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All Journal Journal of Biomedicine and Translational Research Mutiara Medika: Jurnal Kedokteran dan Kesehatan Biomedika KOVALEN: Jurnal Riset Kimia Medika Tadulako Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Media Medika Muda Medika Alkhairaat : Jurnal Penelitian Kedokteran dan Kesehatan Healthy Tadulako Journal (Jurnal Kesehatan Tadulako) Jurnal Kedokteran STM (Sains dan Teknologi Medik) Jambura Medical and Health Science Journal Molekul: Jurnal Ilmiah Kimia Biomedika
David Pakaya
Departemen Histologi Fakultas Kedokteran Universitas Tadulako, Palu
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Docking Study of Naphthalene Compounds from Eleutherine Bulbosa as Antidiabetic Agents on Multiple Receptors Anam, Syariful; Yuyun, Yonelian; Yusriadi, Yusriadi; Sultan, Asriana; Pakaya, David; Maulana, Saipul; Zubair, Muhammad Sulaiman
Molekul Vol 20 No 1 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.1.10942

Abstract

ABSTRACT. Diabetes Mellitus is a severe disease to the world health community; it is estimated that 700 million people worldwide will suffer from it. The approach taken to this disease includes improving lifestyle and pharmacological therapy. Recent pharmacological therapeutic approaches include inhibiting the α-glucosidase enzyme, the dipeptidyl peptidase 4 (DPP-4) enzyme, and the sodium-glucose co-transporter-2 (SGLT-2) protein. This research aims to conduct a docking study on three naphthalene compounds from Eleutherine bulbosa against three receptors: α-glucosidase, the DPP-4, and the SGLT-2 protein. The methods used are protein structure preparation, docking protocol validation, preparation of E. bulbosa test ligand structures, and molecule docking for test compounds. Validation was carried out by calculating the Root Mean Square Deviation (RMSD) values using PyMOL software; the results showed that the RMSD value of native ligands was <2Å. Molecular docking of the test compounds was conducted using Autodock Vina 1.2.3 ver 2020. Eleutherinoside A showed the highest binding against the α-glucosidase and DPP-4 protein. Eleuthoside B has the most increased binding to SGLT-2 protein. The research concluded that three naphthalene compounds from E. bulbosa can be used as an antidiabetic agent. Keywords: Antidiabetic; Docking study; Eleutherine bulbosa; Naphthalene; α-glucosidase; DPP-4; SGLT-2
EFEK METFORMIN TERHADAP GAMBARAN HISTOPATOLOGI GINJAL TIKUS MODEL OBESITAS Pakaya, David; Gusti Ngurah Andika Bhaskara Pinatih; Wira Amaz Gahari
Medika Alkhairaat: Jurnal Penelitian Kedokteran dan Kesehatan Vol 7 No 03 (2025): Desember
Publisher : Fakultas Kedokteran Universitas Alkhairaat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31970/ma.v7i03.341

Abstract

ABSTRAK Peradangan pada obesitas dapat menyebabkan peningkatan sitokin pro-inflamasi sehingga memicu hipertrofi kerusakan pada tubulus ginjal. Pemberian metformin pada penderita obesitas dapat menurunkan produksi sitokin pro-inflamasi dan mencegah terjadinya kerusakan ginjal. Penelitian ini bertujuan mengetahui efek metformin terhadap berat badan dan gambaran histopatologi ginjal pada tikus model obesitas. Penelitian eksperimental dengan rancangan posttest only controlled group design dengan pendekatan kualitatif dan kuantitatif, menggunakan tikus putih galur Wistar jantan, usia 10-12 minggu, berat badan (BB) 200-250 gram, berjumlah 15 ekor. Tikus dibagi dalam 3 kelompok perlakuan; K1: kontrol normal; K2: kontrol negatif (model obesitas); K3: model obesitas + terapi metformin 250 mg/kgBB. Tikus dimodifikasi menjadi model obesitas dengan pemberian high fat diet (HFD). Dilakukan pengukuran BB secara berkala. Gambaran histopatologi ginjal didapatkan dari blok parafin dengan pewarnaan Hematoksilin eosin (HE) dan dianalisis secara kualitatif dengan menilai tingkat kerusakan tubulus ginjal dan analisis kuantitatif dilakukan dengan Grapphad Prsima 8.0.0 menggunakan uji nonparametrik Kruskal-Walis. Hasil terapi metformin menurunkan berat badan pada K3 mulai hari ke-42 (p=0,0049). Skor cedera tubulus ginjal lebih rendah dibandingkan K2, namun tidak berbeda secara statistik (p=0,9). Metformin mampu menurunkan berat badan dan mencegah kerusakan tubulus ginjal tikus model obesitas. ABSTRACT Obesity is a chronic disease resulting from the accumulation of excess body fat. Obesity can lead to an increase in pro-inflammatory cytokines that can cause injury of kidney tubule. Giving metformin to obese can reduce the production of pro-inflammatory cytokines and prevent the occurrence of tubule injury. The aim of this study to determine the effect of metformin on body weight and kidney histopathological of obese rat model. This is a experimental research with posttest only controlled group design with qualitative and quantitative approaches. This study used 15 male Wistar strain white rats, aged 10-12 weeks, body weight (BB) 200-250 grams. The rats were divided into 3 groups; K1: normal control; K2: negative control (obesity model); K3: obesity model + metformin therapy 250 mg/kgBB. The rats were modified into models of obesity with high fat diet (HFD)administration. Periodic BB measurements are carried out. The histopathological kidney is obtained from the paraffin bloc and Hematoxylin eosin (HE) staining. The data were analyzed qualitatively by assessing the degree of renal tubules injury and quantitative analysis was carried out by assessing the level of kidney damage using Grapphad Prsima 8.0.0 with nonparametric Kruskal-Wallis test. Metformin therapy decrease weight on K3 starting on day 42, (p=0.0049). The renal tubule injury score was lower than K2, but there was not statistically different (p=0,9). Metformin were able to decrease weight and prevent kidney tubule injury in obese rats model
Co-Authors ADITYA KURNIAWAN Anak Agung Istri Sri Wiadnyani Antari, Arlita Leniseptaria Anwar, Sarifuddin Arlita Leniseptaria Antari Armadhari, Inna Aryoko Widodo Asriana Sultan Christin Rony Nayoan Dewi, Marzela Dion Solli Ruruktipa Eileen Euides Eka Mutiara Herman Fahira, Aliyah Rezky Farid Indra Gunawan Felandina, Yeni Fitriani, Junjun Gabriella Bamba Ratih Lintin Gusti Ngurah Andika Bhaskara Pinatih Haerani harun Ibrahim, Elfiana Imtihanah Amri Indah Saraswati Ira Cinta Lestari Joni Tandi, Joni Ketut Suarayasa Laurents Christovel Iban Demen Maramis, Maynard Andrew Marzela Dewi Merlionarsy Tammuan Mohammad Fiqri Novian Affandy Mohammad Salman Muhammad Nur Ikhsan Liwang Muhammad Sulaiman Zubair Muhammad Yoland Muliadi Niluh Puspita Dewi, Niluh Puspita Qosimah, Dahliatul Rais Trisiyambudi Rina Susilowati Saipul Maulana Sarifuddin Anwar Sarifuddin Anwar Solehah, Puput Octaviani Syariful Anam Tarusu, Fikhta Agnesya Tinta, Iniche Tri Setyawati Wira Amaz Gahari Wiwit Setyowati Yonelian Yuyun Yuli Fitriana Yusriadi Yusriadi Yustina Andwi Ari. Sumiw