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Borneo Journal of Medical Laboratory Technology
Published by Universitas Muhammadiyah Palangkaraya
ISSN : -     EISSN : 26226111     DOI : https://doi.org/10.33084/bjmlt.v2i2
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology
Borneo Journal of Medical Laboratory Technology is a Scientific Journal managed by Department of Medical Laboratory Technology Faculty of Health Science Universitas Muhammadiyah Palangkaraya and published twice a year (in October and April) by Institute for Researches and Community Services Universitas Muhammadiyah Palangkaraya, contains articles of research and critical-analysis studies in Blood-transfusion Science, Clinical Chemistry, Hematology, Histopathology, Immunology, Microbiology, Parasitology, Toxicology, Food and drink Analysis, Molecular Biology, and other Medical Laboratory aspects.
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 195 Documents
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Pengaruh Lama Penanganan Sampel Sputum Tuberkulosis Terhadap Pemeriksaan Mikroskopis Bakteri Basil Tahan Asam : The Effect Of Tuberculosis Sputum Samples Handling Duration On Microscopic Examination Acid-Resistant Bacillus Bacteria Ina Arbaina
Borneo Journal of Medical Laboratory Technology Vol. 5 No. 1 (2022): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tuberkulosis adalah penyakit infeksi menular yang disebabkan oleh bakteri Basil Tahan Asam (BTA) yaitu Mycobacterium tuberculosis, dapat menyerang paru sehingga disebut tuberkulosis paru (TB paru). Pemeriksaan yang umum untuk diagnosa tuberkulosis adalah pemeriksaan mikroskopis dengan sampel sputum. Di lapangan sering terjadi penundaan pemeriksaan sampel sputum karena satu dan berbagai hal, sehingga sampel sputum harus disimpan pada suhu bervariasi. Tujuan dalam penelitian ini adalah untuk mengetahui pengaruh lama penanganan sampel sputum tuberkulosis pasien terhadap pemeriksaan mikroskopis BTA. Penelitian ini dilakukan dengan metode deskriptif analitik untuk dapat mengetahui pengaruh sampel sputum dari 8 pasien dilakukan dengan dua perlakuan berbeda, yaitu sputum diperiksa segera dan sputum ditunda yang penundaannya dengan disimpan pada suhu -20°C selama 24 jam. Hasil penelitian menunjukkan bahwa ada satu sampel sputum yang mengalami pengurangan hasil dari hasil pemeriksaan sputum segera ke hasil pemeriksaan sputum ditunda 24 jam pada suhu -20°C, sementara 7 sampel lainnya tidak mengalami penambahan atau pengurangan. Hasil uji dengan Uji Wilcoxon didapatkan nilai signifikansi 0,317 (p > 0,05) sehingga disimpulkan menurut uji statistik bahwa tidak ada pengaruh yang signifikan antara hasil pemeriksaan mikroskopis BTA sputum diperiksa segera dan hasil pemeriksaan mikroskopis BTA sputum ditunda 24 jam pada suhu -20°C.
Kontaminasi Escherichia coli pada Air Minum Isi Ulang di Wilayah Ciangsana Menggunakan Uji Most Probable Number (MPN): Escherichia coli Contamination in Drinking Water Refills at Ciangsana Region using Most Probable Number Test Mohammad Maulana Fajar; Muhammad Rizki Kurniawan; Dian Rachma Wijayanti
Borneo Journal of Medical Laboratory Technology Vol. 5 No. 2 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v5i2.4102

Abstract

Water is a basic need for humans. Human life cannot be separated from water, especially the need for drinking water. Water needs have been sourced from groundwater, river water, rainwater, mountain water, and treated seawater. Water that humans need includes clean and healthy useable water for cooking, washing, and bathing, as well as water that is fit for consumption for drinking purposes. The main use of water and very vital for life is the use of drinking water. Drinking water from depot companies for drinking water refills is an industrial business that processes raw water into ready-to-drink water by consumers. This study aims to determine the presence of Escherichia coli contamination in drinking water sold by refilled drinking water depots in the Ciangsana region. The results of the microbiological analysis showed two out of ten samples were positive for coliform fecal, Escherichia coli.
Evaluasi Kadar Kreatinin dan Mikroalbumin dengan HbA1C Suryanata Kesuma; Sresta Azahra; Amalia Diah Suci Rahmah
Borneo Journal of Medical Laboratory Technology Vol. 5 No. 2 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v5i2.4790

Abstract

Diabetes Mellitus (DM) is a metabolic disease caused by high blood glucose levels (hyperglycemia). Hyperglycemia is caused due to lack of insulin secretion in the body. Glycemic control in diabetic patients is seen in HbA1C levels. One of the complications of DM is diabetic nephropathy. Renal function abnormalities associated with diabetic nephropathy are indicated by increased levels of creatinine and microalbumin. This shows that it is necessary to monitor kidney function in DM. Monitoring DM patients with HbA1C levels <6.5% is desirable, with these levels indicating that glycemic control is good. This study aimed to determine the relationship between creatinine and microalbumin levels with HbA1C <6.5% in Diabetes Mellitus patients in Samarinda. This type of research is analytic with a cross-sectional design. Spearman correlation statistical test. The sample of this study was 107 DM patients with HbA1C levels <6.5% who did medical checkups at the Media Farma Clinic, Samarinda. Sampling is done by Total Sampling. HbA1C examination method with turbidimetry. Method of checking creatinine by Jaffe reaction. Method of examination of microalbumin with immunoturbidimetry. All parameters are quality controlled before measurement. The measurement results were validated by a clinical pathologist. The results showed that the correlation of creatinine with HbA1C <6.5% obtained p value = (0.826), microalbumin with HbA1C <6.5% obtained p value = (0.146), and creatinine with microalbumin obtained p value = (0.082). All these results indicate that H0 is accepted and Ha is rejected. This study concludes no significant relationship exists between creatinine and microalbumin values with HbA1C levels <6.5% in Diabetes Mellitus in Samarinda, especially Media Farma Clinic.
Gambaran Hasil Pemeriksaan Aktivitas Enzim Alanine Aminotransferase (ALT) pada Serum Setelah Disimpan Selama 14 Hari: Overview of Alanine Aminotransferase (ALT) Enzyme Activity Assay Result in Serum After Storage for 14 Days Putri Kurniasiwi; Roosmarinto Roosmarinto; Narendra Yoga Hendarta
Borneo Journal of Medical Laboratory Technology Vol. 5 No. 2 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v5i2.4866

Abstract

Storage of serum at 4°C over three days causes a 10% decrease in the enzyme Alanine Aminotransferase (ALT) activity. However, several laboratories store remaining serum samples for one week or even one month after examination, which is not under Good Laboratory Practice (GLP). The serum storage is used to anticipate if there is an examination error. This study aims to describe the results of examining ALT enzyme activity in serum that was immediately checked and stored for 14 days. This type of research is descriptive quantitative using the Pretest-Posttest Design. The sample in this study was human blood serum, with a total sample of 10 patients. Data were analyzed descriptively for all data obtained and presented in tabular form. The average yield of ALT enzyme activity examination before storage and storage on day seven and day 14 was 50.7 U/L, 46.1 U/L, and 40.5 U/L. This study concluded that there was a decrease in the results of examining the activity of the ALT enzyme by 9% on the 7th day and 20% on the 14th day. Therefore, it is suggested that laboratory staff check the sample as soon as possible. If there is a need to repeat the analysis, verify the results or add laboratory tests, samples should be frozen to ensure stability.
Kualitas Media Agar Darah Manusia dan Domba pada Pertumbuhan Streptococcus β hemolyticus: Quality of Human and Sheep Blood Agar Media on the Growth of Streptococcus β hemolytic Mochamad Rizal Maulana; Maseva Wijayanti
Borneo Journal of Medical Laboratory Technology Vol. 5 No. 2 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v5i2.4898

Abstract

Sheep blood agar (ADD) is a growth medium for identifying pathogenic bacteria and is a standard medium for microbiological examination. Some countries experience difficulties multiplying ADD due to several factors, so another alternative is needed: using human blood agar (ADM) as a substitute. This study examines the feasibility of ADM as an alternative medium to replace ADD in growing Streptococcus β hemolyticus. This study used sheep and human blood agar, which were then observed at 24 and 48 hours of incubation to show differences in hemolysis in diameter. At 24 hours, the average hemolytic diameter of ADM did not show hemolysis (0.00mm) and was different from ADD (0.40mm). ,10mm). Based on the above results, in the future, it is necessary to control other variables that might influence, such as Hb and age of erythrocytes, agar thickness, pH used, and planting techniques (strict) to obtain maximum growth results.
Faktor Risiko Kelainan Kadar Eritrosit pada Pengendara Ojek Motor Online di Kawasan Industri Tanjung Mas Kota Semarang: Risk Factors for Abnormal Erythrocyte Levels in Online Motorcycle Taxi Riders in the Tanjung Mas Industrial Area, Semarang City Lilik Setyowatiningsih; Qurrotu A’yuni Auliya; Ririh Jatmi Wikandari
Borneo Journal of Medical Laboratory Technology Vol. 5 No. 2 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v5i2.5099

Abstract

Industrial activities and motor vehicle exhaust gases significantly contribute to air pollution; the longer the exposure to pollutants, the higher the pollutant levels in the blood. Online motorcycle taxi drivers in industrial areas have a high risk of being exposed to pollutants. This study aims to determine the risk factors that affect erythrocyte levels in online motorcycle taxi drivers in the industrial area of ​​Semarang City. The method used is analytic observational with a cross-sectional approach. Of 55 respondents, 41 had normal erythrocyte levels, and 14 had above-normal erythrocyte levels (polycythemia). Chi-square test results on smoking status, educational status, working length, and knowledge did not show a significant relationship (p <0.05) on erythrocyte levels in online motorcycle taxi drivers in industrial areas, Semarang City. The conclusion of this study, there is no significant relationship between smoking status, educational status, length of time working, and knowledge of the erythrocyte levels of online motorcycle taxi drivers in the Tanjung Emas industrial area, Semarang City.
Efektifitas Penggunaan Deterjen Laundry Cair sebagai Agen Deparafinisasi pada Sediaan Ginjal Mencit (Mus musculus): Effectiveness of Using Liquid Laundry Detergent as a Deparaffinizing Agent in Mice Kidney Preparations Faiz Shofa Abdillah; Eko Naning Sofyanita
Borneo Journal of Medical Laboratory Technology Vol. 5 No. 2 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v5i2.5100

Abstract

Deparaffinization is one of the stages of tissue processing, which aims to dissolve paraffin for full-color application. Deparaffinization can only be done by xylol to dissolve paraffin as fat. Liquid laundry detergent has the main component in the form of a surfactant which has the property of removing types of lipids by dissolving and emulsification because xylol has carcinogenic properties, flammable properties, and is relatively expensive. This study aimed to describe the quality of tissue preparations using liquid laundry detergents 1.5%, 3%, and 4.5% as deparaffinized agents and also to describe and compare several concentrations of the most effective laundry detergents as deparaffinized agents. Results from the preparation quality using xylol as a deparaffinized agent by conducting an experimental study with a cross-sectional design showed promising results. The quality of preparations using 1.5% liquid laundry detergent got poor results. The quality of preparations using 3% liquid laundry detergent got poor results. The quality of preparations using 4.5% liquid laundry detergent got good results. Based on the results of the Kurskal Wallis test with a significance value of p <0.05, which indicates that H0 is rejected, the conclusion that can be drawn is that there is an unfavorable difference in results between xylol and liquid laundry detergent as a deparaffinized agent.
Tinjauan Pewarnaan Hemaktosilin-Eosin dan Periodic Acid-Schiff terhadap Kerusakan Hati Mencit yang Diinduksi Aloksan: A Review of Hematoxylin-Eosin and Periodic Acid Schiff Staining to Assess Alloxan-Induced Liver Injury in Mice Nanik Aryani Putri; Erick Khristian; Adang Durachim
Borneo Journal of Medical Laboratory Technology Vol. 5 No. 2 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v5i2.5101

Abstract

The liver is an organ that serves several functions in the body, including glucose metabolism to provide energy to other tissues. Hepatocytes are the primary functional cells of the liver, which also regulate liver glucose release via glucose transport protein-2. Hepatocyte injury could occur from toxic substances and diseases such as Diabetes Mellitus. Alloxan is an organic compound commonly used in diabetes research as a diabetogenic agent. Alloxan causes diabetes through selective inhibition of glucose-stimulated insulin secretion and induced formation of reactive oxygen species (ROS) that promotes pancreatic beta cell necrosis. Alloxan also affects the liver's histological condition, including the hepatocellular structure and glycogen content. HE and PAS are used for evaluating this condition. However, both should be reviewed to evaluate their abilities. Mice were divided into control and test groups, each consisting of 5 mice. The test group was intervein-induced with 25, 50, and 100mg/KgBB alloxan on the second day of arrival. The mice's livers were then taken on the seventh day; tissue processing was carried out to get 20 blocks of mice's livers. Two 5-μm-thick paraffin-embedded sections from each group were stained with hematoxylin-eosin, and periodic acid Schiff, respectively. Mice's liver slides are examined microscopically for the degree of injury and glycogen concentration for further evaluation using ImageJ digital imaging application. This study found that microscopical and ANOVA tests of both staining methods successfully produced significant differences between control and various dose alloxan-induced groups of mice.
Identifikasi Bakteri Pereduksi Logam Pb dalam Bioremediasi Sampel Air Sungai Citarum Menggunakan Analisis Gen 16s rRNA: Identification of Pb Metal Reducting Bacteria in Bioremediation from Citarum River Water Samples Using 16s rRNA Gene Analysis Ni'matul Murtafi'ah; Suci Rizki Nurul Aeni
Borneo Journal of Medical Laboratory Technology Vol. 5 No. 2 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v5i2.5102

Abstract

The bioremediation technique is an effort to remove the heavy metal Pb in samples of Citarum river water. High concentrations of heavy metals harm the environment, so Pb metal-reducing bacteria are needed as a pollution solution. Bioremediation uses bacteria. Bacteria are identified by molecular methods, namely sequencing analysis techniques, because they are more accurate. To determine the nucleotide sequence of Pb metal-reducing bacteria isolates and which bacterial species can reduce Pb metal. The methodology used is primary data collection, namely conducting research directly, namely the stages of bacterial purification through gram staining techniques, pure colonies, then proceeding to the DNA isolation stage. The isolated DNA is then electrophoresed. The electrophoresis results are then purified and proceed to the sequencing stage. The feasibility test results were to obtain pure DNA from the reducing bacteria. The next step was PCR amplification of the 16S rRNA gene of Pb-reducing bacteria. The research results started at the DNA isolation stage and obtained a purity of 1.80. The PCR results were in the form of amplification of the 16S rRNA gene of Pb-reducing bacteria. Electrophoresis obtained the results of DNA bands measuring ± 1400 bp—phylogenetic tree analysis with 99.60% relatedness to Bacillus cereus, which can reduce Pb. Bacillus cereus species can carry out detoxification mechanisms against the toxic effects of Pb metals, such as—biosorption, bioaccumulation, reduction, solubilization, precipitation (formation of extracellular complexes), and methylation.
Identifikasi Bakteri pada Nutrient Agar Plate di Laboratorium Mikrobiologi Universitas Muhammadiyah Palangkaraya: Identification of Bacteria on Nutrient Agar Plate at the Universitas Muhammadiyah Palangkaraya’s Microbiology Laboratory Windya Nazmatur Rahmah; Fera Sartika; Yuda Ednisa Sri Maduren
Borneo Journal of Medical Laboratory Technology Vol. 5 No. 2 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v5i2.5177

Abstract

A laboratory is a place or room carrying out research, learning, and experiment activities equipped with various equipment and materials supporting the work. This study aims to identify bacteria on nutrient agar plates at the Universitas Muhammadiyah Palangkaraya’s Microbiology Laboratory. This study uses observational research with a non-probability sampling technique with a cross-sectional research design. Bacterial samples were taken at several points using nutrient agar plates in the microbiology laboratory room. The results showed that when cultured on Nutrient Agar media in 12 glasses of petri, the results obtained were 100% bacterial growth. Bacteria identified using the gram staining method received 41.7% gram-negative Streptococcus bacteria, 41.7% gram-negative Staphylococcus, and 16.7% gram-positive Staphylococcus.

Page 8 of 20 | Total Record : 195

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