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Klinikal Sains (Jurnal Analis Kesehatan)
Published by Universitas Abdurrab
ISSN : 23384921     EISSN : 26141515     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology
Jurnal klinikal sains program studi analis kesehatan adalah jurnal ilmiah yang membahas tentang kompetensi analis kesehatan yang meliputi kompetensi Mikrobiologi, Hematologi, Parasitologi, Imunoserologi, Toxikologi, dan Kimia Klinik. Jurnal klinikal sains akan terbit setiap enam bulan, dalam satu periode setiap tahun ajaran akan terbit dua kali yakni pada bulan Juni dan Desember.
Arjuna Subject : -
Articles 245 Documents
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MODIFIKASI AMONIUM OKSALAT DENGAN PEWARNA ALAMI BIT MERAH SEBAGAI FORMULA BARU REAGEN HITUNG JUMLAH TROMBOSIT Kartika - Ikawati; Agus Widodo
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5197

Abstract

Abstract Platelet count using the Brecker koncrete method with 1% Ammonium oxalate reagent has a weakness that it cannot color platelets so that they are difficult to count. Red beet contains natural dyes that can color platelet organelles. This study aims to prove the ability of Ammonium oxalate modification with natural red beet dye (Beta vulgaris L) as a new formula for platelet count reagents. Method. This type of research is a laboratory experiment with a comparison of platelet counts using 1% Ammonium oxalate reagent and modification of Ammonium oxalate-red beet extract in a ratio of 1: 1, 1: 2, 2: 1 and automatic methods. The object of research is EDTA venous blood from 30 samples of AAK 17 Semarang students taken using the convenience sampling technique. Manual platelet counts using the Brecker concrete method with a counting chamber and an automatic method using a hematology analyzer. The sharpness of platelet staining is assessed under a microscope. The comparison test uses Repeated Anova. The results of the study showed that the color of platelets examined with the Ammonium-bit modification reagent (1: 2) was sharper with an average number approaching the gold standard. The statistical test results obtained sig 0.069 (> 0.05) or no significant difference. Beet extract (Beta vulgaris L) has the potential as an alternative natural dye in manual platelet counts (Brecer koncrite) keywords : trombosit, red bit, Amonium oksalat
AKTIVITAS ANTIBAKTERI EKSTRAK KULIT MANGGIS (Garcinia mangostana L.) TERHADAP BAKTERI Haemophilus influenzae Firdha Rachmawati; Novie Elvinawaty Mauliku; Arina Novilla; Rosa Nur'ainun Fauziyyah
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5212

Abstract

Respiratory tract infections caused by the bacterium Haemophilus influenzae is a significant health concern due to the bacteria's resistance. Mangosteen peel extract (Garcinia mangostana L.) has potential as an antibacterial agent but is rarely applied to H. influenzae. This study aims to evaluate the antibacterial ability of mangosteen peel extract against the growth of H. influenzae. The mangosteen peel was extracted using the maceration method with ethyl acetate, and n-hexane as solvents. Through the diffusion method, ethyl acetate and n-hexane extracts at concentrations of 15; 30; 62,5; and 125 mg/mL were able to inhibit the growth of H. influenzae. The largest inhibition zone was observed at a concentration of 125 mg/mL, with an average diameter of 7,25 ± 0,07 mm for the n-hexane extract and 8,7 ± 0,71 mm for the ethyl acetate extract. It can be concluded that n-hexane and ethyl acetate extracts have antibacterial activity against the respiratory infection-causing bacterium H. influenzae, with the highest concentration being 125 mg/mL.
UJI AKTIVITAS ANALGESIK EKSTRAK KULIT PISANG KEPOK (Musa paradisiaca L.) PADA MENCIT JANTAN (Mus musculus) Oktavia Nia Agustin; Asri Wido Mukti; Dewi Perwito Sari
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5256

Abstract

Unpleasant multidimensional sensory experience When tissue is damaged is the meaning of pain. However, the pain can be managed by taking painkillers, but the use of painkillers causes several side effects, one of which is stomach irritation. Banana plants are an alternative that is widely used to produce medicine from natural ingredients. Kepok banana peels are waste that has not been utilized optimally. With 5 groups each consisting of 5 mice, this study used the hot plate method to evaluate the analgesic activity of kepok banana peel extract (Musa paradisiaca L.) in male mice (Mus musculus). These groups included the negative control group (CMC Na 0.5%), positive control group (Diclofenac sodium 0.13 mg/20 gBW), and kepok banana peel extract group (1000 mg/kgBW, 2000 mg/kgBW, and 3000 mg/kgBW). The results of the research showed that kepok banana peel extract (Musa paradisiaca L.) had analgesic activity on male mice (Mus musculus) utilizing the Rapid Heating technique, the % MPE value for Kepok Banana Peel Extract (1000 mg/kg BW) of 19.33%, Banana Peel Extract Kepok (2000 mg/kg BB) of 40.43%, and Kepok Banana Peel Extract (3000 mg/kg BB) of 43.88%. Varying doses of kepok banana peel extract (Musa paradisiaca L.) had an influence on analgesic activity in male mice (Mus musculus) with a p value of 0.001 (<0.05). Keywords: Analgesic, Hot plate, Mus musculus, Musa paradisiaca L.
KACANG KEDELAI HITAM DAN KACANG HIJAU SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN Pseudomonas aeruginosa ATCC 15442 TERHADAP MEDIA PAB Vivi Zumrotin; Dian Rachma Wijayanti; Intan Kurniawati Pramitaningrum
Klinikal Sains : Jurnal Analis Kesehatan Vol. 13 No. 1 (2025): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikalsains.v13i1.5280

Abstract

Black soybeans (Glycine soja L. Merr) and green beans (Vigna radiata) are sources of protein and carbohydrates. The background of this study is the lack of development of natural semi-synthetic microbiological media from beans. The purpose of this study was to determine the growth and optimum concentration of Pseudomonas aeruginosa ATCC 15442 bacteria on alternative black soybean media, alternative green bean media with PAB control media. This type of research is a continuous experiment, posttest only control group design. The samples in this study were alternative black soybean and green bean media with concentrations of 5.5%, 10.5%, and 15.5%. The research data were analyzed descriptively and statistically. The results of the study showed that P. aeruginosa ATCC 15442 bacteria grew in all treatments. The average number of P. aeruginosa bacteria on PAB media was 64.67 x  CFU/mL. The average number of P. aeruginosa bacteria in the alternative black soybean media with a concentration of 15.5% was 77 x CFU/mL. The average number of P. aeruginosa bacteria in the alternative mung bean media with a concentration of 15.5% was 91.33 x  CFU/mL. The results of the statistical analysis of the One Way ANOVA Test followed by the Post Hoc Test obtained a significance value of 1.000 and 0.999. The alternative black soybean media and the alternative mung bean media with a concentration of 15.5% had a higher average number of colonies than the PAB control media.
GAMBARAN NILAI HEMATOKRIT PADA PASIEN TUBERCOLOSIS DENGAN MENGGUNAKAN DARAH VENA DI RUMAH SAKIT UMUM DAERAH LABUANG BAJI MAKASSAR Rahmat Saleh; Tary Uswatun Hasanah; Dita Ellyana Artha; Aisyah Aisyah; Suharsih Thahir
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5281

Abstract

Tuberculosis is one of the diseases that can be transmitted through the air caused by the bacteria Mycobacterium tubercolosis. The purpose of this study was to determine the hematocrit picture in tuberculosis patients. The method used in this study is descriptive quantitative. The research sample was examined directly using the macrohematocrit method. The procedure in this study includes several stages, namely the pre-analytical, analytical, and post-analytical stages. In the pre-analytical stage, the researcher prepared the tools and materials to be used. Analytical stage, at this stage the researcher took samples and prepared samples, Post-analytical stage, at this stage the researcher calculated the normal hematocrit value. Based on the results of the study, it showed that there were 4 people with low hematocrit values ​​and 4 people with high hematocrit values. While the hematocrit value in men had a low hematocrit value of 2 people and a high hematocrit value of 2 people, while in women the hematocrit value was low as many as 2 people and a high hematocrit value of 2 people. In this study, there was no difference in hematocrit values ​​between men and women.
UJI ANTIMIKROBA EKSTRAK DAUN CIPLUKAN (Physalis angulata L.) TERHADAP PERTUMBUHAN BAKTERI Streptococcus pyogenes Anas Fadli Wijaya; Rian Anggia Destiawan; Ayu Tri Agustin; Hartalina Mufidah; Mutia Hariani Nurjanah
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5282

Abstract

Infectious diseases caused by microbes in Indonesia are mostly due to bacterial infections. One of the bacteria responsible is Streptococcus pyogenes, which causes Acute Respiratory Infections. Treatment of infections caused by S. pyogenes mostly involves the use of antibiotics. In some cases, antibiotics often lead to resistance, necessitating alternative solutions, such as antimicrobial compounds derived from natural sources like flavonoids, one of which can be found in the extract of ground cherry leaf (Physalis angulata L.). The aim of this study is to investigate the antibacterial effects of a 70% ethanol extract of ground cherry leaves on the growth of S. pyogenes. This research is an experimental laboratory study using a post-test control group design only. The determination of antimicrobial activity was carried out using the well diffusion method. The ground cherry leaf extract, diluted with 10% DMSO, was pipetted into the wells. The positive control used chloramphenicol, while the negative control used 10% DMSO. Based on the observations, an inhibition zone was formed in the positive control with an average of 26.18 ± 1.88 mm, treatment 1 with 100% ground cherry leaf extract 16.22 ± 1.33 mm, treatment 2 with 50% ciplukan leaf extract 15.09 ± 1.01 mm, and treatment 3 with 25% ground cherry leaf extract 12.85 ± 0.87 mm.
RESISTENSI ANTIBIOTIK DAN PERTUMBUHAN STAPHYLOCOCCUS AUREUS PADA MEDIA AGAR DARAH Aldinova Gondo Ruming Asmoro; Yusuf Eko Nugroho; Akhmad Mubarok
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5283

Abstract

A microorganism growth medium is a material containing nutrients that support the growth and development of microorganisms. This nutrient is called the culture medium, and the growth process is known as culture, which can be enriched by the addition of blood. The aim of the researchers was to examine human blood, sheep's blood and cow's blood as growth media for Staphylococcus aureus bacteria. Is there a difference in the resistance test and growth patterns of Staplococcous aureus bacteria in human blood, sheep's blood and cow's blood media. Growth media in human blood, sheep's blood and cow's blood media. Bacterial growth in human blood media with round-shaped colonies, yellowish white with convex elevation, smooth surface, and medium size on cattle blood with a different type of hemolysis with negative results. smooth, and medium sized on sheep blood media with different types of hemolysis with positive results. Bacterial growth on cow blood media bacterial growth on cow blood media colonies are round, yellowish white with convex elevation, smooth surface, and medium sized on cow blood with hemolysis type which is different from a negative result. conclusion is that the Stapylococcus aureus bacteria has the same inhibitory zone in human blood, sheep blood and cow blood media. There is no difference between human blood, sheep blood and cow blood media. The difference in the type of hemolysis in sheep blood is because it has beta hemolysis type and in human blood, and cow's blood has gamma hemolysis.. Key words: Staphylococcous aureus, growth patterns, blood agar media
ANALISIS KADAR ASAM LAKTAT DENGAN INTENSITAS LOW BACK PAIN PADA PEMANEN SAWIT PT X KABUPATEN KAMPAR yuharika pratiwi; Hengky Ardian; Nova Melinda
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5346

Abstract

Background: Repetitive movements and poor body posture during work often cause low back pain (LBP), which radiates pain from the corner of the lowest ribs to the corner of the buttocks in the lumbar-sacral area. The prevalence of LBP ranges between 60 - 80% in the world. Risk factors for LBP in workers are excessive workload on the bones, which causes injury or trauma to soft tissue. Palm harvesters often experience muscle tension due to awkward postures for long periods, resulting in increased lactic acid levels, which are related to the intensity of LBP. An initial survey of PT Objective: To determine the analysis of the relationship between lactic acid levels and low back pain in palm oil harvesters at PT. X Kampar Regency Method: This research is an observational analytic, cross-sectional design. The sampling technique uses total sampling, with statistical tests using the Spearman test. Results: The results of the Spearman correlation test showed that p-value = 0.000; r 0.649 with a strong positive relationship strength. Conclusion: There is a relationship between lactic acid levels and LBP intensity in PT palm oil harvesters. X.
INDEKS ERITROSIT PADA PRE EKLAMPSI Nathalya Dwi Kartika Sari; Hartatiek Nila; Ersalina Nidianti; Widya Novita Sari
Klinikal Sains : Jurnal Analis Kesehatan Vol. 13 No. 1 (2025): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikalsains.v13i1.5390

Abstract

Hypertension in pregnancy ranks as the top cause of death in Indonesia with a value of 33%. Systemic inflammation can be measured using various biochemical and hematological parameters. Preeclampsia (PE) is a complication due to pregnancy that has dangerous risks. Preeclampsia is characterized by high blood pressure and proteinuria. This condition generally appears when the gestational age reaches 20 weeks, is associated with placental hypoperfusion which is the main cause of fetal growth restriction and in severe cases develops into multi-organ dysfunction and even death of the mother and fetus. The aim of this research is to determine the Erythrocyte Index in pregnancies with preeclampsia. The research method used a cross sectional method obtained from medical record data for 1 year (August 2022 to August 2023) from complete blood tests (erythrocyte index) of normal pregnancies and preeclampsia pregnancies carried out at RSI Surabaya A Yani. This research was conducted on pregnant women with preeclampsia and healthy pregnant women at the RSI Surabaya A Yani obstetrics clinic
PROFIL HEMATOLOGI PADA PENDERITA TUBERKULOSIS SEBELUM DAN SESUDAH PENGOBATAN OAT DI PUSKESMAS KOTA DENPASAR I Gusti Agung Ayu Satwikha Dewi; Ida Ayu Manik Partha Sutema; Ni Putu Widiantari; Ni Putu Diah Monica Yanti; Ni Ketut Sari Merta Dewi
Klinikal Sains : Jurnal Analis Kesehatan Vol. 13 No. 1 (2025): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikalsains.v13i1.5447

Abstract

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis that attacks the lungs as well as several systems such as gastrointestinal, central nervous, and liver. TB cases have become a global issue, in 2022 Bali has 3,040 cases and Denpasar City has the highest number reaching 1,063 cases. TB treatment is crucial to be handled effectively and efficiently. Examination of hematologic profiles provides important information about the impact of TB and response to treatment. Currently, there is little information on the relationship between hematology profile and OAT treatment of TB patients at the primary health care level such as puskesmas. The purpose of this study was to examine the hematological profile of TB patients before and after OAT treatment at the Denpasar City Health Center. The sampling technique in this study was accidental sampling conducted at the Denpasar City Health Center in January-September 2024. This study was a prospective cohort study supported by literature review. Hematology profile examination of 32 EDTA blood samples was examined using a hematology analyzer including hemoglobin, erythrocyte, leukocyte, platelet, and ESR profiles. Paired T-test results showed that the four hematological profiles had a sig. value smaller than 0.05 (p<0.05), indicating that there was a significant difference between the hematological profiles of TB patients before and after OAT treatment at the Denpasar City Health Center.

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