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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Indonesian Journal of Agricultural Science Jurnal AgroBiogen AGRIVITA, Journal of Agricultural Science Jurnal Biologi Tropis Jurnal Pertanian Agros Jurnal Agrotek Tropika Journal of Tropical Crop Science Jurnal Bioindustri BIOVALENTIA: Biological Research Journal Journal of Comprehensive Science Agrotech Journal Journal of Bioindustry
Awang Maharijaya
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (IPB University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Energy Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Medicine & Pharmacology

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Journal : Journal of Tropical Crop Science

 1 
In Vitro Polyploidy Induction of Patchouli (Pogostemon Cablin Benth.) by Colchicine Afifah, Ulil Azmi Nurlaili; Wiendi, Ni Made Armini; Maharijaya, Awang
Journal of Tropical Crop Science Vol 7 No 01 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (515.055 KB) | DOI: 10.29244/jtcs.7.01.37-44

Abstract

Patchouli (Pogostemon cablin Benth.; 2n = 32) is an aromatic herbaceous plant commonly cultivated for use in the the fragrance industry. As patchouli is propagated by cuttings polyploidization induction by colchicine treatment was conducted to obtain a new genotype with high patchouli alcohol content. This research aimed to increase patchouli genetic diversity with colchicine treatment by the formation of a polyploid plant. Axillary buds from single node cutting of aseptic plantlets were used as the explants. The experiment was arranged using a factorial completely randomized design with two factors, namely the concentration of colchicine and the immersion duration. Genetic diversity of patchouli was successfully enhanced by adjusting the colchicine concentration and immersion treatment. lethal concentration (LC) of 50% in patchouli was 0.132% and the LC 50% for soaking time was 60.16 hours. The number of chloroplasts, stomatal length and chromosome number increased with increasing ploidy, whereas stomatal density and the number of trichomes decreased. The chromosome number of 16 patchouli mutants in generation 4 was still unstable, and a chimera was found with mixoploidy between 20-80. A hierarchical dendogram clustered 16 patchouli mutants into four different groups.
Histological Analysis of Somatic Embryogenesis from Immature Zygotic Embryo of Wild Banana Musa acuminata ssp. malaccensis Handayani, Tri; Martanti, Diyah; Prawestri, Apriliana Dyah; Tihurua, Eka Fatmawati; Maharijaya, Awang; Wahyu, Yudiwanti; Sobir; Witjaksono
Journal of Tropical Crop Science Vol. 11 No. 03 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.03.307-319

Abstract

Somatic embryogenesis, a crucial plant regeneration method, has become indispensable for crop improvement, particularly for species reliant on somatic cell manipulation techniques. Optimization of this process necessitates an understanding of the developmental stages involved. This study investigates the histological aspects of somatic embryogenesis in Musa acuminata ssp. malaccensis derived from immature zygotic embryos. Through detailed histological analysis, we aimed to elucidate the morphological changes and cellular organization occurring during the various stages of somatic embryogenesis, from induction, culture proliferation, and somatic embryo development to plantlet conversion. The initial stages of embryogenesis, characterized by nodules, were primarily composed of meristematic cells with high cell division activity. These cells contained tetrad-like structures that could develop into distinct two- and four-celled proembryoids or proembryogenic aggregates. Our histo-anatomical analysis revealed that embryogenic cultures proliferated through multiple pathways simultaneously: somatic embryo budding, proembryo formation, and pro-embryonic mass formation from both internal and peripheral cells. At the stage of somatic embryo development, embryos with a well-defined protoderm layer, containing cells with prominent nuclei and dense cytoplasm, potentially regenerate into plantlets. Furthermore, histological examination revealed the presence of procambium within mature somatic embryos, which subsequently developed into the vascular system of the complete plantlet
An Efficient Somatic Embryogenesis and Plant Regeneration from Immature Embryo of Wild Banana Musa acuminata ssp. malaccensis Handayani, Tri; Martanti, Diyah; Prawestri, Apriliana Dyah; Maharijaya, Awang; Wahyu, Yudiwanti; Sobir; Witjaksono
Journal of Tropical Crop Science Vol. 12 No. 01 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.01.172-184

Abstract

Wild banana Musa acuminata ssp. malaccensis, an ancestor of cultivated bananas, possesses valuable genetic diversity, including resistance genes to fusarium wilt, and demonstrates high environmental adaptability. These traits are important for pre-breeding programs, whether by conventional breeding, which is a lengthy process, or by taking advantage of somatic cell manipulation techniques such as somatic hybridization, which requires an efficient plant regeneration system like somatic embryogenesis. We have established an efficient and comprehensive protocol for somatic embryogenesis of this wild Musa using immature zygotic embryo explants covering culture induction and proliferation, somatic embryo development, and subsequent plant conversion. Embryogenic culture was induced on a complex-modified MS medium supplemented with 1 mg.L-1 2,4-D auxin or 5 mg.L-1 picloram. The embryogenic cultures proliferated in the form of granular or nodular structures, which was best obtained by reducing the picloram concentration to 1 mg.L-1 and combining it with the same concentration of 2,4-D at a half-strength macro-nutrient of basal medium. Embryo development from embryogenic cultures and regeneration into shoots. Proembryos as granular structures spontaneously matured into early-stage somatic embryos upon withdrawal of the strong auxin inducer. Increasing the sucrose and gelling agent concentrations in the growth medium improved somatic embryo formation from embryogenic cultures. The frequency of shoot formation from developed somatic embryos was increased by incorporating 0.5 mg.L-1 BA and 0.5 mg.L-1 GA3 into the regeneration medium.
Co-Authors ,, Rosminah Abdul Hakim Ady Daryanto Afaf, Sausanil Agus Purwito Ahmad Fauzi Ridwan Alveno, Vitho Amalia Nurul Huda Amarilis, Shandra Amrullah, Rizki Abi Anas Dinurrohman Susila Arum Sholikhah Azhari, Andi Bagas Akmala Putra Bambang Sapta Purwoko Bonjok Istiaji Danu Kuncoro Dhiya Nabilla Ardhani Diny Dinarti Diyah Martanti, Diyah Dwinita Wikan Utami Eka Fatmawati Tihurua Endang Gunawan Eny Widajati Erna Siaga, Erna Erna Sinaga Erviana Eka Pratiwi Fazat Fairuzia Hariyadi Harti, Heri Hidayat, dan Purnama Ika Roostika Inayah Yasmin Kamila Iswari Saraswati Dewi Iswari Saraswati Dewi Ita Aprilia J. K. J. Laisina Juharni Kalsum Yulifar, Andi Sri Ummi Ketty Suketi Kristianto Nugroho Kunio Yamada Kusmana Kusuma Darma Kusuma Darma Lina Herlina Lina Herlina M A Chozin Marlin Marlin Masaki Ochiai Megayani Sri Rahayu Muhamad Syukur Muhammad Mahmud Muhammad Ridha Alfarabi Istiqlal Neng Neni Neni, Neng Ni Made Armini Wiendi Ni Made Armini Wiendi, Ni Made Niken Kendarini Nina Agusti Widaningsih Nursalma, Linda Prawestri, Apriliana Dyah Prima Muklisa PURNAMA HIDAYAT Purwoko, Bambang Sapta Purwoko Qadir, Abdul Rahmawati, Rika Sri Ramdhani, Cahyati Reflinur Reflinur Reflinur Reflinur Rerenstradika T. Terryana Ria Rif’atunidaudina Riti, Estriana Ritonga , Arya Widura Ritonga, Arya W Ritonga, Arya Widura Rizki Abi Amrullah Romdoni, Arif Rosminah, Rosminah Salma, Linda Nur Sani, Mutiyara Sari Mahyendra Sayekti, Tri Wahono Dyah Ayu Siti Yuliani, Titiek Sobir Sobir Sobir Sobir Sobir Sobir Sobir Sobir SRI HENDRASTUTI HIDAYAT Suhartanto , M. Rahmad Sukma, Dewi Sulassih, . Suryo Wiyono Suwarto Suwarto Syarifah Iis Aisyah Tamrin Khamidi Tamrin Khamidi Tengku Laila Kamaliah Tri Handayani Ulil Azmi Nurlaili Afifah Usep Jenudin Willy Bayuardi Suwarno Witjaksono Yudiwanti Wahyu E. Kusumo Yuliani, Titiek