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Fitrah al anshori
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INDONESIA
Jurnal Biogenerasi
Published by Universitas Cokroaminoto Palopo
ISSN : 25795163     EISSN : 25797085     DOI : -
Core Subject : Education,
Education Languange, Linguistic, Communication & Media Other
Biogenerasi focuses on the publication of the results of biology education,Biology learning model, Biology learning media, Biology Education Development, The article published on the internal and external academic community Universitas Cokroaminoto Palopo especially in Biology Education.
Arjuna Subject : Umum - Umum
Articles 844 Documents
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PENGEMBANGAN E-LKPD BERBASIS HOTS (Higher Order Thingking Skills) PADA PEMBELAJARAN LINGKUNGAN PESISIR DI SMA NEGERI 2 TILAMUTA Ramin Tanu, Wahyuni; Nusantari, Elya; Sidik Katili, Abubakar; Utina, Ramli; Mustaqimah, Nur
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5882

Abstract

This study aims to develop an Electronic Student Worksheet (E-LKPD) based on Higher Order Thinking Skills (HOTS) using a Problem Based Learning (PBL) approach on coastal environment material at SMA Negeri 2 Tilamuta. The background of this study is the low level of student involvement and the limited availability of teaching materials that foster critical thinking and problem-solving abilities, especially regarding coastal ecosystem topics. The research applies the Research and Development (R&D) method, specifically using the ADDIE model at the development stage, which includes needs analysis, product design, and limited trials involving 26 grade X students and a Biology teacher. Data collection techniques included questionnaires, observations, and interviews, analyzed using both quantitative and qualitative approaches. The results revealed that the developed E-LKPD was highly practical, with teacher response reaching 96.9% and student response 94.5%. Observations also showed the implementation of teacher and student activities reached 100%, 100%, and 95% across three meetings. These findings indicate that the HOTS-based E-LKPD effectively encourages student engagement, enhances conceptual understanding, and creates a meaningful, enjoyable learning experience. Thus, the E-LKPD is suitable to be used as an adaptive, interactive, and contextual learning tool that meets the demands of 21st-century education, especially in enhancing students' critical thinking and problem-solving skills.
PENGEMBANGAN E-MODUL INTERAKTIF BERBASIS KEARIFAN LOKAL SEBAGAI SUMBER BELAJAR LINGKUNGAN PESISIR Husain, Zakaria; Nusantari, Elya; Sidik Katili, Abubakar; Hamidun Susanti, Marini; Mustaqimah , Nur
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5883

Abstract

This study aims to describe the development of an interactive e-module that integrates local wisdom as a learning resource in the coastal environment. The main objective is to help students gain a more meaningful and contextual understanding of coastal ecosystems, while increasing their active involvement in the learning process. This study uses a Research and Development approach with the ADDIE development model. Limited trials were conducted on 26 grade X students at SMA Negeri 2 Tilamuta. Data collection techniques include observation, interviews, questionnaires, and documentation. The research instruments consisted of a needs analysis sheet, validation sheets from material and media experts, and assessment tools from practitioners to evaluate the feasibility of the e-module. The results showed that the interactive e-module received a very positive response in the practicality test, with a percentage of teacher responses of 88% (very good), students of 95% (very good), the implementation of learning 1 of 92% (very good), and learning 2 of 88% (very good). These findings indicate that interactive e-modules based on local wisdom are a feasible and innovative alternative learning resource for use in the classroom..
PENGEMBANGAN MEDIA PEMBELAJARAN WEBSITE BERBASIS GOOGLE SITES SEBAGAI SUMBER BELAJAR MANDIRI PADA MATERI SISTEM PEREDARAN DARAH DI SMA NEGERI 1 TELAGA BIRU Dondo, Bahtiar; Nusantari, Elya; Ibrahim, Mustamin
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5906

Abstract

This study aims to evaluate the validity and practicality of a Google Sites-based website as a self-learning resource for students studying the circulatory system topic. The study employed a Research and Development (R&D) method using the 4D model, which includes the stages of Define, Design, and Develop. However, this study does not involve the stage of Disseminate. Data collection techniques involved expert validation sheets and student response questionnaires. A limited trial was conducted with 30 Grade XI students at SMA Negeri 1 Telaga Biru. The results showed that content validation received a score of 72.22% (Valid), media validation scored 92% (Highly Valid), and student responses reached 85.23% (Highly Feasible). Based on these results, it can be concluded that the Google Sites-based learning media meets the criteria for validity and practicality, making it suitable for supporting the learning process and serving as an effective self-learning resource for students studying the circulatory system at SMA Negeri 1 Telaga Biru.
DESAIN PRIMER GEN EDNRB EKSON 4 DAN OPTIMASI PCR UNTUK ANALISIS MUTASI GEN PADA HIRSCHSPRUNG DISEASE Hidayah, Nurul; Ahda, Yuni; Achyar, Afifatul; Atifah, Yusni
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5954

Abstract

EDNRB gene is a gene located on chromosome 13q22, plays a role in the signaling pathway during the development of the ENS intestinal nerves. Mutations in the EDNRB gene in humans are associated with Hirschsprung Disease (HD). Mutations in the EDNRB gene are the second most common cause after the RET gene in cases of HD with a percentage of 5–10%. To detect mutations, PCR products are needed. Primers are one of the basic components of PCR, while optimization of annealing temperature and optimization of primer concentration are factors in the success of primer attachment to the target gene. This study aims to design EDNRB exon 4 gene primers and determine the optimum annealing temperature and primer concentration to amplify the EDNRB gene. Primer design with Geneious Prime, and good primer criteria are analyzed in silico. Optimization of annealing temperature and primer concentration is carried out with gradient PCR and variations in primer concentration. The results of this study obtained the best primers, namely the forward primer 5'- CAGTAAGTGTGGCCTGAAAG-3' and the reverse primer 5'-GTGGAACCGAAGTGACTAGA-3', which amplified the EDNRB gene exon 4. The optimum PCR conditions for amplification of exon 4 of the EDNRB gene were at an annealing temperature of 57.2 ℃ and at a primer concentration of 0.5 μM.
EKOSISTEM MANGGROVE SUATU ANALISIS BIBLIOMETRIK DAN TINJAUAN LITERATUR Mohamad, Nurdin
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5955

Abstract

Ekosistem mangrove merupakan salah satu ekosistem paling produktif dan vital di wilayah pesisir. Mangrove berperan penting dalam perlindungan pantai, penyerapan karbon, pemeliharaan keanekaragaman hayati, serta penyediaan sumber daya bagi masyarakat sekitar. Tumbuhan ini tersebar luas di daerah tropis dan subtropis, dengan fungsi ekologis yang signifikan dalam mitigasi perubahan iklim melalui kemampuan menyimpan karbon 3-5 kali lebih tinggi dibandingkan hutan daratan. Metode dalam kajian ini adalah Bibliometrika, yakni metode analisis kuantitatif terhadap produksi ilmu pengetahuan, menjadi alat krusial untuk mengeksplorasi dinamika penelitian ini. Pendekatan ini memungkinkan penelusuran pola publikasi, jejaring institusi, serta fokus topik yang dominan atau terabaikan. Hasil analisis dengan menggunakan Vosviewer dan pencarian dengan kata kunci Mangrove diperoleh jumlah artikel secara keseluruhan berjumlah 3047 artikel yang terbit dari tahun 2019 sampai tahun 2024, dimana tahun 2019 berjumlah 458 artikel, tahun 2020 di peroleh 498 artikel, tahun 2021 di peroleh 602 artikel, tahun 2022 berjumlah 578 artikel, tahun 2023 berjumlah 607 artikel dan tahun 2024 berjumlah 304 artikel. Hasil pengolahan data literatur menggunakan analisis Co-occurrence pada sofware Vosviower diperoleh visualisasi yang menggambarkan suatu hubungan kata kunci dengan menggunakan manggrove, dimana terdapat 24 kata kunci yang terbagi dalam 6 clussters yang setiap kolompok memiliki ciri yang diwakili oleh satu warna. Hasil temuan penelitian tentang ekosistem mangrove dengan sumber rujukan yang relevan sebagai berikut: 1) mangrove berperang dalam penyimpanan karbon, 2) Mangrove berperan dalam perlindungan pesisir, 3) menjaga stabilitas iklim.
PENGEMBANGAN MODUL P5 CEGAH ANEMIA DENGAN PANGAN SEHAT SEBAGAI UPAYA DUKUNG CEGAH STUNTING DI SMA NEGERI 1 SUWAWA Hulalata, Wiranda; Nusantari, Elya; Solang, Margaretha; S. Kumaji, Syam; Fajriyani Usman, Nurul
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5974

Abstract

This study aims to develop a module for the Pancasila Student Profile Strengthening Project (P5) with the theme "Prevent Anemia with Healthy Food as an Effort to Support Stunting Prevention" and test its practicality as a teaching tool in project-based learning. This study was conducted at SMA Negeri 1 Suwawa using the Research and Development (R&D) method with the ADDIE approach, which focused on the analysis, design, and development stages. The module was developed to support students' understanding of the relationship between anemia, nutrition, and stunting, as well as to raise awareness of the importance of healthy food consumption. The practicality of the module was tested through a limited trial involving one biology teacher and 30 grade XI students. Data were obtained through questionnaires, observations of learning implementation, and assessments by material and construct experts. The results showed that the P5 module developed had a very high level of practicality. Teacher responses showed an average score of 94%, with a perfect assessment in terms of usability and suitability for students. The students’ responses were also very positive, with an average of 87% covering the aspects of interest, ease of use, and benefits. Observations of the implementation of learning showed an average implementation of 100% in preliminary activities, 92–94% in core activities, and 96% in closing activities. Thus, the module is declared feasible and practical to use in the learning process to support the strengthening of students’ character and knowledge through a health-themed project approach.
PEMERIKSAAN HIV (Human Immunodeficiency Virus) NARAPIDANA YANG DIRUJUK PADA RUMAH SAKIT BHAYANGKARA TK I JAYAPURA PAPUA Taroreh, Jannete Elisabeth; Romadhonni, Tika; Rampa, Ester; Balabuana, Gaspar Bao; Sinaga, Herlando; May, Matilda Bernadece
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.6006

Abstract

This study was entitled HIV Examination in prisoner patients referred to Bhayangkara Hospital TK II Jayapura City. The purpose of this study was to determine HIV infection in serum samples of inmate patients. This type of research is descriptive with a laboratory test approach. This study was conducted for 3 months starting from October 15, 2024 to January 15, 2025. The population and the number of samples used were all inmate patients who conducted examinations at the Bhayangkara TK II Jayapura City Hospital and the number of samples used in this study were 30 samples. The sample used is the serum of inmate patients. The examination method used is the Rapid Diagnostic Test Antibody Method using the Rapid Test tool ViroChek HIV 1/2 brand. The results showed that HIV testing in inmate patients from 30 samples showed Reactive results as many as 3 samples (10%) and 27 (90%) Non Reactive.
EKSTRAK ANTOSIANIN DARI BUAH STROBERI (FRAGARIA X ANANASSA) SEBAGAI PENGGANTI EOSIN PADA JARINGAN HATI TIKUS DENGAN PEWARNAAN HEMATOXYLIN-EOSIN Laila, Nazmy Noor; Mahmud, Dani; Oktari, Anita; Rukhiat, Diat
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.6022

Abstract

Tissue staining is necessary to show the structure and morphology of the tissue, namely Hematoxylin Eosin (H&E) staining. However, eosin is carcinogenic when used in the long term. The aim of this research is expected to develop the use of natural materials as an alternative for Hematoxylin-Eosin histological staining. One of the color pigments that can be obtained from natural materials is the anthocyanin pigment found in strawberries. The anthocyanin extract from strawberries, which is acidic like eosin, will bind to positively charged molecules in the basic cytoplasm. The design of this study uses a simple experimental design (Posttest Only Design). Based on the Mann Whitney U test, a significant result was obtained with a value of sig.<.001 <0.05, indicating a significant difference in the results between the preparations stained with hematoxylin and eosin and those stained with hematoxylin and anthocyanin extract. Thus, it can be concluded that statistically, staining with hematoxylin and anthocyanin extract cannot be used as a substitute for hematoxylin eosin. Nevertheless, the results of staining the preparations using hematoxylin and anthocyanin extract still produce color in the cytoplasm, and are sufficiently contrasting to distinguish between the color of the cytoplasm and the color of the nucleus.
KARATERISTIK MORFOLOGI DAUN TANAMAN TOMAT (SOLANUM LYCOPERSICUM L.) PADA KONDISI LINGKUNGAN YANG BERBEDA Herina, Nandia; Vauzia; Violita; Chatri, Moralita
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.6047

Abstract

Tomato (Solanum Lycopersicum L.) is a horticultural plant from the Solanaceae family that has potential as a vegetable. Tomatoes can grow in various land conditions, both in highlands and lowlands. However, the production of this plant has not been optimal in meeting the needs of the community recently due to various environmental factors. To adapt to different environmental stress conditions, it is important to understand how the plant responds, particularly the morphological responses of tomato leaves in various environmental conditions.This research was conducted from September 2024 to January 2025 in Kamang Magek and the city of Padang. The research method is experimental, comparing the morphological characteristics of tomato leaves, including leaf length and width, petiole length, and the number of leaf veins. The data were analyzed using a t-test at a 5% significance level.The results show that the morphological characteristics of tomato leaves exhibit significant differences in terms of leaf length and width between Kamang Magek and the city of Padang. The average leaf length in Kamang Magek is about 55.5 cm, while in the city of Padang, it is 25.60 cm. The average leaf width in Kamang Magek is approximately 23.30 cm, whereas in the city of Padang, it is 22.95 cm. However, when considering petiole length and the number of leaf veins, no significant differences were observed between the city of Padang and Kamang Magek.
INTERAKSI SINERGIS DALAM KONSORSIUM TERMOFILIK: MENYEIMBANGKAN RASIO KONSENTRASI MIKROBA DALAM PRODUKSI BIOELEKTRIK Alfatimah Azzahra, Balkis; Irdawati; Handayani, Dezi; Hilda Putri, Dwi
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.6048

Abstract

The global urgency for sustainable energy solutions amid climate change and fossil fuel depletion has driven innovation in Microbial Fuel Cells (MFCs). This study investigates the synergistic interactions within a thermophilic microbial consortium (MS9 & MS12) to optimize energy efficiency in MFCs. By varying the inoculum ratios (1:1, 1:2, and 1:3), the research evaluates how microbial population balance impacts bioelectricity production. The MFCs were operated for 24 hours, with voltage measurements recorded every 2 hours. Results revealed that a 1:3 (MS9:MS12) ratio achieved the highest voltage output, peaking at 20 hours before declining, indicating metabolic synergy and efficient substrate utilization. Descriptive analysis highlighted the critical role of cross-feeding mechanisms and functional complementarity between species in enhancing electron transfer. The findings align with modular co-cultivation principles, emphasizing the importance of engineered microbial ratios for stable system performance. This study provides a protocol for designing balanced thermophilic consortia, advancing sustainable bioenergy strategies through optimized microbial interactions.

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