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Journal : Veterinary Biomedical and Clinical Journal

The Method of Total Erythrocyte and Leukocyte Counting in Raptors at Wildlife Rescue Centre (WRC) Jogja Hardian, Andreas Bandang; Nugrahani, Warih Pulung Nugrahani; Rahmawati, Irhamna Putri Rahmawati; Megarani, Dorothea Vera Megarani
Veterinary Biomedical and Clinical Journal Vol. 2 No. 2 (2020)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2020.002.02.2

Abstract

Most of raptor species rehabilitated for conservation purpose at WRC Jogja are eagles. Annually, we perform general raptor examination involving conventional routine hematologic examination. However, low sample quality and over dense blood cell population during total erythrocyte and leukocyte counting were deemed as obstacles. This study aimed to give wider insight in how total erythrocyte and leukocyte counting was performed at WRC Jogja to overcome those obstacles. Firstly, handling and restraint were done physically adapting the Aba method. One-to-two half millilitres of blood was collected from the ulnar vein using 3-ml-syringe with 24-gauge-needle which was then preserved in an EDTA-containing tube and proceeded to subsequent method no more than 3 hours after collection. No hypovolemic shock was observed. During total erythrocyte and leukocyte counting using Neubauer-ruled counting chamber, we modified the dilution factor to be 250-fold and 50-fold respectively. This method yielded a countable cell density and can be applied in other species in similar condition.
Analysis of Slaughtering Points Distribution during Eid al-Adha 1440H in Malang City, East Java, Indonesia Adrenalin, Sruti Listra; Airlangga, Gegana Wimaldy; Hardian, Andreas Bandang
Veterinary Biomedical and Clinical Journal Vol. 2 No. 2 (2020)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2020.002.02.5

Abstract

Eid al-Adha is an annual Muslim celebration throughout the world indicated by sacrificing animals such as cattles, goats or sheep. This study aimed to analyse the distribution of slaughtering points, types and the number of animals being slaughtered as well as antemortem and postmortem abnormalities of the carcasses during Eid al-Adha 1440 H in Malang City. Data were collected from the geocode service location and the secondary data were obtained through questionnaire. This study listed a total of 145 slaughtering points located in Blimbing, Kedung Kandang, Klojen, Lowokwaru, and Sukun District with 3102 animals being sacrificed. The slaughtering points were taken place in the office yards, mosque yards, home yards, school yards or other public fields. As many as 75.86% of slaughtering points provided special shelters for the animals before being slaughtered. The antemortem examination resulted that animals from 21.38% of the slaughtering points suffered from conjunctivitis, lesions on the skin, broken horns, and lameness. Proportions of apparent postmortem abnormalities were measured respectively: cattle 64.83%, goat 41.38%, and sheep 4.14%.  Hence, we recommend to further involve the veterinarians to monitor the animal condition during Eid al-Adha and to ensure the slaughtering processes were done properly.
Yolk Binding Treatment for Reticulated Python in House of Pet Clinic: Penanganan Kasus Yolk Binding Pada Ular Sanca batik (Python reticulatus) di Klinik House of Pet Isnaeni, Intan Kirana; Setianingrum, Ani; Hardian, Andreas Bandang; Yesica, Reza; Herlambang, Anugrah Niki
Veterinary Biomedical and Clinical Journal Vol. 4 No. 1 (2022)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2022.004.01.6

Abstract

Yolk binding is a condition where the egg yolk as food reserves while the snake still in the egg phase is notcompletely absorbed in the snake’s body when the snake has become neonatal. This study aims todetermine the handling of cases of yolk binding in Python reticulatus. A 3-month-old female Pythonreticulatus was brought by the owner to the vet with complaints of not defecating after ten days from thelast feeding on and the presence of a ¼ lump from the caudal. The results of the stool examinationshowed the number of protozoa. The x-ray results, show the air-filled medial with radiolucent colorintensity and a mass with radioopaque color intensity. Based on the signals, history, physical examination,and investigations, a diagnosis of yolk binding was obtained. The treatment given is surgery, drug therapy,and post-operative care. After being brought home, five days after surgery snake died.
Effects of Lycopene Treatments on Development, Hatchability, and Heart Rate of Zebrafish Embryos under Heat Stress Exposure Tuska, Habib Syaiful Arif; Salsabila, Odifiannisa Ayu; Lonameo, Bonick Kartini; Hardian, Andreas Bandang; Lesmana, M. Arfan; Yesica, Reza
Veterinary Biomedical and Clinical Journal Vol. 6 No. 1 (2024)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2024.006.01.1

Abstract

Lycopene, a potent antioxidant predominantly found in red-hued fruits and vegetables such as tomatoes, has been incorporated into embryonic maturation media in vitro, demonstrating enhancements in embryo quality. Despite these advancements, the specific effects of lycopene on Zebrafish embryo quality remain unexplored. This study aims to investigate the impact of lycopene (0.65 ppm) on the development of Zebrafish embryos, focusing on hatchability (HA), and heart rate (HR) under conditions of heat stress (HS). Fertile Zebrafish embryos at the gastrula stage were induced HS (30oC; 1 hour). The embryos will be segregated into two groups: those exposed to HS and nonHS (28oC). Each group will receive one of three treatments (four replicates): control / without lycopene (P1), 2μL lycopene (P2), and 4μL lycopene (P3). Observations using an inverted microscope was done every three up to 48 hours post-fertilization (hpf), and subsequently every five hours up to 96 hpf. Quantitative data obtained for HA and HR were analyzed with SPSS, One-Way ANNOVA (p<0.05). The antioxidants contained in lycopene can influence the speed and success of Zebrafish embryo development and HA exposed to HS. In addition, the antioxidant in lycopene can also suppress the HR frequency of Zebrafish embryos exposed to HS. However, more studies about the optimum dose, purity, and stability of the lycopene extract should be done to make a definitive conclusion.