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Ari Khusuma
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Kota mataram,
Nusa tenggara barat
INDONESIA
Jurnal Analis Medika Biosains (JAMBS)
ISSN : 23564075     EISSN : 26562456     DOI : -
Core Subject : Health, Science,
JAMBS (Jurnal Analis Medika Bio Sains) is a journal that provides a forum for publishing articles related to food analysis, mikrobiology, hematolgy, clinical chemistry, parasitology, immunoserology, histology. Scientific articles dealing with the following topics in food analysis, mikrobiology, hematolgy, clinical chemistry, parasitology, immunoserology, histology.
Arjuna Subject : -
Articles 313 Documents
The Difference In The Results Of Urine Protein Levels In The Semi-Quantitative Method Of Esbach In Urine Accommodates 12 Hours With 24 Hours In Patients With Urinary Tract Infections Septiawan, Dandi; Pauzi, Iswari; Tantontos, Erlin Yustin; Wiadnya, Ida Bagus Rai
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 2 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i2.378

Abstract

Urinary tract infection is a common infection in the community caused by the growth of microorganisms in the human urinary tract, urinary tract infection is caused by bacteria by identifying the presence of protein in the urine Proteinuria (urine protein) is a protein found in urine that under normal circumstances does not get a high concentration in urine. Protein esbach gold method  standard urine storage using urine storage 24 hours while in urine sampling can use urine storage 12 hours but quantitatively and scientifically there is no known difference in urine protein levels using the semi-quantitative esbach method. Determine the difference in protein content results measured by the  semi-quantitative esbach method  between urine samples collected for 12 hours and those collected for 24 hours in patients with urinary tract infections. This type of research is Observational Analytic with a cross sectional approach. The sample was in the form of urine in patients with urinary tract infections, the number of samples used a total sampling of 24 samples, the data taken were semi-quantitative urine protein levels  of the esbach method. The collected data was then processed using SPSS with Mann Whitney test analysis. The average urine protein content of the 12-hour semi-quantitative method  of esbach was 0.18 g / l, the average urine protein content of the 24-hour semi-quantitative method  of esbach was 0.39 g / l, the difference in protein levels  of the esbach method  in urine collected 12 hours and 24 hours was 0.21 g / l, mathematically there was a difference while statistically there was no difference with the value of (p) = 0.748. 12-hour and 24-hour urine levels showed no difference in protein levels of semi-quantitative esbach method  in patients with urinary tract infections.
Inventarisasi Bacillus Thuringiensis Dengan Metode Cawan Sebar Pada Habitat Hidup Larva Anopheles Sp Pada Tanaman Eceng Gondok (Eichornia Crassipes) Di Kabupaten Lombok Tengah Putriawati, Putriawati; Inayati, Nurul; Agrijanti, Agrijanti
Jurnal Analis Medika Biosains (JAMBS) Vol 5, No 2 (2018): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v5i2.117

Abstract

Beberapa bahan dapat digunakan untuk menekan larva nyamuk, diantaranya B. thuringiensis yang merupakan mikroba enthopatogenik yang dapat menjadi biolarvasida untuk menekan larva Anopheles sp. Penelitian ini bertujuan untuk menginventarisasi B.thuringiensis dari habitat hidup larva Anopheles sp pada tanaman Eceng gondok di Kabupaten Lombok Tengah (Desa Batujai). Metode yang digunakan pada penelitian ini yaitu deskriptif observatif dengan analisa data secara deskriftif untuk menentukan terdapat atau tidaknya B. Thuringiensis  setelah diinokulasi pada media.Pertumbuhan bakteri  setelah dilakukan preparasi sampel pada suhu 80°C selama 10 menit, diinkubasi pada suhu 30°C selama 24 jam. Kemudian di lakukan identifikasi/pemeriksaan sampel secara makroskopik dan mikroskopik di dapatkan hasil B.thuringiensis terdapat pada sampel larva. Hasil dari identifikasi ini memiliki ciri-ciri yang sama dengan B.thuringiensis yaitu bentuk koloni bulat, pinggiran yang rata, warna koloni putih krim,  permukaan koloni datar dan halus serta sifat Gram yaitu basil Gram positif dengan endospora terletak di terminal/subterminal, dengan adanya kristal pada bakteri ini yang menandakan bahwa bakteri B.thuringiensis ini bersifat toksin terhadap larva Anopheles sp. Beedasarkan penelitian yang telah dilakukan inventarisasi Bacillus thuringiensis terdapat pada sampel Larva Anopheles sp pada tanaman Eceng gondok (Eichornia crassipes) di Kabupaten Lombok Tengah. Namun tidak terdapat Bacillus thuringiensis pada sampel tanah. 
Description Of Hemoglobin Levels And Blood Pressure In Security After Night Picket Lines Getas, I Wayan; Oktapia, Selfi Tri
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 1 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i1.396

Abstract

Night shift security can negatively affect the body's condition because activities carried out at night will reduce the amount of rest the body needs. Reduced rest will have an impact on decreased hemoglobin levels and increased blood pressure or decreased blood pressure. Rest at night is very important because the detoxification process of cell regeneration and the formation of red blood cells usually occurs at night. Objective: To find out the picture of hemoglobin levels and blood pressure on security after night picket. Methods: This study is observational with cross sectional method using purposive sampling technique with a total of 44 respondents. Analysis of results can be obtained descriptively by looking at Hb levels and blood pressure. Results: The results of blood pressure checks on security after night pickets with an average of 145/85 and the highest blood pressure is 201/110 mmHg there are 25 people (57%), the lowest blood pressure check results 114/70 mmHg There are 19 people (43%). For the results of the examination of hemoglobin levels in security after night picket hemoglobin levels with an average of 14.1 and the highest hemoglobin is 17.4 g/dL and normal blood pressure there are 32 people (73%), the lowest hemoglobin level examination results 11.4 g/dL there are 12 people (27%). Conclusion: Night picketing can increase blood pressure and reduce hemoglobin levels in security.
Methicillin–resistant Staphylococcus aureus (MRSA) Identification Among Health Care Workers in the Intensive Care of Nusa Tenggara Barat Regional General Hospital Pristianingrum, Santy; Dasi, Palika Gita Dewi; Zainiati, Baiq Lely; Budi, Endar Trisno; Rahmah, Laila Annisa; Damayanti, Ida Ayu
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 1 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i1.441

Abstract

Healthcare workers (HCW’s) are the primary providers of services in healthcare facilities. The high frequency of contact between healthcare workers and patients renders them susceptible to the transmission of MRSA. This research was conducted to detect the presence of the MRSA using Mec A gene and their sensitivity with antibiotics among HCWs in the intensive care of the Nusa Tenggara Barat Regional General Hospital. This is cross-sectional study with purposive random sampling nostril swabs and information about daily routines of changing outfit HCW’s ICU, HCU, PICU rooms between January-March 2024. The examination included a microbiological examination and a PCR of the Mec A gene.Microbiological examination of 45 HCWs nostril swabs revealed the presence of 31 isolates of S.aureus, 25 of isolate were identified as Methicillin-Susceptible Staphylococcus aureus (MSSA), and 6 as MRSA. Four MRSA isolates from the HCW in ICU exhibited the highest resistant to Cefoxitin (75%), while two HCW’s PICU isolates demonstrated 100% resistant to Amoxicillin. The six MRSA isolates were confirmed positive for the Mec A gene by PCR examination using the Mec A primer at 532 bp, and three of them is a HCW’s who’s didn’t change clothes every day. The presence of the MRSA among HCWs in the intensive care unit of Nusa Tenggara Barat Regional General Hospital was 19%. Furthermore, it was found that all of S.aureus were still sensitif to vancomycin.Test ubah metadata
Pengaruh Kadar Glukosa Urine Metode Benedict, Fehling Dan Stick Setelah Ditambahkan Vitamin C Dosis Tinggi/ 1000 Mg Sulfia, Febrian; Fikri, Zainal; Fauzi, Iswari
Jurnal Analis Medika Biosains (JAMBS) Vol 5, No 2 (2018): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v5i2.118

Abstract

Glukosa urine adalah pemeriksaan urine rutin, pemeriksaan dasar yang dapat dipakai untuk melakukan pemeriksaan laboratorium. Secara rutin pemeriksaan glukosa urine ditekankan terhadap kemungkinan adanya glukosa dalam urine atau glukosuria. Glukosa dalam urine dapat deteksi dengan cara yang berbeda-beda. Pada pemeriksaan glukosa urine sebaiknya penderita jangan makan zat reduktor vitamin C. karena zat tersebut dapat memberikan hasil positif palsu dengan cara reduksi. Tujuan penelitian ini Untuk mengetahui perbedaan kadar glukosa urine metode benedict, fehling, dan stick pada urine setelah ditambahkan vitamin C. Metode Penelitian ini  bersifat Observasional Deskriptif. Penelitian ini berlangsung menggunakan Non Random Purposive Sampling. Sampel urine setelah ditambahkan vitamin C dosis tinggi/ 1000 mg kemudian di periksa menggunakan metode benedict, fehling dan stick. Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian ini positif  kadar glukosa pada urine setelah ditambahkan vitamin C dosis tinggi/ 1000 mg dan setiap metodenya terjadi perbedaan positifitas. Kesimpulan pada penelitian ini adalah urine yang ditambahkan vitamin C dosis tinggi/ 1000 mg berpengaruh secara signifkan terhadap perbedaan kadar glukosa urine  pada metode benedict, fehling dan stick.
Efek Diuretik Daun Dan Buah Kacang Panjang (Vigna Sinensis Var. Sesquipedalis) Pada Hewan Coba Tikus Putih (Rattus Norvegicus) Galur Wistar Iswidhani, Iswidhani; Diarti, Maruni Wiwin; Nurhidayati, Nurhidayati; Jiwintarum, Yunan
Jurnal Analis Medika Biosains (JAMBS) Vol 2, No 1 (2015): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v2i1.28

Abstract

Tujuan umum penelitian ini adalah mengetahui efek diuretik sediaan rebusan dan sediaan segar daun dan buah Kacang panjang  (Vigna sinensis var. sesquipedalis)  pada hewan coba tikus putih (Rattus norvegicus) galur wistar. Penelitian ini adalah penelitian true eksperimental di laboratorium dengan rancangan penelitian menggunakan Rancangan Acak Kelompok (RAK). Ada dua kelompok variabel independent yaitu daun kacang panjang dalam bentuk rebusan dan segar dan buah kacang panjang dalam bentuk rebusan dan segar, sedangkan variabel dependentnya ada tiga yaitu onset, durasi dan volume urine yang dikeluarkan hewan coba tikus putih (Rattus norvegicus) galur wistar. Total perlakuan dalam penelitian ini adalah 18 perlakuan. Hasil penelitian menunjukkan volume urine hewan coba tikus putih pada kelompok K1(kontrol negatif) adalah 22.67 ± 2.5, K2 (kontrol positif)  Furosemid 39.3 ± 2.3, P1 38.67 ± 1.1, P2 33.33 ± 3.0, P3 31.00 ± 2.6, P4 25.33 ± 1.1, P5 40.67 ± 1.1, P6 35.00 ± 1.0, P7 30.67 ± 1.1, P8 26.33 ± 2.0, P9 37.33 ± 3.0, P10 31.00 ± 3.4, P11 29.33 ± 0.5, P12 25.33 ± 1.1, P13 39.33 ± 1.1, P14 32.67 ± 3.2, P15 30.00 ± 2.0, dan P16 25.33 ±2.3 dan rerata onset pengeluaran urine pada hewan coba tikus putih dengan durasi selama 5 jam adalah kelompok K1(kontrol negatif) adalah 9.67 kali, K2 (kontrol positif)  Furosemid 16 kali, P1 16 kali, P214.67 kali, P3 13.3 kali, P411 kali, P5 16 kali, P6 14.33 kali, P7 13 kali, P8 11 kali, P9 16 kali, P10 15.67 kali, P11 14 kali, P12 11.67 kali, P13 16 kali, P14 15.33 kali, P15 13.67 kali, dan P16 11.33 kali. Hasil uji stastistik  Two way Anova  atau Univariate analysis of variance tests of Between-Subjects Effect pada tingkat kepercayaan 95% (Pα = 0.05) menunjukkan bahwa nilai p=0.000<pα 0.05 pada setiap kelompok perlakuan. Kesimpulan terdapat efek diuretik sediaan rebusan dan sediaan segar daun dan buah Kacang panjang  (Vigna sinensis var. sesquipedalis) pada hewan coba tikus putih (Rattus norvegicus) galur wistar diterima.
Cross Test of ICT-TB and Genexpert Examination in Suspected Cases of M. tuberculosis Infection at Bima Regency Regional Hospital Wiadnya, Ida Bagus Rai; Sani, Syahrul; Srigede, Lalu
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 1 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i1.426

Abstract

GeneXpert is able to detect specimens that are positive for M. tuberculosis in a shorter time and with high sensitivity and specificity. ICT-TB is used to detect the presence of antibodies produced by M. tuberculosis infection in the body. This response requires time to form. However, actually ICT-TB is an easier method compared to Genexpert. So this research was carried out to compare the results of the two methods. Research Objective: To determine the results of cross-testing of ICT-TB and Genexpert examinations on cases of suspected M. tubeculosis infection at the Bima Regency Hospital. Research Method: This is an analytical observational study with a cross-sectional design. The samples used in this research were 52 samples and the data were analyzed descriptively. Research Results: The distribution of suspected tuberculosis based on gender is 54% male and 46% female. Based on age group, Toddlers 13.5%, Children 11.5%, Teenagers 9.6%, Adults 44.2% and Elderly 21.2%. ICT-TB examination results are negative. Examination with Genexpert on the same patient was negative. Conclusion: From the results of research using ICT-TB and GeneXpert in patients with suspected M. tuberculosis infection, negative results were obtained.
Immune Response Analysis of Children with Pulmonary TB Using Immuno Chromatography Test -Tuberculosis (ICT - TB) Ariami, Pancawati; Astriani, Astriani; Inayati, Nurul; Khusuma, Ari
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 1 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i1.361

Abstract

Risk factors that increase the spread of Mycobacteria tuberculosis to family members are a history of contact with tuberculosis sufferers and overcrowding at home. If the child has family or close contact with a positive TB sufferer, screening is necessary. Close contact means children live in the same house or often meet the patient. TB testing involves taking sputum samples, but in reality officers often have difficulty taking sputum samples, especially children, so they have to carry out an immunochromatography-tuberculosis (ICT-TB) test. ICT-TB is a serological examination that functions as an alternative screening examination for children who have difficulty collecting sputum. To determine the immune response in children suffering from tuberculosis using the immunochromatography tuberculosis test (ICT-TB). This research is an analytical observational study with a cross-sectional design and uses a saturated sampling method where the entire population is sampled. Purposive sampling was then used to select samples during household visits so that a total sample of 44 samples was obtained.In a sample of 44 children suffering from pulmonary tuberculosis, all ICT-TB test results were negative. 55% of children with suspected pulmonary TB were boys and 45% were boys, the number of children with suspected TB in the 1st, 2nd, 3rd, 4th, 5th and 6th months of treatment was 4.2% and 16.7% respectively. %, 21%, 16.7 %, 33.3%, 8.3% and 18.3%. Based on observations of the children's health status, 43% were healthy and 2% were sick, and in terms of physical factors, the suspect's house had good physical environmental factors. All 44 suspected children had negative ICT-TB test results. Further research needs to be carried out using cohort studies, observing the development of children with tuberculosis over a period of ± 1 to 2 years, and studying contacts in families of non-children aged 0 to 14 years.  
Perbedaan Penyebab Infeksi Parasit Usus Manusia Pada Vektor Lalat Rumah (Musca Domestica) Dan Lalat Hijau (Chrysomya Megacephala) Di Pasar Kota Mataram Akhirah, Masratul; Kristinawati, Erna; Sundayani, Lina; Fihirudin, Fihirudin
Jurnal Analis Medika Biosains (JAMBS) Vol 4, No 1 (2017): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v4i1.129

Abstract

Sanitasi dan lingkungan fisik yang buruk merupakan faktor utama timbulnya berbagai jenis penyakit, terutama penyakit yang disebabkan oleh parasit usus seperti kecacingan, diare dan disentri. Penyakit-penyakit tersebut dapat ditularkan melalui lalat sebagai vektor mekanis pembawa penyakit seperti lalat rumah (Musca domestica) dan lalat hijau (Chrysomya megacephala). Penelitian ini bertujuan untuk mengetahui penyebab infeksi jenis parasit usus yang terdapat pada vektor lalat rumah (Musca domestica) dan lalat hijau (Chrysomya megacephala) di Pasar Kota Mataram. Penelitian yang bersifat observasional deskriptif atau exploratory study dengan sampel lalat rumah Musca domestica) dan lalat hijau (Chrysomya megacephala) masing-masing sebanyak 16 ekor lalat yang diambil dari 4 pasar di Kota Mataram, yaitu pasar Mandalika, pasar Cakranegara, pasar Pagesangan dan pasar Kebon Roek. Sehingga jumlah sampel yang diperiksa sebanyak 8 sampel yang terdiri dari 4 sampel lalat rumah (Musca domestica) dan 4 sampel lalat hijau (Chrysomya megacephala) dimana setiap sampel berisi 16 ekor lalat. Hasil penelitian secara mikroskopis diperoleh persentase sampel positif parasit pada lalat rumah (Musca domestica) sebesar 25% dan lalat hijau (Chrysomya megacephala) sebesar 75% dengan ditemukannya kista dari golongan protozoa usus yakni Balantidium coli dan tidak ditemukan dari golongan nematoda usus. Sehingga tidak terdapat perbedaan penyebab infeksi parasit usus manusia yang terdapat pada vektor lalat rumah (Musca domestica) dan lalat hijau (Chrysomya megacephala)
UJI SENSITIVITAS DAN SPESIFITAS UJI PENYARING KANDIDIASIS VAGINA MENGGUNAKAN SEDIMEN DENGAN PENGECATAN GRAM Sundayani, Lina; Agrijanti, Agrijanti
Jurnal Analis Medika Biosains (JAMBS) Vol 2, No 1 (2015): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v2i1.29

Abstract

Pewarnaan Gram adalah metode berharga dalam diagnosis yang akurat cepat gejala vulvovaginal dan bahkan terkadang lebih unggul dari kultur  karena menunjukkan bentuk invasif dari ragi1. Tujuan penelitian untuk mengetahui Sensitivitas dan Spesivitas Uji penyaring Kandidiasis Vagina dengan Menggunakan Sedimen dengan Pengecatan Gram. Penelitian uji sensitivitas dan spesivitas sediaan Gram menggunakan 25 sampel sedimen urin wanita penderita Diabetes Mellitus sebagai Uji Penyaring  kandidiasis vagina Hasil pemeriksaan sedimen urin dibuat sediaan ditambah KOH 20%  negatif sel ragi 16, Positif sel ragi.9Hasil pemeriksaan sedimen urin dibuat sediaan Gram 18 negatif sel ragi dan 7 positif sel ragi.  Hasil kultur sebagai Gold Standar di media Sabaouroud Glukosa Agar dan Corn Meal Agar diperoleh hasil 18 negatif dan 7 Positif sedimen urine yang tumbuh Candida albicans Hasil perhitungan ditemukan Sensitivitas dan Spesifitas pemeriksaan kandidiasis Vagina dengan menggunakan sedimen urin yang ditambah KOH 10% ialah sebagai berikut Sensitivitas 63,63% Spesifisitas 85,71%. Dari hasil Perhitungan maka ditemukan sensitivitas dan spesifitas metode pemeriksaan dengan menggunakan sedimen yang dibuat sediaan gram     Sensitivitas 87,50% Spesifisitas 93,75%. Hasil Sensitivitas dan Spesivitas pemeriksaan dengan menggunakan sedimen yang dibuat sediaan gram lebih tinggi daripada pemeriksaan Kandidiasis Vagina dengan menggunakan sedimen urin yang ditambah KOH 10%Kata Kunci :

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