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Antibiotics Resistance Level of Vibrio spp. Isolated From Northern Bali Area Widya Purnama Dewi; Pande Gde Sasmita Julyantoro; I Wayan Darya Kartika
Advances in Tropical Biodiversity and Environmental Sciences Vol 4 No 2 (2020): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (980.021 KB) | DOI: 10.24843/ATBES.2020.v04.i02.p01

Abstract

One conventional method that usually done when the organism infected by pathogenic bacteria is using antibiotics, either with single or combination usage. However, the misuse of antibiotics dosages leads to resistance development of pathogenic bacteria. This study aims to determine the antibiotic resistance level of Vibrio spp. which was isolated from the waters of North Bali and to investigate the difference of resistance level between Vibrio spp. isolated from the cultivation area and outside the cultivation area. This research was conducted at the Microbiology Laboratory of BKIPM Denpasar and the Laboratory of Fisheries at the Faculty of Marine Science and Fisheries, Udayana University from November 2019 to February 2020. Antibiotic tests were carried out in vitro using 8 types of antibiotics namely tetracycline, oxytetracycline, enrofloxacin, ciprofloxacin, amoxycillin, doxycycline, ampicillin, and erythromycin with different concentrations of 0.5, 1, 2, 4, 8, 16, 32, 64, 64, 128, 256 and 512 ppm. and this test was carried out using a microplate reader to obtain absorbance values before and after incubation to determine the level of resistance of isolated Vibrios. The research showed that overall minimum inhibitory concentration (MIC) of Vibrio was below 100 ppm while the Vibrio spp. isolated from cultivation area have higher resistance level compared to outside cultivation area.
Protection of Thiophenone in Catfish (Clarias sp.) Larvae When Challenged with Aeromonas hydrophila Ana Indriyanti; Pande Gde Sasmita Julyantoro; Ni Putu Putri Wijayanti
Advances in Tropical Biodiversity and Environmental Sciences Vol 5 No 1 (2021): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (645.97 KB) | DOI: 10.24843/ATBES.2021.v05.i01.p04

Abstract

This research aimed to determine the ability of quorum sensing inhibitor compound thiophenone to protect catfish (Clarias sp.) larvae when challenged with the pathogenic Aeromonas hydrophila. This research was conducted at Fisheries Laboratory, Faculty of Marine Science and Fisheries, Udayana University, from December 2019 to January 2020. The experiment was setting up with four treatments and three replications for each treatment. 10 larvae of catfish were maintained in aquarium 15 cm × 15 cm × 20 cm filled with 1 liter of freshwater and used aeration for oxygen supply. The treatments were treatment A (control), treatment B (addition of A. hydrophila 106 cfu/ml), treatment C (addition of thiophenone 10 µM), and treatment D (addition of A. hydrophila 106 cfu/ml and thiophenone 10 µM). The results showed that the addition of thiophenone 10 µM increased the survival of catfish larvae up to 73% when challenged with A. hydrophila. That was significantly difference (P<0,05) compare to treatment B with survival rate of 43% within 3 days of culture period. Although it was not statistically different (P>0,05), the highest absolute weight and length were found in treatment A of 0,47 g and 0,50 cm, respectively, while the lowest absolute weight and length were found in treatment B about 0,23 g and 0,17 cm, respectively. The water quality such as temperature, pH and DO were still within the range that supported the growth and survival of catfish larvae during this study.
Quality of Chaetoceros calcitrans Cultured with Different Concentrations of Potassium Nitrate (KNO3) Alfarisi Prafanda; Pande Gde Sasmita Julyantoro; Ni Putu Putri Wijayanti
Advances in Tropical Biodiversity and Environmental Sciences Vol 4 No 1 (2020): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (593.037 KB) | DOI: 10.24843/ATBES.2020.v04.i01.p02

Abstract

Addition of different fertilizer concentrations into cultivation media affects the cell density of microalgae. Potassium nitrate (KNO3), is one of the components in Guillard fertilizer composition commonly used for microalgae culture. This study aims to determine the quality of Chaetoceros calcitrans when cultured with different concentration of KNO3. This research was conducted from November 2019 to January 2020 at Balai Perikanan Budidaya Air Payau (BPBAP) Situbondo and Biosain Laboratory, Jember State Politeknik, East Java. This study consists of four treatments and three replicates. The object in this study was C. calcitrans culture with initial density 105 cells/ml. The main parameters observed were cell density, biomass, protein and amino acids contents and medium parameters such as temperature, pH, DO, salinity, nitrate and phosphate. There were four concentrations of KNO3 used in this study, namely control (75 g/l), treatment Group A (100 g/l), treatment B (125 g/l) and treatment C (150 g/l). The results showed that the cells density of C. calcitrans at control, treatment Group A, B and C were 110.4; 105.2; 108.3; and 100.8 (×104 cells/ml), respectively. This study indicated that different concentration of KNO3 affect the starting point of stationary phase, but One Way ANOVA test showed that those treatments had no significant effect (P?0,05) on the growth rate and dry biomass of C. calcitrans. Finally, we found that the protein content in addition of 75, 100, 125 and 150 g/l KNO3 were 9.748; 8.802; 6.812; and 3.776%, respectively.
Keanekaragaman dan komposisi jenis plankton pada kolam bioflok ikan nila (Oreochromis niloticus) Ni Kadek Vindi Wedhawati; Pande Gde Sasmita Julyantoro; Dewa Ayu Angga Pebriani
Bumi Lestari Journal of Environment Vol 22 No 2 (2022)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/blje.2022.v22.i02.p02

Abstract

Aquaculture is an activity to cultivate aquatic biota in controlled media. One of the cultivated fish resources is tilapia (Oreochromis niloticus). Tilapia production continues to be increasing to meet increasing of consumer demand. The emergence of the problem of organic matter levels in the water could be harmful to both of fish and the environment. One of the solution to overcome this problem is by application of biofloc technology. The purpose of this study was to determine the diversity and composition of plankton species in tilapia biofloc ponds in Tiga Village, Susut District, Bangli Regency. This research was conducted in March-May 2021 in Tilapia Biofloc Pond. The research used a simple random sampling method. Sampling was carried out once in three biofloc ponds where each pond had 5 points. Data analysis is using the Microsoft Excel program. The data is present in the tables and charts consisting of the abundance, diversity, composition, dominance, and uniformity index of plankton. Based on the research results, it is known that there are 12 genus of phytoplankton and 4 genus of zooplankton. Phytoplankton diversity index values ??ranged from 1.31 to 1.65. Zooplankton diversity index values ranged from 0.79 to 1.08. The composition of phytoplankton species came from the genus Scenedesmus, Chlorella, Coelastrum, Chroococcus, Gonatozygon, Pediastrum, Nitzschia, Navicula, Synedra, Triceratium, Chaetoceros, and Pesudanabaena. The composition of zooplankton species comes from the genus Brachionus, Euchlanis, Tokophyra, and Vorticella. The water quality measured during the study was a temperature value ranging 25 oC, a pH value ranging from 7,12 – 7,18, a DO value ranging from 6.6 to 6.9 mg/L, an ammonia value ranging from 1.41 to 1.51 mg/L, and a nitrate value ranging from 1.84. -3.69 mg/L. This value is still in the optimal range for plankton life.
Kualitas Air, Kelimpahan Mikroba Dan Laju Pertumbuhan Udang Vannamei (Littopenaeus vannamei) Pada Tahap Pembesaran Menggunakan Sistem RAS dan Konvensional Putu Bagaskara; Pande Gde Sasmita Julyantoro; Alfi Hermawati Waskita Sari
Bumi Lestari Journal of Environment Vol 22 No 2 (2022)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/blje.2022.v22.i02.p03

Abstract

Vannamei shrimp (Littopenaeus vannamei) known as white shrimp is widely cultivated in Indonesia because it has high economic value. Conventional cultivation of vannamei shrimp is often represented by poor sewage treatment, where aquaculture waste is discharged directly causing a decrease in water quality in the environment. This study used 2 different cultivation systems, namely RAS (Recirculating Aquaculture System) and conventional systems (flow-through system). The research located at BPIUUK Karangasem, Bali. Water quality monitoring in each tank was carried out including ammonia, nitrite, TSS (Total Suspended Solid), and the abundance of microbes. Measurement of weight and length starts from the age of 30 days of shrimp with further checks every 7 days. A sampling of water quality, as well as measurements of weight and length, were carried out by random sampling method in each rearing tank. Based on the research data, the quality of aquaculture water using RAS and conventional system were not significantly different. The data on the abundance of microbes obtained in cultivation using RAS and conventional were also not significantly different, where RAS reaching 2.0 x 105 cfu/mL, while in conventional system was obtained 2.1 x 105 cfu/mL of total bacteria count. The growth rate with the average value of ADG (Average Daily Growth) in RAS is 0.88 g slightly higher compared to it is 0.80 g in conventional system. This study concluded that cultivation using RAS and conventional by flow-through system were not significantly different in terms of water quality, microbial abundance, and shrimp growth rate.
Kualitas Air, Pertumbuhan dan Kelulushidupan Benih Abalone (Haliotis squamata) dengan Aplikasi RAS di BPIUUK Karangasem Bali Arief Prayoga Akbar; Pande Gde Sasmita Julyantoro; Dewa Ayu Angga Pebriani
Bumi Lestari Journal of Environment Vol 22 No 2 (2022)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/blje.2022.v22.i02.p01

Abstract

Abalone is one of the sea snails that have high economic value and has advantages because it has a high protein content of 71.99%, a fat content of 3.24%, and can be used as medicine. The high demand for abalone causes the increase of wild capture and lead to scarcity of abalone in the waters. One way to overcome this problem is by cultivate the abalone. Abalone cultivation can be done with a monoculture system. One of the technologies applied is by using a recirculation aquaculture system (RAS), which is reprocessing the water used so that it can be reused for cultivation activities. The purpose of this study was to analyze the parameters of water quality, growth length, weight and survival of abalone (Haliotis squamata) seeds using the RAS system. The research was conducted in June – September 2020 at the Shrimp and Shellfish Parent Center, Karangasem, Bali. The research was performed by purposive sampling method. The water quality parameters data such as nitrite, ammonia, TSS pH, temperature, DO, and salinity were taken. The results of the measurement of water quality are nitrite 0 – 0.338 mg/L, ammonia 0 – 0.09 mg/L, TSS 0 – 15 mg/L, pH 7 – 8, temperature 26 – 28 oC, DO 4.3 – 7.07 mg/L, and salinity 31 – 38 ppt. Those values are still in the optimal range for abalone culture. The growth in length and absolute weight of abalone seeds during the study were 25.17 mm/ind. and 9.15 g/ind., respectively, and the survival value was 99.5% during the 100-day culture period.
Penghambatan Faktor Virulensi Vibrio parahaemolyticus Menggunakan Isolat Bakteri dari Saluran Pencernaan Ikan Kerapu Siti Nurlatifah; Ida Bagus Gede Darmayasa; Pande Gde Sasmita Julyantoro; Putu Eka Sudaryatma
Acta VETERINARIA Indonesiana Vol. 10 No. 3 (2022): November 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.3.228-238

Abstract

Ikan kerapu adalah komoditas perikanan yang bernilai ekonomis tinggi pada pasar domestik dan internasional. Akan tetapi, dalam budidaya ikan kerapu masih sering ditemukan kasus penyakit vibrosis yang berdampak terhadap kerugian ekonomi akibat kematian yang ditimbulkan. Penyakit ini disebabkan salah satunya oleh bakteri Vibrio parahaemolyticus yang faktor virulensinya diregulasi oleh mekanisme kuorum sensing (QS). Pencegahan penyakit bakteri selama ini sering dilakukan dengan menggunakan antibiotik yang berisiko munculnya bakteri resisten antibiotik. Salah satu upaya untuk menghindari hal tersebut adalah dengan menghambat sistem QS menggunakan bakteri pendegradasi molekul sinyal N-acyl homoserine lactone (AHL). Pada penelitian ini dilakukan isolasi kandidat bakteri pendegradasi AHL pada media menggunakan AHL komersial sebagai sumber N tunggal dan juga melakukan isolasi bakteri V. parahaemolyticus pada media Thiosulfate-Citrate-BileSalts-Sucrose Agar dari saluran pencernaan ikan kerapu. Pengaruh penambahan isolat kandidat pendegradasi AHL terhadap tiga faktor virulensi V. parahaemolyticus yaitu motilitas, produksi kaseinase dan hemolisa diuji dengan mengkultur V. parahaemolyticus dengan dan tanpa penambahan isolat pendegradasi AHL. Hasil penelitian menunjukkan bahwa 2 isolat kandidat pendegradasi AHL yaitu SNA02 dan SNA03 mampu menurunkan ketiga faktor virulensi yang diuji tersebut. Hal ini mengindikasikan bahwa isolat SNA02 dan SNA03 berpotensi sebagai kandidat bakteri untuk mencegah penyakit vibriosis. Hasil ini diharapkan dapat membantu pembudidaya ikan kerapu dalam mengendalikan patogenitas bakteri V. parahaemolyticus.
APLIKASI PENGGUNAAN SISTEM KANTONG PADA BUDIDAYA RUMPUT LAUT SEBAGAI ALTERNATIF MATA PENCAHARIAN MASA PANDEMI DI PERAIRAN PANTAI KUTUH, BADUNG, BALI A.P.W.K. Dewi; I.W. Restu; P.G.S. Julyantoro; G.R.A. Kartika; N.M. Ernawati
Buletin Udayana Mengabdi Vol 20 No 3 (2021): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (419.643 KB) | DOI: 10.24843/BUM.2021.v20.i03.p15

Abstract

Permasalahan yang dihadapi oleh pembudidaya rumput laut di Pantai Pandawa saat ini adalah rumput laut tidak dapat tumbuh dengan baik. Pertumbuhan rumput laut yang tidak baik disebabkan oleh serangan hama. Solusi yang ditawarkan untuk menyelesaikan masalah tersebut adalah dengan menggunakan metode penanaman yang dimodifikasi dengan penggunaan kantong. Penggunaan kantong bertujuan untuk melindungi rumput laut dari serangan hama yang dapat mengganggu pertumbuhan rumput laut. Metode pelaksanaan yang digunakan dalam kegiatan ini adalah dengan memberikan pelatihan dan mengaplikasikan secara langsung penggunaan kantong pada budidaya rumput laut di perairan Pantai Kutuh. Kegiatan ini bekerjasama dengan pihak manajemen Pantai Pandawa yang dalam hal ini merupakan penanggung jawab Kawasan Pariwisata Pantai Pandawa. Masyarakat yang terlibat adalah sebanyak 13 orang. Kesimpulan dari kegiatan pengabdian ini adalah masyarakat menyambut baik kegiatan pengabdian dan antusias dalam mengikuti kegiatan pelatihan. Selain itu, sebanyak 43% peserta setuju untuk meneruskan budidaya rumput laut dengan metode kantong sebagai mata pencaharian.
THE EFFECT OF Bacillus sp. ADDITION ON THE VIRULENCE OF Vibrio parahemolyticus TOWARD AXENIC CULTURE OF Artemia franciscana Ni Luh Komang Ayu Maitri Jayanthi; Pande Gde Sasmita Julyantoro; Dewa Ayu Angga Pebriani
Journal of Fish Health Vol. 2 No. 1 (2022): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.479 KB) | DOI: 10.29303/jfh.v2i1.1389

Abstract

Research on the benefits of Bacillus sp. in aquaculture has been done a lot. Currently, the aquaculture industry, especially shrimp farming, is facing AHPND disease, which is known to be caused by Vibrio parahaemolyticus. This study aims to see the effect of Bacillus sp. against virulence of V. parahaemolyticus in vitro using caseinase production as an indicator and survival of Artemia franciscana cultured axenically when challenged with V. parahaemolyticus. In in vitro tests, cultures of V. parahaemolyticus were grown with and without the addition of Bacillus sp. on Luria Bertani (LB) liquid media, and then the caseinase test was carried out using skim milk (SM) agar as a growth medium. In the challenge test, 10 axenic cultured Artemia sp. were distributed in falcon tubes containing 10 ml of sterile seawater. Challenge test treatments consisted of treatment A as control of Artemia culture without the addition of bacteria, treatment B Artemia culture adding by 106 CFU/ml V. parahaemolyticus, treatment C Artemia culture with the addition of 106 CFU/ml Bacillus sp., and treatment D Artemia culture with the addition of 106 CFU/ml V. parahaemolyticus and 106 CFU/ml Bacillus sp. All treatments were done in triplicates. The results showed that the addition of Bacillus sp. was able to reduce the caseinase production of V. parahemolyticus up to 29% indicated by the decrease of the clearing zone diameter formed on SM agar. Furthermore, the addition of Bacillus sp. in treatment D was able to significantly increasing (P<0.05) of Artemia's survival when challenged with V. parahamolyticus. This indicates that Bacillus sp. has a potential as a probiotic candidate to prevent disease caused by V. parahaemolyticus.
Laju Pertumbuhan dan Kelulushidupan Ikan Lele Dumbo (Clarias gariepinus) dengan Padat Tebar Berbeda dalam Sistem Bioflok Dian Kharisma Ginting; Pande Gde Sasmita Julyantoro; Ni Putu Putri Wijayanti
Current Trends in Aquatic Science Vol 5 No 1 (2022)
Publisher : Program Studi Manajemen Sumberdaya Perairan, Fakultas Kelautan dan Perikanan, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Biofloc technology is used to overcome the increase in organic matter, improve the quality of aquaculture water, and increase production yields. This study aims to determine the growth rate and survival rate of African catfish with different stocking densities in the biofloc system. This study used a completely randomized design (CRD) with 3 treatments and 5 replications. The test animals used African catfish measuring 7-9 cm with a stocking density of A (15 ind/15L), B (20 ind/15L) and C (25 ind/15L). The results showed that the use of a biofloc system in African catfish culture with different stocking densities resulted in no significant difference in growth rate, survival rate (SR) and feed conversion ratio (FCR). The average weight growth value of African catfish in treatment A, B, and C was 14,13 ± 2,26 g, 13,37±0,46 g, and 12,04±0,48 g, respectively. The average length growth in treatments A, B, and C was 6,27±0,54 cm, 6,09±0,31 cm, and 5,69±0,34 cm, respectively. The survival value (SR) of African catfish in treatment A, B, and C was 85,33±5,58%, 83±2,73%, and 82,40±2,19%, respectively. The value of the feed conversion ratio (FCR) in treatment A, B, and C was 0,72±0,08, 0,75±0,06, and 0,79±0,03, respectively. The range of values for the water quality parameters of the culture media is categorized as suitable for the growth of African catfish, resulting in temperature, DO, and pH value of 27,5-30,8°C, 3,9-5,9 mg/L, pH 6,20-6,45, respectively. The content of ammonia is 0,26-0,44 mg/L, nitrite is 0,08-0,18 mg/L and nitrate is 0,18-0,29 mg/L.
Co-Authors A.H.W. Sari A.P.W.K. Dewi A.P.W.K. Dewi Abrar Ravidhia Ade Angelia Atmanegara Sinaga Agus Wahyudi Alfarisi Prafanda Alfi Hermawati Waskita Alfi Hermawati Waskita Sari Alfi Hermawati Waskita Sari Alfi Hermawati Waskitasari Amayliana Ajeng Nastiti Ana Indriyanti Annas Cesar Mayasha Annisa Millenia Putri Annisa Nur Safitri Utomo Arief Prayoga Akbar Aristiani Rusdi Oktaviyanti Artanti Tri Lestari Ayu Putu Wiweka Krisna Dewi Ayu Putu Wiweka Krisna Dewi Bachori Dhian Pratama Bagus Anjasmara Bedjo Slamet Bona Rizky Simanungkalit Budhi Wahyu Nurindra Claudy Margaretha Kandouw D.A.A. Pebriani Dewa Ayu Angga Pebriani Dewa Ayu Angga Pebriani Dewa Ayu Angga Pebriani Dian Kharisma Ginting Doni Samuel Barus Endang Wulandari Endang Wulandari Suryaningtyas Endang Wulandari Suryaningtyas Errina Bening Ambartyasning Fiko Triajiatma Firda Nurdiana G.R.A. Kartika G.R.A. Kartika Galang Alamin Trisnabatin Gde Raka Angga Kartika Grace Adelina Girsang Handayani Sitorus Hasbia Rahmat Hasrat Damai Hulu I Bagus Andreana Surya Nugraha I Gede Yoga Vikannada Giri I Gusti Ayu Agung Purnama Sari I Gusti Ayu Febiana Putri Wardeni I Gusti Ngurah Permana I Kadek Alamsta Suarjuniarta I Kadek Mega Mega Dhyana Putra I Ketut Wija Negara I Ketut Wija Negara I Komang Yopi Trio Santana I Made Angga Wahyu Dinata I Made Budiarsana I Made Hendy Wesha Suryawan I Wayan Arthana I Wayan Kasa I Wayan Restu I.K.W. Negara I.W. Arthana I.W. Restu Ida Ayu Grisandi Dewi Kurnia Ida Bagus Gede Darmayasa Ilham Muttaqin Jefri Boy Pratama Kadek Bayu Candra Gotama Kartika, I Wayan Darya Luh Gede Manik Radzena Martha M Reza Mei Budi Dharmawan Made Ayu Pratiwi Maria Ulfa Maulana Ilham Pratama N.M. Ernawati N.M. Ernawati N.P.P. Wijayanti Ni Desak Putu Ida Suryani Ni Kadek Vindi Wedhawati Ni Luh Komang Ayu Maitri Jayanthi Ni Luh Putu Ayu Puspita Dewi Ni Made Ernawati, Ni Made Ni Made Putri Handayani Ni Putu Emie Noviana Ni Putu Putri Wijayanti Ni Putu Putri Wijayanti Ni Putu Sandhitya Candra Kartina Ni Putu Tika Lestari Ni Putu Widiantari Peter Bossier Putu Bagaskara Putu Dewi Purnama Sari Putu Eka Sudaryatma Putu Eka Sudaryatma Putu Niken Ayu Saraswati Rai Ayu Ratri Chandra Pandunita Rani Ekawaty Ratriana Hartini Saleh S.A. Saraswati Shanti Paramita Jayanti Siti Nurlatifah Sukarman Sukarman Suprabadevi Ayumayasari Saraswati Suprabadevi Ayumayasari Saraswati Syifa Maria DiazGonzales Tom Defoirdt Wahyu Ilvita Vindia Wahyu Nurlita Widiastuti Widiastuti Widya Purnama Dewi Yan Ramona Yova Tresya Galingging Yufinta Cahya Permanti Yuni Debora Prasicilia Sitorus