpangan tertinggi 17%, penyimpangan sedang 12% dan terendah 7,97% sedangkan untuk apotek B, C, D dan E tidak satu pun kapsul yang menyimpang dari persyaratan bobot. Berdasarkan penelitian dapat disimpulkan bahwa keseragaman bobot pada apotek A tidak memenuhi persyaratan bobot kapsul sedangkan apotek B,C,D dan E telah memenuhi persyaratan bobot kapsul. Diharapkan kepada peneliti selanjutnya untuk melakukan penelitian pada uji disolusi, keseragaman kandungan dan waktu hancur pada obat racikan kapsul Keras atau Lunak.

Aphrodisiac Activity of Beligo (Benincasa hispida (Thunb.) Cogn.) Seeds of Ethanol Extract in Mice Alim, Nur; Jasmiadi, Jasmiadi; Nadillah, Nadia; Indrawaty Kadir , Ira; Afirah, Nur
Jurnal Kedokteran Brawijaya Vol. 33 No. 2 (2024)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2024.033.02.1

Beligo (Benincasa hispida (Thunb.) Cogn.), is empirically used by the people of South Sulawesi-Indonesia as an aphrodisiac. Beligo seeds contain alkaloids, phenolics, flavonoids, and unsaturated fatty acids, which have the potential to act as aphrodisiacs. The primary objective of this research is to determine the aphrodisiac activity of an ethanol extract from beligo seeds in mice. The procedure entails extraction and aphrodisiac evaluation using introduction, climbing, and coitus test parameters. Aphrodisiac testing was carried out for seven days using 25 mice as experimental animals, divided into five groups. Each group consists of four males and one female. Group I was given Sod. CMC 1% as a negative control; II, III, and IV were given ethanol extracts of beligo seeds at doses of 350mg/kg, 700mg/kg, 1400mg/kg BW, and group V, as a positive control, was given Yohimbine. The results showed that the ethanol extract of beligo seeds at doses of 350mg/kg BW, 700mg/kg BW, and 1400mg/kg BW had aphrodisiac activity (p=0.0001) compared to Sod. CMC 1% based on data on the amount of introduction and climbing. However, based on data on the number of coitus, it showed that the ethanol extract of beligo seeds at doses of 350mg/kg BW (p=0.072) had no aphrodisiac activity, 700mg/kg BW (p=0.007), 1400mg/kg BW (p=0.0001) had aphrodisiac activity compared to Na-CMC 1% and only a dose of 1400 mg/kg BW had the same aphrodisiac activity compared with Yohimbine (p=0.967). It is concluded that the ethanol extract of beligo seeds had aphrodisiac activity.

Aktivitas Antibakteri Ekstrak Etanol Daging Buah Beligo (Benincasa hispida (Thunb.) Cogn.) terhadap Escherichia coli dan Staphylococcus aureus Hasanuddin, Rusman; Alim, Nur; Jasmiadi; Fauzan, Ahmad
Jurnal Novem Medika Farmasi Vol. 1 No. 3: Volume 1 Issue 3
Publisher : Program Studi Farmasi, Universitas Islam Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59638/junomefar.v1i3.527

Antibacterial is a compound that can be used to inhibit or kill bacteria. Ethanol extract of Beligo fruit flesh [Benincasa hispida (Thunb.) Cogn.)] contains flavanoid compounds that can be used as antibacterials. The purpose of this research was to determine the antibacterial activity of the ethanol extract of beligo fruit flesh [Benincasa hispida (Thunb.) Cogn.)] against bacteria Escherichia coli and Staphylococcus aureus. Research methods include macerated extraction using 70% ethanol and testing antibacterial activity against bacteria Escherichia coli and Staphylococcus aureus with diffusion methods using disc paper. The result obtained using concentration of 12,8%; 25,6%; 51,2% each had an inhibitory diamter of 7,34 mm; 8,97 mm; 12,12 mm for Staphyloccous aureus bacteria while the inhibitory diamter is 6,45 mm; 7,33 mm; 9,91 mm for Escherichia coli bacteria. The conclusion of this research is that the ethanol extract of Beligo fruit flesh has antibacterial activity against Escherichia coli and Staphylococcus aureus.

Aktivitas Antibakteri Ekstrak Etanol Klika Jambu Bol (Syzygium malaccense (L.) Merr. & Perry) terhadap Bakteri Shigella dysenteriae Jasmiadi, Jasmiadi; sangka pratama, agus; Musdalifah, Musdalifah; aprianti, ayu
Jurnal Novem Medika Farmasi Vol. 1 No. 3: Volume 1 Issue 3
Publisher : Program Studi Farmasi, Universitas Islam Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59638/junomefar.v1i3.540

Penelitian tentang aktivitas antibakteri ekstrak etanol klika jambu bol telah dilakukan. Tujuan penelitian ini adalah untuk mengetahui apakah ekstrak etanol klika jambu bol memiliki daya hambat terhadap Bakteri Shigella dysenteriae. Metode penelitian meliputi ekstraksi secara maserasi menggunakan etanol 96% dan pengujian aktivitas antibakteri terhadap bakteri Shigella dysenteriae dengan metode difusi agar menggunakan kertas cakram. Konsentrasi ekstrak jambu bol yang digunakan, 6.25%,12.5%, 25%, dan masing-masing memiliki parameter zona hambat sebesar 7,96 mm; 8,93 mm; 10,38 mm. Hasil penelitian menunjukkan bahwa ekstrak jambu bol mempunyai aktivitas terhadap bakteri Shigella dysenteriae.

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