Claim Missing Document
Check
Articles

The Effectiveness Test of Betel Leaf Ethanol Extract Cream (Piper Betle Linn) Toward Propionibacterium acnes Bacterial Growth Resva Meinisasti; Zamharira Muslim; Krisyanella; Raden Sunita
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 4 No. 2 (2020): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v4i2.112

Abstract

Background: One of skin diseases that is frequently taking attention among teenagers and young adults is acne or in medical term called acne vulgaris. Acne treatment can be treated by repairing follicular abnormalities, decreasing sebum production, decreasing the number of Propionibacterium acnes colonies and reducing inflammation of the skin. The bacterial population of Propionibacterium acnes can be reduced by giving an antibacterial substance such as erythromycin, clindamycin and benzoyl peroxide. In the treatment of acne we can get antibacterial originating from nature, one of which can be obtained in the secondary metabolism of plants. The extracts and essential oils of betel leaf contain antibacterial and antifungal activities. The effectiveness of the use of betel leaf ethanol extract (Piper betle Linn) in acne treatment can be improved by creating formulations in form of cream preparations. Formulations in cream preparations will affect the amount and speed of active substances that can be absorbed. Objectives: To determine the effectiveness of the ethanol extract cream of betel leaf (piper betle linn) cream in formulas with what percentage of active substance has the most-inhibitory effect on the growth of Propionibacterium acnes. Methods:The study used experimental research. Propionibacterium acnes samples were diluted in 0.9% physiological NaCl sterile and embedded in Nutrient Agar (NA) media. The media was inserted into an incubator at 37 ° C for 24 hours. The test of antibacterial activity used the disk diffusion method. The antibacterial activity test results were statistically analyzed using the Statistical Product Services Solution (SPSS 17) program with a confidence level of 95% (α = 0.05). Results: The three creams containing betel leaf ethanol extracts at percentages of 5%, 10% and 15% for each had inhibitory zones: 9.8 mm, 15.85 mm, 17.35 mm. Conclusion: Cream that contains 15% active substance has the strongest inhibition.
Uji Aktivitas Antimikroba Ekstrak Metanol Daun Tabat Barito ( Ficus deltoidea Jack) Krisyanella Krisyanella; Meri Ardianti; Dachriyanus Dachriyanus
Jurnal Farmasi Higea Vol 1, No 1 (2009)
Publisher : STIFARM Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.252 KB) | DOI: 10.52689/higea.v1i1.6

Abstract

An antimicrobial activity studies from methanol extract of the leaves Tabat Barito (Ficus deltoidea Jack) to some bacterias and fungis with diffusion method and determination of Minimum Inhibitory Consentration (MIC) by using dilution method have been done. From antimicrobial activity test by using diffusion method show that methanol extract of the leaves Tabat Barito (Ficus deltoidea Jack) can inhibit the growth of bacterias and fungis. The MIC for staphylococcus aureus is 1%, for staphylococcus epidermis is 0.7%, for staphylococcus aeruginosa is 0.8%, and for eschericha coli is 0.7%.
Produksi Bioplastik Poli (3-Hidroksibutirat) (P(3HB)) Secara Proses Fermentasi Menggunakan Bakteri Bacillus brevis FAAC-20801 Dari Minyak Kelapa Sawit Sebagai Sumber Karbon Krisyanella Krisyanella; Mimi Susanti; Akmal Djamaan
Jurnal Farmasi Higea Vol 2, No 1 (2010)
Publisher : STIFARM Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.223 KB) | DOI: 10.52689/higea.v2i1.20

Abstract

The production of bioplastic P(3HB) with fermentation process by Bacillus brevis FAAC-20801 from palm oil as carbon has been carried out. The fermentation process was conducted throug at pH 7, 37oC and agitation rate of 200 rpm for 60 hours. The characterization of the bioplastic production was based on cell growth, P(3HB) content and P(3HB) percentage. Results showed that the optimum time was 48 hours, with fermentation biomassa 277 mg, P(3HB) content of 7.2 mg/ 20 mg biomassa (36 % b/b). Fermentation process in bioreactor produced P(3HB) higher than in rotary shaker incubator.
Screening Fitokimia Dan Penetapan Potensi Madu Hutan Sebagai Agen Antibakteri Terhadap Bakteri Propinibacterium Acne dan Staphylococcus Aureus Krisyanella Krisyanella; Zamharira Muslim; Resva Meinisasti; Putra Adi Irawan
Jurnal Farmasi Higea Vol 13, No 1 (2021)
Publisher : STIFARM Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52689/higea.v13i1.327

Abstract

Madu dapat membantu menekan pertumbuhan bakteri tertentu melalui beberapa mekanisme salah satunya yaitu dari komposisi kandungan senyawa kimia yang berbeda-beda berdasarkan sumber pakan nektarnya. Perbedaan tersebut diduga mempengaruhi perbedaan aktivitas madu sebagai antibakteri. Jerawat adalah peradangan yang terjadi pada kulit akibat adanya infeksi bakteri pada kelenjar minyak yang tersumbat. Bakteri yang umum menginfeksi jerawat adalah  Staphylococcus aureus dan  Propinibacterium acne. Tujuan penelitian adalah untuk mengetahui kandungan fitokimia dan uji aktivitas antibakteri madu hutan asli Bengkulu terhadap bakteri P.acne dan S. Aureus. Desain penelitian yang digunakan dalam penelitian ini adalah eksperimental laboratorium. Pertama ditetapkan dulu kandungan fitokimia sampel madu, kemudian dilanjutkan dengan penetapan aktvitas antibakteri madu hutan terhadap bakteri P. acne dan S. aureus, potensi ini dilihat dari besarnya nilai Konsentrasi Hambat Minimum (KHM). Sampel madu hutan memiliki kandungan metabolit sekunder. Madu A mengandung Alkaloid dan Terpenoid, Madu B mengandung terpenoid, Madu C mengandung Alkaloid, Madu D mengandung Flavonoid dan alkaloid, sementara Madu E dan F mengandung flavonoid. Dari hasil pengujian aktivitas antibakteri sampel madu hutan memiliki aktivitas antibakteri terhadap bakteri S. aureus  dan P.acne, namun efek aktibakteri paling baik terhadap bakteri S.aureus, dimana pada konsentrasi terkecil, madu masih memberikan daya hambat pada bakteri S.aureus. Madu hutan mengandung metabolit sekunder dan  lebih berpotensi sebagai agen antibakteri terhadap bakteri S. aureus  dibandingkan dengan pada bakteri P. acne
Optimasi Proses Produksi Bioplastik Poli (3-Hidroksibutirat) Dengan Bakteri Bacillus sp FAAC 20801 Menggunakan Bahan Dasar Jerami Padi Setelah Fermentasi Akmal Djamaan; Witra Aulia; Krisyanella Krisyanella
Jurnal Farmasi Higea Vol 3, No 2 (2011)
Publisher : STIFARM Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (504.921 KB) | DOI: 10.52689/higea.v3i2.47

Abstract

The optimation of production process of  bioplastic poly (3 - hidroxybutirat) (P(3HB) fermentation by Bacillus sp FAAC 20801 bacteria, using straw as carbon source has been carried out. Detection of P(3HB) produced using gas chromatography. The highest glucosa level obtain by using H2SO4 5% for hidrolisis. From 46800 mg straw (glucosa concentration 200 mg/mL) produce 5379 mg biomassa and 0.058 mg P(3HB) ; from 83400 mg straw (glucosa concentration 400 mg/mL) produce 5011 mg biomassa and 0.124 mg P(3HB) ; from 142800 mg straw (glucosa concentration 600 mg/mL) produce 3992 mg biomassa and 2.309 mg P(3HB).
Pemanfaatan Jerami Sisa Produk Pertanian sebagai Bahan Dasar Produksi Etanol Secara Fermentasi (Optimasi Produksi Berdasarkan Perbedaan Konsentrasi Inokulum dan Lama Fermentasi) Akmal Djamaan; Mahrani Napitupulu; Krisyanella Krisyanella
Jurnal Farmasi Higea Vol 1, No 2 (2009)
Publisher : STIFARM Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (183.337 KB) | DOI: 10.52689/higea.v1i2.11

Abstract

Research about exploiting of straw as component of ethanol as fermentation based on difference able of inoculums consentration and fermentation time has been studied. Straw utilized in this research is result of agriculture raffle from rice plant type IR 46, which taken away from rice field the district of Lubuk Minturun, Padang. The first, stage hydrolysis was done to straw using H2SO4 P 1% with various time 30, 60, 120, 180 and 240 minute and determined glucose produced for each sample. The fermentation of ethanol by using Saccharomyces cereviceae bacterium were conducted at temperature 30ºC with speed 100 rpm. Sampling was taking every 6 hours (6, 12, 18, 24, 30, 36, 42, 48, 54, 60, 66). The inoculum’s grade level is variation by 1, 2, 3% v/v. Results showed that the optimum hydrolysis time wich mark by highest glucose rate is minute 240 with grade level 0.7765 %. The highest ethanol grade of which can be yielded with inoculum 1% was 0.02066 % v/v at yeast inoculum 2 % was of 0.02549 % v/v and yeast inoculum 3 % was of  0. 07804 % v/v.
Pengaruh Perbandingan Pelarut Ekstraksi Etanol-Air Terhadap Perolehan Kadar Senyawa Fenolat Total Dan Daya Antioksidan Dari Daun Dewa (Gynura pseudochina(L.) DC) Krisyanella Krisyanella; Andriani Amran; Harrizul Rivai
Jurnal Farmasi Higea Vol 4, No 1 (2012)
Publisher : STIFARM Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.063 KB) | DOI: 10.52689/higea.v4i1.61

Abstract

Study about comparative influence of ethanol – water as solvent extraction of phenolic compounds from Dewa leaves (Gynura pseudochina (L) DC ) and it’s antioxidant activity has been done. The solvent ratios tested were 100:0; 80:20; 70:30; 60:40; 50:50. The result showed that the ratio of ethanol – water used as solvent has a significant correlation to an extractable material, phenolic compound and it’s antoxidant activity (P <0.05 ). The best solvent obtained by ethanol – water of 60 : 40, which gave the highest phenolic compound and antioxidant activity.
Perbandingan Kadar Senyawa Fenolat Dan Daya Antioksidan Pada Teh Celup Dengan Teh Kiloan Dari Beberapa Produk Teh Yang Beredar Roslinda Rasyid; Dinul Aufa; Krisyanella Krisyanella
Jurnal Farmasi Higea Vol 3, No 2 (2011)
Publisher : STIFARM Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.485 KB) | DOI: 10.52689/higea.v3i2.52

Abstract

The determination of phenolic compounds and it’s antioxidant activity of a few tea samples of sacked tea and unsacked tea which is found at the market has been done. The Acquisition of phenolic compound content done by using Folin-Ciocalteau method and it’s antioxidant activity measurement using DPPH as an oxidant (free radical), gallat acids used as the comparison. The results showed that four samples tested unsacked tea KA has the strongest antioxidant activity among the other three samples, because It has the lowest IC50 value. The  IC50 value influenced by it’s phenolic concentration. The phenolic content from unsacked tea KA obtained 39.5487 mg/g, while on samples of tea sacked KA, sacked SW, and unsacked 2K respectively:  19.754 mg/g; 33.9279 mg/g ; and 27.9814 mg/g.
Penentuan Pengaruh Jenis Pelarut Pengekstrak Terhadap Perolehan Kadar Senyawa Fenolat Dan Aktifitas Antioksidan Dari Daun Jambu Biji (Psidium guajava L.) Harrizul Rivai; Rizky Yulion Putra; Krisyanella Krisyanella
Jurnal Farmasi Higea Vol 4, No 1 (2012)
Publisher : STIFARM Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.306 KB) | DOI: 10.52689/higea.v4i1.57

Abstract

Influence of type of extracting solvent on determination of phenolic compound level and antioxidant activities of Guava Leaf (Psidium Guajava, L.) have been done. The determination of phenolic compound by using Folin-Ciocalteau method and Antioxidant activities by using DPPH method that Gallic Acid was used as reference and determination of phenolic compound and antioxidant activities by using UV-Visible spectrophotometry. The content of phenolic compound from methanol:water extract; ethanol:water extract; aceton:water extract are 82.2699; 81.4762 and 80.9206 mg/g respectively. The finding showed that methanol: water solvent is the best solvent for Phenolat extract compare with ethanol:water and aceton:water. Antioxidant activities from methanol:water extract: ethanol:water ; aceton:water are IC50 3.694; 8.249 and 4.968 mg/mL respectively. From the data can get conclusion that methanol:water is the best solvent for extracting Guava Leaf (Psidium Guajava, L.).
Isolasi Senyawa Kimia Utama Dari Fraksi Aktif Sitotoksik Spon Laut Petrosia sp (MN05) Dian Handayani; Corry Handayani; Krisyanella Krisyanella
Jurnal Farmasi Higea Vol 4, No 1 (2012)
Publisher : STIFARM Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (251.992 KB) | DOI: 10.52689/higea.v4i1.58

Abstract

Isolation of major compound (CH-05-SP) from cytotoxic fraction of marine sponge Petrosia sp (MN 05) using maceration, fractionation, column chromatography and recrystallization methods has been conducted. The result of physical and chemical analysis showed that the isolated compound was white crystal, melted at 180º-183ºC and including as terpenoid.