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All Journal Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Ilmu Ternak International Journal of Integrated Health Sciences Majalah Kedokteran Bandung Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Jurnal Ilmiah Teknik Elektro Komputer dan Informatika (JITEKI) Althea Medical Journal Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Perikanan dan Kelautan Jurnal Farmasi Klinik Indonesia Global Medical and Health Communication Pharmauho: Jurnal Farmasi, Sains, dan Kesehatan The Indonesian Biomedical Journal Creative Research Journal Majalah Farmasetika Jurnal Kedokteran Hewan Jurnal Agripet
Mas Rizky A.A. Syamsunarno
Fakultas Kedokteran Universitas Padjadjaran
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Journal : Jurnal Agripet

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Perbandingan Viabilitas Oosit Domba Pasca Vitrifikasi dengan Menggunakan Hemistraw dan Cryotop Kikin Winangun; Rini Widyastuti; Mas Rizky Anggun Adipurna Syamsunarno
Jurnal Agripet Vol 17, No 2 (2017): Volume 17, No. 2, Oktober 2017
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v17i2.8131

Abstract

ABSTRAK.Tujuan dari penelitian ini adalah untuk mengkaji efek vitrifikasi dengan menggunakan dua buah system carrier yang berbeda terhadap viabilitas oosit domba yang telah dimaturasi secara in vitro. Oosit dibagi menjadi dua kelompok perlakuan, yaitu (i) divitrifikasi dengan menggunakan hemistraw (ii) divitrifikasi dengan menggunakan cryotop. Viabilitas oosit dievaluasi berdasarkan reekspansi, warna dan homogenitas sitoplasma. Hasil penelitian ini menunjukkan bahwa viabilitas oosit setelah vitrifikasi serupa pada kedua jenis carrier yang digunakan untuk vitrifikasi. Oosit diletakkan dalam larutan equilibrasi yang mengandung konsentrasi permeable krioprotektan setengah dari larutan vitrifikasi. Setelah 15 menit, oosit ditransfer ke dalam media vitrifikasi yang mengandung 17% EG+17% DMSO +0, 65M sukrosa di dalam modified PBS yang disuplementasi dengan 20% fetal bovine serum. Total waktu yang digunakan untuk memaparkan oosit ke dalam larutan vitrifikasi adalah 30 detik. 5-8 oosit dipipet menggunakan kapiler gelas dan diletakkan/loading ke dalam carrier yang digunakan (hemistraw atau cryotop) kemudian langsung di paparkan ke dalam nitrogen cair. Viabilitas oosit dievaluasi berdasarkan reekspansi, warna dan homogenitas sitoplasma. Hasil penelitian ini menunjukkan bahwa viabilitas oosit setelah vitrifikasi serupa pada kedua jenis carrier yang digunakan untuk vitrifikasi(Comparation of sheep oocyte viability after vitrification using hemistraw and cryotop)ABSTRACT.The aim of this study was to examine the effect vitrification using two different carrier system on the matured sheep oocytes viability. Oocytes were devided into two group (i) vitrified using hemistraw (ii) vitrified using cryotop. Oocytes placed into equilibration solution which is containing a half concentration permeable cryoprotectant of vitrification solution. After 15 minute, oocytes were transferred into vitrification solution containing 17% EG+17% DMSO +0, 65M sucrose in modified PBS supplemented with 20% fetal bovine serum. The total exposure time of oocytes to vitrification solution was 30 sec. Oocytes were pipetted into a glass capillary into group 5-8, and loaded into carrier (hemistraw or cryotop) then plugged into liquid nitrogen. After a week cryopreservation, oocytes were warmed and cultured in TCM 199 suplemented with 10% fetal bovine serum at 38.50 C under 5% CO2 for 3h. Oocytes viability was evaluated by re expansion, color and homogeneity of oocyte cytoplasm. Our results indicated that the oocytes viability after vitrification was similar from both of carrier for vitrification
Pengaruh Keberadaan Corpus Luteum terhadap Kualitas dan Tingkat Maturasi Oosit Domba Lokal Umur Pubertas Awal Secara In Vitro Widyastuti, Rini; Syamsunarno, Mas Rizky Anggun Adipurna; Yusuf, Alvin; Ridhlo, Muhammad Rosyid; Prastowo, Sigit
Jurnal Agripet Vol 18, No 2 (2018): Volume 18, No. 2, Oktober 2018
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v18i2.12103

Abstract

ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh keberadaan corpus luteum (CL) pada ovarium domba umur pubertas awal terhadap kualitas oosit hasil koleksi dan tingkat maturasinya secara in vitro (IVM). Sebanyak 279 oosit digunakan, terbagi pada kelompok tanpa CL (CL-) sebanyak 143 dan dengan CL (CL+) sebanyak 136. Oosit dipilih berdasarkan homogenitas sitoplasma dan dikelompokkan sesuai jumlah lapisan sel kumulus yaitu grade 1 (4 lapis), grade 2 (34 lapis) dan grade 3 (02 lapis). Media IVM menggunakan Tissue Culture Media 199 ditambah antibiotik, Follicle Stimulating Hormone, dan 10% Fetal Bovine Serum. Maturasi dilakukan pada inkubator 38,5C, 5% CO2 selama 24 jam. Pasca IVM, tingkat kematangan oosit dievaluasi berdasar kemunculan Polar Body I (PB I). Hasil menunjukkan bahwa keberadaan CL tidak berpengaruh terhadap kualitas oosit yang dikoleksi pada semua grade. Keberadaan CL berpengaruh pada tingkat kematangan oosit pada grade 1 sebesar 48,64% dibandingkan CL- sebesar 47,19% (p0,05). Berdasarkan hasil penelitian disimpulkan bahwa berpengaruh pada tingkat kematangan oosit setelah IVM. Hasil penelitian menggambarkan potensi penggunaan oosit ternak umur pubertas awal untuk digunakan lebih lanjut dalam program produksi embrio secara in vitro.(Effect of the presence of corpus luteum on oocytes quality and in vitro maturation rate of ewes at early puberty)ABSTRACT. This study aims to know effect of presence of corpus luteum (CL) to collected oocytes quality and its maturation rate post in vitro maturation (IVM), on local ewes ovary at early puberty. In total 279 oocytes were collected, 143 without CL (CL-) and 136 with CL present (CL+). Oocytes were selected according to sitoplasma hemogenity, divided into 3 grades according to cumulus cell (CC) layer namely Grade 1, 2 and 3 indicated by 4, 34, and 02 CC layers, respectively. The IVM media was Tissue Culture Media 199 supplemented with antibiotic, Follicel Stimulating Hormone, and 10% Fetal Bovine Serum following culture at 38.5C and 5% CO2. Twenty four hours post IVM, oocytes were evaluated on the presence of Polar Body I. Result showed that oocytes quality were not different among group in all grades. The present of CL gives better maturation rate in grade 1 compared to CL- (48.64 vs 47.19%; p0.05). The present finding show that the presence of CL improves oocytes maturation rate post IVM. Moreover, this study shows the potency of using oocytes from ewes ovary at early puberty for further in vitro embryo production program
Co-Authors Ahmad Kurniawan Aida Riyanti Alia, Fenty Alif Bagus Rakhimullah Alkaustariyah Lubis Alkaustariyah Lubis Alkaustariyah Lubis Alvin Yusuf Anisah Dahlan Arief Boediono Ariefqi, Muhammad Naufal Aziiz Mardanarian Rosdianto Aziiz Mardanarian Rosdianto Deru Indika Deru R Indika Dinar F. Agustin Djunaedi, Diah Dhianawaty Dwi Cipto Budinuryanto Dwi Wahyudha Wira Edhyana Sahiratmadja Erick Khristian Fauziah, Nisa Gita Widya Pradini Grace Angelica Hanna Goenawan Henhen Heryaman Husneni Mukhtar Irma M. Puspitasari Irma Melyani Puspitasari Isa Mahendra Iwan Setiawan Jaqueline Sudiman Jaya, Fidi Bhawana Jeri Nobia Purnama Julia Windi Gunadi Julius Pangayoman Kikin Winangun Kikin Winangun Lubis, Alkaustariyah Madeline Priscilla Maratul Mahdiyyah Masahiko Kurabayasi Mohammad Ghozali Mohammad Ghozali, Mohammad Muhammad Nurhalim Shahib Muhammad Rosyid Ridhlo Neni Anggraeni Nia Kania Norisca A. Putriana Norisca Aliza Putriana Nova Sylviana Ramdan Panigoro Rangga Setiawan Rangga Setiawan Ratu Safitri Ratu Safitri Ridhlo, Muhammad Rosyid Rikho Melga Shalim Rini Widyastuti Ronny Lesmana Roro Wahyudianingsih Rosdianto, Aziiz Mardanarian Sigit Prastowo Sigit PRASTOWO Sony Heru Sumarsono Sony Heru Sumarsono Sony Heru Sumarsono SONY HERU SUMARSONO Sudiman, Jaqueline Sulistiyorini, Ifa Syah Putra, Heru TAKDIR SAILI Tatsuya Iso Tyagita Hartady Tyagita Tyagita Unang Supratman Uni Gamayani Winangun, Kikin Yulin Prisdiany Yuni Susanti Pratiwi Yusuf, Alvin