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All Journal ACTA VETERINARIA INDONESIANA QARDHUL HASAN: MEDIA PENGABDIAN KEPADA MASYARAKAT Jurnal Peternakan Nusantara Seminar Nasional Lahan Suboptimal JURNAL KAJIAN VETERINER Jurnal Nukleus Peternakan Karimah Tauhid Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Abdullah Baharun
Universitas Djuanda
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary Other

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THE POTENCY OF BUNIDA F1 AND THEIR CROSSBREED (BUNIDA F2) AS SUPERIOR LOCAL CHICKEN Rahmi, Annisa; Baharun, Abdullah; Adinda Putri, Tivanka
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.8978

Abstract

High market demands for animal protein have encouraged the optimization of local chicken genetic resources. This research aims to determine the potency of BUNIDA F1 chickens and their crossed-breed (BUNIDA F2). Five BUNIDA F1 hens at 12 months of age are inseminated with Bresse’s semen until they get 50 eggs. The eggs are incubated at 37-38°C and 60-70% humidity. The egg’s fertility and embryo devel-opment were observed on the 4th, 7th, and 18th days of incubation with the candling method. The parameters observed were egg weight, egg fertility, hatchability, and hatching weight. The results show BUNIDA F1 potencies are 54.12 ± 2.70 g egg weight, 82.59 ± 5.11% fertility rate, 71.14 ± 10.67% for hatchability, and hatching weight of BUNIDA F2 is 37.86 ± 1.97 g. It can be concluded that BUNIDA F1 and BUNIDA F2 have the potency to be developed into superior local chickens.
IMPROVING SEMEN QUALITY OF PERANAKAN ONGOLE CATTLE WITH MARIGOLD®-BASED ANTIOXIDANT IN TRIS-EGG YOLK DILUENT Hasan Anwar Hanafi; Abdullah Baharun; Tulus Maulana; Annisa Rahmi; Muhammad Gunawan; Athhar Manabi Diansyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aimed to determine the optimal concentration of Marigold® antioxidant suplemented in a Tris-egg yolk (TEY) extender to maintain the quality of liquid semen from Peranakan Ongole (PO) bulls. Semen samples were extended in TEY without antioxidant (P0, control) or supplemented with Marigold® extract at concentrations of 0.002% (P1), 0.004% (P2), and 0.006% (P3). Semen quality was evaluated based on macroscopic characteristics (volume, colour, pH, and consistency) and microscopic parameters (mass motility, progressive motility, viability, plasma membrane integrity (PMI), and morphological abnormalities). Fresh semen exhibited an average volume of 5.25±0.8 mL, creamy colour, pH 6.8±0.13, mass motility (++), abnormalities 4.1±0.8%, viability 86.12±6.2%, PMI 77.21±5.3%, and progressive motility 85.44±2.41%. Supplementation with Marigold® antioxidant significantly influenced microscopic parameters during storage at 4 °C. Sperm abnormalities increased with storage duration, particularly in P3 on day 2. Treatment with 0.004% Marigold® (P2) effectively maintained motility and PMI up to day 6, while 0.006% (P3) preserved viability and PMI up to day 10. The addition of Marigold® extract to the TEY extender improved the preservation of PO bull semen quality during 10 days of storage at 4 °C. A concentration of 0.004% was optimal for maintaining sperm quality in the short term (up to day 6), whereas 0.006% provided stronger protective effects during prolonged storage (up to day 10).
IMPROVING SEMEN QUALITY OF PERANAKAN ONGOLE CATTLE WITH MARIGOLD®-BASED ANTIOXIDANT IN TRIS-EGG YOLK DILUENT Hasan Anwar Hanafi; Abdullah Baharun; Tulus Maulana; Annisa Rahmi; Muhammad Gunawan; Athhar Manabi Diansyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aimed to determine the optimal concentration of Marigold® antioxidant suplemented in a Tris-egg yolk (TEY) extender to maintain the quality of liquid semen from Peranakan Ongole (PO) bulls. Semen samples were extended in TEY without antioxidant (P0, control) or supplemented with Marigold® extract at concentrations of 0.002% (P1), 0.004% (P2), and 0.006% (P3). Semen quality was evaluated based on macroscopic characteristics (volume, colour, pH, and consistency) and microscopic parameters (mass motility, progressive motility, viability, plasma membrane integrity (PMI), and morphological abnormalities). Fresh semen exhibited an average volume of 5.25±0.8 mL, creamy colour, pH 6.8±0.13, mass motility (++), abnormalities 4.1±0.8%, viability 86.12±6.2%, PMI 77.21±5.3%, and progressive motility 85.44±2.41%. Supplementation with Marigold® antioxidant significantly influenced microscopic parameters during storage at 4 °C. Sperm abnormalities increased with storage duration, particularly in P3 on day 2. Treatment with 0.004% Marigold® (P2) effectively maintained motility and PMI up to day 6, while 0.006% (P3) preserved viability and PMI up to day 10. The addition of Marigold® extract to the TEY extender improved the preservation of PO bull semen quality during 10 days of storage at 4 °C. A concentration of 0.004% was optimal for maintaining sperm quality in the short term (up to day 6), whereas 0.006% provided stronger protective effects during prolonged storage (up to day 10).
Co-Authors Adinda Putri, Tivanka Adipratama, Firza Adistya, Firly Agung Puji Haryanto Agus Setyono Ahmad Faqih Akim, Restu Andri Brawijaya Anggraeni Angraeni, Sinta Annisa Rahmi Annisa Rahmi Annisa Rahmi Annisa Rahmi, Annisa Arasya, Adiba Kanza Athhar Manabi Diansyah Azizah Asma Hamidah BA, Firdaus Boy Surya Adhitya Dede Ilpan Kosasih Dede Kardaya Deden Sudrajat Dewi Wahyuni E. M. Kaiin Feilza Putri, Okta Furqan Herdes, Ali Gagarin, Muhamad Yuri Gumelar, Restu Hasan Anwar Hanafi Ichan Pamungkas Ichsan Pamungkas Ikhsan Qodri Pramartaa Jatmiko Jatmiko Jatmiko Kurniasih, Desta Dwi L. L Belli, Henderiana L. L Belli, Henderiana Lesmana, Dudi Lisna Apriliani Rizkiah Lubis, Wahyuni M. Hasan M. Winugroho Malik, Burhanudin Maman, Asep Masitoh Mohammad Winugroho, Mohammad Muhammad Gunawan Mulyana Mulyana, Dani Naufal, Ahmad Muzaky Ni Wayan Kurniani Karja Nurosidin, Haikal Parida Permadani Pertiwi, Pirda Permadani Pertiwi, Pirda Parida Pirda Parida Permadani Pertiwi Pujiharyanto, Agung R. Handarini R. Iis Arifiantini Raden Mohamad Herdian Bhakti Ristika Handarini Rizal, M. Saman Rohmadi, Agung S. Said Sajidah, Raihanah Seta Astagina Sudrajat, Mashudy Suryani, Danu Syahruddin Said T. Maulana Thomas Mata Hine Tulus Maulana Vetnizah Juniantito Widya Pintaka Bayu Putra Yudi Wahyudin Zahri MF Zandos, F