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Distribusi Mikrohabitat dan Infeksi Endoparasit pada Ikan Kembung (Rastrelliger sp.) yang didaratkan di PPI Kedonganan, Badung, Bali Claudy Margaretha Kandouw; Pande Gde Sasmita Julyantoro; Dewa Ayu Angga Pebriani
Journal of Marine and Aquatic Sciences Vol 9 No 1 (2023)
Publisher : Fakultas Kelautan dan Perikanan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/jmas.2023.v09.i01.p13

Abstract

Mackerel (Rastrelliger sp.) is a pelagic fish commodity that has high economic value because mackerel is a consumption fish and is easily found in all sea waters in Indonesia. Mackerel is a fish that is hunted for consumption because of its high omega-3 content, so it is really necessary for us to pay attention to the quality of the mackerel from the threat of parasites that can cause disease. This research was being conducted to determine the distribution of endoparasite microhabitats, intensity, prevalence, and the relation between the length of the fish and weight of fish with the number of parasites. The research samples were collected from Kedonganan Fish Landing Base, Badung, Bali, which was carried out from October 2021 to November 2021. The research used a descriptive quantitative method and the sampling method was carried out by random sampling. The total samples of fish examined were 35 fish consisting of two species, R. Kanagurta (20 fish) and R. brachysoma (15 fish). Endoparasites of the Anisakis genus were parasites with the highest intensity and prevalence values : 4,06 ind/fish and 82,86% with a low infection rate. The relation between the length of the fish and body weight of fish with the number of parasites was in the opposite direction and had a very low effect with a value of correlation (r) between length and the number of parasites are 0,031 and a value of correlation (r) between weight and number of parasites are 0,00547.
Optimasi Suhu terhadap Pertumbuhan dan Kelulushidupan Ikan Heckel Discus (Symphysodon discus) Fiko Triajiatma; Pande Gde Sasmita Julyantoro; Alfi Hermawati Waskitasari
Current Trends in Aquatic Science Vol 6 No 2 (2023)
Publisher : Program Studi Manajemen Sumberdaya Perairan, Fakultas Kelautan dan Perikanan, Universitas Udayana

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Abstract

This study aims to determine the optimization of temperature on the growth and survival of discus fish. The treatments used were different temperatures at 25°C, 28°C, and 31°C. Discus fish maintenance is carried out in 3 aquariums with a size of 120 × 40 × 40 cm which have been given a filter, aeration and heater. The water used is 120 liters, which has been aerated for 24 hours with a predetermined temperature using a heater (50W) and a water change of about 25% every week. Feeding is done by the satiation method, given 3 times a day with frozen worms where after 1 hour of feeding will be done to clean the remaining feed. Sampling of water quality (pH) using manual sampling method as a supporting parameter. The test fish used in this study were 5 cm discus fish. The number of fish used was 27 fish, and measurements were taken every 15 days with 3 repetitions. Length and weight growth of discus fish increased during the study. The growth of discus fish (Symphysodon sp.) treated at 31°C was the most optimum growth with absolute length reaching 1,56 cm and absolute weight of 3,55 g compared to the 25°C and 28°C temperature treatments. The survival rate of discus fish in the 25° C, 28° C, and 31° C temperature treatments has a survival rate value of 100% and water quality that remains stable due to optimum temperature vulnerability.
Pengaruh Penambahan Senyawa Thiophenone terhadap Kelulushidupan dan Pertumbuhan Larva udang galah yang Diujitantang dengan Bakteri Vibrio harveyi Maulana Ilham Pratama; Pande Gde Sasmita Julyantoro; I Ketut Wija Negara
Current Trends in Aquatic Science Vol 6 No 1 (2023)
Publisher : Program Studi Manajemen Sumberdaya Perairan, Fakultas Kelautan dan Perikanan, Universitas Udayana

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Abstract

Giant prawns are one of the freshwater aquaculture commodities with high economic value. The high demand for giant prawns is constrained by the low quality of larvae, which affect the prawn growth to reach consumption sizes. Conventional efforts have been made to treat the bacterial diseases in the prawn cultivation using antibiotics. However, it has risk of causing pathogenic bacteria to become resistant to antibiotics. Such issue can be overcome by using inhibiting quorum sensing from pathogenic bacteria, especially thiophenone compounds. This study aimed to determine the effect of adding thiophenone compounds on the survival and growth of giant prawn larvae from Center for Giant Prawns (BBUG) Klungkung, Bali tested with Vibrio harveyi. A completely randomized design of 4 treatments (A: control, B: Larvae and Vibrio harveyi (105 CFU/ml), C: Larvae and thiophenone (10µM), D: Larvae, thiophenone (10µM) and Vibrio harveyi (105 CFU/ml)) and 3 repetitions was done in laboratory. A total of 12 experimental units containing 20 larvae with 0.2liter seawater media at 28?C. The results showed that the survival rate of giant prawn larvae was significantly higher when the larvae challenged with Vibrio harveyi compared to un challenged larvae. Furthermore, there was no difference in prawn larvae growth between the treatments indicated by similar value of Larvae Stage Index (LSI). This study has demonstrated the benefit of employing thiophenone compounds to improve the quality of giant prawn larvae. Further study in challenging the thiophenone compounds with other bacteria that might threat the giant prawn growth is still needed.
Pengaruh Penambahan Saccharomyces cerevisiae pada Pakan terhadap Pertumbuhan Ikan Kakap Putih (Lates calcarifer) I Made Budiarsana; Pande Gde Sasmita Julyantoro; Alfi Hermawati Waskita Sari; I Gusti Ngurah Permana
Current Trends in Aquatic Science Vol 6 No 1 (2023)
Publisher : Program Studi Manajemen Sumberdaya Perairan, Fakultas Kelautan dan Perikanan, Universitas Udayana

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Abstract

White snapper (Lates calcarifer) is one of the marine aquaculture commodities in Indonesia, which has high economic value and fast growth. This study aims to investigate the influence of Saccharomyces cerevisiae addition into feed on the growth of white snapper (Lates calcarifer). The growth observed was the absolute length growth, the absolute weight, feed conversion ratio, survival, and specific growth rate. This research used experimental methods with complete randomized design non-factorials. White snapper (size 2-3 cm) was maintained for 30 days at a stocking density of 1500 Ind/m3. There were 2 treatments conducted, K treatment (control) and S treatment (pellet + Saccharomyces cerevisiae 100 ml/kg feed). Data analysis was done using t-test. The result showed that the addition of Saccharomyces cerevisiae resulted in decreasing of feed conversion ratio. The absolute length and weight, specific growth rate and survival were higher than the treatment without the addition of Saccharomyces cerevisiae, but not statistically different (p>0,05). The S treatment resulted in higher value of the feed conversion ratio (2,28), growth (7,21 cm/month), absolute weight growth (13,75 g/ month), the specific growth rate (11,92 % /day) and survival of (84,07 % /month). Water quality values such as temperature ranged from 29-31,6 OC salinity 27-32 ppt, pH 7,11-7,57, and dissolved oxygen 1,74-5,64 ppm.
Intensitas dan Prevalensi Parasit Pada Insang Ikan Nila yang dibudidayakan di Balai Benih Ikan (BBI) Sentral Sangeh I Gusti Ayu Agung Purnama Sari; Pande Gde Sasmita Julyantoro; Dewa Ayu Angga Pebriani; Endang Wulandari Suryaningtyas
Current Trends in Aquatic Science Vol 6 No 2 (2023)
Publisher : Program Studi Manajemen Sumberdaya Perairan, Fakultas Kelautan dan Perikanan, Universitas Udayana

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Abstract

Parasites in the body of cultivated fish will cause harm to the fish, because the parasites take nutrients from their hosts, it is necessary to study the intensity and prevalence of parasites in tilapia (Oreochomis niloticus) in order to determine the density of parasite attacks and the percentage of parasites in tilapia. Samples of tilapia (Oreochomis niloticus) were taken from the Central Sangeh Fish Seed Center (BBI). The research was conducted for 3 months from October 4th 2022 to December 13 2022 randomly sampling. Samples of tilapia (Oreochomis niloticus) examined were 36 samples of Nila Nirwana fish. The parasites found in the external organs (ectoparasites) were only found in the tilapia gills, while for the internal organs (endoparasites) parasites were not found in both the stomach and intestines of the fish. The ectoparasites found were Dactylogyrus sp. (2181 ind) and Gyrodactylus sp. (157 ind). The highest intensity was in the genus Dactylogyrus of 60.6 ind/fish including the severe category, while for the genus Gyrodactylus of 4.4 ind/head including the low category. The highest prevalence value was in the genus Dactylogyrus with 92% in the always category while in the genus Gyrodactylus it was 78% in the usual category. This study’s supporting data for water quality includes temperature, pH, DO, and ammonia. Where temperature during the study ranged from 28-30oC, pH or acidity ranged from 7.6-8.5, and DO or dissolved oxygen ranged from 7.3-8.4 mg/L. The water quality result in this study were normal for fish farming.
Hubungan Postur Ikan dengan Jumlah Endoparasit pada Ikan Tongkol (Auxis rochei) yang Didaratkan di Pantai Kedonganan, Bali Annisa Millenia Putri; Pande Gde Sasmita Julyantoro; Dewa Ayu Angga Pebriani
Current Trends in Aquatic Science Vol 6 No 1 (2023)
Publisher : Program Studi Manajemen Sumberdaya Perairan, Fakultas Kelautan dan Perikanan, Universitas Udayana

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Abstract

This study aims to determine the various types of parasites that infect tuna, their abundance, prevalence, level of parasite intensity and the effect of fish length on the number of parasitic infections. Fish samples were obtained from Kedonganan Beach, Bali from November-December 2021. This study used a descriptive method and data collection using simple random sampling. The total samples examined were 35 fish. The endoparasites found in the internal organs of fish which include the intestines, liver, and gonads consist of 3 different genera, namely: Rhadinorhynchus sp., Anisakis sp., and Lecithocladium sp. The highest abundance was found in the genus Rhadinorhynchus sp., the highest prevalence was found in the genus Rhadinorhynchus sp., and the highest intensity level was found in the genus Lecithocladium sp. The relationship between length and number of parasites was classified as "very weak" because the correlation between the two relationships ranged from 0.00-0.199. So, it can be concluded from the research that has been done related to the relationship between fish posture and the number of endoparasites that infect the tuna (Auxis rochei) which was landed on the beach of Kedonganan, Bali. There is no relationship between fish body posture and the number of endoparasite infections or can be classified into the category of a "very weak" relationship.
Pengaruh Pemberian Pakan Pelet dan Artemia sp. terhadap Kualitas Warna Ikan Badut (Amphiprion ocellaris) Rai Ayu Ratri Chandra Pandunita; Pande Gde Sasmita Julyantoro; Ni Putu Putri Wijayanti
Current Trends in Aquatic Science Vol 6 No 1 (2023)
Publisher : Program Studi Manajemen Sumberdaya Perairan, Fakultas Kelautan dan Perikanan, Universitas Udayana

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Abstract

This study aims to determine the effect of different feeding on the color quality and growth of clown fish. The feed used in the form of pellets nd Artemia sp. with three treatments and three repetitions. The treatments used were pellet feed (treatment A), Artemia sp feed. (treatment B), and the combination feed of pellet feed and Artemia sp. (treatment C). The research method used is experimental method using Completely Randomized Design (CRD). This research was conducted at PT Agung Aquatic Marine for 1 month October until November. This study used 18 clown fish fed 3 times a day with a dose of 1.5 g of Fengli pellets and 1.5 ml of Artemia sp. for one administration. The parameters measured in this study was color quality parameters. Parameter measurements were carried out once a week. The media used was a aquarium with a volume of 2500 ml with a size of 40 × 30 cm. Color quality parameters are measured with a color chart paper base called M-TCF and then ranking according to the numbers on the paper. The results of this study showed that the quality of the body color of clown fish fed pellet feed, Artemia sp. feed, and combination feed faded. Based on the results of the research that was tested by ANOVA analysis, it can be concluded that this different feeding had no significant effect (P>0.05) on the color quality of clown fish.
Korelasi Jenis Kelamin Ikan dengan Jumlah Endoparasit pada Ikan Layur (Trichiurus lepturus) yang diperdagangkan di Pasar Ikan Kedonganan, Bali Ni Luh Putu Ayu Puspita Dewi; Pande Gde Sasmita Julyantoro; Ni Putu Putri Wijayanti; Endang Wulandari Suryaningtyas
Bumi Lestari Journal of Environment Vol 23 No 2 (2023)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/blje.2023.v23.i02.p10

Abstract

Swordfish (Trichiurus lepturus) with the local name Layur is one of the demersal fish commodities that has high economic value because it is an export commodity with high omega 3 content.. The high demand for layur fish must be supported by good fish quality and quality. The quality control is needed related to fish diseases, one of which is fish parasites. Endoparasite infection in marine fish can be zoonotic and dangerous if consumed by humans. This research was conducted to determine the intensity, prevalence, abundance and correlation of fish sex with the number of endoparasites. The research sample was obtained from the Kedonganan Fish Market, Bali which was held from September 2022 to November 2022. The sampling method was carried out by purposive sampling by paying attention to the secondary sexual characteristics of the swordfish. The total sample of fish examined was 36 fish consisting of 18 male fish and 18 female fish. Endoparasites of the genus Acanthobothrium were parasites with the highest intensity and abundance values, namely 46.05 ind./fish and 28.14 ind./fish, while the highest prevalence value was obtained from the Genus Lecithochirium of 86.11%. The correlation between fish sex and the number of endoparasites was analyzed using the Mann – Whitney test with an Asymp value. Sig 0.506 and Z value -0.665. These results showed an insignificant relationship (p > 0.05) between the sex of the fish and the number of endoparasites in the whitefish that were sampled in this study. Keywords: Endoparasites; Gender; Number of Parasites; Trichiurus lepturus
Pemanfaatan Langsung Tanaman Kayu Apu Dadak (Azolla pinnata) Sebagai Pakan Tambahan pada Budidaya Ikan Nila (Oreochromis niloticus) Ni Putu Sandhitya Candra Kartina; Pande Gde Sasmita Julyantoro; Ni Putu Putri Wijayanti
Bumi Lestari Journal of Environment Vol 23 No 2 (2023)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/blje.2023.v23.i02.p07

Abstract

Tilapia is a type of fish favored by freshwater fish consumers because it is easy to cultivate. The problem faced in freshwater fish farming activities is the high price of artificial feed. To deal with these problems, alternative feeds are needed to reduce the use of artificial feeds such as sudden apu wood (Azolla pinnata). This study aims to determine the effectiveness of the sudden apu wood (Azolla pinnata) on the growth and survival of tilapia (Oreochromis niloticus) and to help fish farmers reduce the use of artificial feed. The research method used is an experimental approach method. The experimental design in this study was a Randomized Block Design (RAK) which consisted of 3 treatments with 3 replications. Treatment A (direct feeding of 100% Azolla pinnata plants), Treatment B (direct feeding of 50% Azolla pinnata plants and 50% pellets), and Treatment C (100% pellet feeding). The results showed the absolute weight growth of treatment A 12.18±5.66 g, treatment B 17.73±4.40 g, treatment C 24.53±3.80 g. The absolute length growth of treatment A was 2.22 ± 0.60 cm, treatment B 2.54 ± 0.40 cm, treatment C 3.50 ± 0.37 cm. The survival rate of treatment A was 66.67±11.54%, treatment B and treatment C were 100±0%. Feed conversion ratio (FCR) for treatment A 4.8±2.28, treatment B 2.1±0.45, treatment C 1.7±0.17. The water quality of the three feeding treatments had no significant effect on pond water quality and was still within the optimal range for tilapia growth and cultivation.
Role of Catecholamine Hormone to Increase the Virulence of Aeromonas hydrophila Ni Putu Widiantari; Yan Ramona; Pande Gde Sasmita Julyantoro
Metamorfosa: Journal of Biological Sciences Vol 10 No 2 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2023.v10.i02.p17

Abstract

Lingkungan yang ekstrim telah banyak dilaporkan dapat memicu terjadinya stres pada mahluk hidup yang terpapar, sehingga mengganggu kondisi homeostasinya. Dalam keadaan tertekan, organisme vertebrata akan melepas hormon-hormon stres, seperti katekolamin Norepinephrin (NE) dan Dopamin (Dopa), yang melibatkan axis hypothalamus-sympathetic-chromaffin (HSC) dan axis hypothalamus–pituitary-interrenal (HPI). Kedua sistem axis tersebut berturut-turut menghasilkan hormone katekolamin (antara lain adrenalin, noradrenalin, dan dopamine) dan hormon kortikosteroid (terutama kortisol). Kehadiran kedua kelompok hormon tersebut pada sistem peredaran darah inang berperan sebagai signal bagi bakteri patogen, seperti Aeromonas hydrophila, yang umum ditemukan dalam akuakultur, untuk memulai proses infeksinya. Hormon berperan untuk mengaktifkan gen-gen dalam aktivitas dan pembentukan flagella (memungkinkan sel bakteri berenang dengan cepat menuju inangnya), pembentukan pilli, produksi toksin, dan mengaktivasi proses pembentukan biofilm. Dengan kata lain, kondisi stres pada inang ini mempengaruhi metabolisme, pertumbuhan dan virulensi bakteri patogen tersebut, karena kondisi sistem imun inang berada pada titik terendah ketika organisme tersebut mengalami stres. Sekresi hormone katekolamin juga merupakan signal bagi bakteri patogen untuk mengaktifkan gen ferric uptake regulator (fur gene) yang berperan dalam proses pengkhelatan zat besi (Fe3+) di lingkunganya, sehingga terjadi pertumbuhan berlebih dari patogen tersebut. Peran hormone katekolamin dalam peningkatan patogenitas patogen adalah melemahkan ikatan kompleks protein transferrin dengan zat besi (Fe3+), sehingga terjadi peningkatan laju pertumbuhan patogen yang akhirnya berdampak pada peningkatan patogenitasnya. Kata kunci : Akuakultur, Endokrinologi mikroba, Patogenisitas, Stres.
Co-Authors A.H.W. Sari A.P.W.K. Dewi A.P.W.K. Dewi Abrar Ravidhia Ade Angelia Atmanegara Sinaga Agus Wahyudi Alfarisi Prafanda Alfi Hermawati Waskita Alfi Hermawati Waskita Sari Alfi Hermawati Waskita Sari Alfi Hermawati Waskitasari Amayliana Ajeng Nastiti Ana Indriyanti Annas Cesar Mayasha Annisa Millenia Putri Annisa Nur Safitri Utomo Arief Prayoga Akbar Aristiani Rusdi Oktaviyanti Artanti Tri Lestari Ayu Putu Wiweka Krisna Dewi Ayu Putu Wiweka Krisna Dewi Bachori Dhian Pratama Bagus Anjasmara Bedjo Slamet Bona Rizky Simanungkalit Budhi Wahyu Nurindra Claudy Margaretha Kandouw D.A.A. Pebriani Dewa Ayu Angga Pebriani Dewa Ayu Angga Pebriani Dewa Ayu Angga Pebriani Dian Kharisma Ginting Doni Samuel Barus Endang Wulandari Endang Wulandari Suryaningtyas Endang Wulandari Suryaningtyas Errina Bening Ambartyasning Fiko Triajiatma Firda Nurdiana G.R.A. Kartika G.R.A. Kartika Galang Alamin Trisnabatin Gde Raka Angga Kartika Grace Adelina Girsang Handayani Sitorus Hasbia Rahmat Hasrat Damai Hulu I Bagus Andreana Surya Nugraha I Gede Yoga Vikannada Giri I Gusti Ayu Agung Purnama Sari I Gusti Ayu Febiana Putri Wardeni I Gusti Ngurah Permana I Kadek Alamsta Suarjuniarta I Kadek Mega Mega Dhyana Putra I Ketut Wija Negara I Ketut Wija Negara I Komang Yopi Trio Santana I Made Angga Wahyu Dinata I Made Budiarsana I Made Hendy Wesha Suryawan I Wayan Kasa I Wayan Restu I.K.W. Negara I.W. Arthana I.W. Restu Ida Ayu Grisandi Dewi Kurnia Ida Bagus Gede Darmayasa Ilham Muttaqin Jefri Boy Pratama John Lynham Jovita Larasati Poetri Tajuw Kadek Bayu Candra Gotama Kartika, I Wayan Darya Luh Gede Manik Radzena Martha M Reza Mei Budi Dharmawan Made Ayu Pratiwi Made Ayu Pratiwi Maria Ulfa Maulana Ilham Pratama N.M. Ernawati N.M. Ernawati N.P.P. Wijayanti Ni Desak Putu Ida Suryani Ni Kadek Vindi Wedhawati Ni Luh Gede Rai Ayu Saraswati Ni Luh Komang Ayu Maitri Jayanthi Ni Luh Putu Ayu Puspita Dewi Ni Made Ernawati, Ni Made Ni Made Putri Handayani Ni Nyoman Krisna Sanidhi Putri Ni Putu Emie Noviana Ni Putu Putri Wijayanti Ni Putu Putri Wijayanti Ni Putu Sandhitya Candra Kartina Ni Putu Tika Lestari Ni Putu Widiantari Nu'man Najib Putu Bagaskara Putu Eka Sudaryatma Putu Eka Sudaryatma Putu Niken Ayu Saraswati Rai Ayu Ratri Chandra Pandunita Rani Ekawaty Ratriana Hartini Saleh S.A. Saraswati Salman Mokoginta Shanti Paramita Jayanti Siti Nurlatifah Sukarman Sukarman Suprabadevi Ayumayasari Saraswati Suprabadevi Ayumayasari Saraswati Syifa Maria DiazGonzales Wahyu Ilvita Vindia Wahyu Nurlita Widiastuti Widiastuti Widya Purnama Dewi Yan Ramona Yopie Ryan Audy Harahap Yova Tresya Galingging Yufinta Cahya Permanti Yuni Debora Prasicilia Sitorus