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IDENTIFICATION OF HUMAN HAIR USING DIRECT PCR TARGETING THE CYTOCHROME B GENE Tri Widodo, Wimbuh; Masjkur, Indah Nuraini; Huda, Qurrota A’yunil; Yudianto, Ahmad; Kristianto, Sonny; Putri, Rury Erina
Meditory : The Journal of Medical Laboratory Vol 12, No 2 (2024): Meditory, Volume 12 No. 2 Tahun 2024
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v12i2.3784

Background: Hair is one of the human parts that is easily scattered and easy to obtain for testing. Direct PCR which is PCR without going through the DNA extraction process has been widely used in various fields. The cytochrome b gene is one of the genes located in mitochondrial DNA. The gene is unique in that it has a small variation within one species organism but has a large variation between species. Aims: c. Methods: Hair was heated at 600C for 10 minutes then the sample was used for the PCR template. PCR was performed using the cytochrome b gene. The results: The analysis showed that human hair could still be detected on day 30 using direct PCR using the cytochrome b gene. Conclusions: This result will simplify and save time in analysis in various fields related to hair shafts.

Pendampingan dan Pelatihan Pemanfatan Sampah Popok Bayi di Desa Karangtalun Kecamatan Kalidawir, Tulungagung Widodo, Wimbuh Tri; Kristianto, Sonny; Putri, Rury Eryna; Aprilia, Chatrina; Rahmadani, Anindita Mutiara; Martha, Rahma Diyan; Danar, Danar; Kumalasari, Nurul Chamidah
Jurnal Pengabdian Masyarakat (ABDIRA) Vol 5, No 3 (2025): Abdira, Juli
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/abdira.v5i3.914

Environmental issues caused by baby diaper waste have become a major concern in Karangtalun Village, Tulungagung, due to the low public awareness of its impact. This community service aimed to enhance residents’ knowledge and skills in processing diaper waste into useful planting media. The methods applied included education, training, and demonstrations. Residents were educated on the environmental impact of waste and trained to produce planting media from used baby diapers using the kokedama technique. The results showed improved public understanding of waste management and growing interest in utilizing waste as a business opportunity. This activity also had a positive impact on environmental cleanliness and local economic potential. These findings demonstrate that community-based waste management initiatives are effective in improving environmental quality and community welfare.

Penguatan Kualitas Kesehatan Masyarakat dan Perekonomian di Desa Wates Tulungagung Berbasis Kearifan Lokal Berupa Jamu Brotowali Martha, Rahma Diyan; Danar, Danar; Putri, Amalia Eka; Setyaningrum, Dyah; Nurin, Wachidatun; Fadila, Natasya Nurul; Widodo, Wimbuh Tri
Jurnal Kreativitas Pengabdian Kepada Masyarakat (PKM) Vol 7, No 11 (2024): Volume 7 No 11 (2024)
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkpm.v7i11.17122

ABSTRAK Kegiatan ini dilakukan di Desa Wates, Kecamatan Sumbergempol, Kabupaten Tulungagung, untuk menangani minimnya pemanfaatan tanaman herbal, khususnya brotowali, yang memiliki potensi besar sebagai obat tradisional. Tujuan kegiatan ini adalah untuk mengevaluasi dampak program pemberdayaan masyarakat terkait pemanfaatan tanaman herbal ini. Metode penelitian meliputi penyuluhan, pelatihan, dan pendampingan dalam pembuatan jamu, serta penanaman tanaman herbal di pekarangan rumah. Hasil penelitian menunjukkan peningkatan signifikan dalam pengetahuan dan minat masyarakat tentang tanaman herbal dan pembuatan jamu. Sebelum program, 40% responden memiliki tanaman herbal di pekarangan rumah, sedangkan setelah program, 100% responden melaporkan adanya tanaman herbal di pekarangan mereka. Keinginan untuk menanam tanaman herbal juga meningkat dari 80% menjadi 100%. Kesimpulan dari penelitian ini adalah bahwa program pemberdayaan masyarakat berhasil meningkatkan pengetahuan, keterampilan, dan motivasi masyarakat dalam memanfaatkan tanaman herbal. Kata Kunci: Pemberdayaan Masyarakat, Tanaman Herbal, Brotowali, Pembuatan Jamu ABSTRACT This activity was carried out in Wates Village, Sumbergempol District, Tulungagung Regency, to address the minimal use of herbal plants, especially brotowali, which has great potential as a traditional medicine. The aim of this activity is to evaluate the impact of community empowerment programs related to the use of this herbal plant. Research methods include counseling, training and assistance in making herbal medicine, as well as planting herbal plants in the home yard. The research results show a significant increase in people's knowledge and interest about herbal plants and making herbal medicine. Before the program, 40% of respondents had herbal plants in their yard, while after the program, 100% of respondents reported having herbal plants in their yard. The desire to plant herbal plants also increased from 80% to 100%. The conclusion of this research is that the community empowerment program has succeeded in increasing people's knowledge, skills and motivation in using herbal plants. Keywords: Community Empowerment, Herbal Plants, Brotowali, Making Herbal Medicine.

Detection of Escherichia Coli Using PCR Analysis Without DNA Extraction Widodo, Wimbuh Tri; Huda, Choirul
Folia Medica Indonesiana Vol. 57, No. 2
Publisher : Folia Medica Indonesiana

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This study aimed to detect Escherichia coli directly without DNA extraction. The nucleus membrane and cell membranes of the Escherichia coli are composed of a phospholipid bilayer, damaged if heated at 950C. Pre-denaturation and denaturation of PCR were carried out at 950C. The two stages are thought to break down the Escherichia coli cells, so that the DNA that comes out of the cells can directly become a template in the PCR analysis. In this study, PCR analysis was carried out using Escherichia coli culture, Escherichia coli bacteria culture incubated at 950C, and Escherichia coli bacteria cultures incubated at 650C + on ice as templates. The results showed that PCR analysis using Escherichia coli culture directly and Escherichia coli culture incubated at 650C + on ice as templates produced very thin DNA bands with a size of 580 bp. while PCR analysis using Escherichia coli bacteria culture incubated at 950C as a template produced thick DNA bands with a size of 580 bp. This study's results are very useful for saving time and costs in the detection of Escherichia coli bacteria. The sample to be tested does not need DNA isolation as usual, but only needs to be incubated at 950C for 10 minutes.

Identification of Human DNA in Mixture of Human and Chicken Blood Using PCR with Specific Primer of Cytochrome B Gene Widodo, Wimbuh Tri; Yudianto, Ahmad; Wahyuningsih, Astuti
Folia Medica Indonesiana Vol. 54, No. 3
Publisher : Folia Medica Indonesiana

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This study aimed to identify human DNA from mixing human and chicken blood samples by utilizing Polymerase Chain Reaction (PCR) and cytochrome b gene primer. The cytochrome b gene is a gene located in mitochondrial DNA and has high variation of sequence relation between one species and another. PCR analysis was performed using human cytochrome b gene primer in variation of DNA templates (0 ng, 0.01 ng, 0.1 ng, 1 ng, 10 ng and 100 ng), human blood percentages (100%, 50%, 40 %, 25%, 10%, 5%, 1%, 0%) and sample age before analysis (0 day, 3 days, 7 days, 10 days, and 15 days). The minimum DNA template obtained in this study was 0.01 ng and minimum percentage of human blood in the mixture was 1%. Blood spots on cloth isolated on days 0, 3, 7, 10 and 15 could still be analyzed and the resulting of DNA band (157 bp) had the same intensity/thickness. From the results of this study, it can be concluded that human blood in the mixture of human and chicken blood can be identified using PCR with specific primers of cytochrome b gene. PCR using specific primer of cytochrome b gene may help forensic practitioners to identify human sample in mixed biological samples.

Petroleum Degradation by Bacteria Explored from Logending Mangrove Sediments Apriliani, Herlina; Pramono, Hendro; Oedjijono, Oedjijono; Widodo, Wimbuh Tri; Kristianto, Sonny; Putri, Rury Eryna; Unsunnidhal, Lalu
Biota Vol 12 No 1 (2026): Jurnal Biota 2026
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v12i1.29245

Oil spills resulting from shipping activities, tanker-based oil transportation, and fuel oil usage can cause coastal pollution, particularly in sensitive ecosystems such as mangroves. More than 90% of petroleum consists of hydrocarbons with complex carbon chain structures, making them difficult to decompose. Biological remediation using microorganisms offers a promising alternative for pollution mitigation, as microbes can degrade petroleum components and oxidizing hydrocarbons. This study aimed to evaluate the petroleum-degrading ability of selected bacterial isolates obtained from mangrove sediments at Logending Beach. The research employed experimental and survey methods. The primary parameter measured was Total Petroleum Hydrocarbons (TPH), while supporting parameters included pH and bacterial population density. The study consisted of several stages, including bacterial isolation, screening, and evaluation of the petroleum degradation capacity of selected isolates. The results identified two potential bacterial isolates capable of degrading crude oil. Isolate LG62 exhibited a degradation efficiency of 71.40%, while isolate LG105 showed a degradation efficiency of 57.10%. Petroleum concentrations of 2% (v/v) and 5% (v/v) were degraded more effectively than higher concentrations. Overall, the two bacterial isolates (LG62 and LG105) from Logending mangrove sediments demonstrated significant potential as bioremediation agents for petroleum hydrocarbon contamination.

Etnofarmakologi Etnis Madura: Kajian Obat Herbal Tradisional untuk Pengobatan Gejala Tuberkulosis Kristianto, Sonny; Putri, Rury Eryna; Widodo, Wimbuh Tri
Metamorfosa: Journal of Biological Sciences Vol. 12 No. 1 (2025)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2025.v12.i01.p4

Tuberkulosis (TBC) merupakan salah satu penyakit menular yang disebabkan oleh infeksi bakteri Mycobacterium tuberculosis (MTB) dan menyebar melalui udara. Selain relatif mahal dan akses terbatas, fenomena resistensi TBC terhadap obat menjadi hal umum di negara berkembang termasuk Indonesia. Di sisi lain, obat herbal tradisional Indonesia yang dikenal sebagai jamu telah digunakan sejak lama untuk mengobati gejala penyakit TBC. Suku Madura merupakan salah satu suku di Indonesia yang masih menggunakan tanaman herbal sebagai jamu tradisional. Penelitian ini mengeksplor jenis tanaman obat yang digunakan oleh etnis Madura untuk meramu jamu dan menduga kandungan senyawa aktif dalam jamu yang terlibat dalam pengobatan TBC. Pendekatan deskriptif-kualitatif berupa wawancara mendalam pada masyarakat lokal dilakukan untuk menemukan potensi obat dari etnis Madura serta prediksi aktivitas senyawa aktif untuk penanganan gejala penyakit kronis populer berupa TBC. Hasil penelitian menunjukkan bahwa cara pemanfaatan tanaman obat sebagai jamu tradisional oleh etnis Madura masih mempertahankan budaya warisan leluhur dengan menggunakan peralatan tradisional yang terbuat dari tanah. Selain itu, sebagian besar tanaman obat yang dimanfaatkan oleh etnis Madura tergolong dalam famili Zingiberaceae yang memiliki beragam senyawa aktif dengan aktivitas antioksidan, antimikrob, antiinflamasi bahkan antitusif yang baik untuk mengurangi gejala TBC berupa demam dan batuk. Temuan etnofarmakologi ini menjembatani pengetahuan herbal lokal etnis Madura dengan kajian ilmiah modern, serta menunjukkan potensi jamu tradisional sebagai sumber kandidat terapi pendukung untuk penanganan gejala Tuberkulosis (TBC). Kata kunci: antimikroba, etnis Madura, jamu, senyawa aktif, TBC

Etnofarmakologi Etnis Madura: Kajian Obat Herbal Tradisional untuk Pengobatan Gejala Tuberkulosis Kristianto, Sonny; Putri, Rury Eryna; Widodo, Wimbuh Tri
Metamorfosa: Journal of Biological Sciences Vol. 12 No. 1 (2025)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2025.v12.i01.p4

Tuberkulosis (TBC) merupakan salah satu penyakit menular yang disebabkan oleh infeksi bakteri Mycobacterium tuberculosis (MTB) dan menyebar melalui udara. Selain relatif mahal dan akses terbatas, fenomena resistensi TBC terhadap obat menjadi hal umum di negara berkembang termasuk Indonesia. Di sisi lain, obat herbal tradisional Indonesia yang dikenal sebagai jamu telah digunakan sejak lama untuk mengobati gejala penyakit TBC. Suku Madura merupakan salah satu suku di Indonesia yang masih menggunakan tanaman herbal sebagai jamu tradisional. Penelitian ini mengeksplor jenis tanaman obat yang digunakan oleh etnis Madura untuk meramu jamu dan menduga kandungan senyawa aktif dalam jamu yang terlibat dalam pengobatan TBC. Pendekatan deskriptif-kualitatif berupa wawancara mendalam pada masyarakat lokal dilakukan untuk menemukan potensi obat dari etnis Madura serta prediksi aktivitas senyawa aktif untuk penanganan gejala penyakit kronis populer berupa TBC. Hasil penelitian menunjukkan bahwa cara pemanfaatan tanaman obat sebagai jamu tradisional oleh etnis Madura masih mempertahankan budaya warisan leluhur dengan menggunakan peralatan tradisional yang terbuat dari tanah. Selain itu, sebagian besar tanaman obat yang dimanfaatkan oleh etnis Madura tergolong dalam famili Zingiberaceae yang memiliki beragam senyawa aktif dengan aktivitas antioksidan, antimikrob, antiinflamasi bahkan antitusif yang baik untuk mengurangi gejala TBC berupa demam dan batuk. Temuan etnofarmakologi ini menjembatani pengetahuan herbal lokal etnis Madura dengan kajian ilmiah modern, serta menunjukkan potensi jamu tradisional sebagai sumber kandidat terapi pendukung untuk penanganan gejala Tuberkulosis (TBC). Kata kunci: antimikroba, etnis Madura, jamu, senyawa aktif, TBC

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