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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
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Characterization of Carambola (Averrhoa carambola L.) Plant Collection of Cibinong Plant Germplasm Garden Based on Phenotypic and Genetic Characters Priadi, Dody; Perdani, Ambar Yuswi; Sulistyowati, Yuli; Pohan, Fiqolbi Nuro; Mulyaningsih, Enung Sri
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5199

Abstract

Indonesia as a rich biodiversity country has many superior fruit plant germplasms such as sweet star fruit or carambola (Averrhoa carambola L.). Some varieties of carambola which collected at the Germplasm Garden of Research Center for Biotechnology-LIPI have been used for parent trees of fruit plant production. Therefore, they have to be characterized both phenotypically and genetically. The objective of the study was to analyze the relationship between eight varieties of carambola i.e. Malaysia, Penang, Rawasari, Bangkok, Sembiring, Dewabaru, Demak and Dewimurni at the germplasm garden based on phenotypic and genetic characters. Phenotypic characters were observed directly in the field, whereas genetic characters were observed with RAPD markers using 10 primers. Phylogenetic analysis was done using NT-SYS software showed that there were three clusters of carambola varieties. Meanwhile, Malaysia and Penang varieties have closed relationships (96%) compared with the other varieties. The result of the study would be dedicated to updating and completing the existing fruit plant collection database of Plants Germplasm Garden.
Kelimpahan Serangga Predator kutudaun Aphis gossypii di Sentra Tanaman Sayuran di Sumatera Selatan -, Khodijah
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 2 (2014): September 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i2.3100

Abstract

Aphis gossypii sebagai vektor penyakit virus keriting yang dapat menyebabkan kerugian hingga 35% sebagai hama sebagai vektor dapat menyebabkan kerugian hingga 90%. Tujuan penelitian untuk mengeksplorasi dan mengidentifikasi serangga predator yang ditemukan di Sumatera Selatan. Survei serangga predator dilakukan pada tanaman cabai, tomat, dan mentimun. Hasil survei di Sumatera Selatan, yaitu daerah Sukarami, Inderalaya, Tanjung Raja, Gelumbang, Kenten, dan Talang Buruk telah ditemukan dua belas (12) spesies serangga predator A. gossypii yang yaitu, 10 spesies dari famili Coccinellidae, dan satu spesies dari famili Syrphidae dan Chamaemyiidae. Dua belas (12) spesies serangga predator yang ditemukan adalah Coccinella arcuata Fabricius, Coccinella repanda Thunberg, Menochilus sexmaculatus (Fabricius), Coelophora maculata Thunberg, Coelophora pupillata Swartz, Verania lineata Thunberg, Verania discolor Fabricius, Chilocorus ruber Weise, Coelophora reniplagiata Mulsant, Chilocorus sp., Ischidion sp., dan spesies yang tidak dapat diidentifikasikan Chamaemyiidae. Yang paling dominan dari spesies serangga predator ditemukan adalah M. sexmaculatus. Kelimpahan tertinggi dari predator ditemukan pada cabai.Aphis gossypii as the vector of mosaic virus disease have caused loss of up to 35% as pest and up to 90% as vector. The aim of the research was to explore and identify the predatory insects found in vegetable plants around South Sumatera. The survey was carried out on chilies, tomatoes and cucumbers. Result showed that in South Sumatera, particularly in regions of Sukarami, Inderalaya, Tanjung Raja, Gelumbang, Kenten, and Talang Buruk it have been found twelve (12) species of predatory insects against A. gossypii, in which 10 species were from Coccinellidae family, and one species each were from Syrphidae and Chamaemyiidae families.. The twelve (12) species were Coccinella arcuata Fabricius, Coccinella repanda Thunberg, Menochilus sexmaculatus (Fabricius), Coelophora maculata Thunberg, Coelophora pupillata Swartz, Verania lineata Thunberg, Verania discolor Fabricius, Chilocorus ruber Weise, Coelophora reniplagiata Mulsant, Chilocorus sp., Ischidion sp., and one unidentified species from Chamaemyiidae family. The most dominant species was M. sexmaculatus. The highest abudance of predators was found in chilies.
The Effect of Physical Activity agains the Telomere Length in the Leukocytes Cells of KONI Athletes Purwaningsih, Endang; Djannatun, Titiek; Widayanti, Etty; Suciati, Yulia; Zulhamidah, Yenni
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.6207

Abstract

Telomeres are strands of non coding DNA at the ends of chromosomes that have the primary function to protect DNA from damage and maintain chromosomal stability. Physical exercise will increase the antioxidant activity can increase telomere proteins, lengthen telomeres and or protein networks associated with telomere so that the telomere remains long, or stopping telomere shortening. Telomere length was also associated with age. The purpose of the research was to determine telomere length of leukocyte cells in the KONI (Indonesian National Sports Committee) athletes in Jakarta. The research method is descriptive, by measuring telomere length using quantitative PCR on leukocyte cells. Samples are KONI athletes from several sports, including men and women athletes, with ages between 15-20 years. Used a control group (not athletes) is students of the Faculty of Medicine, University of YARSI. The results showed that there was no significant difference (p> 0.05) between telomere length group of athletes with the control group in both sexes. Similarly, telomere length between athlete male with female athletes also showed no significant difference (p> 0.05). It was concluded that physical exercise in athletes KONI at the age of 15- 20 years had no effect on telomere length in leukocytes. The results of this study provide information about the telomere length in Indonesian athletes at an early age.
Characterization of Rambutan Cultivars (Nephelium lappaceum) Based on Leaf Morphological and Genetic Markers Manggabarani, Andi Madihah; Chikmawati, Tatik; Hartana, Alex
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.12221

Abstract

Rambutan (Nephelium lappaceum) is an economically important plant which is native to Indonesia and Malaysia. The diversity of rambutan in Indonesia is abundance especially in Kalimantan where the wild relatives still grow naturally. Rambutan cultivars are usually differed from each other based on fruit morphological characters. However, rambutan tree begins to fruiting for the first time in 3-4 years. Therefore, another character is needed to characterize each cultivar in a short period. The objectives of this study were to distinguish rambutan cultivars using leaf morphological and Inter-Simple Sequence Repeat (ISSR). As many as 30 rambutan cultivars collected from Cipaku Orchard and Mekar Sari Park were observed for their morphological and ISSR characters. Six characters of were surveyed for leaf morphological character. For the genetic character, 6 out of 31 ISSR primers were assessed which resulted in 58 polymorphic bands (87%). As a result, leaf morphological characters overlapped among cultivars causing difficulties distinguishing each cultivar. ISSR marker, three major clusters have been identified according to UPGMA method. Index similarity among rambutan accessions from ISSR data ranged from 48-93%. As a conclusion, ISSR marker could be potentially applied rambutan cultivars characterization.
Efek Antibakteri Ekstrak Daun Mimba (Azadirachta indica A. Juss) terhadap Bakteri Vibrio algynoliticus Secara In Vitro Ayini, Uli; B., Siti Harnina; Dewi, Titis Candra
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 1 (2014): March 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i1.3787

Abstract

Budidaya udang windu di Indonesia telah berkembang pesat. Salah satu kendala budidaya udang adalah penyakit Vibriosis yang disebabkan oleh bakteri Vibrio algynoliticus. Tujuan penelitian ini adalah untuk mengetahui efek antibakeri ekstrak daun mimba terhadap bakteri Vibrio algynoliticus. Penelitian ini menggunakan metode dilusi untuk mengetahui efek antibakteri ekstrak daun mimba terhadap bakteri Vibrio algynoliticus secara in vitro. Konsentrasi ekstrak yang digunakan (%) yaitu: 0; 2,5; 5; 7,5; 10; 12,5 dan sebagai kontrol terdiri dari kontrol positif, dan kontrol negatif. Pengumpulan data untuk menentukan MIC (Minimum Inhibitory Concentration) dilakukan dengan membandingkan kejernihan kultur di medium TSB 2% pada berbagai konsentrasi yang berbeda, dengan kontrol positif dan kontrol negatif. Penentuan MBC (Minimum Bacterisidal Concentration) dilakukan dengan melihat ada tidaknya dan jumlah koloni bakteri Vibrio alginolyticus yang muncul pada medium subkultur TSA 2% setelah inkubasi 24 jam. Hasil penelitian menunjukkan nilai MIC yaitu konsentrasi 5%, hal ini ditunjukkan dengan tabung yang mulai jernih. Nilai MBC ekstrak daun mimba terhadap bakteri Vibrio alginolyticus adalah konsentrasi 12,5% ditandai dengan sudah tidak munculnya koloni bakteri Vibrio alginolyticus. Berdasarkan penelitian ini dapat disimpulkan bahwa ekstrak daun mimba dapat memberikan efek antibakteri terhadap bakteri Vibrio alginolyticus secara in vitro.Tiger shrimp cultivation in Indonesia has been growing rapidly. The main obstacle is the shrimp farming vibriosis disease caused by the bacterium Vibrio algynoliticus. The aim of this research was to determine the effects of neem leaf extract antibakeri against Vibrio algynoliticus. This study used a dilution method to determine the antibacterial effect of neem leaf extract against Vibrio algynoliticus bacteria in vitro. The concentration of the extract used (%): 0; 2.5; 5; 7.5; 10; 12.5 and as a control consisting of a positive control, and negative control. Data collection to determine the MIC (Minimum Inhibitory Concentration) was done by comparing the clarity of culture in TSB medium 2% on a variety of different concentrations, the positive control and a negative control. Determination MBC (Minimum Bacterisidal Concentration) was done by looking at the presence or absence and the number of colonies of bacteria Vibrio alginolyticus that appears in the subculture medium TSA 2% after 24 h incubation. The results showed that the value of MIC was 5%, as shown by the tube began to clear. Value of MBC of neem leaf extract against Vibrio alginolyticus was characterized by a concentration of 12.5% has been no emergence of bacterial colonies Vibrio alginolyticus. Based on the research, it concluded that neem leaf extract can provide antibacterial effect against bacteria Vibrio alginolyticus in vitro.
EFEKTIVITAS PEMBERIAN MINYAK SAWIT DAN MINYAK LEMURU DALAM MEMPERCEPAT PUBERTAS TIKUS BETINA Na’ima, Mirtaati; Susanti, R.; Christijanti, Wulan
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 1 (2012): March 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i1.2266

Abstract

Penelitian ini bertujuan untuk mengkaji efektivitas pemberian minyak sawit dan minyak lemuru dalam mempercepat pubertas tikus betina, pada parameter peningkatan berat badan, berat ovarium dan perkembangan folikel ovarium. Tikus betina usia 21 hari sebanyak 20 ekor dibagi menjadi 4 kelompok variasi pemberian minyak sawit dan minyak lemuru per oral, yaitu kelompok A (0%+0%), B (3%+3%), C (4%+4%), dan D (5%+5%). Pada akhir penelitian (hari ke-20), dilakukan pengambilan data peningkatan berat badan, berat ovarium, dan perkembangan folikel ovarium. Data dianalisis secara deskripsi dan statistik dengan anava satu arah. Jika terdapat perbedaan, dilanjutkan dengan uji Beda Nyata Terkecil. Hasil penelitian ini menunjukkan bahwa minyak sawit dan minyak lemuru dapat mempercepat pubertas tikus betina khususnya dari peningkatan berat badan, berat ovarium, dan perkembangan folikel ovarium. Dosis paling efektif untuk mempercepat pubertas adalah 4% minyak sawit + 4% minyak lemuru. This study was aimed to examine the effectiveness of palm and lemuru oil for the acceleration of female rat puberty, especially in increasing the body weight, the ovarian weight and the follicle development. Twenty female rats aged 21 days were divided into 4 based on the variation of orally feeding palm and lemuru oil, namely group A (0% +0%), B (3% +3%), C (4% +4%), and D (5% +5%). At the twentieth day, data of the increase of the body weight, the ovarian weight and the follicle development were collected and then analyzed descriptively and statistically using one-way ANOVA. For any difference, the Least Significance Difference Test would be performed. The result of this study showed that palm and lemuru oil can accelerate the puberty of female rats. The most effective dose was 4% palm oil and 4% lemuru oil.
Effect of Carica papaya Leaf Juice on Hematology of Mice (Mus musculus) with Anemia Hamidah, Afreni; Anggereini, Evita; Nurjanah, Nurjanah
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11427

Abstract

Anemia is defined as a decrease in haemoglobin concentration and the number of erythrocytes. One source of iron in vegetable is the papaya leaf. This study aimed to determine the number of erythrocytes and hemoglobin levels after the application of Carica papaya leaf on mice through the sodium nitrite induction. The research design used was Randomized Design Complete by using five treatments with five repetitions on each treatment. The data measured in this study was number of erythrocytes and hemoglobin levels of rats before being given sodium nitrite, after being given sodium nitrite and after being given papaya leaf juice. The data obtained were analyzed statistically by using variance analysis (ANOVA), and the data that significantly different was then analyzed by Duncan Multiple Range Test (DMRT). The results showed that papaya leaf juice significantly affected the increase of erythrocyte number and hemoglobin level. The concentration that gives the best effect in increasing the number of erythrocytes is 50% papaya leaf juice concentration and 25% papaya leaf juice concentration which gives the best effect in increasing the hemoglobin content. Further, the findings can be used as baseline information for further scientific investigation for using papaya leaf as an alternative medicine in curing anemia disease and analyzing phytochemical, pharmaceutical and other biological activities on other plant or vegetable and to conducted further research by using female mice and pregnant female mice.
Ethnomycology of Bracket Fungi in Baduy Tribe Indonesia Khastini, Rida Oktorida; Wahyuni, Indria; Saraswati, Irma
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14082

Abstract

Inhabitants of the Kendeng mountain region depend on the biodiversity of the forest resources for their livelihood. Mushrooms are important resource that provide benefits to mankind. The aim on this research was to document traditional knowledge in bracket fungi utilization to treat health problems in Baduy tribe community. The results described in this paper were obtained through the exploration and identification of bracket fungi as well as the interview to determine their cultural significance for Baduy people. Quantitative approaches were used to determine the Use Values (UV), Informant Consensus Factor (ICF) and Fidelity level (FL) values. A total of 6 species that were identified as bracket fungi were included to Basidiomycota. The Baduy community considers the main use of bracket fungi as a medicine for several ailments. The highest UV, ICF and FL value were obtained by Ganoderma lucidum. This study provides the information regarding bracket fungi species with the high use values that could be employed in pharmacological research and further biotechnological approaches in order to achieve an adequate revenue.
Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis Wahyuningsih, Sri Puji Astuti; Pramudya, Manikya; Sugiharto, Sugiharto
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.4969

Abstract

Mycobacterium tuberculosis is a major infection agent of tuberculosis that is controlled by the response of cell-mediated immunity. It is macrophages and cytolytic T lymphocytes. Activated macrophages will produce free radicals. Excessive free radicals cause tissue damage. Polysaccharide krestin contains ?-glucan. It is a scavenger of free radicals. This research aimed to identify the influence of polysaccharide krestin from C. versicolor on nitrite and malondialdehyde concentrations of mice serum exposed by M. tuberculosis. Nitrite concentration was determined by nitrite assay. Malondialdehyde concentration was determined by TBARS assay. The result showed that adding polysaccharide krestin before exposure (P1) and adding polysaccharide krestin before-after exposure (P3) had the best potential to decrease nitrite concentration. Nitrite concentrations of P1 and P3 were 1.364 0.523 M and 1.456 0.712 M respectively. Meanwhile, P1 group and adding polysaccharide krestin after exposure (P2) had the best potential to decrease malondialdehyde concentration. Malondialdehyde concentrations of P1 and P2 were 1125.86 97.96 M and 953.86 328.16 M respectively. Their nitrite and malondialdehyde concentrations decreased, compared to K and K- groups. The research conclusion was that adding polysaccharide krestin before exposure could decrease both nitrite and malondialdehyde concentrations.How to CiteWahyuningsih, S., Pramudya, M., & Sugiharto, S. (2016). Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis. Biosaintifika: Journal of Biology & Biology Education, 8(1), 12-17.
KONSERVASI BERUANG MADU DI KWPLH BALIKPAPAN Ngabekti, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 2 (2013): September 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i2.2751

Abstract

AbstrakBeruang madu (Helarctos malayanus) merupakan salah satu fauna yang dilindungi perundang-undangan Indonesia. Upaya yang dapat dilakukan untuk mencegah kepunahan beruang madu adalah melalui konservasi. Kawasan Wisata Pendidikan Lingkungan Hidup (KWPLH) beruang madu di Balikpapan dinilai memiliki cara konservasi ex-situ yang terbaik di Asia. Penelitian ini bertujuan mengkaji cara konservasi beruang madu di KWPLH Balikpapan. Metode penelitian yang digunakan adalah metode survei langsung, wawancara, dan analisis dokumen. Hasil penelitian menunjukkan bahwa secara fisik, ekologis, dan aspek sosial masyarakat, KWPLH beruang madu Balikpapan cukup baik sebagai habitat untuk konservasi beruang madu, sekaligus sebagai kawasan untuk pembelajaran lingkungan hidup. Cara konservasi ini layak digunakan sebagai model konservasi satwa liar yang lain. Saran yang dapat disampaikan adalah perluasan area enklosur beruang madu sesuai dengan daerah jelajahnya, sehingga diharapkan dapat bereproduksi secara normal. Dengan demikian keberhasilan konservasi secara ex-situ dapat dicapai.AbstractSun Bear (Helarctos malayanus) is one of the protected fauna legislation Indonesia. Efforts should be made to prevent the extinction of the sun bear is through conservation. Sun Bear Kawasan Wisata Pendidikan Lingkungan Hidup (KWPLH) in Balikpapan is considered have the best way of ex-situ conservation in Asia. This study aims to study how the sun bear conservation in KWPLH Balikpapan. The research method used are direct surveys, interviews, and document analysis. The results showed that physically, ecologically, and as society aspect, Sun Bear KWPLH Balikpapan is good enough as a habitat for the conservation of the sun bear, as well as the area for environmental learning. This conservation is feasible used as a wildlife conservation model. We suggest that the xpansion area of sun bears enclosure is feasible to its home range, so it is expected to have normal reproduction. Thus the success of ex -situ conservation can be achieved.

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