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Contact Name
Arif Rahman Hikam
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Fakultas Biologi Universitas Jenderal Soedirman Jl. dr. Soeparno No. 63 Purwokerto, Kabupaten Banyumas Kode Pos 53122
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Jawa tengah
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BioEksakta : Jurnal Ilmiah Biologi Unsoed
ISSN : -     EISSN : 27148564     DOI : -
Jurnal BioEksakta menerbitkan artikel ilmiah hasil penelitian dalam bidang biologi umum termasuk Ekologi dan konservasi, Taksonomi dan Struktur, Biogeografi, Evolusi, Biodeversitas, Fisiologi dan Reproduksi, Biologi sel, Biologi Molekuler dan Genetika.
Articles 401 Documents
INOKULASI RIZOBAKTERIA PADA TANAMAN CABAI (Capsicum annum L.) DI TANAH SALIN anti, Monica Widi; Oedjijono, Oedjijono; Proklamasiningsih, Elly
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.2140

Abstract

A red chili plant (Capsicum spp.) is one of the horticultural commodities in Indonesia which has good economic value, so it has priority to be developed. Agriculture practices in Java island have partly switched to coastal areas which have characteristics of saline soils. Saline soils are alkaline and nutrient-poor, especially essential nutrients such as P and N. One way to restore fertility in saline soils used beneficial and fertilizing bacteria such as PGPR. Plant growth-promoting rhizobacteria (PGPR) are free-living, soil-borne bacteria, which are capable of enhancing the growth of the plant either directly or indirectly. The objectives of this research were to determain the effect of PGPR isolates to the growth of red chili plants in saline sandy soil and to determine the best PGPR isolate capable to improve the growth of red chili plant in saline sandy soil. The result showed that the inoculation of rhizobacteria significantly affected to plant height and root length of the red chili plant (Capsicum annum). The combination of 3 PGPR isolates (Azospirillum PSA 10, Azotobacter PSA 8, and Marinococcus PSA 1) was the best result in increasing the height and root length of the chili plant (Capsicum annum).
Isolasi dan Uji Resistensi Bakteri Endofit Eceng Gondok Terhadap Krom Nurmalasari, Ade; Oedjijono, Oedjijono; Lestari, Sri
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.2142

Abstract

Endophytic bacteria are bacteria that live in plant tissues and are not pathogenic to the host. One of endophytic bacterial host is water hyacinth. Water hyacinth could accumulate heavy metals, one of which is chrome. The purpose of this study was to obtain endophytic bacteria of water hyacinth (Eichhornia crassipes Mart.), to identify the endophytic bacteria of water hyacinth, and to determine the resistance of endophytic bacteria of water hyacinth to Chrome (Cr). The research method used was a survey with a purposive random sampling technique. Stages of research include root sampling, sterilization of root samples using 2% NaOCl3, isolation of water hyacinth endophytic bacteria on agar nutrient medium (NA), characterization of bacterial endophytes and resistance test of water hyacinth bacteria to chromium. Based on the results of the study, 8 isolates of endophytic bacteria were isolated from the roots of water hyacinth. Based on Bergey's Manual Determinative of Bacteriology, 6 bacterial isolates (E1, E2, E3, E5, E6, E8) were identified as Bacillus and 2 isolates (E4, E7) were identified as Pseudomonas. All water hyacinth endophytic bacteria obtained were resistant to chromium to a concentration of 750 mg/L.
Cultivar Diversity of Balsam (Impatiens balsamina L.) in Banyumas Regency Aulia, Rizki; Widodo, Pudji; Herawati, Wiwik
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.2143

Abstract

Balsam or garden balsam (Impatiens balsamina L.) is a widely grown flowering plant belonging to the family Balsaminaceae. The most conspicuous part to distinguish the balsam is the difference in the flower shape and colors of each cultivar. The purpose of this research is to find out the cultivars diversity of the balsam. The method used in this study was survey with purposive sampling. The variables observed in this study was morphological characteristics including the stem, leaves, flowers, fruits, and seeds. The data obtained were analysed descriptively. The result of this study showed that there were 15 cultivars of I. Balsamina i.e. 'Pinkish White 5 Petal', 'Mix Pink Camellia, 'Pinkish White Camellia, 'Vivid Pink', 'White', 'Light Pink', 'Pinkish White', 'Light Magenta', 'Vivid Red', 'Red Camellia, 'Reddish Camellia, 'Rose Red Camellia, 'Vivid Magenta Camellia, 'Rose Green Camellia, and 'Vivid Pink Camellia’.
PENGARUH SUPLEMENTASI Chlorella vulgaris PADA PAKAN UNTUK MENINGKATKAN ALBUMIN DALAM SERUM IKAN NILEM (Osteochilus vittatus) Zakiah, Kiki Siti; Simanjuntak, Sorta Basar Ida; Wibowo, Eko Setio
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.2144

Abstract

Nilem fish belongs to freshwater fish commodity that has been widely cultivation. Some factors influencing fish cultivation are good quality of feed, quantity, size, and shape. Quality feed works as a primary energy source as well as improves the digestive system of fish, with the expectation that the fish growth and health are optimal. Information concerning health status in cultivation Nilem has been used as a reference for improving cultivation methods and controlling disease attacks. The population and production of fish cultivation is experiencing serious problems due to infection from various types of pathogens. Therefore, a viable alternative is needed to prevent and overcome diseases by utilizing immunostimulant substances derived from synthetic substances that can increase the activity of non-specific immune defenses and stimulate antibiotic-forming organs in fish body. Immunostimulant is a substance that is capable of increasing the mechanism of non-specific immune response of fish one of them is by adding Chlorella vulgaris extract to the feed. This research objective is to determine the influence of Chlorella vulgaris supplementation on total blood albumin in nilem fish. This research was conducted by sampling method consisting of 5 treatments with further 4 replications. The treatments were feed supplementation of 0 g.kg-1, 2 g.kg-1, 3 g.kg-1, 4 g.kg-1, and 6 g.kg-1. Independent and dependent variables were used in this research; the independent variable was the dose of Chlorella vulgaris in the feed and the dependent variable was blood albumin concentration. The measuring of plasma albumin concentration was done using the Dyasis kit method with a spectrophotometer. The obtained data were then analyzed by ANOVA, followed by the Least Significant Difference (LSD) at the 95% confidence level if there is a real difference then followed by Tukey's test at the same confidence level. The results showed that the average value of serum albumin concentration of nilem fish from all treatments was between 4.2761 – 7.1826 g/dL. The normal albumin concentration was found in the treatment 4 g.kg-1 Chlorella vulgaris supplementation which was 4.77 g/dL. Meanwhile, the highest concentration was found from the supplementation of Chlorella vulgaris at 3 g.kg-1 feed which was 6.677 g/dL.
Pengaruh Pemberian Inokulum Fungi Mikoriza Arbuskula (FMA) Campuran terhadap Kemunculan Penyakit Layu Fusarium pada Tanaman Mentimun (Cucumis sativus L.) dan Melon (Cucumis melo L.) Siti Sa'adah, Nadya Sofia; Dwiputranto, Uki; Mumpuni, Aris
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 4 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.2661

Abstract

Mentimun (Cucumis sativus L.) dan Melon (Cucumis melo L.) merupakan contoh dari sayuran dan buah yang banyak di konsumsi oleh masyarakat, serta juga memiliki banyak manfaat. Permintaan pasar untuk mentimun dan melon sangat tinggi, maka produksi harus mengikuti tingginya permintaan pasar. Ada beberapa permasalahan dalam produksi, salah satunya adalah penyakit layu yang disebabkan oleh Fusarium. Secara konvensional, penggunaan fungisida sintetik dianggap sebagai solusi yang tepat untuk mengendalikan penyakit layu fusarium. Namun demikian, dengan mempertimbangkan dampak negatif dari fungisida tersebut, penggunaan Fungi Mikoriza Arbuskula (FMA) menjadi alternatif pilihan yang dapat diaplikasikan. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan dosis berbeda dari inokulum FMA campuran (0, 5, 10, 15, 20 g FMA dengan medium pembawa zeolit / tanaman). Parameter utama adalah intensitas penyakit, sedangkan parameter pendukungnya adalah pH tanah, temperatur, kelembapan, masa inkubasi penyakit, dan derajat infeksi FMA terhadap akar tanaman. Data yang diperoleh dianalisis dengan menggunakan Uji ragam (uji F) denganStandar Kesalahan 5%. Berdasarkan hasil penelitian, tanaman yang diinokulasi dengan inokulum mikoriza lebih tahan terhadap penyakit layu fusarium. Dosis optimal FMA campuran untuk mengurangi intensitas penyakit layu fusarium pada tanaman mentimun adalah adalah M3BT (inokulasi FMA campuran 15 g/tanaman) dan melon adalah M2BM (inokulasi FMA campuran 10 g/tanaman)
Kajian Etnozoologi Biawak (Varanus salvator) dalam Bidang Kesehatan oleh Masyarakat Suku Anak Dalam (SAD) Astuti, Herawati Dwi; Mukharomah, Laelatul; Shobaiyah, Lailatus; Novita, Novita
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.2797

Abstract

Lizard or known as the Asian water lizard (Varanus salvator) is one of the reptiles found in Asia, one of which is in Indonesia. In addition to being used as food, some communities use monitor lizards to treat diseases, especially those who still maintain ancestral customs and beliefs. This study aims to describe the knowledge of local indigenous peoples in utilizing monitor lizards for the treatment of diseases and integrating them with traditional conservation efforts undertaken by the community. The research uses the ethnozoology approach through the literature review method. The results showed that the local people especially Suku Anak Dalam (SAD) in Jambi used monitor lizards for the treatment of skin diseases, hypo-allergies and ringworm by burning or cooking them using cooking oil until the fat in the monitor lizards melted for ointment. However, the local people still try to maintain the balance of monitor lizards by choosing large monitor lizards that are suitable for consumption, using simple hunting tools, and domestication efforts so that the monitor lizard population is maintained in a sustainable manner. This is known because the mechanism of hypo-allergenic treatment of monitor lizard meat has the potential to reduce the effects of allergies given by allergy in the form of histamine 1.5% by 10 ml. In conclusion, the use of monitor lizards in the field of health by the local community wisely can improve conservation skills through traditional ecological knowledge (Traditional Ecological Knowledge)
Keanekaragaman Vegetasi Tumbuhan bawah pada tegakan Jati (Tectona grandis Linn.) di RPH Ciporos Widiyanto, Ody Febri; Wibowo, Dwi Nugroho; Hidayah, Hexa Apriliana
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.2926

Abstract

Abstract: The undergrowth can be used to describe soil conditions that can be seen clearly in the field, because it can increase soil stability, soil fertility and increase land productivity. The purpose of this study was to determine diversity of undergrowth vegetation in teak stands (Tectona grandis Linn.) and environmental factors related to the diversity of undergrowth on teak stands at Ciporos RPH, BKPH Sidareja, KPH Banyumas Barat, Central Java. The research method used was a survey method by sampling using line transects. Data on vegetation diversity of undergrowth were analyzed using Shannon-Wiener diversity index (H ') while the vegetation evenness data was analyzed using Evenness Index (E'). To determine the relationship between environmental factors and undergrowth use Paleontological Statistics (PAST) software analysis. The results showed that the lower ground species found in the Ciporos RPH consisted of 8 species from 7 families. The undergrowth species that has the highest Importance Value Index (INP) is Paspalidium flavidum (Paspalum grass) of the Gramineae family (Poaceae) with 69.21% INP and the lowest is Arum maculatum (Arum flower) of the Araceae family with an INP of 1.38 %. The diversity of undergrowth vegetation in teak stands in RPH Ciporos has a Diversity Index (H ') including moderate and the pattern of distribution of undergrowth species is classified as low. The most environmental factor related to the diversity of u undergrowth vegetation is soil pH. Key Words: diversity; RPH Ciporos; undergrowth Abstrak: Tumbuhan bawah dapat digunakan untuk menggambarkan keadaan tanah yang dapat dilihat secara nyata di lapangan, karena dapat meningkatkan kestabilan tanah, kesuburan tanah dan meningkatkan produktivitas lahan. Tujuan penelitian ini yaitu untuk mengetahui keanekaragaman vegetasi tumbuhan bawah pada tegakan jati (Tectona grandis Linn.) dan faktor lingkungan yang berkaitan dengan keanekaragaman tumbuhan bawah pada tegakan jati pada RPH Ciporos, BKPH Sidareja, KPH Banyumas Barat Jawa Tengah. Metode penelitian yang digunakan adalah metode survey dengan cara pengambilan sampel menggunakan line transect. Data keanekaragaman vegetasi tumbuhan bawah dianalisis menggunakan Indeks keanekaragaman Shannon-Wiener (H’) sedangkan data kemerataan tumbuhan bawah dianalisis menggunakan Indeks Kemerataan (E’). Untuk mengetahui hubungan antara faktor lingkungan dan tumbuhan bawah digunakan analisis Software Paleontological Statistics (PAST). Hasil penelitian menunjukan bahwa spesies tumbahan bawah yang ditemukan di RPH Ciporos terdiri atas 8 spesies dari 7 famili. Spesies tumbuhan bawah yang memiliki Indeks Nilai Penting (INP) tertinggi yaitu Paspalidium flavidum (Rumput Paspalum) dari familia Gramineae (Poaceae) dengan INP 69,21% dan yang terendah yaitu Arum maculatum (Bunga Arum) dari familia Areceae dengan INP Sebanyak 1,38%. Keanekaragaman vegetasi tumbuhan bawah pada tegakan jati di RPH Ciporos memiliki Indeks Keanekaragaman (H’) termasuk sedang dan pola penyebaran spesies tumbuhan bawah tergolong rendah. Faktor lingkungan paling berkaitan dengan keanekaragaman vegetasi tumbuhan bawah yaitu pH tanah. Kata kunci: keanekaragaman vegetasi; RPH Ciporos; tumbuhan bawah
KEANEKARAGAMAN DAN DOMINANSI RUMPUT LAUT HIDROKOLOID PADA SUBSTRAT YANG BERBEDA DI PERAIRAN PANTAI KARANGTENGAHNUSAKAMBANGAN CILACAP Ardiyanto, Bayu; Insan, Achmad Ilalqisny; Widyartini, Dwi Sunu
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.2999

Abstract

The waters of Karangtengah Nusakambangan Cilacap have various types of substrates, namely sand, coral and mixture. Beach conditions with many types of substrates allow various species of seaweed to grow. Information regarding the diversity and dominance of seaweed producing hydrocolloid species has not been much studied in the waters of Karangtengah Beach. This study aims to determine the diversity and dominance of seaweed producing hydrocolloids on different substrates in the waters of Karangtengah Nusakambangan Cilacap. This study uses a survey method with a sampling technique that is the selected random transect method. Six transect lines were made perpendicular to the coastline and the distance between transects was 25 m. Each transect was made three plots, each plot measuring 1x1 m based on the substrate (coral, sand, and mixture). The main parameters observed were the amount of biomass and the number of species. Supporting parameters observed were environmental factors namely temperature, salinity, pH, current speed and depth. The number of hydrocolloid seaweed species found was 10 species of seaweed with details of 5 alginate-producing species, 3 agar-producing species, and 2 carrageenan-producing species. The diversity index value in the coastal waters of Karangtengah between 0,6-2,0 was classified as moderate diversity. The dominance index value in the coastal waters of Karangtengah between 0,2-0,6 was classified as low dominance.
Isolation and Characterization Of Arbuscular Mychorhiza Fungi from Gaharu Wood (Aquilaria spp.) Rhizosphere Wijayanti, Dian Rachma; Turjaman, Maman
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3090

Abstract

In the last 30 years there have been increasing demand of agarwood. Unlimited hunting of agarwood causing it become threatened species. Aquilaria spp. is the main genus producing agarwood that considered as threathened species. Conserving Aquilaria spp. as germ plasm needs is essential against destruction. Arbuscular mycorrhizal fungi (AMF) have been used as inoculant to increase the growth of Aquilaria spp., but information about the existence of AMF in Aquilaria spp. rhizosphere has not been reported. This research was carried out with objective to identify AMF from Aquilaria spp. rhizosphere. Soil and root samples from rhizosphere of Aquilaria spp. taken from four location that is: Carita (A. malaccensis), Ciapus (A. filaria), Cifor (A. crasna & A. malaccensis), and Bantar Kemang (A. beccariana, A. crasna, & A. malaccensis). Roots staining was conducted with modified Phillips and Hayman procedure (1970). Soils sample was run dry wed and placed in pot culture with Pueraria javanica as the host plant. Spore was separated from pot culture using wet sieving and decanting method continued with centrifugation. The result showed the occurrences of AMF in Aquilaria spp. roots i.e. external hypha, internal hypha, coiled hypha, arbuscules, and vesicles. All AMF colony structure found in Aquilaria spp. roots show asosiation bettwen the plant and AMF. Four species of AMF were found i.e. Glomus sp.1, Glomus sp.2, Gigaspora albida, and Dentiscutata sp. Key Words: Isolation, Arbuscule Mycorrhizae Fungi, Rhizosfer, Agarwood
Pengaruh Jenis dan Konsentrasi Bahan Tambahan Terhadap Pertumbuhan Miselium Jamur Paha Ayam (Coprinuscomatus) Saputra, Wahyu Dwi; Ratnaningtyas, Nuniek Ina; Mumpuni, Aris
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.3091

Abstract

Abstrak Coprinus comatus merupakan salah satu jamur pangan yang potensial untuk dibudidayakan secara komersial. Jamur C. comatus termasuk ke dalam jamur edible dan medicinal. Tubuh buah C. comatus mengandung antioksidan dan beberapa asam amino esensial. Jamur C. comatus tumbuh optimal pada medium yang mengandung suplemen. penambahan suplemen pada medium tanam jamur bertujuan untuk memberikan nutrisi yang dibutuhkan jamur. Suplemen ditambahkan dengan jumlah tertentu. Penelitian ini bertujuan untuk mengetahui kombinasi jenis suplemen dan konsentrasi suplemen yang optimal untuk pertumbuhan jamur C. comatus. Penelitian dilakukan dengan metode eksperimental menggunakan Rancangan Acak Lengkap pola Faktorial dengan dua faktor uji yaitu jenis dan konsentrasi suplemen. Suplemen yangdigunakan yaitu dedak padi (bekatul), dedak jagung (ampok), dedak gandum (polar), dan tepung gandum. Konsentrasi suplemen yang digunakan yaitu 0%, 5%, 10%, 15%, dan 20%. Hasil yang didapat dari penelitian ini yaitu penambahan suplemen dengan Konsentrasi berbeda berpengaruh nyata terhadap pertumbuhan miselium jamur C. comatus. Suplemen yangbaik untuk menumbuhkan jamur C. comatus adalah ampok jagung dan dedak padi. Konsentrasi yang optimal untuk pertumbuhan miselium jamur C. comatus adalah dedak jagung dengan konsentrasi 5% dan dedak padidengan konsentrasi 15%. KataKunci : Coprinus comatus, kecepatan pertumbuhan miselium, penambahan suplemen