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Bioactive Pigments of Monascus purpureus: Identification and Characterization Shaleha, Resha Resmawati; Yuliana, Anna; Rahmiyani, Ira; Pebiansyah, Anisa; Indriyanti, Indriyanti
Journal of Food and Pharmaceutical Sciences Vol 13, No 4 (2025): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.22574

Monascus purpureus is a filamentous fungus that has long been recognized as one of the main producers of natural pigments. These pigments, including yellow (ankaflavin, monascin), orange (rubropunctatin, monascorubrin), and red (rubropunctamine, monascorubramine), are widely used as natural coloring agents. Compared to synthetic dyes, Monascus pigments are considered safer and possess additional biological activities, making them attractive for applications in the food, pharmaceutical, and cosmetic industries. Therefore, accurate identification of the pigments in Monascus purpureus is essential to ensure their safety, functionality, and potential industrial applications. The aim of this research is to determine the results of pigment identification Monascus purpureus using UV-Vis Spectrophotometry, Fourier Transform Infra-Red (FTIR), Thin Layer Chromatography (TLC), Preparative Thin Layer Chromatography (Preparative TLC), and Liquid Chromatography- Mass Spectrometry (LC-MS). The method used is method laboratory experiments. Samples are tested using several instruments. The extraction results show that the % yield value meets the requirements, namely >10%, then the fractionation results show that the water fraction gets more thick extract, the TLC results for the water fraction show a spot height of 4.7 cm with a value of Rf 0.78, the results of Preparative TLC on the water fraction show the presence of a single fluorescent band at wavelengths of 254 nm and 366 nm. The UV-Vis spectrophotometry results were identified at a wavelength of 477 nm with an absorbance of 0.364, then the FTIR results on Preparative TLC scrapings from the water fraction showed the presence of functional groups from the orange pigment structure and the LC-MS results showed that the sample was a compound. Monascorubrin (C23H28O5) with a molecular weight of 384.99 g/mol and compound rubropunctatin (C21H22O5) with a molecular weight of 354.74 g/mol. These results provide information that the sample is an orange pigment from Monascus purpureus with two compounds monascorubrin and rubropunctatin.

Bioactive Pigments of Monascus purpureus: Identification and Characterization Shaleha, Resha Resmawati; Yuliana, Anna; Rahmiyani, Ira; Pebiansyah, Anisa; Indriyanti, Indriyanti
Journal of Food and Pharmaceutical Sciences Vol 13, No 4 (2025): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.22574

Monascus purpureus is a filamentous fungus that has long been recognized as one of the main producers of natural pigments. These pigments, including yellow (ankaflavin, monascin), orange (rubropunctatin, monascorubrin), and red (rubropunctamine, monascorubramine), are widely used as natural coloring agents. Compared to synthetic dyes, Monascus pigments are considered safer and possess additional biological activities, making them attractive for applications in the food, pharmaceutical, and cosmetic industries. Therefore, accurate identification of the pigments in Monascus purpureus is essential to ensure their safety, functionality, and potential industrial applications. The aim of this research is to determine the results of pigment identification Monascus purpureus using UV-Vis Spectrophotometry, Fourier Transform Infra-Red (FTIR), Thin Layer Chromatography (TLC), Preparative Thin Layer Chromatography (Preparative TLC), and Liquid Chromatography- Mass Spectrometry (LC-MS). The method used is method laboratory experiments. Samples are tested using several instruments. The extraction results show that the % yield value meets the requirements, namely >10%, then the fractionation results show that the water fraction gets more thick extract, the TLC results for the water fraction show a spot height of 4.7 cm with a value of Rf 0.78, the results of Preparative TLC on the water fraction show the presence of a single fluorescent band at wavelengths of 254 nm and 366 nm. The UV-Vis spectrophotometry results were identified at a wavelength of 477 nm with an absorbance of 0.364, then the FTIR results on Preparative TLC scrapings from the water fraction showed the presence of functional groups from the orange pigment structure and the LC-MS results showed that the sample was a compound. Monascorubrin (C23H28O5) with a molecular weight of 384.99 g/mol and compound rubropunctatin (C21H22O5) with a molecular weight of 354.74 g/mol. These results provide information that the sample is an orange pigment from Monascus purpureus with two compounds monascorubrin and rubropunctatin.

PENYULUHAN PENANGGULANGAN DAN PENCEGAHAN STUNTING, PHBS, DAN GIZI SEIMBANG PADA ANAK-ANAK Shaleha, Resha Resmawati; Famili, Dinda Nurhayati; Azzahra, Syifa Rizki; Rahima, Nazmi; Agustin, Ana Sopiana; Wildan, Muhamad Maulana
JMM (Jurnal Masyarakat Mandiri) Vol 8, No 5 (2024): Oktober
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v8i5.25675

Abstrak: Stunting adalah hambatan tumbuh pada anak dengan gangguan tumbuh kembang anak yang dikarenakan oleh minimnya asupan nutrisi, infeksi, atau perasaan tidak mencukupi. Tujuan dari pengabdian masyarakat ini yaitu untuk meningkatkan kemampuan dalam hal komunikasi, kepemimpinan, dan manajemen waktu yang diaplikasikan melalui pemberian edukasi kepada masyarakat, khususnya kepada anak-anak yang tinggal di daerah, tentang pentingnya pola makan seimbang dan pola hidup bersih dan sehat dalam mencegah stunting. Penyuluhan ini dilaksanakan untuk mengedukasi masyarakat di Desa Cayur, Kecamatan Cikatomas, mengenai pencegahan dan penanggulangan stunting melalui pola hidup bersih dan sehat (PHBS) serta asupan gizi seimbang. Metode yang digunakan adalah penyuluhan langsung kepada 30 peserta dengan beragam latar belakang pendidikan, di mana materi disampaikan dalam bentuk media cetak (leaflet) dan audio visual. Hasil evaluasi menunjukkan peningkatan pengetahuan peserta dari 65% (pre-test) menjadi 79% (post-test), yang mencerminkan efektivitas metode penyuluhan. Peningkatan kesadaran dan pemahaman mengenai pentingnya gizi seimbang dan PHBS diharapkan dapat mengurangi prevalensi stunting di desa tersebut.Abstract: Stunting is a growth obstacle in children with growth and development disorders caused by minimal nutritional intake, infection, or feelings of inadequacy. The aim of this community service is to improve skills in terms of communication, leadership and time management which are applied through providing education to the community, especially to children living in the area, about the importance of a balanced diet and a clean and healthy lifestyle in preventing stunting. This counseling was carried out to educate the community in Cayur Village, Cikatomas District, about preventing and overcoming stunting through a clean and healthy lifestyle (PHBS) and balanced nutritional intake. The method used was direct counseling to 30 participants with various educational backgrounds, where the material was delivered in the form of print media (leaflets) and audio-visual. The evaluation results showed an increase in participants' knowledge from 65% (pre-test) to 79% (post-test), which reflects the effectiveness of the counseling method. Increasing awareness and understanding of the importance of balanced nutrition and PHBS is expected to reduce the prevalence of stunting in the village.

PENYULUHAN PENCEGAHAN DBD DI PC PERSISTRI KOTA TASIKMALAYA Zain, Dichy Nuryadin; Pebiansyah, Anisa; Yuliana, Anna; Amin, Saeful; Rahmiyani, Ira; Alifiar, Ilham; Hidayat, Taufik; Shaleha, Resha Resmawati
JMM (Jurnal Masyarakat Mandiri) Vol 8, No 3 (2024): Juni
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v8i3.23025

Abstrak: Demam Berdarah Dengue (DBD) merupakan salah satu masalah kesehatan di Indonesia yang disebabkan oleh virus dengue dan ditandai dengan tingginya jumlah nyamuk Aedes aegypti, sehingga mempengaruhi lingkungan dan kemampuan masyarakat dalam mengendalikan penularan DBD di suatu wilayah. Tujuan penyuluhan ini adalah peningkatan pengetahuan dan kesadaran masyarakat dalam mencegah penularan DBD. Proses penyuluhan mencakup persiapan, instruksi edukasi DBD, pengenalan aplikasi android RESIX, pemasangan paket tempat sampah, penyerahan alat kebersihan, diskusi dan evaluasi kegiatan melalui kuisioner pretest dan postest yang terdiri dari lima soal pada 110 ibu-ibu di PC Persistri Kota Tasikmalaya. Hasil evaluasi menunjukkan pengetahuan tentang DBD meningkat secara signifikan, dengan peningkatan skor sebesar 24.43%.Abstract: Blood dengue fever is a health problem in Indonesia that is part of a type of infectious disease caused by the dengue virus and is transmitted through aegypti mosquito bite characterized by high heat.Environmental factors as well as the role of society in the efforts to prevent the occurrence of DBD disease are closely related to the occurrence of DBD in a region.The purpose of this dissemination is to increase public knowledge and awareness in preventing the transmission of DBD.Preparation methods include preparation, DBD education, Introduction to Android Resix, Installation of garbage packages, submission of hygiene equipment as well as discussion and evaluation of activities through a pretest and postest query of up to 5 questions on 110 persistry PC mothers.The evaluation results showed a significant increase in knowledge of DBD with an increase in score by 24.43%.

EDUKASI STUNTING DENGAN PANDUAN MAKANAN GIZI SEIMBANG MENGGUNAKAN BUKU DIGITAL DAN AUDIO VISUAL DI LKSA AMANAH KOTA TASIKMALAYA Rahmiyani, Ira; Shaleha, Resha Resmawati; Hati, Revita Permata; Tisnawati, Eva; Harningsih, Rini; Assidik, Rizki Abdurochman
JMM (Jurnal Masyarakat Mandiri) Vol 8, No 2 (2024): April
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v8i2.22164

Abstrak: Stunting merupakan kondisi gagal tumbuh kembang dan masalah terkait gizi yang disebabkan oleh asupan gizi yang kurang. Penderita stunting memiliki postur tubuh lebih pendek dari standar usianya, mudah sakit, serta memiliki produktivitas yang rendah pada saat dewasa. Penerapan pencegahan stunting pada anak-anak merupakan hal penting yang harus terus digerakkan sebagai upaya penurunan nilai prevalensi stunting. Dengan demikian, diharapkan angka kejadian stunting di Indonesia dapat ditekan. Kegiatan pengabdian masyarakat ini bertujuan untuk memberikan edukasi terkait stunting dan upaya pencegahannya melalui pola makan gizi seimbang menggunakan buku digital dan audio visual. Kegiatan pengabdian ini mampu meningkatkan kesadaran masyarakat tentang pentingnya mencegah stunting dengan pemenuhan gizi seimbang dan dengan edukasi melalui buku digital serta audio visual akan mudah dipahami oleh masyarakat. Kegiatan pengabdian kepada masyarakat ini dilakukan di LKSA Amanah Tasikmalaya dengan dihadiri jumlah responden 63 orang. Media yang digunakan berupa buku digital dan audio visual terkait stunting serta gizi seimbang, dan juga pemberian kuesioner dengan jumlah pertanyaan 10 soal. Hasil penilaian menggunakan kuesioner dengan metode pre-post test menunjukan adanya peningkatan dari 68,37% menjadi 88,90%. Sehingga terdapat peningkatkan pengetahuan responden terhadap penegtahuan stunting dan pola pemilihan makanan kandungan gizi seimbang.Abstract: Stunting is a condition of failure to grow and develop and problems related to nutrition caused by insufficient nutritional intake. Stunting sufferers have a body posture that is shorter than standard for their age, get sick easily, and have low productivity as adults. Implementing stunting prevention in children is an important thing that must continue to be carried out as an effort to reduce the prevalence of stunting. In this way, it is hoped that the number of stunting incidents in Indonesia can be reduced. This community service activity aims to provide education regarding stunting and efforts to prevent it through a balanced nutritional diet using digital books and audio visuals. This service activity is able to increase public awareness about the importance of preventing stunting by providing balanced nutrition and education through digital books and audio visuals that will be easily understood by the public. This community service activity was carried out at LKSA Amanah Tasikmalaya, attended by 63 respondents. The media used were digital books and audio visuals related to stunting and balanced nutrition, as well as providing a questionnaire with a total of 10 questions. The results of the assessment using a questionnaire with the pre-post test method showed an increase from 68.37% to 88.90%. So there is an increase in respondents' knowledge regarding stunting awareness and food selection patterns with balanced nutritional content.

PENYULUHAN KESEHATAN TENTANG PENYIMPANAN DAN PEMBUANGAN OBAT KEDALUWARSA Shaleha, Resha Resmawati; Zaniera, Zaniera; Aliifah, Maitsa Wafaa; Dwiputri, Nadya; Dewi, Riska Nurmala; Tisnawati, Eva; Marliyani, Mila; Rachma, Diva
JMM (Jurnal Masyarakat Mandiri) Vol 9, No 3 (2025): Juni
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v9i3.31210

Abstrak: Kesehatan masyarakat sangat dipengaruhi oleh perilaku individu dalam mengelola obat di rumah untuk keperluan swamedikasi. Penyimpanan dan pembuangan obat kedaluwarsa yang tidak memenuhi standar menimbulkan resiko serius, seperti penyalahgunaan, keracunan dan dampak negatif terhadap lingkungan yang diakibatkan oleh limbah farmasi. Tujuan kegiatan ini yaitu menambah pemahaman masyarakat berupa penyimpanan dan pembuangan obat kedaluwarsa yang benar. Kegiatan pengabdian masyarakat ini menggunakan metode berupa penyuluhan kesehatan dari tim mahasiswa dengan masyarakat kelurahan Setiaratu yang berjumlah 65 orang peserta 65 orang. Kegiatan ini dilakukan di Kelurahan Setiaratu, Kecamatan Cibeureum, Kota Tasikmalaya. Evaluasi yang dilakukan pada kegiatan ini dilihat dari meningkatnya skor hasil post-test dibandingkan dengan pre-testyang terdiri dari 10 pertanyaan mencakup 5 soal dengan pilihan jawaban ganda dan 5 soal lainnya berupa pilihan jawaban “Ya” dan “Tidak”. Hasil penyuluhan menunjukkan bahwa sebelum kegiatan, sebagian besar peserta dengan persentase 96,92% memiliki skor rendah (3–4) pada pre-test, dan hanya 3,08% yang mencapai skor sedang (6–8). Setelah penyuluhan, seluruh peserta memperoleh peningkatan pengetahuan dengan persentase 100% pada saat post-test. Hasil uji Paired T-Test juga memperlihatkan kenaikan skor rata-rata dari 4.03 menjadi 10.00, menunjukkan peningkatan pengetahuan yang signifikan. Secara keseluruhan, penyuluhan tentang penyimpanan dan pembuangan obat kedaluwarsa berhasil meningkatkan pemahaman masyarakat secara positif.Abstract: Public health is greatly influenced by individual behavior in managing drugs at home for self-medication purposes. Storage and disposal of expired drugs that do not meet standards pose serious risks, such as misuse, poisoning and negative impacts on the environment caused by pharmaceutical waste. This activity aims to increase public knowledge about the correct way to store and dispose of expired drugs. This community service activity uses a method in the form of health counseling from a student team with 65 participants. This activity was carried out in Setiaratu Sub-district, Cibeureum District, Tasikmalaya City. The evaluation carried out in this activity was seen from the increase in the post-test score compared to the pre-testwhich consisted of 10 questions including 5 questions with multiple choice answers and 5 other questions in the form of "Yes" and "No" answer choices. The results of the counseling showed that before the activity, most participants with a percentage of 96.92% had a low score (3-4) on the pre-test, and only 3.08% achieved a medium score (6-8). After counseling, all participants obtained an increase in knowledge with a percentage of 100% at the post-test. Paired T-Test also showed an increase in the mean score from 4.03 to 10.00, indicating a significant increase in knowledge. Overall, the counseling on the storage and disposal of expired medicines succeeded in positively improving the community's understanding.

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