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All Journal Jurnal Teknosains JURNAL PERBENIHAN TANAMAN HUTAN Jurnal Ternak Tropika Jurnal Biologi Tropis Diklabio: Jurnal Pendidikan dan Pembelajaran Biologi Jurnal Peternakan (Jurnal of Animal Science) ARSHI Veterinary Letters Vitek : Bidang Kedokteran Hewan Journal of Applied Veterinary Science and Technology Jurnal Biosense Jurnal Karya Abdi Masyarakat Jurnal Ilmiah Ilmu-Ilmu Peternakan Jurnal Pengabdian Tri Bhakti
Damhuri, Dedi
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UJI KUALITATIF KANDUNGAN KARBOHIDRAT DAN PROTEIN PADA BAHAN PANGAN HEWANI DAN NABATI MENGGUNAKAN METODE BIOKIMIA SEDERHANA Bima Reksa Kumala; Dedi Damhuri
JURNAL BIOSENSE Vol 9 No 3 (2026): Edisi Juli 2026
Publisher : Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i3.8186

Abstract

Carbohydrates and proteins are essential macronutrients that play important roles in body metabolism and human nutritional needs. This study aimed to identify the carbohydrate and protein contents in various animal- and plant-based food materials using simple biochemical methods, namely Lugol and Biuret tests. The study employed a qualitative descriptive method with samples consisting of tofu, tempeh, milk, banana, catfish, beef, chicken meat, egg, avocado, and peanuts. Sample preparation was carried out by homogenizing the food materials and adding distilled water to obtain filtrates for testing. The Lugol test was performed to detect starch content based on the formation of a blue-black color, while the Biuret test was used to detect proteins through the formation of a purple Cu–peptide complex. The results showed that banana gave a positive result in the Lugol test indicated by a dark coloration, suggesting the presence of starch, whereas most other samples showed negative results. In the Biuret test, tofu, tempeh, milk, catfish, beef, chicken meat, egg, and peanuts showed positive results indicated by a purple color change, confirming the presence of protein. This study demonstrates that Lugol and Biuret tests can be used as simple, rapid, and effective methods for the preliminary identification of carbohydrate and protein contents in food materials.
REAKSI PERUBAHAN STRUKTUR PIGMEN ANTOSIANIN DAN KURKUMIN PADA KONDISI ASAM DAN BASA Dedi Damhuri; Damar Alhadi Maulana
JURNAL BIOSENSE Vol 9 No 3 (2026): Edisi Juli 2026
Publisher : Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i3.8354

Abstract

Pemanfaatan indikator alami dari bahan tumbuhan menjadi alternatif yang lebih aman, murah, dan ramah lingkungan dibandingkan indikator sintetis dalam pengujian asam dan basa. Penelitian ini bertujuan untuk mengeksplorasi potensi serta efektivitas ekstrak bahan alam, yaitu buah naga (Hylocereus costaricensis), kunyit (Curcuma longa), dan kol ungu (Brassica oleracea L) sebagai indikator alami asam-basa. Ketiga bahan alam tersebut secara berurutan mengeksploitasi sensitivitas pigmen betasianin, kurkumin, dan antosianin terhadap fluktuasi derajat keasaman (pH) lingkungan uji. Metode penelitian dilakukan secara eksperimental melalui ekstraksi bahan alam dan pengujian perubahan warna visual pada tiga sampel representatif, yaitu cuka dapur, minuman karbonat (Sprite), dan deterjen. Hasil penelitian menunjukkan bahwa larutan cuka dapur secara konsisten memberikan respon spektrum warna asam yang valid dan sesuai teori pada seluruh indikator. Namun, pengujian pada sampel Sprite dan deterjen menunjukkan adanya anomali atau pergeseran visual dari trayek warna ideal akibat pengaruh interferensi matriks zat aditif, efek pengenceran, variasi konsentrasi ekstrak, serta tingkat kekuatan disosiasi asam-basa sampel uji. Meskipun perubahan warna akhir tidak selalu bersifat linear layaknya indikator sintetis akibat batasan limitasi deteksi senyawa fungsional, ketiga ekstrak bahan alam ini terbukti mengalami transformasi struktural (protonasi dan deprotonasi) yang aktif. Dengan demikian, dapat disimpulkan bahwa ekstrak buah naga, kunyit, dan kol ungu memiliki potensi besar sebagai instrumen alternatif indikator asam-basa alami yang aplikatif untuk pembelajaran kimia dasar. Abstract This study aims to explore the potential and effectiveness of natural extracts, namely dragon fruit (Hylocereus costaricensis), turmeric (Curcuma longa), and red cabbage (Brassica oleracea L), as natural acid-base indicators. These three natural resources sequentially exploit the sensitivity of betacyanin, curcumin, and anthocyanin pigments toward fluctuations in environmental acidity (pH). The research was conducted experimentally through maceration extraction of the natural materials followed by visual color change testing on three representative samples: household vinegar, a carbonated beverage (Sprite), and detergent. The results demonstrated that the vinegar solution consistently produced a valid and theoretically compliant acidic color spectrum response across all indicators. However, the testing on Sprite and detergent samples exhibited anomalies or visual shifts from the ideal color charts, which were attributed to the interference of additive matrices, dilution effects, variations in extract concentration, and the dissociation strength of the tested samples. Although the final color transitions were not always linear compared to synthetic indicators due to the detection limitations of the functional compounds, all three natural extracts were proven to undergo active structural transformations (protonation and deprotonation). Consequently, it can be concluded that dragon fruit, turmeric, and red cabbage extracts possess substantial potential as applicable alternative instruments for natural acid-base indicators in basic chemistry education.
UJI AKTIVITAS ANTIBAKTERI EKSTRAK KULIT NANAS TERHADAP Escherichia coli MULTIRESISTEN DARI PETERNAKAN KAMBING PEMATANG GAJAH JAMBI DEDI DAMHURI
Jurnal Peternakan (Jurnal of Animal Science) Vol 10, No 2 (2026): JURNAL PETERNAKAN (JURNAL OF ANIMAL SCIENCE)
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jas.v10i2.24156

Abstract

Escherichia coli merupakan bakteri Gram-negatif yang secara alami hidup sebagai mikroflora normal pada saluran pencernaan hewan dan manusia, namun beberapa strain patogen dapat menyebabkan penyakit enterik, septikemia, serta infeksi sistemik. Meningkatnya kejadian resistensi antibiotik pada bakteri patogen di lingkungan peternakan mendorong pengembangan alternatif antibakteri yang berasal dari bahan alami. Kulit nanas (Ananas comosus) diketahui mengandung berbagai senyawa bioaktif seperti flavonoid, tanin, polifenol, saponin, serta enzim proteolitik bromelain yang berpotensi memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengevaluasi aktivitas antibakteri ekstrak kulit nanas terhadap bakteri Escherichia coli multiresisten yang diisolasi dari peternakan kambing di Desa Pematang Gajah, Jambi. Penelitian dilakukan menggunakan metode eksperimental laboratorium dengan teknik difusi cakram pada media Mueller Hinton Agar. Ekstrak kulit nanas diperoleh melalui metode maserasi menggunakan pelarut etanol dan diuji pada konsentrasi 15%, 30%, 45%, 60%, 75%, dan 90%. Gentamicin digunakan sebagai kontrol positif dan pelarut steril sebagai kontrol negatif. Hasil penelitian menunjukkan bahwa ekstrak kulit nanas mampu menghambat pertumbuhan Escherichia coli pada seluruh konsentrasi yang diuji. Diameter zona hambat meningkat seiring dengan peningkatan konsentrasi ekstrak hingga mencapai aktivitas optimum pada konsentrasi 75% dengan diameter zona hambat sebesar 17,82 ± 0,67 mm, sedangkan konsentrasi 15% menunjukkan aktivitas terendah dengan diameter zona hambat sebesar 6,12 ± 0,41 mm. Konsentrasi 90% menunjukkan aktivitas antibakteri yang lebih tinggi dibandingkan konsentrasi 60%, namun masih lebih rendah dibandingkan konsentrasi 75%. Hasil penelitian menunjukkan bahwa ekstrak kulit nanas memiliki potensi sebagai sumber antibakteri alami terhadap bakteri Escherichia coli multiresisten.
Computational screening of boswellic acid for its antibacterial activity against acne-causing bacteria via molecular docking Dedi Damhuri; Ietje Wientarsih; Rini Madyastuti Purwono; Agustin Indrawati; Asri Rizky; Jessica Anggun Safitri; Ahmad Syarifuddin
Jurnal Teknosains Vol 15, No 1 (2025): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/teknosains.106278

Abstract

Acne vulgaris (AV) is a common inflammatory-skin disorder associated with bacterial infections, particularly Cutibacterium acnes, Staphylococcus aureus, and Staphylococcus epidermidis. The rising resistance to conventional antibiotics has prompted the exploration of natural compounds such as boswellic acid, which is known for its antibacterial potential. This study aimed to evaluate the antibacterial activity of boswellic acid using an in-silico approach through molecular docking against several essential bacterial target proteins implicated in acne pathogenesis. The boswellic acid ligand was obtained from the PubChem database, while the three-dimensional structures of the target proteins were retrieved from the RCSB Protein Data Bank. Blind docking was performed using AutoDock Tools version 1.5.7 and AutoDock Vina, followed by interaction analysis using Discovery Studio Visualizer and Visual Molecular Dynamics (VMD). Nine bacterial proteins involved in vital cellular processes such as metabolism, protein synthesis, biofilm formation, and DNA replication were selected, including transcriptional regulator TcaR, penicillin-binding proteins (PBPs), tyrosyl-tRNA synthetase (TyrRS), 3-ketoacyl-ACP synthase III (KAS III), CRISPR-associated protein, DNA gyrase, transcriptional regulator MarR, methylmalonyl-CoA epimerase, and accumulation-associated protein (Aap). The docking results demonstrated that all target proteins exhibited negative binding energy values (< 0), indicating thermodynamically stable and spontaneous interactions. Among these, TcaR displayed the highest binding affinity with a binding energy of −10.2 kcal/mol and formed nine conventional hydrogen bonds, reflecting a particular and stable interaction. Key interacting residues included Gln: B61, HisA:42, AsnA:20, AsnB:17, and Arg1:110. In contrast, the Aap protein formed only one covalent bond, indicating the weakest interaction. These findings suggest that boswellic acid effectively inhibits key bacterial proteins, particularly those involved in transcriptional regulation and biofilm development. Therefore, boswellic acid holds significant potential as a safe and effective topical antibacterial agent for further growth in biomedical engineering-based formulations.
IDENTIFIKASI JENIS MANGROVE DI KAWASAN PANGKAL BABU, DESA TUNGKAL 1, KECAMATAN TUNGKAL ILIR, TANJUNG JABUNG BARAT JAMBI riris roiska; Dedi Damhuri; Putinur
JURNAL BIOSENSE Vol 9 No 1 (2026): Edisi Januari 2026
Publisher : Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i1.6819

Abstract

Mangroves are coastal forest ecosystems commonly distributed in tropical and subtropical regions and play a vital role in maintaining coastal environmental stability. The mangrove ecosystem in Pangkal Babu, Tungkal 1 Village, Tungkal Ilir District, West Tanjung Jabung Regency, Jambi, functions as an ecological buffer for the coastal zone; however, scientific information on its species composition and diversity remains limited. This study aimed to identify and document the mangrove species present in the area. Field surveys and direct observations were conducted to characterize mangrove vegetation by recording and photographing each species and examining diagnostic features, including root structure, stem morphology, leaf shape, and fruit characteristics. The results showed that the Pangkal Babu mangrove ecosystem is composed of several true mangrove species, each exhibiting distinctive morphological traits: Acanthus ilicifolius with thick spiny leaves; Nypa fruticans with large pinnate leaves and clustered fruits; Sonneratia alba with erect pneumatophores and subglobose fruits; Avicennia alba with salt-excreting leaves and pencil-shaped pneumatophores; Bruguiera cylindrica with knee roots and cylindrical propagules; Rhizophora mucronata with mucronate leaves and large stilt roots; Rhizophora apiculata with black-spotted leaves and shorter propagules; and Xylocarpus granatum with compound leaves and large spherical fruits resembling cannonballs. These findings provide essential baseline data for future conservation efforts, habitat monitoring, and sustainable coastal management initiatives in Tungkal Ilir and the surrounding regions.
PENGARUH KONSENTRASI BOSWELLIC ACID TERHADAP MUTU FISIK SEDIAAN NANO GEL BERBASIS KARBOPOL 940 Dedi Damhuri; Sri Novianti; Fatati; Jul Andayani
JURNAL BIOSENSE Vol 9 No 2 (2026): Edisi April 2026
Publisher : Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i2.7338

Abstract

Nanotechnology represents an innovative strategy to enhance the penetration and efficacy of active compounds in topical formulations. This study aimed to formulate and evaluate the physicochemical properties of Boswellic Acid (BA) nano-gel with varying concentrations of the active ingredient using a Carbopol 940-based matrix. The research methodology commenced with particle size reduction of BA through an ultrasonication technique for 150 minutes, followed by its integration into a gel matrix containing propylene glycol and glycerin as humectants. The pharmaceutical quality evaluation included organoleptic, homogeneity, pH, viscosity, spreadability, and drying time tests. The results demonstrated that all formulations (NgBA-F1, NgBA-F2, and NgBA-F3) exhibited excellent homogeneity with a constant pH value of 5, ensuring compatibility with skin physiology. The viscosity of all formulas reached 3000 cPa, meeting the ideal standards, although the concentration of the active ingredient significantly influenced the color intensity of the preparation. However, mechanical testing revealed a spreadability of 3 cm, which did not meet the ideal criteria of 5–7 cm due to high viscosity. Furthermore, the addition of BA extended the drying time to a range of 26.27–28.17 seconds compared to the gel base (15.65 seconds). This study concludes that the Boswellic Acid nano-gel formulation met the physical quality standards for pH, viscosity, and homogeneity, but requires further optimization of the base components to improve spreadability characteristics and drying time kinetics.
Antibiotic Resistance of Escherichia coli and Staphylococcus aureus Bacteria in Goats in Pematang Gajah, Jambi Dedi Damhuri; Yatno Yatno; Asri Rizky; Jessica Anggun Safitri; Ahmad Syarifuddin
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11678

Abstract

Limited knowledge among smallholder goat farmers regarding appropriate antibiotic use may contribute to the emergence of antimicrobial resistance in livestock-associated bacteria. This study aimed to identify bacterial isolates and evaluate antibiotic resistance patterns of bacteria obtained from fecal and nasal mucosal swabs of goats in Pematang Gajah. Samples were collected from nine goats with three repeated examinations. Bacterial identification was performed using Gram staining followed by standard biochemical tests. The results showed that 8 of 9 isolates (≈90%) from fecal swabs exhibited biochemical characteristics consistent with Escherichia coli, including Indole (+), MR (+), VP (−), Citrate (−), TSIA A/A with gas production, positive motility, and metallic green sheen on EMB agar. A total of 7 of 9 isolates (≈76%) from nasal mucosal swabs demonstrated typical characteristics of Staphylococcus aureus, namely Gram-positive cocci, catalase-positive, coagulase-positive, mannitol fermentation positive, and β-hemolysis. Antibiotic susceptibility testing conducted on one isolate of E. coli and one isolate of S. aureus with three replications revealed the highest inhibition zones in E. coli against enrofloxacin (23.33 ± 0.58 mm) and gentamicin (21.33 ± 0.58 mm), categorized as sensitive, while high resistance was observed against tylosin (8.33 ± 0.58 mm) and penicillin–streptomycin (9.33 ± 0.58 mm). Staphylococcus aureus isolates showed high sensitivity to enrofloxacin (24.33 ± 0.58 mm), tylosin and gentamicin (22.33 ± 0.58 mm), but resistance to penicillin–streptomycin and ampicillin. These findings indicate variability in antimicrobial susceptibility among goat-associated bacteria and highlight the importance of susceptibility-based antibiotic selection to reduce antimicrobial resistance risk.
Diagnosis and treatment of deep pyoderma in a Maine Coon cat Dedi Damhuri; Sarwo Edy Wibowo; Ahmad Syarifuddin; Jessica Anggun Safitri; Ani Insulistyowati; Amelia Tri Ningsih
ARSHI Veterinary Letters Vol. 8 No. 4 (2024): ARSHI Veterinary Letters - November 2024
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.8.4.77-78

Abstract

Pyoderma is a condition caused by a bacterial infection that affects the skin and has the potential to cause systemic infection. A Maine Coon cat was reported to have an open wound on its tail that was wet and oozing fluid, with the cat frequently biting and scratching the affected area. Based on the anamnesis, physical examination, and clinical symptoms, the Maine Coon cat was diagnosed with deep pyoderma. The treatment administered included antibiotics, vitamins, anti-inflammatory drugs, and antifungals. The cat underwent therapy for 30 days, showing signs of healing as the wound began to dry, although it had not yet fully closed. Supportive therapy with vitamins was continued to promote significant recovery. It was concluded that the treatment of deep pyoderma in the Maine Coon cat resulted in wound drying by day 30.
Co-Authors Agustin Indrawati Ahmad Syarifuddin Ahmad Syarifuddin ahmad syarifuddin Amelia Tri Ningsih Amelia Triningsih Triningsih Ani Insulistyowati Asri Rizky Awaludin, Aan Bayu Rosadi Bima Reksa Kumala Damar Alhadi Maulana Eka Sri Wahyuni, Eka Fahmida Faozan Indresputra Fatati Febrina Rolin Ietje Wientarsih Irdam Idrus Jessica Anggun Safitri Jul Andayani Jumiarni, Dewi Madyastuti, Rini Nugraheni, Yudhi Ratna Nurhayani Pudji Rahayu Puti Puspa Seruni Putinur Ratna Sholatia Harahap riris roiska Sarwo Edy Wibowo Shaddiq Rosa, Teuku Sri Novianti Sri Rahayu Sri Wigati Suradi Suradi Vega Decline Yanita Mutiaraning Viastika Yatno Yatno Yatno, Yatno Yuniar Yuniar