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Bioscientist : Jurnal Ilmiah Biologi
Published by Universitas Pendidikan Mandalika
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,439 Documents
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Analisis Tingkat Kognitif Pada Soal Buku Teks IPA Kelas VII Berdasarkan Taksonomi Bloom (Revisi) Sholihah, Afifah Maratush; Astuti, Dwi Setyo
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14911

Abstract

This study investigated the distribution of cognitive levels within the questions of the Grade VII science textbooks published by the Ministry of Education and Culture in 2021, using the revised Bloom’s taxonomy as an analytical framework. The revised Bloom’s taxonomy categorizes cognitive processes into six distinct levels: C1 (remembering), C2 (understanding), C3 (applying), C4 (analyzing), C5 (evaluating), and C6 (creating). This study adopted a descriptive research design with a qualitative approach. This study utilizes documentation analysis as its primary data collection method, involving thorough reading and systematic evaluation of the textbook content. The findings indicate the following distribution of cognitive levels across the questions: C1 (Remembering) accounted for 14 questions (15.7%), C2 (Understanding) for 28 questions (31.5%), C3 (Applying) for 15 questions (16.9%), C4 (Analyzing) for 13 questions (14.6%), C5 (Evaluating) for 1 question (1.1%), and C6 (Creating) for 18 questions (20.2%). These results reveal a lack of diversity and proportionality in the distribution of cognitive levels, underscoring an imbalance in the representation of higher-order thinking skills.
Project Based Learning: Using Digital Storytelling to Improve Generation Z Students’ Botanical Literacy in Botanical Course Anggarani, Dinar Arsy; Sari, Murni Sapta; Sulisetijono, Sulisetijono
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14916

Abstract

This study aims to examine the effectiveness of implementing PjBL combined with DS in enhancing botanical literacy among biology students. This research follows a quasi-experimental non-equivalent control group design. The botanical literacy scores of students are normally distributed, and the data variance is homogeneous (sig>0.05). The findings indicate that the implementation of Project-Based Learning (PjBL) supported by digital storytelling can improve students' botanical literacy. The results of the LSD (Least Significant Difference) post-hoc test show no significant difference between the experimental group using PjBL with digital storytelling and the control group using PjBL without digital storytelling. There is an indication that the use of artificial intelligence (AI) in answering post-test questions may have influenced the research results. Nonetheless, digital storytelling remains a promising innovative approach in botany education. Its advantages during the learning process are evident in students' higher engagement, increased creativity in presenting material, and its ability to visualize complex botanical concepts effectively. Further integration of other technologies in learning is necessary, along with stricter control over AI usage during assessments.
Pengaruh Konsentrasi Air Cucian Beras terhadap Pertumbuhan Anggrek Dendrobium sp. Pasca Aklimatisasi Hartanto, Maisabita Farah Amila; Dewi, Endah Rita Sulistya; Rahayu, Praptining
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14938

Abstract

This study aims to investigate how various concentrations of rice-washed water influence the growth of Dendrobium sp. in the post-acclimatization phase. A Completely Randomized Design (CRD) with one factor and four treatments was used: P0 (0% rice-washed water), P1 (30% rice-washed water), P2 (40% rice-washed water), and P3 (50% rice-washed water). The statistical analysis was conducted using a One-Way ANOVA at a 5% level. If significant differences were found, further analysis was performed using Duncan's Multiple Range Test. The results of data analysis revealed that (1) There were no significant differences in plant height and root length of Dendrobium orchids across the treatments during the post-acclimatization phase; (2) Treatments P1 to P3 did not yield optimal results in terms of plant height, as these concentrations were not suitable for supporting the height growth of Dendrobium orchids in the post-acclimatization phase; (3) The P2 concentration resulted in the longest root growth at 2.39 cm, while the shortest root length was observed in the P0 concentration. Thus, although the different concentrations of rice washing water did not produce statistically significant differences, the P2 concentration showed potential for promoting root growth in Dendrobium orchids during the post-acclimatization phase.
Pengembangan Aplikasi Mobile Plant-tagging ‘Pelurutaloka’ sebagai Media Pembelajaran Biologi Keanekaragaman Hayati Hidayat, Muhammad Luthfi; Mahatama, Duta Saksena; Suryani, Titik; Sari, Siti Kartika
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.14948

Abstract

This research aims to develop a web-based mobile plant-tagging application that connects local plant species to scientific information through QR codes, which emphasizes contextual, technology-assisted learning grounded in local potential. The resulting educational media, titled Pelurutaloka (an acronym for Pembelajaran Luar Ruang Tanaman Lokal, or Outdoor Learning of Local Plants), is specifically designed to be pedagogically relevant and to serve as a foundation for future studies in local natural resources.This study adopts a Research and Development (R&D) methodology using the 4D model (Define, Design, Develop, Disseminate), with development limited to the evaluation/revision stage. The evaluation phase employed a 14-item Likert-scale questionnaire, which demonstrated high levels of validity and reliability based on expert review and Rasch model analysis (reliability = 0.72). Further Rasch analysis revealed a unidimensionality value of 40.2%, exceeding the acceptable threshold of 20%, indicating that the instrument effectively measured a single construct. The primary contribution of this research lies in the creation of a web-based, mobile-accessible biology learning media designed for outdoor contexts, offering an engaging, technology-enhanced learning experience that promotes student activity and interaction with the environment. This study recommends broader dissemination of the Pelurutaloka platform and its implementation in outdoor biology education, particularly through collaborative, active learning models such as problem-based learning (PBL) and project-based learning (PjBL).
Evaluasi Aktivitas Antibakteri Minyak Jintan Hitam Terhadap Bakteri Gram-Positif dan Gram-Negatif: Studi pada Staphylococcus aureus dan Pseudomunas aeruginosa Salsabila, Maisan Khoiri; Rukaya, Benazir Evita; Syuhada, Syuhada
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14950

Abstract

This study aims to evaluate the antibacterial activity of black cumin oil (Nigella sativa) against Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) bacteria. Methods: The antibacterial activity was tested using the well diffusion method with varying concentrations of black cumin oil (0.1%, 0.5%, 1%, and 2%), along with a negative control using ethyl acetate as the solvent. Additionally, a simple phytochemical screening was conducted using a test tube reaction method to detect the presence of active compounds such as alkaloids, flavonoids, tannins, and saponins. The results showed that (1) black cumin oil at a concentration of 2% produced the largest inhibition zones, measuring 15.33 ± 3.21 mm for Staphylococcus aureus and 9.37 ± 1.98 mm for Pseudomonas aeruginosa; (2) Statistical analysis confirmed significant differences in several treatments, with a p-value < 0.05. The antibacterial effect of black cumin oil was stronger against Gram-positive bacteria than Gram-negative bacteria, likely due to differences in bacterial cell wall structures; (3) Furthermore, black cumin oil tested positive for alkaloids and saponins, which are known as potential antimicrobial compounds. Overall, this study indicates that black cumin oil has the potential to serve as an effective natural antibacterial agent, particularly against Gram-positive Staphylococcus aureus.
Analisis Filogenetik Cryptic species Apis cerana Fabricius, 1793 Berdasarkan Gen 16S rRNA Puspitasari, Amira; Budiarsa, I Made; Ashari, Abdul; Dhafir, Fatmah; Windarsih, Yulia; Trianto, Manap
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14967

Abstract

Apis cerana is a member of the Insecta class grouped in the order Hymenoptera. Phylogenetic analysis is used to describe the kinship of living things through phylogeny trees. This study aims to describe the phylogenetic relationship of cryptic species in Apis cerana based on the 16S rRNA gene. Sampling use the cruising method in various regions, the DNA isolation, DNA amplification, sequencing, followed by bioinformatics analysis using GeneStudio, DNASTAR, MESQUITE, and MEGA 11. The result showed that phylogenetic analysis with the Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods produced a phylogenetic tree that illustrates clear evolutionary relationship. A. cerana from Central Sulawesi is more closely related to other A. cerana species in the same clade, while other species are separated. The result of this study show a clear pattern of kinship between A. cerana populations.
Analisis Filogenetik Lebah Madu Endemik Sulawesi Apis nigrocincta Smith, 1860 Berdasarkan Gen 16S rRNA Hidyanisa, Dhea; Budiarsa, I Made; Ashari, Abdul; Trianto, Manap; Nurdin, Musdalifah; Windarsih, Yulia
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14968

Abstract

Apis nigrocincta is an endemic honey bee that is only found in two regions in Indonesia, namely the Sangihe Islands and Sulawesi Islands. Phylogenetic analysis is used to describe the phylogenetic relationship of Sulawesi endemic honey bee A. nigrocincta based on the 16S rRNA gene. Sampling using the cruising method in various regions, then DNA isolation, DNA amplification, sequencing, and continued bioinformatics analysis using GeneStudio, DNASTAR, MESQUITE, and MEGA 11. The results showed that phylogenetic analysis with the Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods produced a phylogenetic tree that illustrates clear evolutionary relationships. A. nigrocincta from Central Sulawesi is more closely related to other A. nigrocincta species in the same clade, while other species are separated. The results of this study show a clear pattern of kinship between A. nigrocincta populations. 
Analisis Filogenetik Lebah Tukang Kayu Xylocopa appendiculata Smith, 1852 Berdasarkan Gen Cytochrome Oxidase 1 (CO1) Rahayu, Irma Septi; Budiarsa, I Made; Ashari, Abdul; Trianto, Manap; Masrianih, Masrianih; Rafiqa, Rafiqa
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14969

Abstract

Xylocopa appendiculata  is an insect from the order Hymenotera and has bright yellow morphological characteristics on the thorax, while the rest of the body is black. Phylogenetic analysis is used to describe the kinship of living things throughh phylogeny trees. This study aims to describe the phylogenetic relationship of carpenter bees Xylocopa appendiculata based on the cytochrome oxidase 1 (CO1). DNA isolation, DNA amplification, sequencing, followed by bioinformatics analysis using GeneStudio, DNASTAR, MESQUITE, and MEGA 11. The results showed that phylogenetic analysis using Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods produced a phylogenetic tree that illustrates a clear evalutionary relationship X. appendiculata from Central Sulawesi is more closely related to other X. appendiculata species in the same clade. The results of this study show a clear kinship pattern between populations of X. Appendiculata.
Karakterisasi Morfologi dan Biokimiawi Berbagai Kultivar dan Aksesi Jeruk Pamelo (Citrus maxima (burm.) merr.) di Kabupaten Magetan Istiqomah, Intan Lailatul; Suratman, Suratman; Solichatun, Solichatun
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14984

Abstract

This study examines the morphological characteristics, sugar content, and antioxidant activity of various cultivars and accessions of Pomelo Orange in Magetan Regency. Samples were taken using a purposive sampling method. Morphological characters were observed based on IPGRI characterization, while antioxidant activity analysis was carried out using a UV-Vis spectrophotometer. Morphological character data were analyzed descriptively and antioxidant activity was analyzed by One Way Anova method and followed by DMRT (Duncan's Multiple Range Test) test. The results showed that (1) the highest inhibition percentage was found in the Srinyonya cultivar in Takeran, which was 76.15%; (2) Overall, Pomelo Orange cultivars have antioxidant activity with IC50 values ranging from 5.78–27.17 ppm, indicating strong antioxidant activity; (3) The sugar content in various cultivars ranges from 8–10 °Brix, with the highest levels found in the Javanese cultivar in Sukomoro, which was 10.7 °Brix.
Efektivitas Salep Minyak Jintan Hitam (Nigella sativa) sebagai Antibakteri terhadap Staphylococcus aureus dan Pseudomonas aeruginosa Arifullah, Muhamad Aqsal; Rukaya, Benazir Evita; Syuhada, Syuhada
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.15005

Abstract

This study aimed to evaluate the effectiveness of black cumin oil ointment as an antibacterial agent against Staphylococcus aureus and Pseudomonas aeruginosa. The ointment was formulated with three variations of black cumin oil concentrations (0.1%, 1%, and 10%) using a lipophilic base. The physical stability test was conducted by evaluating organoleptic properties, homogeneity, pH, spreadability, and adhesion. The antibacterial activity was tested using the well-diffusion method against Staphylococcus aureus and Pseudomonas aeruginosa and analyzed using the One Way ANOVA method followed by a post hoc Tukey test. The results of this study indicate that (1) The black cumin oil ointment demonstrated good physical stability, particularly in terms of homogeneity and pH; (2) The antibacterial activity test showed that the ointment with a 10% black cumin oil concentration (F3) effectively inhibited the growth of Staphylococcus aureus with an efficacy not significantly different from gentamicin ointment (p = 0.477), but significantly different from the negative control (p = 0.027). However, no antibacterial activity was observed against Pseudomonas aeruginosa.Conclusion: The 10% black cumin oil ointment formulation has the potential as a natural antibacterial agent against Staphylococcus aureus. These findings suggest that black cumin oil can be further developed as a natural-based topical therapy alternative for treating skin infections caused by Gram-positive bacteria.

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