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All Journal ACTA VETERINARIA INDONESIANA METAMORFOSA Journal of Biological Sciences Journal of Marine and Aquatic Sciences Buletin Udayana Mengabdi Bumi Lestari Current Trends in Aquatic Science Journal of Marine Research and Technology Journal of Fish Health (JIMBE) Manfish: Jurnal Ilmiah Perikanan dan Peternakan Advances in Tropical Biodiversity and Environmental Sciences

Pande Gde Sasmita Julyantoro

Program Studi Manajemen Sumberdaya Perairan, Fakultas Kelautan Dan Perikanan, Universitas Udayana

Author-ID : 969522

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Kualitas Air, Kelimpahan Mikroba Dan Laju Pertumbuhan Udang Vannamei (Littopenaeus vannamei) Pada Tahap Pembesaran Menggunakan Sistem RAS dan Konvensional Putu Bagaskara; Pande Gde Sasmita Julyantoro; Alfi Hermawati Waskita Sari
Bumi Lestari Journal of Environment Vol 22 No 2 (2022)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/blje.2022.v22.i02.p03

Vannamei shrimp (Littopenaeus vannamei) known as white shrimp is widely cultivated in Indonesia because it has high economic value. Conventional cultivation of vannamei shrimp is often represented by poor sewage treatment, where aquaculture waste is discharged directly causing a decrease in water quality in the environment. This study used 2 different cultivation systems, namely RAS (Recirculating Aquaculture System) and conventional systems (flow-through system). The research located at BPIUUK Karangasem, Bali. Water quality monitoring in each tank was carried out including ammonia, nitrite, TSS (Total Suspended Solid), and the abundance of microbes. Measurement of weight and length starts from the age of 30 days of shrimp with further checks every 7 days. A sampling of water quality, as well as measurements of weight and length, were carried out by random sampling method in each rearing tank. Based on the research data, the quality of aquaculture water using RAS and conventional system were not significantly different. The data on the abundance of microbes obtained in cultivation using RAS and conventional were also not significantly different, where RAS reaching 2.0 x 105 cfu/mL, while in conventional system was obtained 2.1 x 105 cfu/mL of total bacteria count. The growth rate with the average value of ADG (Average Daily Growth) in RAS is 0.88 g slightly higher compared to it is 0.80 g in conventional system. This study concluded that cultivation using RAS and conventional by flow-through system were not significantly different in terms of water quality, microbial abundance, and shrimp growth rate.

Kualitas Air, Pertumbuhan dan Kelulushidupan Benih Abalone (Haliotis squamata) dengan Aplikasi RAS di BPIUUK Karangasem Bali Arief Prayoga Akbar; Pande Gde Sasmita Julyantoro; Dewa Ayu Angga Pebriani
Bumi Lestari Journal of Environment Vol 22 No 2 (2022)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/blje.2022.v22.i02.p01

Abalone is one of the sea snails that have high economic value and has advantages because it has a high protein content of 71.99%, a fat content of 3.24%, and can be used as medicine. The high demand for abalone causes the increase of wild capture and lead to scarcity of abalone in the waters. One way to overcome this problem is by cultivate the abalone. Abalone cultivation can be done with a monoculture system. One of the technologies applied is by using a recirculation aquaculture system (RAS), which is reprocessing the water used so that it can be reused for cultivation activities. The purpose of this study was to analyze the parameters of water quality, growth length, weight and survival of abalone (Haliotis squamata) seeds using the RAS system. The research was conducted in June – September 2020 at the Shrimp and Shellfish Parent Center, Karangasem, Bali. The research was performed by purposive sampling method. The water quality parameters data such as nitrite, ammonia, TSS pH, temperature, DO, and salinity were taken. The results of the measurement of water quality are nitrite 0 – 0.338 mg/L, ammonia 0 – 0.09 mg/L, TSS 0 – 15 mg/L, pH 7 – 8, temperature 26 – 28 oC, DO 4.3 – 7.07 mg/L, and salinity 31 – 38 ppt. Those values are still in the optimal range for abalone culture. The growth in length and absolute weight of abalone seeds during the study were 25.17 mm/ind. and 9.15 g/ind., respectively, and the survival value was 99.5% during the 100-day culture period.

Penghambatan Faktor Virulensi Vibrio parahaemolyticus Menggunakan Isolat Bakteri dari Saluran Pencernaan Ikan Kerapu Siti Nurlatifah; Ida Bagus Gede Darmayasa; Pande Gde Sasmita Julyantoro; Putu Eka Sudaryatma
Acta VETERINARIA Indonesiana Vol. 10 No. 3 (2022): November 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.3.228-238

Ikan kerapu adalah komoditas perikanan yang bernilai ekonomis tinggi pada pasar domestik dan internasional. Akan tetapi, dalam budidaya ikan kerapu masih sering ditemukan kasus penyakit vibrosis yang berdampak terhadap kerugian ekonomi akibat kematian yang ditimbulkan. Penyakit ini disebabkan salah satunya oleh bakteri Vibrio parahaemolyticus yang faktor virulensinya diregulasi oleh mekanisme kuorum sensing (QS). Pencegahan penyakit bakteri selama ini sering dilakukan dengan menggunakan antibiotik yang berisiko munculnya bakteri resisten antibiotik. Salah satu upaya untuk menghindari hal tersebut adalah dengan menghambat sistem QS menggunakan bakteri pendegradasi molekul sinyal N-acyl homoserine lactone (AHL). Pada penelitian ini dilakukan isolasi kandidat bakteri pendegradasi AHL pada media menggunakan AHL komersial sebagai sumber N tunggal dan juga melakukan isolasi bakteri V. parahaemolyticus pada media Thiosulfate-Citrate-BileSalts-Sucrose Agar dari saluran pencernaan ikan kerapu. Pengaruh penambahan isolat kandidat pendegradasi AHL terhadap tiga faktor virulensi V. parahaemolyticus yaitu motilitas, produksi kaseinase dan hemolisa diuji dengan mengkultur V. parahaemolyticus dengan dan tanpa penambahan isolat pendegradasi AHL. Hasil penelitian menunjukkan bahwa 2 isolat kandidat pendegradasi AHL yaitu SNA02 dan SNA03 mampu menurunkan ketiga faktor virulensi yang diuji tersebut. Hal ini mengindikasikan bahwa isolat SNA02 dan SNA03 berpotensi sebagai kandidat bakteri untuk mencegah penyakit vibriosis. Hasil ini diharapkan dapat membantu pembudidaya ikan kerapu dalam mengendalikan patogenitas bakteri V. parahaemolyticus.

APLIKASI PENGGUNAAN SISTEM KANTONG PADA BUDIDAYA RUMPUT LAUT SEBAGAI ALTERNATIF MATA PENCAHARIAN MASA PANDEMI DI PERAIRAN PANTAI KUTUH, BADUNG, BALI A.P.W.K. Dewi; I.W. Restu; P.G.S. Julyantoro; G.R.A. Kartika; N.M. Ernawati
Buletin Udayana Mengabdi Vol 20 No 3 (2021): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Permasalahan yang dihadapi oleh pembudidaya rumput laut di Pantai Pandawa saat ini adalah rumput laut tidak dapat tumbuh dengan baik. Pertumbuhan rumput laut yang tidak baik disebabkan oleh serangan hama. Solusi yang ditawarkan untuk menyelesaikan masalah tersebut adalah dengan menggunakan metode penanaman yang dimodifikasi dengan penggunaan kantong. Penggunaan kantong bertujuan untuk melindungi rumput laut dari serangan hama yang dapat mengganggu pertumbuhan rumput laut. Metode pelaksanaan yang digunakan dalam kegiatan ini adalah dengan memberikan pelatihan dan mengaplikasikan secara langsung penggunaan kantong pada budidaya rumput laut di perairan Pantai Kutuh. Kegiatan ini bekerjasama dengan pihak manajemen Pantai Pandawa yang dalam hal ini merupakan penanggung jawab Kawasan Pariwisata Pantai Pandawa. Masyarakat yang terlibat adalah sebanyak 13 orang. Kesimpulan dari kegiatan pengabdian ini adalah masyarakat menyambut baik kegiatan pengabdian dan antusias dalam mengikuti kegiatan pelatihan. Selain itu, sebanyak 43% peserta setuju untuk meneruskan budidaya rumput laut dengan metode kantong sebagai mata pencaharian.

THE EFFECT OF Bacillus sp. ADDITION ON THE VIRULENCE OF Vibrio parahemolyticus TOWARD AXENIC CULTURE OF Artemia franciscana Ni Luh Komang Ayu Maitri Jayanthi; Pande Gde Sasmita Julyantoro; Dewa Ayu Angga Pebriani
Journal of Fish Health Vol. 2 No. 1 (2022): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Research on the benefits of Bacillus sp. in aquaculture has been done a lot. Currently, the aquaculture industry, especially shrimp farming, is facing AHPND disease, which is known to be caused by Vibrio parahaemolyticus. This study aims to see the effect of Bacillus sp. against virulence of V. parahaemolyticus in vitro using caseinase production as an indicator and survival of Artemia franciscana cultured axenically when challenged with V. parahaemolyticus. In in vitro tests, cultures of V. parahaemolyticus were grown with and without the addition of Bacillus sp. on Luria Bertani (LB) liquid media, and then the caseinase test was carried out using skim milk (SM) agar as a growth medium. In the challenge test, 10 axenic cultured Artemia sp. were distributed in falcon tubes containing 10 ml of sterile seawater. Challenge test treatments consisted of treatment A as control of Artemia culture without the addition of bacteria, treatment B Artemia culture adding by 106 CFU/ml V. parahaemolyticus, treatment C Artemia culture with the addition of 106 CFU/ml Bacillus sp., and treatment D Artemia culture with the addition of 106 CFU/ml V. parahaemolyticus and 106 CFU/ml Bacillus sp. All treatments were done in triplicates. The results showed that the addition of Bacillus sp. was able to reduce the caseinase production of V. parahemolyticus up to 29% indicated by the decrease of the clearing zone diameter formed on SM agar. Furthermore, the addition of Bacillus sp. in treatment D was able to significantly increasing (P<0.05) of Artemia's survival when challenged with V. parahamolyticus. This indicates that Bacillus sp. has a potential as a probiotic candidate to prevent disease caused by V. parahaemolyticus.

Laju Pertumbuhan dan Kelulushidupan Ikan Lele Dumbo (Clarias gariepinus) dengan Padat Tebar Berbeda dalam Sistem Bioflok Dian Kharisma Ginting; Pande Gde Sasmita Julyantoro; Ni Putu Putri Wijayanti
Current Trends in Aquatic Science Vol 5 No 1 (2022)
Publisher : Program Studi Manajemen Sumberdaya Perairan, Fakultas Kelautan dan Perikanan, Universitas Udayana

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Biofloc technology is used to overcome the increase in organic matter, improve the quality of aquaculture water, and increase production yields. This study aims to determine the growth rate and survival rate of African catfish with different stocking densities in the biofloc system. This study used a completely randomized design (CRD) with 3 treatments and 5 replications. The test animals used African catfish measuring 7-9 cm with a stocking density of A (15 ind/15L), B (20 ind/15L) and C (25 ind/15L). The results showed that the use of a biofloc system in African catfish culture with different stocking densities resulted in no significant difference in growth rate, survival rate (SR) and feed conversion ratio (FCR). The average weight growth value of African catfish in treatment A, B, and C was 14,13 ± 2,26 g, 13,37±0,46 g, and 12,04±0,48 g, respectively. The average length growth in treatments A, B, and C was 6,27±0,54 cm, 6,09±0,31 cm, and 5,69±0,34 cm, respectively. The survival value (SR) of African catfish in treatment A, B, and C was 85,33±5,58%, 83±2,73%, and 82,40±2,19%, respectively. The value of the feed conversion ratio (FCR) in treatment A, B, and C was 0,72±0,08, 0,75±0,06, and 0,79±0,03, respectively. The range of values for the water quality parameters of the culture media is categorized as suitable for the growth of African catfish, resulting in temperature, DO, and pH value of 27,5-30,8°C, 3,9-5,9 mg/L, pH 6,20-6,45, respectively. The content of ammonia is 0,26-0,44 mg/L, nitrite is 0,08-0,18 mg/L and nitrate is 0,18-0,29 mg/L.

Pemberian Pelet FF-999 dengan Dosis Berbeda terhadap Pertumbuhan, Rasio Konversi Pakan dan Kelulushidupan Benih Ikan Nila (Oreochromis Niloticus) pada Sistem Batch Jefri Boy Pratama; Pande Gde Sasmita Julyantoro; Ni Putu Putri Wijayanti
Current Trends in Aquatic Science Vol 5 No 1 (2022)
Publisher : Program Studi Manajemen Sumberdaya Perairan, Fakultas Kelautan dan Perikanan, Universitas Udayana

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The purpose of this study was to determine the effect of giving FF-999 pellets with different doses on growth, feed conversion ratio (FCR), survival rate (SR) and water quality of tilapia seeds. This research was conducted at the Fisheries Laboratory, Faculty of Marine Affairs and Fisheries, Udayana University from January-February 2021. Fish samples tested were tilapia seeds (Oreochromis niloticus) obtained from UD. Mina Karya Jaya, Karangasem, Bali. Feed used in this study was FF-999 pellets. Method used is an experimental using a completely randomized design with 5 treatments and 3 repetitions. Doses of feed given in this study were in treatment A was 1%, treatment B was 2%, treatment C was 3%, treatment D was 4%, and treatment E was 5%. Results of observations for 8 weeks showed the highest absolute length and weight growth was found in treatment E, was 4.50 cm and 4.34 g. Lowest feed conversion ratio was found in treatment C was 0.81. Highest survival rate was found in treatments C and D, was 73.33%. Results of statistical analysis One-Way ANOVAwith Duncan's follow-up test showed different treatments had significant effect on absolute length and absolute weight growth, survival rate tilapia (P<0.05) and had no significant effect on feed conversion ratio tilapia (P>0, 05). Water quality parameters measured include temperature, pH, Dissolved Oxygen (DO), ammonia, nitrate, nitrite, and phosphate in all treatments was still considered feasible for growth and survival of tilapia seeds (O. niloticus).

Pendederan Ikan Nila (Oreochromis niloticus) Tahap II Menggunakan Pakan Fermentasi dengan Agen yang Berbeda Annas Cesar Mayasha; Pande Gde Sasmita Julyantoro; Ni Putu Putri Wijayanti
Current Trends in Aquatic Science Vol 5 No 2 (2022)
Publisher : Program Studi Manajemen Sumberdaya Perairan, Fakultas Kelautan dan Perikanan, Universitas Udayana

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Fermented feed application for fish nursery have been known could improve nutritional value, growth, and increase digestibility of cultured fish. The purpose of this study was to determine the effect of fermented feed with different fermentation agent on the performance of stage II tilapia nursery. The fermentation agent used in this study were fermipan containing 25 g/kg of yeast (Saccharomyces cerevisiae) and probio?7 containing 25 ml/kg of bacteria (Bacillus sp.). The study was performed by 3 treatments and 3 replications each treatment. The treatments were treatment A (control), treatment B (fermentation using S. cerevisiae 25 g/kg), and treatment C (fermentation Bacillus sp. 25 ml/kg). The observation for 2 months showed that the highest of specific growth rate of tilapia (Oreochromis niloticus) was found in treatment B with 5,12%/day, followed by treatment C (4,90%/day), and the lowest was treatment A (4,80%/day). The highest survival of tilapia (O. niloticus) was found in treatment B of 70%, followed by treatment C (63,33%), and the lowest was treatment A (60%). The results of the One-Way ANOVA statistical analysis with Duncan's post hoc test showed significantly different between treatments for the specific growth rate and survival of tilapia (P<0.05). The highest feed conversion ratio (FCR) was found in treatment A of 2,49, followed by treatment C (2,35), and the lowest was in treatment B (2,29). This FCR results were not significantly different between treatments (P>0.05). Water quality parameters measured include dissolved oxygen (DO), degree of acidity (pH), temperature, ammonia, nitrate, and nitrite were still within optimal range for Tilapia nursery culture.

Identification of Dominant Shark Fins Landed at The Port of Benoa, Bali Based on CO1 Gene Yuni Debora Prasicilia Sitorus; Pande Gde Sasmita Julyantoro; Ayu Putu Wiweka Krisna Dewi
Journal of Marine and Aquatic Sciences Vol 8 No 2 (2022)
Publisher : Fakultas Kelautan dan Perikanan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/jmas.2022.v08.i02.p07

Indonesia is one of the world's most shark-catching countries. One of the fishery ports that has a by-product of sharks is the Port of Benoa. The purpose of this study was to determine the species and phylogenetic relationship of sharks from dominant fin samples landed at the Port of Benoa, Bali based on the results of morphological identification and DNA barcoding techniques. The study was conducted from January to February 2018 at the Pengawasan Sumberdaya Kelautan dan Perikanan Base (PSDKP) Benoa. This research uses exploration method with survey technique. The data used in this research is primary data of dominant shark fin samples in research location area and secondary data of location where shark caught by fisherman based on PSDKP database. The results showed that by-catch sharks from dominant fin samples at the Port of Benoa, Bali based on morphological identification and DNA barcoding technique were Prionace glauca (Blue Shark) species. The results of phylogenetic analysis of by-catch shark species from dominant fin samples landed at the Port of Benoa, Bali have close relation with Carcharhinidae family. Prionace glauca shark species have an amplified nucleotide base length of 600 pb with similarity reaching 99 values with species present in GenBank.

Pertumbuhan dan Kelulushidupan Ikan Gabus (Channa striata) yang Diberi Pakan Maggot BSF (Hermetia illuences) I Made Angga Wahyu Dinata; Pande Gde Sasmita Julyantoro; Ni Putu Putri Wijayanti
Bumi Lestari Journal of Environment Vol 23 No 1 (2023)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/blje.2023.v23.i01.p04

Aquaculture is the fastest growing food production sector in the world. Snakehead fish (Channa striata) is one of the freshwater fish commodities in Indonesia which is still widely cultivated. Snakehead fish (Channa striata) is a fish that is economically feasible and has benefits that are beneficial to human health. This research was conducted in a period of 58 days aimed to determine the effect of feeding maggot BSF (Hermetia illuences) on the growth and survival of snakehead fish (Channa striata). The stocking density of fish in this study was 20 fish/pond, 3-4 cm in size fish obtained from cultivators in the Denpasar area, the pond used was made of tarpaulin with a size of 50×50 cm. This research used 3 treatments, treatments A (100% pellet), treatment B (100% BSF maggot), and treatment C (50% pellet + 50% BSF maggot) with 3 replications. Parameters observed in this study were specific growth rate, absolute length growth, survival rate, feed conversion ratio and water quality. The results of the research that have been carried out show that treatment C gave the highest yield on the specific growth rate, absolute length growth and survival rate of snakehead fish (Channa striata). The results of water quality measurements show that the temperature ranges from 29.32-30.18oC, pH values ??range from 6.50-5.52, and DO ranges from 2.79-2.90 mg/L, the water quality value is still in the optimum value range for maintenance of snakehead fish (Channa striata).

Co-Authors A.H.W. Sari A.P.W.K. Dewi A.P.W.K. Dewi Abrar Ravidhia Ade Angelia Atmanegara Sinaga Agus Wahyudi Alfarisi Prafanda Alfi Hermawati Waskita Alfi Hermawati Waskita Sari Alfi Hermawati Waskita Sari Alfi Hermawati Waskitasari Amayliana Ajeng Nastiti Ana Indriyanti Annas Cesar Mayasha Annisa Millenia Putri Annisa Nur Safitri Utomo Arief Prayoga Akbar Aristiani Rusdi Oktaviyanti Artanti Tri Lestari Ayu Putu Wiweka Krisna Dewi Ayu Putu Wiweka Krisna Dewi Bachori Dhian Pratama Bagus Anjasmara Bedjo Slamet Bona Rizky Simanungkalit Budhi Wahyu Nurindra Claudy Margaretha Kandouw D.A.A. Pebriani Dewa Ayu Angga Pebriani Dewa Ayu Angga Pebriani Dewa Ayu Angga Pebriani Dian Kharisma Ginting Doni Samuel Barus Endang Wulandari Endang Wulandari Suryaningtyas Endang Wulandari Suryaningtyas Errina Bening Ambartyasning Fiko Triajiatma Firda Nurdiana G.R.A. Kartika G.R.A. Kartika Galang Alamin Trisnabatin Gde Raka Angga Kartika Grace Adelina Girsang Handayani Sitorus Hasbia Rahmat Hasrat Damai Hulu I Bagus Andreana Surya Nugraha I Gede Yoga Vikannada Giri I Gusti Ayu Agung Purnama Sari I Gusti Ayu Febiana Putri Wardeni I Gusti Ngurah Permana I Kadek Alamsta Suarjuniarta I Kadek Mega Mega Dhyana Putra I Ketut Wija Negara I Ketut Wija Negara I Komang Yopi Trio Santana I Made Angga Wahyu Dinata I Made Budiarsana I Made Hendy Wesha Suryawan I Wayan Kasa I Wayan Restu I.K.W. Negara I.W. Arthana I.W. Restu Ida Ayu Grisandi Dewi Kurnia Ida Bagus Gede Darmayasa Ilham Muttaqin Jefri Boy Pratama John Lynham Jovita Larasati Poetri Tajuw Kadek Bayu Candra Gotama Kartika, I Wayan Darya Luh Gede Manik Radzena Martha M Reza Mei Budi Dharmawan Made Ayu Pratiwi Made Ayu Pratiwi Maria Ulfa Maulana Ilham Pratama N.M. Ernawati N.M. Ernawati N.P.P. Wijayanti Ni Desak Putu Ida Suryani Ni Kadek Vindi Wedhawati Ni Luh Gede Rai Ayu Saraswati Ni Luh Komang Ayu Maitri Jayanthi Ni Luh Putu Ayu Puspita Dewi Ni Made Ernawati, Ni Made Ni Made Putri Handayani Ni Nyoman Krisna Sanidhi Putri Ni Putu Emie Noviana Ni Putu Putri Wijayanti Ni Putu Putri Wijayanti Ni Putu Sandhitya Candra Kartina Ni Putu Tika Lestari Ni Putu Widiantari Nu'man Najib Putu Bagaskara Putu Eka Sudaryatma Putu Eka Sudaryatma Putu Niken Ayu Saraswati Rai Ayu Ratri Chandra Pandunita Rani Ekawaty Ratriana Hartini Saleh S.A. Saraswati Salman Mokoginta Shanti Paramita Jayanti Siti Nurlatifah Sukarman Sukarman Suprabadevi Ayumayasari Saraswati Suprabadevi Ayumayasari Saraswati Syifa Maria DiazGonzales Wahyu Ilvita Vindia Wahyu Nurlita Widiastuti Widiastuti Widya Purnama Dewi Yan Ramona Yopie Ryan Audy Harahap Yova Tresya Galingging Yufinta Cahya Permanti Yuni Debora Prasicilia Sitorus

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