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Coaching clinic research proposal writing: A practical guide to your research success H. Hardani; Sri Sahbany; Samsul Hadi; Atri Sri Ulandari; Ni Nyoman Ariwidiani; Agus Supinganto
Journal of Community Service and Empowerment Vol. 5 No. 2 (2024): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jcse.v5i2.32409

Abstract

Writing a research proposal is a crucial first step in determining the success of your research project. However, authors often face challenges in formulating proposals that are clear, relevant, and persuasive. The purpose of this service is to help researchers, especially young researchers in making research proposals. The method of implementing this service uses a Coaching Clinic, discussion, and mentoring which is carried out in a hybrid manner with pre-test and post-test.  Participants in this Coaching Clinic were young lecturers from Politeknik Medica Farma Husada Mataram and several other universities who participated online. There were 20 participants and it was held online through a Zoom meeting on December 16, 2023. The main target of this activity is young lecturers who have minimal experience writing research proposals. The results of this service show an increase in lecturers' abilities or skills and understanding in writing research proposals by 84.50%. Coaching clinics can improve the ability of lecturers to write research proposals.
Identification of Lactic Acid Bacteria From Pineapple Peels Fermented Beverages Through 16S rRNA Gene Analysis Ariwidiani, Ni Nyoman Nyoman; Pauzan, Pauzan; Wahdaniyah, Rabiyatun; Mahavira Diputra, I Ketut
Jurnal Sain Veteriner Vol 44, No 1 (2026): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.112560

Abstract

Stunting is associated with intestinal microbiota dysbiosis, which disrupts bodily functions and contributes to stunted growth in children. Consuming probiotics is an alternative way to prevent gastrointestinal infections caused by dysbiotic microbiota. Probiotics work by balancing the composition of the intestinal microbiota and stimulating the immune response. The use of fermented products is well known; one example is tepache, a fermented pineapple peel drink containing lactic acid bacteria with potential as a probiotic. This study aims to identify lactic acid bacteria from fermented pineapple peel drinks with potential as probiotics for intestinal microbiota health. Pineapple peel fermentation was carried out for 3 days; the resulting fermentation liquid was cultured on MRS agar and tested for characteristics using biochemical tests. Identification of isolates from the pineapple peel fermentation culture was performed by sequencing the 16S rRNA gene. The results of the isolate culture showed growth of 2 bacterial isolates with characteristics consistent with LAB colonies. Microscopic tests showed that isolate N1 was a gram-positive, rod-shaped LAB, and isolate N2 was a gram-positive, cocobacilli-shaped LAB. Identification of the 16S rRNA gene from isolate N1 showed characteristics similar to Lactiplantibacillus plantarum, while isolate N2 showed characteristics similar to Leuconostoc mesenteroides. These isolates are common lactic acid bacteria found in fermented foods and may be potential probiotic candidates, but functional and safety evaluations are needed to confirm their probiotic properties.
Identification of Bacteria in Foot Wound Swabs from Patients Diabetes Mellitus Nurul Humairoh; Ni Nyoman Ariwidiani; Baiq Isti Hijriani
Laboratory Journal of Infectious Diseases Vol. 1 No. 2 (2025): Laboratory Journal of Infectious Diseases
Publisher : Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Diabetes mellitus is a chronic metabolic disorder characterized by persistent hyperglycemia that predisposes patients to impaired wound healing and chronic infections, including diabetic foot ulcers. Bacterial colonization of these wounds plays a critical role in delayed healing, increased morbidity, and risk of amputation. This study aimed to identify the bacterial profiles isolated from foot wound swabs of patients with diabetes mellitus. A descriptive observational study was conducted using foot wound swab samples collected from patients with diabetes mellitus receiving wound care at Mandalika Care, Mataram City. Bacterial identification was performed through macroscopic and microscopic examination, Gram staining, and standard biochemical tests following culture on Blood Agar Plate (BAP) and MacConkey Agar (MCA). Data were analyzed descriptively and presented as frequencies and percentages. Six samples met the inclusion criteria and were analyzed. All isolates were Gram-negative bacteria. The bacterial species identified were Pseudomonas aeruginosa (33,36%), Morganella morganii (16,6%), Pseudomonas putrefaciens (16,6%), Enterobacter agglomerans (16,6%), and Pseudomonas mallei (16,6%). The conclusion is that Gram-negative bacteria predominated in diabetic foot wound infections in this study, with Pseudomonas aeruginosa being the most frequently isolated species. These findings highlight the importance of early microbiological examination to guide appropriate wound management and antimicrobial therapy in diabetic patients.