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All Journal Buletin Peternakan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences JURNAL PETERNAKAN Dinamisia: Jurnal Pengabdian Kepada Masyarakat Jurnal Peternakan (Jurnal of Animal Science) Lumbung: Jurnal Ilmiah Politeknik Pertanian Negeri Payakumbuh Jurnal Nutrisi Ternak Tropis dan Ilmu Pakan Budimas : Jurnal Pengabdian Masyarakat Jurnal Teknik Industri Terintegrasi (JUTIN) Ovozoa: Journal of Animal Reproduction Jurnal Triton Jurnal Ilmiah AgriSains Livestock and Animal Research Jurnal Inspirasi Peternakan
Rodiallah, Muhamad
Faculty Of Agriculture And Animal Science, Universitas Islam Negeri Sultan Syarif Kasim Riau, Pekanbaru, 28293
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Energy Engineering Environmental Science Industrial & Manufacturing Engineering Mechanical Engineering Social Sciences Veterinary Other

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The Effectiveness of Adding Different Sucrose in Tris-Egg Yolk Diluent on the Sperm Fertility of Sexing Swamp Buffalo (Bubalus bubalis): Adding Different Sucrose in Tris-Egg Yolk Diluent on the Sperm Fertility of Sexing Swamp Buffalo Yendraliza Yendraliza; Allif Bukhori; Edi Erwan; Ria Sari Gail Sianturi; Diana Andrianita Kusumaningrum; Muhamad Rodiallah; Zumarni
Journal of Tropical Life Science Vol. 13 No. 2 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.02.17

Abstract

Spermatozoa sexing requires a diluent that can protect and provide an optimal environment to maintain the quality of spermatozoa. This study targets to determine the effectiveness of adding sucrose to tris-egg yolk diluent on the sperm quality of the swamp buffaloes produced by sexing with the egg albumin method. Semen was collected once a week for 12 consecutive weeks with an artificial vagina from three male buffaloes at the Indonesian Center for Animal Research and Development (ICARD) in April - June 2021. Semen was separated by sedimentation using 10% and 30% egg albumins. Semen from sexing was divided into four diluent treatments, namely 20% tris-egg yolk (TEY), TEY+0% sucrose, TEY+0.2% sucrose, TEY+0.3% sucrose, and TEY+0.4% sucrose. Parameters measured included: motility, viability, intact plasm membrane, and intact acrosome cap of buffalo sperm. The results showed the motility of X sperm: 43.75-47.5%, Y sperm: 40-45%, the viability of X sperm: 78.25-79.75% and Y sperm: 77.25-79.75%, intact plasm membrane of X sperm: 70.5-71.5% and Y sperm: 70.5-71%, and viable acrosome-intact for X sperm: 79-80% and Y sperm: 78.75-79.5%. The addition of sucrose up to 0.4% into tris-egg yolk diluent was proven can maintain motility, viability, and integrity of cell membrane and acrosome of spermatozoa of water buffalo that was sexed with egg albumin.
Co-Authors Allif Bukhori Anwar Efendi Harahap Anwar Harahap Arbiansyah Arbiansyah, Arbiansyah Arsyadi Ali Bakhendri Solfan Bayu Nuari Ramadhan Dewi Ananda Mucra Dewi Ananda Mucra Diana Andrianita Kusumaningrum Edi Erwan Edi Erwan Effendi, Anwar Elfi Rahmadani Elviriadi, Elviriadi Eniza Saleh Ernawan, Wahyu Evi Irawati Fitra, Deni Hafizon, M. Irawati, Evi Jati, Putri Jati, Putri Zulia Juliantoni, Jepri Jully Handoko Khairuddin Kurniawan, Isal Muhammad Ridho Mulia, Fungki Firma Mulianti, Mestia Nauli, Sarah Noviana, Riskia Pamungkas, Yusuf Tri Rahmad Yusuf Rahmi Febriyanti Rahmi Febriyanti Ramdani, Dedi Restu Misrianti Ria Sari Gail Sianturi Sadarman Saleh, Eniza Triani Adelina Umar Faruq Umul Habiyah, Umul Yendraliza - Zumarni Zumarni Zumarni Zumarni Zumarni, Zumarni