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INDONESIA
Jurnal Analis Medika Biosains (JAMBS)
ISSN : 23564075     EISSN : 26562456     DOI : -
Core Subject : Health, Science,
JAMBS (Jurnal Analis Medika Bio Sains) is a journal that provides a forum for publishing articles related to food analysis, mikrobiology, hematolgy, clinical chemistry, parasitology, immunoserology, histology. Scientific articles dealing with the following topics in food analysis, mikrobiology, hematolgy, clinical chemistry, parasitology, immunoserology, histology.
Arjuna Subject : -
Articles 313 Documents
Correlation of Blood Creatinine Levels with The Result of Urine Sediment Analysis in Chronic Kidney Failure Patient Zaetun, Siti; Rohmi, Rohmi; Oktaviani, Andi Dwi; Srigede, Lalu
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.339

Abstract

Chronic Kidney Failure is a condition caused by a gradual and chronic decline in kidney function. This situation can affect to creatinine levels. Creatinine is the result of endogenous metabolism that is useful for assessing glomerular function, in the event of renal dysfunction then the filtration ability of creatinine will be reduced and serum creatinine will increase. Urine sediment is produced from the results of urine concentration consisting of calcium salts. This concentrated urine comes from the kidneys with a variety of causes, one of which is chronic kidney failure, flows through the ureter to the bladder and continues to the urethra until the urethral. Objective: To find out the correlation of blood creatinine levels with the results of urine sediment analysis in patients with chronic kidney failure. Method: This research is observational analytical with a cross sectional approach to determine the correlation between independent and dependent variables. The sample is taken purposive sampling by selecting a sample based on certain criteria. The collected data is then analyzed using Spearman statistical tests. Results: Examination of creatinine levels in chronic kidney failure patients obtained 6.67 mg/dl. The average urine sediment found erythrocytes as many 2-3/HPF to many/HPF. Conclusion: There is a correlation of blood creatinine levels with the results of urine sediment analysis in patients with chronic kidney failure.
Profil differential count pada penderita hepatitis B di RSUD Patut Patuh Patju Kabupaten Lombok Barat aini, aini aini; Mentari, Ika Nurfajri; Zahrah, Laelatur
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 1 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i1.253

Abstract

Hepatitis B merupakan penyakit sistemik yang menyerang organ hati yang disebabkan oleh virus Hepatitis B, penyakit ini bersifat menular. Hepatitis masih menjadi masalah kesehatan di dunia terutama di Negara berkembang termasuk Indonesia. Hepatitis B dapat dideteksi dengan pemeriksaan immunokromatografi dan dilanjutkan dengan pemeriksaan differential count. Tujuan penelitian ini adalah untuk mengetahui profil differential count / hitung jenis leukosit pada penderita Hepatitis B. Penelitian ini bersifat Deskriptif Observasional Analitik dengan menggunakan desain penelitian Cross Sectional Analitik. Penelitian dilakukan di RSUD Patut Patuh Patju terhadap 48 sampel pasien hepatitis dengan HBsAg positif. Hasil yang didapat berupa pemeriksaan Differential count untuk jenis leukosit yang normal Basofil 46 orang (95,83%) Eosinofil 12 orang (25%) Neutrofil 19 orang (39,59%) Limposit 9 orang (18,75%) Monosit 35 orang (72,91%). Untuk jenis basofil tidak mengalami penurunan frekuensi 0 (0%), dan yang mengalami penurunan eosinofil 26 orang (54,16 %) neutrofil 1 orang (2,08%) limposit 39 orang (81,25 %) monosit 1 orang (2,08 %) .Jenis leukosit yang mengalami peningkatan basofil 2 orang (4,16%) eosinofil 10 orang (20,83 %) neutrofil 28 (58,33 %) limposit 0 (0%) monosit 12 orang (25 %). 
The Effect of Sambang Darah Leaf Filtrate (Excoecaria Cochinchinensis L) to Reduce The Bleeding Time in Skin Wounds of White Rats (Rattus Norvegicus) Hijriani, Baiq Isti; Zaetun, Siti; Wiadnya, Ida Bagus Rai
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.298

Abstract

Hemostasis is the process of stopping bleeding from a damaged blood vessel. When bleeding occurs, the body will naturally respond with a hemostatic mechanism to stop the bleeding. Indonesian people have long used plants in medicine, one of which is sambang darah (Excoecaria cochinchinensis L) plant. Sambang darah has a chemical compound that is thought to stop bleeding. The purpose of this study was to determine the potential of sambang darah filtrate to reduce the bleeding time in skin wounds of white rats (Rattus norvegicus). This study is a pre-experimental study with a static group comparison approach. The number of experimental units in this study were 10 white rats which were divided into treatment group and control group. Data analysis used the Independent Sample T Test. The results showed that the average of bleeding time in the skin wounds of white rats without drops of sambang darah leaf filtrate was 252 seconds. While the average of bleeding time in the skin wounds of white rats with drops of sambang darah leaf filtrate is 163 seconds. The conclusion of this study is sambang darah (Excoecaria cochinchinensis L) leaf filtrate have a potential to reduce the bleeding time in the skin wounds of white rats (Rattus norvegicus).  
Utilization Of Coconut Dregs As An Alternative Medium For The Growth Of Bacillus Subtilis And Escherchia Coli Nggua, Dewita; Mahtuti, Erni Yohani
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.331

Abstract

Coconut dregs are waste produced after processing coconuts into coconut oil. Coconut pulp has a high nutritional content which can be used for bacterial growth. The nutritional content of coconut dregs is 38.1% carbohydrates, 5.6% crude protein, 16.3% crude fat, 31.6% crude fiber, 2.6% ash content and 5.5% water content. The research used coconut dregs as an alternative medium for the growth of gram-negative and gram-positive bacteria. In the trials, B. subtilis and E. coli bacteria were used. The type of research used was a true experiment with a completely randomized design. The results of the research showed that the highest average number of colonies on the coconut dregs alternative media was in treatment A1, namely the average of B.subtilis colonies was 50.6 x 10² CFU/mL and E.coli 36 .6 x 10² CFU/mL while the control media averaged B.subtilis colonies 75 x 10² CFU/mL and E.coli 86.3 x 10² CFU/mL. The results of statistical tests using anova p<0.05 with a sig value of 0.001 show that there is an influence of differences in the concentration of coconut dregs and agar as an alternative medium for bacterial growth. The post hoc test compares the concentration between treatment A1 and control with a sig p value of >0.05 (0.250), namely There is no significant difference, while the concentration between treatments A2, A3 and A4 with control has a sig p value <0.05, namely 0.000, meaning there is a significant difference. So treatment A1 is the best treatment for the growth of B.subtilis and E.coli bacteria.
Efek Imunostimulator Kubis (Brassica Oleracea Var. Capitata Alba) Terhadap Titer Imunoglobulin G (Ig G) Pada Kelinci Yang Diinduksi Dengan Sel Darah Merah Domba Inayati, Nurul; Fihiruddin, Fihiruddin; Getas, I Wayan
Jurnal Analis Medika Biosains (JAMBS) Vol 7, No 2 (2020): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v7i2.196

Abstract

Kubis (Brassica aloracae var.capitata alba) adalah salah satu sayuran yang berpotensi sebagai imunostimulator. Kubis banyak megandung nutrien yang mempunyai nutrisi tinggi seperti vitamin, mineral, dietary fiber, glukosinolates, polyphenol dan phenolic acid. Disamping itu juga kubis mengandung peptida yang berperan sebagai imunoglobulin production stimulating factor (IPSF) sehingga dapat berfungsi sebagai imunostimulator yang akan dapat meningkatkan sistem imun tubuh dengan cara merangsang peningkatan sistem fagositik dan produksi interleukin 5. Penelitian ini merupakan penelitian ekprimental dengan desain statistic group comparation yang bertujuan mengetahui pengaruh pemberian kubis (Brassica oleracea capitata alba) untuk meningkatkan pembentukan immunoglobulin G (IgG) pada kelinci yang diinduksi dengan sel darah merah domba 2 %. Hewan coba yang digunakan dikelompokkan menjadi 2 kelompok yaitu kelompok perlakuan dan kelompok kontrol. Hewan coba yang akan digunakan adalah kelinci dengan berat 700 – 800 gr sebanyak 20 ekor yang akan dibagi menjadi dua kelompok yaitu kelompok perlakuan dan kontrol. Metode pemeriksaan yang digunakan adalah dengan uji heamaglutinasi dengan mengamati terjadinya aglutinasi antara serum yang diambil dari darah kelinci dengan sel darah merah domba   (SDMD) 2 %. Rata-rata titer Imunoglobulin G (IgG) pada kelompok perlakuan dan kelompok kontrol masing-masing adalah 2.26 µl/ml dan 1.62 µl/ml. Hasil uji statistik dengan paired t test dengan tingkat kepercayaan 0,95 % menunjukkan bahwa terdapat perbedaan yang signifikan ( p < 0,05) antara kelompok perlakuan dan kelompok kontrol yaitu dengan nilai signifikannya adalah 0,043.
Hubungan Titer Widal Dengan Jumlah Limfosit Dan Trombosit Pada Pasien Demam Typhoid Di Puskesmas Gunungsari Lombok Barat Fitriyani, Fitriyani; Pauzi, Iswari; Jiwantoro, Yudha Anggit
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 2 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i2.245

Abstract

Demam typhoid merupakan infeksi bakteri yang disebabkan oleh Salmonella typhi. Infeksi Salmonella typhi bersifat bakterimia yang masuk ke dalam usus halus kemudian menyebar ke sumsum tulang. Diagnosis Samonella typhi menggunakan uji widal dan pemeriksaan darah rutin. Uji Widal dan pemeriksaan darah rutin untuk melihat mekanisme tubuh terhadap limfosit dan trombosit yang diproduksi di sumsum tulang dan berfungsi sebagai pertahanan tubuh terhadap adanya infeksi. Bila bakteri ini sampai ke sumsum tulang maka akan menghambat pembentukan limfosit dan trombosit. Hal ini juga disebabkan adanya endotoksin dari bakteri sehingga pada kasus demam typhoid terjadinya limfositosis dan trombositopenia. Tujuan penelitian: Mengetahui hubungan titer widal dengan jumlah limfosit dan trombosit pada pasien demam typhoid. Metode penelitian: Observasional Analitik. Jumlah sampel yang digunakan sebanyak 24 sampel dengan menggunakan data primer. Data yang dikumpulkan berupa titer widal dengan jumlah limfosit dan trombosit. Kemudian diolah menggunakan uji statistik Korelasi Person. Hasil Penelitian: Rerata limfosit pada antigen O dengan titer 1/160 adalah 24,84%, titer 1/320 adalah 43,95%, rerata trombosit dengan titer 1/160 adalah 312.000 µL dan titer 1/320 adalah 230.750 µL. Sedangkan pada antigen H rerata jumlah limfosit dengan titer 1/160 adalah  35,48% dan titer 1/320 adalah 39,86%. Rerata jumlah trombosit dengan titer 1/160 adalah 196.670 µL dan titer 1/320 adalah 158.000 µL. Hasil uji statistik p>0,05. Kesimpulan: Tidak terdapat hubungan titer widal dengan jumlah limfosit dan trombosit pada pasien demam typhoid di Puskesmas Gunungsari Lombok Barat.
Hubungan Kepadatan Parasit Terhadap Kadar Hemoglobin Pada Penderita Malaria Asimtomatik Di Gunung Sari Agustin, Ermadiana; Resnhaleksmana, Ersandhi; Getas, I Wayan
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 1 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i1.260

Abstract

Malaria is an infectious disease that still provides a high morbidity rate in the world.  Malaria affects almost all blood components, one of which is hemoglobin levels. In malaria infection, there is a decrease in hemoglobin levels due to the destruction of excessive red blood cells by the malaria parasite. Asymptomatic malaria infection plays an important role in malaria transmission as it is a major influence on ongoing treatment-based malaria control programs. The research objective in this study was to know the effects of parasite density on hemoglobin levels in patients with Asymptomatic Malaria disease in Gunung Sari. The research study used is an analytic observational study with a Cross-Sectional approach with the Accidental sampling method and the Consecutive sampling method. The result of the study is out of the 14 samples diagnosed with asymptomatic malaria, 12 people (85.7%) had a mild degree of parasitemia based on parasite density calculations (8-800 / μl), and 2 people (14.3%) had moderate degrees of parasitemia (801- 8800 / μl). As many as 6 people (42.9%) had normal Hb levels, and 8 people (57.1%) had Hb levels below normal values. The conclusion of this study proved that there is a significant correlation between parasite density and hemoglobin levels in asymptomatic malaria patients in Gunung Sari. 
Lemongrass Powder in Bio-Bs Effervescent Formulation of Lombok Island Local Isolate on Viability and Amount of Bacillus Sphaericus Toxin Crystal Protein for Control of Anopheles sp. Larvae Jiwintarum, Yunan; Fikri, Zainal; Diarti, Maruni Wiwin; Sulaimah, Rabi'unnisa
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.266

Abstract

Bio Formula – BS effervescent (Bio- Bacillus sphaericus) is an Effervescent powder-shaped formula that is easy to use by the public. Lombok's local effervescent bio-BS isolated formula has a weakness in terms of smell. The results from the literature search that fragrant lemongrass has a distinctive smell and can kill Aedes aeygpti, but scientific data has not been obtained about the ability of fragrant lemongrass to kill Anopheles Sp in the form of larvae and mosquitoes. The study aimed to find out the effect of the combination formulation of Bio-BS Effervescent local isolate Lombok Island with the addition of lemongrass powder for viability and the amount of toxin crystal protein Bacillus sphaericus. This research method is exploratory and experimental in the laboratory with the design of Post Test Only Control Group Design. The study used six treatment formulations. Samples of Anopheles sp larvae research in Batu Layar lagoon, West Lombok regency and pelur lagoon in Peringgesela, East Lombok regency and from the results of colonization of larvae. The independent Variable is a combination formulation of Bio-BS Effervescent isolated locally from Lombok Island with the addition of fragrant lemongrass powder. The dependent variable is the mortality of Larvae Anophels Sp, Viability of B. Sphaericus, and Amount of Toxin Protein Production of B. Sphaericus. The larvae death rate, the concentration of cells/endospores, and the number of repeats in each container are then tabulated and analyzed using Probit Analysis with the help of MINITAB 16 software. B. sphaericus viability data and the amount of endospore toxin protein crystal production were descriptively analyzed.
Differences In Counting The Number of Leukocytes Using Cermai Fruit (Phyllantus acidus) Juice and Turk Solution Using The Counting Room Method Effendi, Akhmad Rizal; Nugraha, Gilang; Santoso, Andreas Putro Ragil
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 2 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i2.324

Abstract

Leukocytes play a role in the body's defense system. Counting the number of leukocytes can be done manually using turk solution. Glacial acetic acid in turk solution functions to lyse erythrocytes but this compound is included in compounds that are carcinogenic. There is a natural ingredient that can lyse blood cells other than leukocytes, namely Cermai fruit. Cermai fruit contains a weak acid, namely citric acid of 39,8% so that it can lyse blood cells other than leukocytes. This study aims to analyze the difference in the number of leukocytes in cermai fruit juice and turk solution. This type of research is experimental with simple random sampling technique. The sample of this research is cermai fruit which will be used to count the number of leukocytes in 10 respondents. The mean number of leukocytes in the control solution was 6.610 cells/mm3, 10% concentration 4.296 cells/mm3, 20% concentration 4.227 cells/mm3, 30% concentration 5.918 cells/mm3 and 100% concentration 5.745 cells/mm3. Data were analyzed using the Kruskal Wallis test and the sig. 0.001 <0,05 so H1 is accepted, which means there is a significant difference, so it is continued with the Post Hoc test. Post hoc test showed that there was no significant difference in the results of leukocyte counts using cermai fruit with concentrations of 30% and 100% compared to the control (p>0,05). Meaning that only the concentrations of 30% and 100% showed the suitability of the number of leukocytes with the control so that these concentrations could be used as an alternative to 3% glacial acetic acid in turksolution.
Jenis dan Jumlah Sedimen Urine Organik dan Anorganik pada Penderita Demam Typhoid Sebelum dan setelah Pemberian Antibiotik Diarti, Maruni Wiwin; Fikri, Zainal; Sintia Dewi, Ni Kadek; Jiwintarum, Yunan
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 1 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i1.222

Abstract

Salmonella typhi merupakan kuman pathogen penyebab demam typhoid yaitu suatu penyakit infeksi sistemik dengan gejala demam, gangguan saluran cerna, dan mual muntah. Demam dengan suhu yang tinggi, akan terjadi komplikasi seperti perubahan pada urin yang menjadi lebih gelap karena pecahnya sejumlah sel darah merah, dan gagal ginjal. Diagnosis tersebut perlu membutuhkan pemeriksaan, seperti pemeriksaan sedimen urine. Tujuan dari penelitian ini adalah untuk mengetahui perbedaan jenis dan jumlah sedimen urine pada penderita demam typhoid sebelum dan setelahpemberianAntibiotik. Penelitian ini menggunakan rancangan penelitian observasional deskriptif dengan pendekatan cross sectional. Populasi pasien yang positif demam typhoid di Puskesmas Gerung Kabupaten Lombok Barat dan sampel sebanyak 23 orang. Analisis data menggunakan deskriptif.  Hasil penelitian menunjukkan jumlah sedimen urine sebelum pemberian Antibiotik adalah leukosit dan eritrosit abnormal (13,0%), epitel abnormal (47,8%), Kristal klasium abnormal (4,3%). Jumlah sedimen urine setelah pemberian Antibiotik adalah leukosit normal (100%), tidak ditemukan leukosit abnormal, eritrosit normal (56,5%) dan tidak ditemukannya eritrosit abnormal, sel epitel normal (60,8%), dan tidak ditemukannya sel epitel abnormal.

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