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Klinikal Sains (Jurnal Analis Kesehatan)
Published by Universitas Abdurrab
ISSN : 23384921     EISSN : 26141515     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology
Jurnal klinikal sains program studi analis kesehatan adalah jurnal ilmiah yang membahas tentang kompetensi analis kesehatan yang meliputi kompetensi Mikrobiologi, Hematologi, Parasitologi, Imunoserologi, Toxikologi, dan Kimia Klinik. Jurnal klinikal sains akan terbit setiap enam bulan, dalam satu periode setiap tahun ajaran akan terbit dua kali yakni pada bulan Juni dan Desember.
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Articles 245 Documents
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ANALISIS PERBANDINGAN KADAR FLAVONOID TOTAL TEH HIJAU DAN TEH HITAM DENGAN MENGGUNAKAN METODE SPEKTROFOTOMETRI UV-VIS Antonia Vani Kurniawati; .A.K Pramushinta; A.S. Sinulingga; Novamei Indriani
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.4986

Abstract

Tea leaves (Camellia sinensis) are a functional plant that is useful for preventing cancer, maintaining immunity and preventing premature aging. Flavonoids are one of the largest groups of polyphenols which are active compounds from tea leaves (Camellia sinensis). This study aims to determine the total flavonoid content of green tea leaf extract and black tea leaf extract using 2 extraction methods, namely maceration with 96% ethanol solvent and infusion using water solvent. The method in this research uses qualitative tests (Wilstarter cyanidin and NaOH 10%) and quantitative tests (UV-Vis Spectrophometry) and conclusions will be drawn using the SPSS statistical test T (independent)-test. The results of the qualitative test using the starter cyanidin and 10% NaOH were positive for containing flavonoids due to a reddish color change. In the quantitative test, the assay was carried out using UV-Vis spectrophotometry with a wavelength of 434 nm. In this study, the total flavonoid content obtained in the green tea maceration method was 15.728% w/w ± 0.209, black tea maceration 13.357% w/w ± 0.737, green tea infusion 4.153% w/w ± 0.124 and black tea infusion 3.320% w /b ± 0.352. There is a significant difference between green tea and black tea using both the infusion and maceration methods as shown by a sig value ≤ 0.05.
PENGARUH JENIS PELARUT EKSTRAKSI TERHADAP KADAR ALKALOID DALAM EKSTRAK TERIPANG (Paracaudina australis) MENGGUNAKAN SPEKTROFOTOMETRI UV-VIS Aulya Sagita Rahma Sujana; Prisma Trida Hardani; Amanda Safithri Sinulingga
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.4993

Abstract

Sea cucumbers are a type of marine organism that move slowly and live in shallow waters such as sand, mud, algae, coral reefs where sea cucumbers are a source of animal protein consumed by the public. Research on various types of sea cucumbers has shown that there are several bioactive compounds, one of which is alkaloids. Alkaloids are one of the secondary metabolite compounds that are often found in nature, such as in plant and animal tissues. Previous research results show that there are alkaloids in sea cucumber extract. The aim of this research is to determine how this limited type of solvent impacts the amount of bioactive compounds contained in sea cucumbers (Paracaudina australis). Qualitative and quantitative tests were carried out to test alkaloid compounds. Qualitative tests use color reactions with Dragendroff, Wagner and Mayer reagents. Quantitative tests were carried out using Uv-Vis spectrophotometry with a maximum wavelength of 273 nm. The standard solution used is caffeine. Quantitative analysis showed that all extracts with various solvents were positive for containing alkaloids. The highest alkaloid content is ethyl acetate extract with levels of 71.0116 ± 0.2872 ppm, then followed by acetone extract which is semipolar with levels of 22.6531 ± 0.2277 ppm and the lowest is chloroform extract with levels of 13.9622 ± 0.0283 ppm. Keywords: Sea cucumber; Alkaloids; Solvent Type; Uv-Vis Spectophotometry
ANALISA PEMANTAPAN MUTU INTERNAL TAHAP PRA-ANALITIK PEMERIKSAAN HEMATOLOGI Bastian Bastian; Aziyo Kurniawan; Aristoteles Aristoteles
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5013

Abstract

Stages pre-analytic are stages beginning in an inspection laboratory. Errors on stage pre-analytic account for 70% of frequent errors in the laboratory. To anticipate errors, the so-done effort stabilizes quality. To increase quality service and repair quality results. The Objectives: Is to stabilize internal quality during the pre-analytic stage of hematology in the laboratory at House Sick Type C, Palembang. Study this method is descriptive-quantitatvie with a purposive sampling technique in the officer laboratories “X” and “Y”, respectively total 15 respondents. Results: In the “X” hospital laboratory has an average result of 96% and is said to be good, while in the “Y” hospital laboratory, it has an average 97% and is said to be good. That there is no significant difference between the second House Sick. Conclusion: Stabilization of internal quality stage pre-analytical inspection hematology on officers in the laboratories of Hospital “X” and Hospital “Y” Palembang can be stated as good.
UJI TOKSISITAS SUBAKUT CAMPURAN EKSTRAK BIDARA ARAB DAN SAGA POHON TERHADAP KADAR SERUM GLUTAMIC OXALOACERTIC TRANSMINASE DAN SERUM GLUTAMIC PYRUVIC TRANSMINASE PADA TIKUS Nur A'isyah Oktavianingrum; Ersalina Nidianti; Alifia Qurotaaini; Khoirul Ngibad; Ary Andini; Devyana Dyah Wulandari
Klinikal Sains : Jurnal Analis Kesehatan Vol. 13 No. 1 (2025): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tanaman daun bidara arab dan saga pohon sering dimanfaatkan dalam pengobatantradisional karena sifat antioksidannya (Flavonoid). Menggabungkan ekstrak keduadaun ini bertujuan untuk memaksimalkan efek terapeutik dan mengurangi efeksamping. Namun, dampaknya terhadap Kesehatan hati masih perlu diteliti lebihlanjut, sehingga uji toksisitas penting dilakukan. Penelitian ini bertujuan mengujipotensi toksisitas campuran ekstrak daun bidara arab dan saga pohon terhadap kadarSGOT dan SGPT pada tikus putih (Rattus norvegicus). Metode dari penelitian inimenggunakan eksperimental dengan melibatkan 30 tikus yang dibagi menjadi 5kelompok, masing-masing kelompok terdiri dari 6 ekor tikus (3 jantan & 3 betina):Kelompok kontrol negative, kontrol pembawa (Na CMC 1%), dan tiga kelompokperlakuan (dosis 4000, 8000, dan 10.000 mg/kg BB). Pemberian ekstrak dilakukansecara oral selama 21 hari, dengan pengamatan setiap 24 jam. Hasil uji statistikKruskal-Wallis menunjukkan tidak ada efek toksik pada kadar SGOT dengan nilaisignifikansi 0,299 dan SGPT sebesar 0,149, yang mengindikasikan tidak ada efektoksik pada kadar SGOT dan SGPT. Sehingga, dapat disimpulkan bahwa pemberiancampuran ekstrak daun bidara arab dan saga pohon dalam dosis 4000, 8000 dan10.000 mg/kgBB tidak menunjukkan efek toksik terhadap kadar SGOT dan SGPTpada tikus putih dan aman untuk Kesehatan hati.
UJI TOKSISITAS AKUT LD50 CAMPURAN EKSTRAK DAUN BIDARA ARAB (Ziziphus spina-christi L.) dan DAUN SAGA POHON (Adenanthera pavonina) Dinda Enka Nita; Ersalina Nidianti; Khoirul Ngibad; Suprapto Maat; Bismi Aisyah Rahma Putri
Klinikal Sains : Jurnal Analis Kesehatan Vol. 13 No. 1 (2025): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikalsains.v13i1.5046

Abstract

The mixture of Arabian bidara leaf extract (Ziziphus spina-christi L.) and saga tree leaves (Adenanthera pavonina) is a plant used by the community which is empirically used as a traditional medicine such as hyperlipidemia and hypercholesterolemia. The purpose of mixing these two extracts is to increase the efficacy of both extracts which are rich in antioxidants. However, a study on the acute toxicity test of the use of the mixture of these plants has not been carried out, so an acute toxicity test is needed on both extracts to see the safety of use. The purpose of this study was to determine the symptoms of toxicity and the LD50 value caused after the fusion of 56 male and female Wistar strain white rats (28 males, 28 females) which were then divided into 7 groups. The negative control group was not treated, the carrier control group was given a suspension of 1% Na CMC at a dose of 0 mg/Kg given distilled water and the treatment group was given a mixture of Arabic bidara leaf extract and saga tree leaves 1:1 at a dose of 2000 mg/KgBB, 4000 mg/KgBB, 6000 mg/KgBB, 8000 mg/KgBB, oral sonde was performed once a day then observed after 24 hours for 14 days by looking at the number of deaths and symptoms of toxicity. The results obtained were that the mice experienced symptoms of toxicity, weakness, palpitations and decreased activity, with LD50 in male mice of 4224.511 mg/KgBB, in female mice 3030.354 mg/KgBB categorized as slightly toxic.
PROFIL LIPID PADA LANJUT USIA SEBAGAI FAKTOR RESIKO PENYAKIT METABOLIK DI KENDARI SULAWESI TENGGARA Ani Umar; Firdayanti Firdayanti; Angriani Fusvita
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5089

Abstract

Metabolic diseases are a global health problem that is increasing especially in developed countries (Silva et al., 2020). The incidence is higher in the elderly population due to physiological and metabolic changes associated with aging (Silva et al., 2020). Lipid profile is an important indicator in assessing the risk of metabolic diseases. Blood lipids include total cholesterol, LDL (Low Density Lipoprotein), HDL (high Density Lipoprotein), and triglycerides, playing a major role in the development of cardiovascular disease. (Emmanuel et al., 2022) The purpose of this study was to determine the lipid profile in the elderly as one of the metabolic diseases. This study used a cross-sectional design with a target population of 50 elderly individuals aged 60 years and over. data were collected through direct measurement of lipid profiles including total cholesterol, triglycerides, HDL, and LDL using the CHOD-PAP method (Chlorimetric Derivate- Para Amino Phenazone) using a spectrophotometer. The results of the study showed that from 50 elderly patients, the lipid profile levels obtained were total cholesterol levels in the normal category with an average cost of 1.66, where 66% of respondents obtained normal cholesterol levels, while 34% showed high cholesterol levels. in the triglyceride variable, the results obtained were 72% of respondents had normal triglyceride levels, while 28% experienced an increase. HDL levels showed more positive results, with 82% of respondents having good HDL levels, and only 18% having low HDL levels, and, analysis of LDL levels revealed that only 8% of respondents had high LDL levels, while 92% had normal LDL levels in men and women, TG/HDL limits are <150 and> 40 mg/dl, and cholesterol limits <200 and LDL <150.
PERBANDINGAN HASIL PEMERIKSAAN KOLESTEROL TOTAL MENGGUNAKAN SAMPEL SERUM DAN PLASMA EDTA SEGERA DAN DISIMPAN SELAMA 4 JAM Luvia Isma Hanifah; Dini Puspodewi; Ira Pangesti
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5111

Abstract

Cholesterol is a lipid substance (fat component) being one among other known components, fat being a nutrient that is fulfilled by the body. The purpose of the study was to determine the comparative results of total cholesterol examination using serum and plasma EDTA immediately and stored for 4 hours. This study is analytic using cross sectional research design. The results showed in statistical tests using the Paired Sample T-Test test on serum samples immediately examined and stored for 4 hours found (p=0.015) so that the results (p>0.05) concluded there was no difference in serum samples. EDTA plasma samples immediately examined and stored for 4 hours found the results (p<0.001), comparison of total cholesterol levels using serum and EDTA plasma samples immediately examined and stored for 4 hours found (p<0.001) so that the results (p<0.05) are concluded there is a difference between total cholesterol levels in EDTA plasma immediately examined and stored for 4 hours and serum and EDTA plasma immediately examined and stored for 4 hours. Key Words : Cholesterol, Serum, EDTA Plasma, 4 Hour Delay
UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETIL ASETAT DAUN TIGARON (Crataeva nurvala Buch. Ham) TERHADAP Staphylococcus aureus DENGAN METODE SUMURAN Putri Kartika Sari; Nanda Amelia Rahmi; Fitriyanti Fitriyanti
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5121

Abstract

Staphylococcus aureus infection is characterized by tissue damage accompanied by purulent abscesses. Antibiotics are a group of drugs using to treat and prevent bacterial infections. However, long-term use causes antibiotic resistance. Efforts made to increase antibiotic resistance include using natural ingredients as alternative treatments. Tigaron is an empirical medicinal plant whose leaves are used to treat abscesses, wounds and skin diseases. This research aims to determine the types of secondary metabolite compounds and antibacterial activity against Staphylococcus aureus from ethyl acetate extract of tigaron leaves (Crataeva nurvala Buch. Ham). This research was experimental with a post test only control group design with 7 concentration variations. Extraction made by maceration using ethyl acetate, identification of secondary metabolite using phytochemical screening, antibacterial activity tested using well diffusion method. The results showed that concentration ​​of 0.31 mg/mL, 1.55 mg/mL and 7.75 mg/mL were in medium category. Concentrations of 38.75 mg/mL and 193.75 mg/mL were in strong category. Chloramphenicol as a positive control had strongest inhibition zone diameter. The conclusion are ethyl acetate extract of tigaron leaves contain alkaloid, flavonoid and steroid which inhibiting the growth of Staphylococcus aureus, so it has the potential to be a natural antibiotic using to treat infectious diseases
PENGARUH PENUNDAAN PEMERIKSAAN SPESIMEN URINE DENGAN PENYIMPANAN PADA COOLBOX TERHADAP VIABILITAS BAKTERI Ni Wayan Desi Bintari; Nyoman Sudarma
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5160

Abstract

The natural course of the disease in patients with urinary tract infections (UTIs) is often unpredictable, so a proper diagnosis is needed through accurate laboratory tests. Urine specimen management is important for laboratory staff to pay attention to because delays in examination risk increasing false positive or negative results. The purpose of this study was to determine whether there was an effect of delaying the examination of urine specimens stored in a cool box on the viability of bacteria from urine culture examination results. The type of research was experimental, conducted at the Bacteriology Laboratory of STIKES Wira Medika Bali in June-August 2024. The experimental group consisted of delays in urine specimen examination for 0 hours, 2 hours, 4 hours and 6 hours. The calculation of bacterial viability was carried out by examining urine cultures with a calibrated loop technique. The research data were analyzed using the Kruskal Wallis bivariate test. The results showed that there was no significant difference in the viability of bacteria from urine culture at delays of 0 hours, 2 hours, 4 hours and 6 hours (p> 0.05) stored in a cool box. Urine specimens for calculating bacterial viability were still stable in cool box storage up to a delay time of 6 hours. It is suggested that further research is needed to determine whether there is a difference in bacterial viability in specimens stored in a cool box with a delay time of > 6 hours.
VALIDASI METODE ANALISIS PENETAPAN KADAR SENYAWA ALKALOID PADA EKSTRAK TERIPANG (PARACAUDINA AUSTRALIS) DENGAN METODE SPEKTROFOTOMETRI UV-VIS Nuril - Jelali; Prisma Trida Hardani; Dewi Perwito Sari
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5196

Abstract

Teripang ( Paracaudina australis ) diketahui memiliki aktivitas biologis dan farmakologis karena mengandung banyak senyawa bioaktif, salah satunya adalah alkaloid. Untuk mengetahui kadar alkaloid yang terkandung dalam teripang, maka perlu dilakukan penentuan kadar. Pada penelitian ini, penentuan konsentrasi dilakukan dengan menggunakan analisis instrumental menggunakan metode spektrofotometri UV-Vis. Spektrofotometri UV-Vis merupakan metode analisis yang memanfaatkan panjang gelombang UV dan Visible sebagai daerah serapan untuk mendeteksi senyawa. Tujuan dari penelitian ini adalah untuk melakukan validasi metode analisis penentuan kadar senyawa alkaloid dalam ekstrak teripang menggunakan spektrofotometri UV-Vis. Parameter validasi metode dalam penelitian ini meliputi linearitas, LOD dan LOQ, selektivitas, presisi dan akurasi. Hasil linearitas diperoleh persamaan regresi y = 0,0377x + 0,0635 dengan nilai koefisien korelasi r2 = 0,9992. Nilai LOD yang diperoleh sebesar 0,26 ppm dan nilai LOQ sebesar 0,86 ppm. Selanjutnya terdapat parameter selektivitas yang menunjukkan hasil serapan yang sama antara larutan standar dengan larutan sampel pada panjang gelombang 205 nm dan 273 nm. Hasil pengujian presisi yang dilakukan telah memenuhi persyaratan dengan hasil %RSD yang diperoleh sebesar 2,1%. Parameter akurasi yang diperoleh sebesar 101,68% ± 0,011, 100,66% ± 0,008 dan 101,14% ± 0,003. Hasil identifikasi pada pengujian ini menunjukkan bahwa kandungan alkaloid pada teripang Paracaudina australis sebesar 22,6 ppm ± 0,16. Kesimpulan dari penelitian ini adalah diperoleh metode pengujian yang valid dengan rata-rata kandungan alkaloid sebesar 22,6 ppm ± 0,16. Kata kunci : Alkaloid, Paracaudina australis , spektrofotometri UV-Vis, metode validasi

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