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Borneo Journal of Medical Laboratory Technology
Published by Universitas Muhammadiyah Palangkaraya
ISSN : -     EISSN : 26226111     DOI : https://doi.org/10.33084/bjmlt.v2i2
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology
Borneo Journal of Medical Laboratory Technology is a Scientific Journal managed by Department of Medical Laboratory Technology Faculty of Health Science Universitas Muhammadiyah Palangkaraya and published twice a year (in October and April) by Institute for Researches and Community Services Universitas Muhammadiyah Palangkaraya, contains articles of research and critical-analysis studies in Blood-transfusion Science, Clinical Chemistry, Hematology, Histopathology, Immunology, Microbiology, Parasitology, Toxicology, Food and drink Analysis, Molecular Biology, and other Medical Laboratory aspects.
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 196 Documents
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Perbandingan Hasil Hitung Jumlah Sel Darah Spesimen Darah Vena EDTA Menggunakan Metode Manual Dan Otomatis: Comparison Of Result Counting Blood Cell Number Of EDTA Vena Blood Specimen Using Manual And Automatic Methods Kurniasih, Erin; Astuti, Tri Dyah
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 2 (2024): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i2.5876

Abstract

Pemeriksaan hematologi merupakan pemeriksaan darah yang paling awal untuk mengetahui diagnosis penyakit. Pemeriksaan hematologi yang lazim digunakan yaitu pemeriksaan hitung jumlah sel eritrosit, leukosit, trombosit. Pemeriksaan hematologi ini bisa menggunakan metode manual dan metode otomatis. Dengan metode perhitungan yang berbeda tentunya akan menimbulkan variasi hasil perhitungan sel darah. Tujuan dari penelitian ini yaitu untuk mengetahui ada tidaknya perbedaan hasil hitung jumlah sel darah spesimen darah vena EDTA menggunakan metode manual dan otomatis. Metode penelitian yang digunakan adalah tinjauan literatur, pencarian data dilakukan dengan metode PICO dari database Google Scholar, Wiley Online Library, dan PubMed. Hasil penelitian ini ditemukan 11 jurnal yang sesuai dengan topik dan kriteria penelitian. Berdasarkan uji Independent sample t-test didapatkan nilai signifikasi sel eritrosit sebesar 0.0253 (>0.05), nilai signifikasi sel leukosit sebesar 0.242 (>0.05), dan nilai signifikan sel trombosit sebasar 0.273 (>0.05) yang artinya tidak ada perbedaan yang signifikan pada hitung jumlah sel darah dengan metode manual dan otomatis. Bagi peneliti selanjutnya disarankan untuk melakukan penelitian secara langsung dengan metode manual maupun otomatis menggunakan sampel darah vena EDTA dari pupulasi yang beragam, termasuk kondisi pasien dan kondisi klinis yang berbeda untuk melihat perbedaan terhadap hasil pemeriksaan hitung sel darah dan menambah literatur yang lebih banyak lagi supaya dapat mengembangkan penelitian ini.
Gambaran Kadar Glukosa Darah Puasa Pada Lansia Di UPT PSTW Khusnul Khotimah Dinas Sosial Provinsi Riau: Glucose levels in fasting blood in the Elderly at the UPT PSTW Khusnul Khotimah in the RIAU province Yusrita, Eli; Aryesta, Desty Agnes; Juariah, Siti
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 2 (2024): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i2.5932

Abstract

Diabetes Melitus (DM) termasuk salah satu penyakit tidak menular yang telah menjadi masalah serius bagi kesehatan masyarakat di Indonesia maupun di dunia. Indonesia menduduki peringkat ke 7 di dunia dengan penderita diabetes sebanyak 10,7 juta pada tahun 2019 dan jumlah tersebut diperkirakan akan meningkat menjadi 13,7 juta pada tahun 2030 dan 16,6 juta pada tahun 2045 (1,2). Komplikasi yang terjadi pada penderita DM adalah karena kerusakan dan kematian sel yang disebabkan peningkatan stress oksidatif dan aktivitas mediator inflamasi temasuk interleukin-6 (Il-6). Pemberian obat hipoglikemi oral pada pasien DM tidak menunjukkan perbaikan pada stress oksidatif. Oleh karena itu, diperlukan terapi tambahan untuk pasien DM yang dapat menurunkan stress oksidatif dan mediator inflamasi serta mendukung kerja antioksidan endogen. Daun bandotan merupakan tanaman yang mengandung antioksidan dan digunakan sebagai obat tradisional untuk pasien DM. Tujuan dari penelitian ini adalah untuk mengetahui kadar guloksa darah sebetlum terapi ekstrak daun bandotan. Penelitian ini menggunakan tikus wistar jantan yang diinduksi dengan streptozotocin (stz) untuk menjadi DM tipe 2. Kemudian diperiksa kadar glukosa darah pada hari ke lima setelah induksi stz. Hasil menunjukkan secara keseluruhan kadar glukosa darah telah mencapai lebih dari 200 mg/dl. Hal tersebut menunjukkan bahwa tikus telah mengalami hiperglikemi dan bisa dilanjutkan untuk perlakuan terapi.
Gambaran Profil Salmonella Sp. Pada Getuk: Profile Of Salmonella sp. In Getuk Suhandayati, Elviana; Hermawati, Andyanita Hanif; Hariyanto, Hariyanto; Cahyariza, Nosa Ika
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 2 (2024): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i2.5996

Abstract

Getuk merupakan makanan ringan yang terbuat dengan bahan utama ketela pohon atau singkong. Bakteri yang menyebabkan diare atau foodborne diease masuk melalui berbagai cara yaitu oral, lingkungan yang tercemar, makanan, dan lain-lain sehingga kondisi seperti ini sangatlah tergantung dengan pedagang, bagaimana pedagang tersebut tetap mempertahankan kehigienisan makanan yang dijualnya agar tidak terkontaminasi. Tujuan dari penelitian ini adalah untuk mengidentifikasi bakteri Salmonella sp. pada makanan tradisional getuk yang dijual di Pasar Ngunut. Penelitian ini merupakan penelitian deskriptif. Yang dilaksanakan pada 24 sampai 29 Mei 2018 di Laboratorium Mikrobiologi DIII Teknologi Laboratorium Medis STIKes Hutama Abdi Husada Tulungagung. Populasi pada penelitian ini adalah semua getuk yang dijual di Pasar Ngunut, teknik sampling pada penelitian ini adalah total sampling, yang diperoleh sebanyak 6 sampel makanan tradisional getuk. Instrumen yang digunakan diantaranya adalah tabung steril, pipet ukur, incubator, plate, media agar plate, aquadest, oven dan peralatan laboratorium yang dibutuhkan. Hasil penelitian diperoleh bahwa dari 6 sampel, 3 sampel positif bakteri Salmonella dan 3 sampel tidak ditemukan bakteri Salmonella sp. Hasil penelitian selanjutnya dianalisis secara diskriptif. Dari hasil penelitian yang telah dilakukan dapat disimpulkan bahwa sampel teridentifikasi bakteri Salmonella sp pada makanan tradisional getuk yang dijual di Pasar Ngunut Tulungagung dengan menggunakan media Selenite Broth sebagai pemupuk, SSA sebagai media selektif, KIA untuk melihat Gas dan H2S, MIU untuk meliat urea dan motilitas bakteri, serta pengecatan Gram untuk melihat Gram Negatip batang
Uji Stabilitas Pooled Sera Yang Disimpan Di Suhu 2 - 8ºc Pada Pemeriksaan Albumin: The Pooled Sera Stability Test is Stored at a Temperature of 2 - 8ºc on Albumin Ramdhani, Fitria Hariati; Purbayanti, Dwi; Astuti, Putri
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6073

Abstract

The health laboratory is one of the health services for the community in helping to establish a diagnosis, monitoring treatment results, and determining the prognosis of a disease. Control serum is very important for assessing performance in the laboratory, including instrument and reagent quality. Some laboratories use pooled serum as control serum for daily examination. Albumin is the most abundant plasma protein in the human body, which is about 55-60% and the total normal serum protein level is 3.8-5.0%. This study aims to test the stability of pooled sera on examination of albumin stored in the refrigerator at a temperature of 2 - 8ºC for 2 weeks. This research method uses observational research methods with precision data analysis techniques (accuracy). The results of the study of examining albumin levels using the Bromcresol Green (BCG) method obtained serum stability results on day 1 to day 5, where the CV value from the albumin level examination entered the maximum CV value on albumin, which was 6. The CV value for the 5th day covers, day 1 to day 2 = 1.99. Day 1 to day 3 = 2.17. Day 1 to day 4 = 4.27. And day 1 to day 5 = 4.84. On day 6 to day 14 and so on, the serum is found to be unstable and the value does not enter the maximum CV value.
Imunogenesitas Protein Pilli Salmonella Typhi Pada Mencit Balb/C Dengan Mengukur Kadar TNF-α: Imunogenesity Of Protein Pilli Salmonella typhi in BALB/c Mice by Measuring TNF-α Controls Hidayani, Al; Darmawati, Sri
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6074

Abstract

Typhoid fever, often called typhoid disease, is caused by Salmonella enterica subspecies enterica serevoar typhi (Salmonella typhi). The virulence of S.typhi bacteria depends on its ability to invade host cells consisting of several components such as cell wall antigens, flagellum antigens, Vi antigens and pilli antigens. Pilli is one of the virulence factors of S.typhi bacteria, which functions as a tool for adhesion or interaction between bacteria. The study was conducted to determine how the immunogenicity of pilli protein of S.typhi bacteria isolate BA07.4 was immunized in Balb/C mice at varying doses (2-6μg) by measuring TNF- α. The analysis carried out obtained the optimum results of TNF-α levels at a dose of 3μg with an average level of 62.152 pg / ml. In the research conducted, it was found that S.typhi pilli protein can induce specific antibodies against pilli antigens. This shows that the pilli protein is immunogenic, can cause an immune response and can induce antibodies that can recognize the immunized pilli protein sub-unit.
Aktivitas Antibakteri Kombinasi Ekstrak Biji Manjakani (Quercus infectoria) Dan Daun Sirih Merah (Piper crocatum) Terhadap Pertumbuhan Bakteri Klebsiella pneumoniae: Antibacterial Activity Combination of Manjakani Seed Extract (Quercus infectoria) and Red Betel Leaf (Piper crocatum) Against the Growth of Klebsiella pneumoniae. Ashari, Ayu; Harlita, Tiara Dini; Aina, Ganea Qorry
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6075

Abstract

Potential plants found in Indonesia that can be used for the treatment of urinary tract infections include: Manjakani seeds (Quercus infectoria) and red betel leaves (Piper crocatum). Manjakani seeds are pharmacologically claimed to have biological activities such as anti-inflammatory, antibacterial and antifungal. Red betel leaf has the potential as an antibacterial in the treatment of infectious diseases. The purpose of this study was to determine the antibacterial activity of the combination of manjakani seed extract and red betel leaf in inhibiting the growth of Klebsiella pneumoniae bacteria. The type of research used istrue experiment study with a completely randomized design (CRD) with three factors, namely the type of solvent n-hexane, ethyl acetate, ethanol 96%, ratio (1:1,1:2,2:1) and concentration (75%,100%) with each treatment being repeated twice. This research was conducted in the microbiology laboratory, Labkes, East Kalimantan. The population in this study is the manjakani plant and red betel plant and tested using the agar diffusion technique method Kirby Bauer with positive control (Ciprofloxacin) and negative control (aquadest). Data analysis used is One Way ANOVA and advanced test methods Duncan with a significance level of 0,05. The results obtained showed that there was an effect of antibacterial activity of Manjakani seed extract and red betel leaf on growth K. pneumoniae. The most effective concentration was found in ethanol 96% extract, (2:1 combination of 100% concentration) of 13,97 mm and an effectiveness of 55,74% with a positive control ciprofloxacin 5µg of 25,06 mm. It can be concluded that the combined extract of manjakani seeds and red betel leaves has antibacterial activity against bacteria Klebsiella pneumoniae,but the potential is still low of ciprofloxacin.
Optimasi Konsentrasi Primer Dan Suhu Annealing Dalam Mendeteksi Gen Blaz Pada Bakteri Methicillin Resistant Staphylococcus Aureus Di Udara: Optimization of Primary Concentration and Annealing Temperature in Detecting Blaz Gene in Airborne Methicillin-resistant Staphylococcus aureus Bacteria Mardiana, Anisah; Dhanti, Kurnia Ritma; Kurniawan, Kurniawan; Sulistyowati, Retno
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6076

Abstract

Staphylococcus aureus is a species of bacteria that is often identified as a source of infection and has increased the number of cases of resistance. Resistance to Penicillin-class antibiotics in S. aureus is associated with plasmids containing the blah-Z gene encoding ß-Lactamase. Some factors that can be modified to obtain optimal test results in PCR are annealing temperature and primary concentration. This study aims to identify the primary concentration and annealing temperature that produce PCR products with the best band in detecting the blaZ gene in Metichilin Resistant Staphylococcus aureus (MRSA) bacteria. The research design used used experimental methods. The results of the study obtained were PCR products at primary concentrations of 10 μM were clearly visible, thick and clean, compared to primary concentrations of 5 μM. Annealing temperature with the best band results at temperatures of 61.8 °C and 58.7°C. This study concluded the primary concentration to obtain a clearly visible band result of 10 μM. The annealing temperature of 61.8°C produced the best band in the sample code R.PG. U4, R.V.U2, and R.151.U2 temperatures. While the sample code R.IGD. U5 band results best at annealing temperature 58.7°C.
Hubungan Jumlah Monosit Dan Nilai Laju Endap Darah (LED) Terhadap Lama Pengobatan Pasien Tuberculosis : Correlation Of Monocyte Number And Erythrocyte Sedimentation Rate (Esr) Value Against To Duration Of Treatment In Tuberculosis Patients Kadarwati, Ari; Sukeksi, Andri; Putri, Gela Setya Ayu
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6077

Abstract

Tuberculosis (TBC) is a bacterial infection caused by Mycobacterium tuberculosis. The duration of TBC treatment is routinely 6 months; otherwise, resistance will develop. TBC can increase leukocytes, including monocytosis as an inflammatory response and an elevated ESR. The aim of this study was to determine the relationship between the number of monocytes and the ESR value on the duration of tuberculosis treatment in patients at the Respira Lung Hospital, Yogyakarta City. The study was conducted in June and July 2023 at the Respira Pulmonary Hospital in Yogyakarta City. The study sample was 32 tuberculosis patients and blood was drawn to determine the monocyte number and ESR value. The results showed that the average number of monocytes was 7% (normal), the ESR value was 41.59 mm/hour (high) with the average duration of tuberculosis treatment for respondents was 3 months. Spearman test obtained Sig p<0.05. This study concluded that there is a correlation between the number of monocytes and the ESR value on the duration of TB treatment in patients at the Respira Lung Hospital in Yogyakarta City, with the direction of the relationship reversed.
Pemeriksaan Pediculus Humanus Capitis Pada Anak Sekolah Dasar Di Pesantren Hidayatullah Kota Palangka Raya Tahun 2023 : Pediculus Humanus Capitis In Examination in Primary School Children at Hidayatullah Boarding School Palangka Raya In 2023 Nanda, Putri; Sartika, Fera; Ramadhani, Fitria Hariati; Rahmah, Windya Nazmatur
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6080

Abstract

Head lice (Pediculus humanus capitis) are parasites that live on the hair and scalp of humans by sucking blood from the human scalp. The transmission of head lice can occur directly (hair to hair) and indirectly through shared accessories. A pesantren is one of the places where several people gather in the same location, and this is a primary factor in the spread of Pediculus humanus capitis infection. The research objective is to determine the number of children infected with Pediculus humanus capitis. The research method used is descriptive. The sample in this study consisted of 14 individuals taken using total sampling technique. The research results showed that there were 4 students (29%) infected with Pediculus humanus capitis at Pesantren Hidayatullah in Palangka Raya City, and 10 students (71%) were not infected with Pediculus humanus capitis. The positive results are believed to be due to the habit of using hair accessories such as shared hair combs, which increase the occurrence of Pediculus humanus capitis infection.
Potensi Ikan Seluang (Rasbora spp.) Dan Ikan Lais (Kryptopterus spp.) Dalam Meningkatkan Performa Tikus Wistar (Rattus Norvegicus) Malnutrisi: The Potential of Seluang Fish (Rasbora spp.) and Lais Fish (Kryptopterus spp.) in Improving the Performance of Malnourished Wistar Rats (Rattus norvegicus) Melatiasha, Keniashinta; Salsabil, Sarah; Berliana, Anggia Asha; Lumingkewas, Gloria; Sayyidinnor, Muhammad Alif; Yuliani, Ni Nyoman Sri
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6081

Abstract

Malnutrition caused by a lack of nutritional intake in children can have a negative impact on physical growth, cognitive function development and immune levels. Malnutrition conditions which are persistently allowed without special intensive and consistent treatment will result in stunting. The efforts that can be made to overcome the incidence of stunting are by fulfilling complex nutrition which can come from various sources, one of which is by consuming seluang fish and lais fish which have high calorie, protein and fat content since they have the potency to improve nutrition in children, especially children who suffer from stunting. The aim of this research was to see the potential of seluang and lais fish in improving performance in Wistar rats induced by malnutrition. This research type is a true experimental research with a pretest-posttest with control group design using 25 male white rats (Rattus norvegicus) Wistar strain aged 2-4 weeks which will be divided into 5 groups, namely K(+), K(-) , P1, P2, P3. The Wilcoxon test was also carried out to analyze the physical endurance of mice and it was found that there was a significant difference in the number of mice falls (p=0.000). The results showed that there was a significant increase physical endurance between before and after the treatment in malnutrition induced rats. The P3 group had the most effective dose in improving rat performance.

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