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Annytha Ina Rohi Detha
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INDONESIA
JURNAL KAJIAN VETERINER
Published by Universitas Nusa Cendana
ISSN : 23564113     EISSN : 25286021     DOI : https://doi.org/10.35508/jkv
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health Veterinary
Jurnal Kajian Veteriner is a scientific journals was published since May, 2012. This journal used to be sharing information and communication about the result of research at veterinary scoup. Jurnal Kajian Veteriner publish twice a year at Juni and December.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 256 Documents
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Escherichia coli Sensitivity Test on Meat and Liver of Broiler Chicken in Kupang City Toelle, Novianti N.; Yasa, Julita D. M.; Jacob, Jois M.; Moenek, Devi Y. J. A.
JURNAL KAJIAN VETERINER Vol 13 No 1 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i1.20934

Abstract

Penelitian ini bertujuan untuk menguji sensitivitas Escherichia coli yang diisolasi dari daging dan hati ayam broiler yang dijual di Kota Kupang terhadap beberapa antibiotik. Sebanyak 30 isolat E. coli diidentifikasi dan diuji sensitivitasnya menggunakan media Muller-Hinton Agar (MHA) dengan empat jenis antibiotik, yaitu tetrasiklin (30μg), penisilin (10μg), gentamisin (10μg), dan oxasilin (10μg). Kekeruhan bakteri disesuaikan dengan standar McFarland No. 5 (1,5 x 10⁸ CFU/ml). Cakram antibiotik diletakkan pada permukaan media MHA yang telah diinokulasi dengan bakteri dan kemudian diinkubasi pada suhu 37°C selama 24 jam. Zona hambat yang terbentuk diukur dengan jangka sorong dan hasilnya diinterpretasikan sesuai dengan pedoman CLSI (2020). Hasil uji menunjukkan bahwa E. coli paling sensitif terhadap gentamisin (90%), diikuti tetrasiklin (60%), penisilin (30%), dan oxasilin (20%). Uji intermediat menunjukkan hasil pada tetrasiklin dan penisilin masing-masing sebesar 10%. Resistensi tertinggi ditemukan pada oxasilin (80%) dan penisilin (60%), sedangkan gentamisin menunjukkan resistensi terendah (10%). Data diuji statistik menggunakan ANOVA yang dilanjutkan dengan uji berganda Duncan untuk menentukan perbedaan antar kelompok. Hasil penelitian ini menunjukkan pentingnya pemantauan penggunaan antibiotik di peternakan ayam untuk mencegah perkembangan resistensi antibiotik yang lebih luas.
Analisis Fisikokimia dan Aktivitas Antibakteri Madu Hutan Timor terhadap Methicillin-Resistant Staphylococcus aureus Undari, Fajar Okta; Latif, Hadri; Sudarwanto, Mirnawati Bachrum
JURNAL KAJIAN VETERINER Vol 13 No 1 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i1.21448

Abstract

The emergence of antibiotic-resistant bacteria has become a global concern. One of the bacteria that is resistant to several types of antibiotics is Methicillin-Resistant Staphylococcus aureus (MRSA). Honey is known for its nutritional and antibacterial properties. The antibacterial activity of honey is due to several factors such as its physicochemical properties, H2O2, and phytochemicals compounds. Honey could be a promising alternative to overcome the problem of antibiotic resistance. The aim of this research was to determine the physicochemical, H2O2, Pinocembrin, Kaempferol and antimicrobial activity of Timor Forest honey. A total of 7 Timor Forest honey was analyzed to determine water content, water activity, pH, H2O2, Pinocembrin, Kaempferol and antibacterial activity. Moisture content, water activity, and pH were measured using a refractometer, aw-meter, and pH meter, respectively. Hydrogen peroxide content was measured using peroxide strip test. Pinocembrin and Kaempferol contents were measured using HPLC-DAD. The analysis showed that the water content, water activity and pH of Timor Forest honey were 17.52±3.31%, 0.6±0.01, 4.18±0.32, respectively. The content of H2O2, Pinocembrin, and Kaempferol were 109.1±47.1 µmol/L, 2.2±1.9 mg/100 g, 1.7±0.7 mg/100 g, respectively. The antibacterial activity of Timor Forest honey against MRSA was tested using Kirby-Bauer disc diffusion method. Vancomycin was used as positive control. The inhibition zone of Timor Forest honey and vancomycin was not significantly different with an average of 13.93±0.23 mm for Timor Forest honey and 14.33±0.05 mm for vancomycin. Timor forest honey has good physicochemical properties with a relatively high content of Pinocembrin and Kaempferol and has antibacterial activity against MRSA.
Characterisation of E. coli Virulence Factors Causing Diarrhea in Macaca Fascicularis at IPB University Captivity, Bogor, Jawa Barat, Indonesia Rizal, Muhammad Farid; Wibawan, I Wayan Teguh; Pamungkas, Joko; Balia, Roostita L.; Darusman, Huda Shalahudin; Surya, Maryati; Achmad, Yusuf Farid; Sundari, Betty; Soedarso, Yovi Alwi; Sibit, Denniswara; Loe, Fhady Risckhy
JURNAL KAJIAN VETERINER Vol 13 No 2 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i2.20778

Abstract

Diare pada Macaca fascicularis sangat mengganggu proses penangkaran, diare mengurangi kualitas hidup kera, E. coli sering dilaporkan sebagai penyebab utama diare pada beberapa primata non-manusia. Tujuan dari penelitian ini adalah untuk mendeteksi faktor virulensi seperti hemolisin, pola hemaglutinin, dan hidrofobisitas permukaan sel. Penelitian ini menggunakan 30 isolat E. coli dari koleksi pusat penelitian primata IPB University yang diisolasi dari kera ekor panjang yang menderita diare. Faktor virulensi seperti hemolisin, hemaglutinasi, dan agregasi garam dideteksi sesuai protokol standar. Sebanyak 30 isolat M. fascicularis yang menderita diare diuji kemampuannya menyebabkan lisis pada eritrosit, di antara 24 (80%) sampel E. coli menunjukkan α -hemolisin pada agar darah dan 2 (6,67%) sampel menunjukkan γ-hemolisis. Sebanyak 21 (70%) sampel E. coli mampu menghemaglutinasi eritrosit sapi dan 27 (90%) sampel mampu menghemaglutinasi eritrosit kelinci. Sebanyak 30 sampel E. coli tidak mampu menghemaglutinasi eritrosit ayam, kucing, anjing, dan manusia. Sebanyak 2 (6,67%) sampel menunjukkan hasil positif pada uji agregasi garam di antara 30 sampel E. coli dari M. fascicularis dan sisanya 28 (93,33%) sampel tidak menunjukkan hasil positif pada uji agregasi garam. Kesimpulan penelitian adalah Sebanyak 30 isolat E. coli menunjukkan variasi faktor virulensi yang berperan dalam mekanisme diare pada Macaca fascicularis. Terdapat 24 isolat (80%) yang menunjukkan α-hemolisin, 2 isolat (6,67%) menunjukkan γ-hemolisin, dan 4 isolat lainnya (13,33%) tidak menunjukkan aktivitas hemolisis. Selain itu, 29 isolat (96,7%) menunjukkan hemaglutinasi terhadap eritrosit sapi dan/atau kelinci, sedangkan 2 isolat (6,7%) bersifat hidrofobik berdasarkan uji salt aggregation.
Efek Sari Daun Sereh Wangi Terhadap Kualitas Semen Babi Landrace X Duroc Dalam Pengencer Tris-Kuning Telur marabijala, jourvan manasye; Nalley, Wilmientje Marlene; Hine, Thomas Mata; Lawa, Alvrado Bire
JURNAL KAJIAN VETERINER Vol 13 No 2 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i2.21196

Abstract

The purpose of this research is to evaluate the level of lemongrass leaf extract added to Tris-egg yolk diluent to ensure that the spermatozoa quality of landrace x duroc crossbred boars is maintained during storage. In this study, fresh semen was used from 1.5 yearsold landrace x duroc crossbred boars that were in healthy condition and had been trained to collect semen. To conduct this research, completely randomized design was used, consisting of six treatments and five replications, namely: T0= Tris-egg yolk, T1= Tris-egg yolk + lemongrass leaf extract 0,5%, T2= Tris-egg yolk + lemongrass leaf extract 1,0%, T3= Tris-egg yolk + lemongrass leaf extract 1,5%, T4= Tris-egg yolk + lemongrass leaf extract 2,0%, T5= Tris-egg yolk + lemongrass leaf extract 2,5%. After dilution, the semen was stored in a styrofoam box at a temperature of 18-20°C. Four variables were tested: motility, viability, abnormality, and sperm survival. These variables were analyzed using a variability test and Duncan's analysis. The findings revealed that the spermatozoa quality of treatment P2 at the 60th hour of observation displayed a notable distinction. (P<0.05) from other treatments. The motility value was 54.00%, the viability was 64.10%, the abnormality was 5.70%, and the sperm survival was 67.68 hours. This study found that adding lemongrass leaf extract to Tris-egg yolk diluent at a level of 1.0% improved the quality of landrace x duroc crossbred boars spermatozoa.
Artikel Pemberian Oligosakarida Madu Alam Sebagai Prebiotik dalam Air Minum dan Pengaruhnya terhadap Performa dan Kesehatan Ayam Pedaging Daud, Muhammad; Yaman, Muha Aman; Zulfan, Zulfan; Armia, Yasser; Wati, Tiara
JURNAL KAJIAN VETERINER Vol 13 No 2 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i2.21272

Abstract

One of the obstacles often faced in broiler farming is the decline in performance and disease attacks. Efforts that need to be made to improve health and prevent disease attacks are by increasing non-specific immune responses through the provision of feed additives such as prebiotics. Prebiotics are non-digestible compounds and function as a food source for good microorganisms in the digestive tract. One such source is natural honey which contains oligosaccharides so that it acts as a prebiotic. The purpose of the study was to evaluate the provision of drinking water containing natural honey oligosaccharides to improve the performance and health of broiler. The research was conducted experementally using a completely randomized design (CRD) consisting of four treatments and the replications. The research treatments included: P0:control, P1:0.5% natural honey oligosaccharides (v/w), P2:1% natural honey oligosaccharides (v/w), and P3:1.5% natural honey oligosaccharides (v/w). The research variables include: ration consumption, body weight gain, final body weight, ration conversion, ration efficiency, drinking water consumption, mortality, feces shape and feces color. Data were analyzed using analysis of variance (ANOVA), followed by Duncan’s multiple range test. The results indicated that ration consumption, body weight gain, final body weight and ration efficiency, drinking water consumption, and health increased in the treatment containing natural honey oligosaccharides and decreased in the ration conversion and mortality of broiler relative to the control. The study concluded that the provision of drinking water containing honey oligosaccharides as prebiotic of 0.5 to 1.5% improved both the performance and health status of broiler.
Perbandingan Teknik Ovariektomi dan Ovariohisterektomi pada Anjing Lokal Indonesia Siswandi, Riki; Soehartono, R. Harry; Kale, Nadya Daramuli
JURNAL KAJIAN VETERINER Vol 13 No 2 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i2.21273

Abstract

Gonadectomy is a common procedure to control dog populations and prevent reproductive diseases. Ovariectomy (OVE) and ovariohysterectomy (OH) are the primary techniques, although their relative efficiency remains under debate. This study compared both methods based on hematological parameters, surgical duration, blood loss, wound healing, progesterone levels, body weight, and postoperative behavior in female dogs. This experiment were using 14 female domestic dogs were divided into OVE and OH groups. Surgeries were performed under general anesthesia, and outcomes were analyzed statistically. Postoperatively, hematocrit and erythrocyte counts significantly decreased in OH group (p<0.05), while white blood cell counts were higher than in OVE (p<0.05), indicating stronger inflammatory response. OVE had a shorter surgical time (56.18 ± 7.10 min vs. 84.41 ± 24.28 min), lower blood loss (4.91 ± 2.60 g vs. 46.02 ± 39.06 g), and faster wound healing (4.57 ± 0.76 days vs. 5.91 ± 1.24 days) (p<0.05). There was no significant differencs in progesterone level one month post surgery between the OVE and OH groups (0.67 ± 0.30 ng/mL and 0.48 ± 0.29 ng/mL, respectively). Likewise, no significant difference were observed in body weight gain, as well as in grooming, sleeping, drinking, exploration and play behaviours between the groups after nine weeks of observation. In conclusion, both techniques effectively reduce plasma progesterone concentration and support body weight gain, but OVE offers greater surgical efficiency, faster recovery, and fewer hematological alterations, highlighting its potential as the optimal technique for sterilizing.
Analisis Aktivitas Nitrifikasi dari Bakteri yang Diisolasi dari Rumah Burung Walet Menggunakan Metode Spektrofotometri Palestin, Palestin; Gusti Ningrum, Siti; Wardhani, Hana Cipka Pramuda; Sutriatmaja, Dyan Nugrahadi; Winda, Charisma Putri
JURNAL KAJIAN VETERINER Vol 13 No 2 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i2.21335

Abstract

Nitrifying bacteria are essential in the nitrogen cycle, turning ammonia into nitrite and nitrate, which greatly contributes to the contamination of edible bird's nests in swiftlet houses. This study aims to evaluate the nitrifying activity of bacteria isolated from swiftlet housing environments in Central Kalimantan, Indonesia. A total of 16 environmental pooling samples were obtained from four different environmental sources within the swiftlet houses: faeces (n=4), pond water (n=4), edible bird’s nests (n=4), and soil (n=4). The nitrifying activity of each isolate was examined by measuring nitrite production using a spectrophotometric method and ammonium consumption using a commercial ammonium test kit. The main criteria investigated were the ability of the bacteria to oxidize ammonia to nitrite and their ability to result in ammonium reduction. The findings indicated that all 16 isolates were able to generate nitrite within a range of 4.8 to 24.7 ppm and showed considerable ammonium consumption. Among the sources, the bacteria from soil exhibited higher nitrifying activity compared to those from soil, pond water and bird’s nests. The findings indicate that the swiftlet house environment harbors diverse and active nitrifying bacteria. Furthermore, spectrophotometry method can be used as an initial detection for nitrifying bacteria.
Ectoparasite Identification in Juvenile Koi Fish (Cyprinus rubrofuscus) in Tanjung Bunga Lake, Makassar City Hartanti, A. Paradiba Nabila; Azis, Hasni Yulianti; Kholilulloh, Zainal Abidin; Raharjo, Hartanto Mulyo; Nurdin, Muhammad Ardiansyah; Mursalim, Muhammad Fadhlullah
JURNAL KAJIAN VETERINER Vol 13 No 2 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i2.22258

Abstract

Keberhasilan budidaya ikan koi sangat bergantung pada produksi stok anakan ikan koi yang sehat dan berkualitas tinggi. Namun, berbagai tantangan, termasuk infestasi ektoparasit, dapat berdampak signifikan pada kesehatan dan pertumbuhan populasi ikan koi. Penelitian ini bertujuan untuk mengidentifikasi ektoparasit yang menyerang ikan koi muda dan menilai kualitas air di Danau Tanjung Bunga, Kota Makassar. Sebanyak 60 sampel ikan koi muda dari tiga titik keramba diperiksa melalui metode scraping, kemudian pewarnaan cacing parasit dilakukan dengan menggunakan pewarnaan semichon’s acetocarmine. Hasil penelitian menunjukkan dominansi Dactylogyrus sp. (52,6%), Trichodina sp. (26,3%), dan Myxobolus sp. (21%). Selain itu, analisis kualitas air menunjukkan konsentrasi oksigen terlarut (DO) di bawah 5 mg/L, yang menunjukkan kondisi lingkungan yang kurang optimal. Penelitian ini menyoroti dominansi infeksi ektoparasit pada ikan koi muda, dengan Dactylogyrus sp. sebagai parasit yang paling dominan, hal ini menunjukkan bahwa perlunya strategi pengelolaan yang lebih baik dalam budidaya ikan koi.
Pengaruh Pemberian Ivermectin Terhadap Perubahan Sel Darah Putih pada Kambing Kacang yang Dipelihara Secara Intensif Leba Tagu Bore, Christvando Rendi; Datta, Frans Umbu; Ndaong, Nemay Anggadewi; Wuhan, Yustinus Oswin Primajuni
JURNAL KAJIAN VETERINER Vol 13 No 2 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i2.22628

Abstract

This study aims to determine the effect of Ivermectin on the differential picture of white blood cells of pea goats before and after endoparasite treatment in a semi-intensive rearing system. The study used 11 pea goats placed in individual pens with a platform and fed with KGP 709 concentrate. Ivermectin was given at a dose of 2.5 ml/head. Tools used included a microscope, EDTA tube, syringe, centrifuge, glass slide and cover, and dropper pipette. Parameters observed were white blood cell profile and gastrointestinal parasite infection based on faecal examination. Data were analysed using One Way ANOVA and presented descriptively. Faecal examination results showed gastrointestinal parasite infection with dominant species Haemonchus contortus (5, TPG 1-33), Trichuris spp. (3, maximum TPG 11), Strongyloides spp. (4, maximum TPG 61), Eimeria spp. (6, mild to severe infection), and Cystotoma spp. (5). Ivermectin administration significantly affected the percentage of neutrophils, eosinophils and basophils (p0.05), indicating that Ivermectin did not affect all white blood cell types equally. In conclusion, this study showed that pea goats were infected with gastrointestinal parasites with a predominance of Haemonchus contortus, Trichuris spp., Strongyloides spp., Eimeria spp. and Cystotoma spp. Ivermectin administration was shown to affect neutrophils, eosinophils and basophils, reflecting the immune response to parasitic infection.
The Pattern of Biological Character, Morphometry, and Genetic Information of Trypanosoma evansi Isolated from Bogor District, Indonesia Nugraha, Arifin Budiman; cahyaningsih, umi
JURNAL KAJIAN VETERINER Vol 13 No 2 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i2.24299

Abstract

Trypanosoma evansi (T. evansi) merupakan penyebab utama trypanosomiasis pada hewan ternak. Penelitian ini dilakukan untuk menggambarkan variasi virulensi, morfometri, dan karakter genetik empat isolat T. evansi yang berasal dari sapi dan kerbau di Kabupaten Bogor, Jawa Barat. Seluruh isolat diperbanyak pada mencit untuk mengamati periode prepatent, perkembangan parasitemia, ciri morfologi, serta hubungan genetiknya. Hasil penelitian menunjukkan adanya tiga pola virulensi. Isolat dengan virulensi sedang berasal dari sapi dan kerbau menyebabkan kematian mencit pada hari ke-14 pascainfeksi. Dua isolat lain dari sapi menunjukkan virulensi rendah (kematian pada hari ke-24) dan virulensi tinggi (hari ke-4). Perbedaan ukuran tubuh juga terlihat jelas, masing-masing 22,05 µm pada isolat virulensi rendah, 24,1 µm pada virulensi sedang, dan 24,6 µm pada virulensi tinggi. Analisis sekuensing menunjukkan bahwa isolat dengan virulensi rendah dan sedang berada dalam kelompok yang sama, sedangkan isolat virulensi tinggi berkelompok dengan isolat dari Thailand dan Tiongkok. Studi ini menunjukkan bahwa isolat T. evansi di lapangan memiliki keragaman biologis dan genetik yang cukup lebar, sehingga pemantauan terhadap penyebarannya tetap diperlukan, termasuk pada isolat yang tampak kurang virulen.

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