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Jurnal Analis Medika Biosains (JAMBS)
Published by Poltekkes Kemenkes Mataram
ISSN : 23564075     EISSN : 26562456     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions
JAMBS (Jurnal Analis Medika Bio Sains) is a journal that provides a forum for publishing articles related to food analysis, mikrobiology, hematolgy, clinical chemistry, parasitology, immunoserology, histology. Scientific articles dealing with the following topics in food analysis, mikrobiology, hematolgy, clinical chemistry, parasitology, immunoserology, histology.
Arjuna Subject : -
Articles 313 Documents
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Correlation of Blood Creatinine Levels with The Result of Urine Sediment Analysis in Chronic Kidney Failure Patient Siti Zaetun; Rohmi Rohmi; Andi Dwi Oktaviani; Lalu Srigede
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.339

Abstract

Chronic Kidney Failure is a condition caused by a gradual and chronic decline in kidney function. This situation can affect to creatinine levels. Creatinine is the result of endogenous metabolism that is useful for assessing glomerular function, in the event of renal dysfunction then the filtration ability of creatinine will be reduced and serum creatinine will increase. Urine sediment is produced from the results of urine concentration consisting of calcium salts. This concentrated urine comes from the kidneys with a variety of causes, one of which is chronic kidney failure, flows through the ureter to the bladder and continues to the urethra until the urethral. Objective: To find out the correlation of blood creatinine levels with the results of urine sediment analysis in patients with chronic kidney failure. Method: This research is observational analytical with a cross sectional approach to determine the correlation between independent and dependent variables. The sample is taken purposive sampling by selecting a sample based on certain criteria. The collected data is then analyzed using Spearman statistical tests. Results: Examination of creatinine levels in chronic kidney failure patients obtained 6.67 mg/dl. The average urine sediment found erythrocytes as many 2-3/HPF to many/HPF. Conclusion: There is a correlation of blood creatinine levels with the results of urine sediment analysis in patients with chronic kidney failure.
Analysis of the Effect of Taking Anti-Tuberculosis Drugs (ATD) on Blood Glucose Levels and Urine Protein Levels in Tuberculosis Sufferers at the Dasan Tapen Community Health Center, West Lombok Wahyuni, Leni; Agrijanti, Agrijanti; Srigede, Lalu
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 2 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i2.354

Abstract

Tuberculosis is a contagious infectious disease caused by the bacteria Mycobacterium tuberculosis or often called Acid-Fast Bacteria (AFC). Tuberculosis positive patients must undergo treatment by administering category 1 Anti-Tuberculosis Drugs (ATD) for approximately 6-8 months. Anti-Tuberculosis Drugs (ATD) are divided into two phases, namely the intensive phase and the advanced phase. Consuming Anti-Tuberculosis Drugs (ATD) for a long period of time can cause the drug to become toxic in the body, so that it can affect body organs such as the kidneys and pancreas. The aim of this study was to measure blood glucose levels, measure urine protein levels and analyze blood glucose levels and urine protein levels in tuberculosis sufferers who took Anti-Tuberculosis Drugs (ATD). Using analytical observational research methods with a cross sectional research design . Tuberculosis sufferers are checked for blood glucose and urine protein, first and second collection. The results of research on 24 tuberculosis patients who took Anti-Tuberculosis Drugs (ATD) at the Dasan Tapen Community Health Center, West Lombok showed that the blood glucose levels of tuberculosis patients who took intensive phase ATD, namely of the 14 patients, 9 patients experienced an increase. Meanwhile, in the continuation phase, of the 10 patients, there were 6 patients who experienced improvement. The urine protein levels of tuberculosis sufferers who consumed ATD in the intensive and follow-up phase of the first examination were positive (+1) in 1 sample while negative (-) in 23 samples. In the second examination, 2 samples were positive (+1), while 22 samples were negative (-). The conclusion of this study is that there is no relationship between the first and second examination of random blood glucose levels and random urine protein levels.
Isolate Gram Negative Bacteria Resistant Antibiotics Carbapenems in Maternal Urine Pregnant Mahmud, Abbas; Haninggar, Rizki Dyah; Akbar, Fajar
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 2 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i2.397

Abstract

Pregnant women with bacterial uria, more than half have infections with antibiotic- resistant organisms. This pattern of resistance has a real clinical impact because pregnant women with antibiotic-resistant Gram Negative lower urinary tract infections are estimated to be 2-3 times more likely to develop pyelonephritis. Antibiotic resistance is common among pathogenic bacteria that cause urinary tract infections. Enterobacterales are frequently encountered pathogens that cause community-associated infections, such as urinary tract infections. Urinary tract pathogenic bacteria are generally caused by Escherichia coli, Staphylococcus aureus Klebsiella sp, Escherichia coli is a common cause of bacteriuria symptomatic and asymptomatic. In the era of multidrug resistance, appropriate diagnosis and treatment must be given to avoid in pregnant women and prevent antibiotic resistance. Antibiotic-resistant bacteria pose a serious threat to the mother and fetus because it is difficult to obtain safe antibiotics. Increased bacterial resistance of urinary tract pathogens may complicate the selection of appropriate drugs. This study aimed to determine the sensitivity of carbapenem antibiotics to bacterial isolates from the urine of pregnant women. This type of research is descriptive observational research, where the sample used is urine Pregnant. A urine specimen sample is inoculated to in Brain Heart Infusion Broth (BHIB) media, next inoculated to MacConkey agar medium. Isolate bacteria from MacConkey To be done Gram examination and sensitivity test. Sensitivity test use method diffusion the Kirby-Bauer disc uses an antibiotic disc group Carbapenems namely Meropenem (MER) 10ug. Data in the form of mark sensitivity form resistant (R), Intermediate (I) and Sensitive (S) of antibiotic group, participant data processed use SPSS application. This study obtained there were 5 ( 20 %) Gram Negative bacteria that resistant antibiotic meropenem (MER) from group Carbapenems . Conclusion: There is isolate Gram Negative bacteria from urine pregnancy that resistant to antibiotics meropenem from group carbapenemia. Type of group carbapenems the is the antibiotic
Description Of The Enzyme Gamma Glutamyl Transferase In The Patient Hepatitis Amani, Diafa; Kristinawati, Erna; Jiwantoro, Yudha Anggit
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 2 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i2.355

Abstract

Hepatitis caused by the hepatitis virus is still an endemic disease in Indonesia. Most hepatitis viruses are caused by infection with hepatitis viruses A,B,C,D,E. The large number of hepatitis sufferers requires liver function test, one of which is Gamma Glutamyl Transferase (GGT). Objective: This research aims to find out what the gamma glutamyl transferase enzyme looks like in hepatitis sufferers. This research was carried out at the Mataram City Regional Hospital from November to December 2023. The sampling method was carried out using a simple random sampling technique with a total of 25 samples. Result: Examination of Gamma Glutamyl Transferase levels was obtained from 25 patients suffering from hepatitis who tested positive using the enzymatic colometry method. It was found that GGT levels were increased in 5 patients (20%) with an average increase in GGT levels of 185.2 U/L and the average -average normal GGT level is 22.65 U/L. Conclusions: There was an increase in Gamma Glutamyl Transferase levels in 5 patients (20%).
Detection of mecA Genes from Staphylococcus aureus Bacteria in Raw Cow Milk in the Surabaya Regional Farm Putri Lestari, Ajeng Anindhita; Prayekti, Endah Prayekti; Hidayat, Muhammad Taufiq
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 2 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i2.416

Abstract

Milk is a very important food ingredient in meeting nutritional needs and a good growth medium for microorganisms. Staphylococcus aureus bacteria are pathogens that often contaminate milk. Staphylococcus aureus bacteria can cause a disease called mastitis. The acropolis is very susceptible to infections, treatment and prevention need to be done to avoid mastitis. Treatment of mastitis until now still uses antibiotics, improper use of antibiotics can cause Staphylococcus aureus resistance to antibiotics. The mecA gene is the gene responsible for beta-lactam antibiotic resistance. This study aims to determine the presence of the mecA gene in Staphylococcus aureus isolate from raw cow's milk. The samples from this study are 24 samples taken from dairy farms in the Surabaya Region. Isolation of Staphylococcus aureus bacteria by conventional microbiology based on culture properties, Gram staining and biochemical tests. The results of this study showed that there were 5 samples that were positive for Staphylococcus aureus. Molecular detection by PCR of the mecA gene in Staphylococcus aureus was not found in the five Staphylococcus aureus isolates. Based on this study has the result that the sample found Staphylococcus aureus but no mecA gene was found.
Effect Of Incubation Time On Blood Group Changes In Blood Stains Contaminated With Aspergillus Flavus Fakhmi, Shausan Nabila; Khusuma, Ari; Dewi, Lale Budi Kusuma
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 2 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i2.359

Abstract

Introduction: The change of blood group on the spot is when the non-O blood groups, namely A, B and AB, are found to be blood group O (false), this can occur one of them because of the long time of scattered blood (blood stains) found at the scene of the crime, so it can cause blood stains to be contaminated by mycoorganisms. One microorganism known to easily contaminate bloodstains is Aspergillus flavus. This can lead to the degradation of contaminated bloodstains because the contents in the blood can be used by microorganisms as their metabolic material. Objective : This study examines whether there is an effect of incubation time on changes in blood group in blood spots contaminated with Aspergillus flavus, this study uses blood spots of blood group A and blood group B contaminated with Aspergillus flavus. Method : This study was conducted in vitro and is an experimental study using a one-group pretest-posttest design. Blood spots contaminated with Aspergillus flavus were treated with an incubation period of 1 week, 2 weeks, 3 weeks and 4 weeks.  Changes in blood group were then identified using the absorption-elution method. Results : The results of the study as many as 30 units of blood spots of blood group A and blood group B contaminated with Aspergillus flavus did not change the blood group because it can still be identified antigens that match the blood group of the insect at week 4 seen from the occurrence of agglutination. Conclusions : The conclusion that can be drawn from this study is that blood spots of blood groups A and B contaminated with Aspergillus flavus do not change blood groups during incubation times of 1 week, 2 weeks, 3 weeks and 4 weeks or 28 days.
Association Between Diabetes Mellitus Incidence And Cataract Incidence By Gender And Age Muhardi, M. Zul Kifly; Zaitun, Siti; Rohmi, Rohmi; Inayati, Nurul
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 2 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i2.373

Abstract

Diabetes melitus merupakan penyakit jangka panjang yang terjadi ketika pankreas tidak dapat memproduksi cukup insulin atau ketika tubuh tidak dapat menggunakan insulin yang diproduksi secara efektif. Dampak utama diabetes yang tidak terkontrol adalah hiperglikemia atau peningkatan gula darah yang dapat merusak banyak sistem tubuh, terutama saraf dan pembuluh darah. Kadar glukosa yang tinggi pada penderita diabetes melitus (hiperglikemia) juga dapat menyebabkan beberapa komplikasi kesehatan termasuk katarak. Katarak adalah suatu kondisi dimana lensa mata menjadi keruh. Hal ini dapat disebabkan oleh berbagai kondisi seperti penuaan, paparan sinar ultraviolet, diabetes melitus. Katarak merupakan penyebab utama kebutaan di seluruh dunia. Tujuan Penelitian : Untuk mengetahui hubungan kejadian Diabetes Melitus dengan kejadian Katarak Berdasarkan Jenis Kelamin, Usia. Metode Penelitian: Penelitian ini merupakan jenis penelitian Observasional Analitik dengan pendekatan cross-sectional. Teknik pengambilan sampel yang digunakan adalah non-random sampling yaitu purposive sampling yang merupakan teknik yang sering digunakan. Teknik pengambilan sampel yang menggunakan sampel yang dipilih berdasarkan subjektivitas penelitian dan tidak dilakukan secara acak. Hasil: Jumlah sampel dalam penelitian ini adalah 30 responden. 10 responden menderita katarak (10%), 20 responden menderita katarak dan diabetes melitus (20%). Dengan menggunakan uji korelasi spearman diperoleh nilai p = 0,001 (p 1) yang berarti pasien diabetes melitus mempunyai risiko lebih besar untuk menderita katarak dibandingkan dengan pasien tanpa diabetes melitus. Kesimpulan: Diabetes melitus mempunyai hubungan yang signifikan dengan kejadian katarak.
Differences in Erythrocytes Sedimentation Rate Results using Physiological Saline Solution and Phopshate Buffer Saline in Pulmonary Tuberculosis Patients Edijanto, Soebagijo Poegoeh; Layla Agustina, Nurul Ivada; Nugraha, Gilang; Anggraini, Rahayu; Sari, Nathalya Dwi Kartika; Lukiyono, Yauwan Tobing
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 2 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i2.353

Abstract

Tuberkulosis (TB) merupakan penyakit menular yang disebabkan oleh mikroorganisme Mycobacterium tuberkulosis (MTB) pada paru-paru. Terjadi reaksi peradangan oleh sel fagositik dengan menekan bakteri, akibat dari reaksi tersebut terjadi penumpukan cairan eksudat pada alveolus sehingga membentuk granuloma yang berubah menjadi jaringan fibtosa yang dapat mengaktifkan bakteri dan kemudian merusak paru-paru. NaCl 0,9% adalah standar emas dalam Laju Sedimentasi Eritrosit (ESR) yang direkomendasikan oleh Komite Internasional untuk Standardisasi Hematologi (ICSH). Diketahui Saline Phosphate Buffer (PBS) mempunyai tekanan osmolaritas yang sama dengan cairan tubuh, dan bersifat isotonik sehingga mirip dengan NaCl 0,9%. Penelitian ini bertujuan untuk mengetahui perbedaan hasil ESR menggunakan pengencer PBS pH 7,2 dan pH 7,4 pada pasien tuberkulosis di Puskesmas Blega Kabupaten Bangkalan Madura. Sebanyak 30 sampel darah EDTA pasien tuberkulosis. Penelitian ini menggunakan metode eksperimen, dengan desain static group comparation pada perbedaan hasil ESR dengan menggunakan NaCl 0,9% sebagai kelompok kontrol dan PBS pH 7,2 dan pH 7,4 sebagai kelompok perlakuan, diperiksa dengan metode Westergren. . Hasil pengujian dengan menggunakan uji One Way Anova diperoleh p-value 0f 0,997. hasil Laju Sedimentasi Eritrosit menggunakan larutan Phosphate Buffer Saline pH 7,2 dan pH 7,4 dengan NaCl 0,9% pada pasien Tuberkulosis. Kesimpulan penelitian ini adalah hasil pemeriksaan ESR menggunakan larutan pengencer PBS pH 7,2 dan pH 7,4 tidak terdapat perbedaan hasil dengan kelompok kontrol (NaCl 0,9%).
Malaria Density in Peripheral Blood Smear on Positive Cases of Malaria on Residences of Jaro South Kalimantan Nurmansyah, Dian; Khasanah, Maulisa; Wahyuni, Rizka Ayu; Puspawati, Puspawati
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 2 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i2.375

Abstract

Malaria, a severe infectious disease caused by Plasmodium parasites, remains a significant health challenge in endemic regions of Indonesia, particularly in areas with favorable environmental conditions for Anopheles mosquitoes. The liver is a critical organ involved in the early stages of malaria infection. This study investigates malaria density in confirmed malaria cases in Jaro District, South Kalimantan. The aims of this study was to determine the parasitemia density in individuals diagnosed with malaria in the Jaro District, contributing to the understanding of malaria severity in this region. A descriptive survey approach was employed to measure malaria density in 15 respondents with confirmed Plasmodium falciparum infections. Total sampling was used, and blood specimens were collected and analyzed in the clinical pathology laboratory of RS Badaruddin Kasim. Blood smears were stained using 3% Giemsa stain, and parasitemia density was determined microscopically following the Ministry of Health's 2012 guidelines. The study identified varying levels of parasitemia density among the respondents: 33.3% had a low parasitemia density (+), 20% had moderate densities (++ and +++), and 26.7% exhibited high density (++++). The highest proportion of cases showed a low parasitemia density. The analysis of malaria density in the Jaro District reveals significant variability in parasitemia levels among patients. This variability highlights the importance of continuous monitoring and tailored interventions to manage malaria effectively in endemic regions. Understanding parasitemia density is crucial for assessing disease severity and implementing effective treatment
The Influence Of Urine Sample Type (Morning Urine And 12-Hour Urine) On Nitrates, Leukocytes, And Ph In The Carik Celup Method In Urine Of Patients With Urinary Tract Infection Sholihah, Ica Pasi; Diarti, Maruni Wiwin; Urip, Urip; Resnhaleksmana, Ersandhi
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 2 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i2.429

Abstract

Urinalisa is a diagnostic test used to monitor kidney and urinary tract conditions. In the dipstick method, the most important parameters for urinalisa testing are bacteria, white blood cells, red blood cells, nitrite, and protein. Morning urine and urine collected 12 hours later can affect the testing results for nitrite, white blood cells, and pH in patients with urinary tract infections (UTIs). To determine the effect of urine sample type on the results of nitrite, white blood cells, and pH in patients with urinary tract infections (UTIs) using the dipstick method. This study employed an observational analytical design with a purposive sampling method, selecting patients with UTIs who visited RSUD Kab. Dompu in 2024 based on inclusion and exclusion criteria, resulting in a total sample size of 24. The results of the examination were mostly positive for nitrites and leukocytes, while the pH tended to be normal with morning urine samples. However, the majority were positive for nitrites and leukocytes, while the pH tended to be normal with 12-hour urine samples in UTI sufferers at District Hospital Dompu. There is an influence on examining the types of morning urine samples and 12 hour urine samples on the results of nitrite and leukocytes in UTI sufferers at District Hospital Dompu.

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