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INDONESIA
Bioscientist : Jurnal Ilmiah Biologi
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Articles 1,439 Documents
Eksplorasi Peran Reactive Oxygen Species (ROS) yang diinduksi oleh Ekstrak Caesalpinia Sappan untuk Air yang Terkontaminasi Anabaena sp. Mahendra, Himawan Akbar; Supraba, Intan; Kamulyan, Budi; Suyono, Eko Agus
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14756

Abstract

This study aims toexplore the reduction of Anabaena sp. populations using Caesalpinia sappan extract, aiming to determine the optimal concentration to induce oxidative stress that weakens cell structures via increased production of Reactive Oxygen Species (ROS). Water quality parameterssuch as color, turbidity, and Total Dissolved Solids (TDS)were analyzed to evaluate the extract's effectiveness on the aquatic ecosystem. Employing a combination of the TRIZ (Theory of Inventive Problem Solving) method and a True Experimental design, the study optimized extract concentration variations and examined the ability of Caesalpinia sappan (sappan wood) to penetrate the peptidoglycan layer of Anabaena sp. Results obtained over two days indicate that the bioactive compounds in Caesalpinia sappan induce ROS, as evidenced by an increase in color intensity from day 1 to day 2 due to pigment release from Anabaena sp. in response to oxidative stress. Higher extract concentrations led to a significant reduction in Anabaena sp. populations, with the 100% concentration yielding an 86% decrease on day 2, alongside a color reading of 1209 Cu, turbidity of 39.6 NTU, and TDS of 114.5 mg/l. Microscopic observations revealed notable cell disintegration and damage in Anabaena sp. This study confirms that bioactive compounds, such as brazilin and flavonoids in Caesalpinia sappan, contribute to ROS generation, leading to cell death in Anabaena sp. via oxidative stress.
Exploring the Level of AI (Artificial Intelligence) Digital Literacy and Creative Thinking Skills in High School Students Zebua, Nofamataro; Ibrohim, Ibrohim; Sulisetijono, Sulisetijono
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14782

Abstract

The development of Artificial Intelligence (AI) in education has opened up opportunities for strengthening AI digital literacy and creative thinking skills among students. AI digital literacy includes understanding how AI works, the ethics of use, and utilization in various contexts, while creative thinking refers to the ability to generate innovative and original ideas. This study aims to explore the level of AI digital literacy and creative thinking skills among Xth grade high school students, using a descriptive method with proficiency tests and self-assessment questionnaires. The results showed that students' AI digital literacy level was low, with an average score of 56.94%, where students had difficulty in critically digesting information, recognizing the social impact of AI, using AI technology, and considering the ethical aspects of its use. In addition, students' creative thinking skills were also low, with an average score of 55.14%, especially in the aspects of originality of ideas, flexibility of thinking, and conceptual mapping. These findings highlight the need for the integration of an AI curriculum in education, the use of interactive AI tools, and the implementation of project-based learning models to improve students' AI digital literacy and creativity. In addition, learning environments that support exploration and positive feedback from teachers play an important role in creating optimal synergy between these two skills. Further research is needed to explore the limitations of AI in supporting creativity as well as the risks of technology dependency in the innovative thinking process.
Potensi Metabolit Sekunder Tanaman Syzygium sp. Terhadap LOX-1 Sebagai Target Terapi Penyakit Aterosklerosis: Studi In Silico Raizma, Elda Zaelita Nurul; Sopian, Devia Angelina; Alifianto, Handika Maulana; Eshananda, Yuriza; Simanjuntak, Sorta Basar Ida
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14817

Abstract

Atherosclerosis is a cardiovascular disease characterized by the accumulation of oxidized lipoproteins in the arterial wall, which is triggered by LOX-1 receptor activation. The members of Syzygium genus contains secondary metabolites with antioxidant and anti-inflammatory activities that have the potential to inhibit LOX-1. This study aims to evaluate the interaction of compounds from Syzygium cumini, Syzygium aromaticum, Syzygium malaccense, and Syzygium jambos against LOX-1 using in silico method. Analysis was conducted through molecular docking, pharmacophore selection based on hydrophobic and hydrogen bond interaction criteria, and validation using Root Mean Square Deviation (RMSD ≤ 3 Å) values. The research results showed that gallic acid has the highest affinity (-4.1 kcal/mol), followed by linalool (-3.8 kcal/mol), kaempferol (-3.4 kcal/mol), oxalic acid (-3.4 kcal/mol), quercetin (-2.2 kcal/mol), and myricetin (-1.4 kcal/mol). The compounds with the highest affinity interacted with key LOX-1 residues, suggesting potential inhibition of the receptor. In conclusion, gallic acid is the most potent LOX-1 inhibitor candidate based on its stable interaction and highest affinity value. Further studies are needed to confirm its biological activity through in vitro and in vivo approaches.
Komunitas Belajar dalam Lesson study untuk Memberdayakan Kognitif dan Psikomotor Siswa pada Pembelajaran Biologi di Luar Kelas Zukmadini, Alif Yanuar; Lestari, Puji; Rochman, Syaiful; Karyadi, Bhakti
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.14844

Abstract

This study aims to enhance cognitive and psychomotor abilities through outdoor learning, designed and evaluated collaboratively by the learning community within the framework of the Lesson study. The study was conducted on grade X students of MAN Insan Cendekia Bengkulu Tengah. Lesson study was carried out in two cycles, each consisting of the plan, do, and see stages. The "plan" stage is the planning stage to prepare the learning tools and strategies that will be carried out. The "do" stage is the implementation of the learning that has been designed. The "see" stage is a reflection of the learning activities that have been carried out. The instruments used consist of observation sheets and test paper. Data is analyzed by calculating the average percentage of each observation indicator, while cognitive and psychomotor learning outcomes are analyzed by calculating the average value obtained. Implementation of the lesson study achieved a percentage score of 100%. Cognitive learning outcomes from cycle I to cycle II increased by 20.43%. Meanwhile, psychomotor learning outcomes increased by 26.79%. The ecosystem acts as a direct learning context that does not only present objects of study to be studied but can also facilitate students to develop conceptual understanding and scientific skills simultaneously. Thus, the reflection carried out will encourage teachers to make continuous improvements. The conclusion of this study is that lesson study activities involving the role of the learning community can improve the planning, implementation, and reflection of biology learning outside the classroom, thus having a significant impact on improving students' cognitive and psychomotor learning outcomes.
Hubungan Profil Lipid dan Kejadian Migren pada Karyawan di Jakarta Satyasari, Daniella; Larasari, Andira; Faradilla, Meutia Atika; Anastasya, Karina Shasri
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14885

Abstract

One of the risk factor for migraines is an elevated lipid profile. The most significant of the primary lipoproteins that causes atherosclerosis and readily binds to blood vessel walls is LDL. Because lipoprotein A (Lp(a)) levels have an atherogenic ApoA structure, increasing oxidative stress and fat oxidation can lead to endogenous vascular injury. Cross-sectional design was used to perform this study from October 2023 to June 2024 in Universitas Trisakti, Faculty of Medicine. The purpose of this study is to evaluate the association between the lipid level and the incidence of migraines among employees in Jakarta. The target population consists of Jakarta's private employees. With a total sample size of 66 employees that meet the inclusion and exclusion criteria, the study sample will be drawn using the sequential sampling method. The study's findings highlighted that high LDL (p=0.002) and total cholesterol (p=0.0001) had a significant relationship with migraines, whereas HDL, triglycerides, and Lp(a) had an insignificant link with the incidence of migraine (p>0.05). The study concluded that LDL is a powerful predictor of migraine occurrence and has a significant association with migraine.
Korelasi Keterlaksanaan Praktikum dengan Keterampilan Berpikir Kritis pada Siswa SMA di Kota Pontianak Aldiansyah, Rifki; Candramila, Wolly; Murdiyaningsih, Asriah Nurdini
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14889

Abstract

This study aims to describe the implementation of laboratory practices, assess students' critical thinking skills, and analyze the relationship between these aspects among senior high school students in Pontianak City who have adopted the Merdeka Curriculum. This research employs a survey method with a descriptive quantitative approach. The research instruments consist of a questionnaire and a test based on the five critical thinking indicators proposed by Ennis. Data were analyzed through normality, homogeneity, and heteroscedasticity tests, followed by Pearson correlation analysis to examine the relationship between variables. The results indicate that (1) the implementation of laboratory practices and (2) students' critical thinking skills fall into the "good" category, with scores of 70.59% and 73.40%, respectively. (3) Correlation analysis reveals a positive and significant relationship between the two variables, with a correlation coefficient of 0.920 (p = 0.02). Thus, it can be concluded that effective laboratory practices contribute to the enhancement of students' critical thinking skills.
Potensi Tumbuhan Melati Air (Echinodorus radicans) Terhadap Penurunan Kadar Timbal (Pb) dan Tembaga (Cu) Pada Limbah Cair Home Industry Batik di Desa Sendang Kabupaten Lamongan Fatiha, Irssa Intan; Firdhausi, Nirmala Fitria; Zummah, Atiqoh
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14892

Abstract

This study aims to determine the potential of water jasmine plants for optimal absorption time, reduction of heavy metal levels of Pb and Cu in Sendang batik liquid waste, and to observe plant morphology. This study uses an experimental method with a one-factor Completely Randomized Design (CRD) and four repetitions, resulting in 16 experimental plots. Heavy metal testing uses an Atomic Absorption Spectrophotometer (AAS). The results showed that (1) on days 9, 12, 15, and 18 of exposure, the decrease in Pb heavy metal levels was 3.71, 2.65, 0.80, and 0. 14 ppm, heavy metal Cu levels decreased consecutively by 4.09, 2.85, 0.31 and 0.26 ppm. (2) The average absorption rate of heavy metal Pb at exposure times of 9, 12, 15, and 18 consecutive days was 0.8, 0.14, 0.24 and 0.23 ppm, respectively, and the heavy metal Cu was 0.23, 0.28, 0.40 and 0. 33 ppm. (3) Morphological changes, namely the appearance of 2-3 new shoots for each treatment, the stems drooping slightly, the leaves changing color to yellow to brownish and brown spots throughout the treatment and on the 18th day one of the leaves died. (4) The initial results of the statistical test for Pb and Cu metals have a sig value of >0.05, where the data is normally distributed and homogeneous. (5) In the One Way Anova test results, sig = 0.000˂0.05 or there is an effect of exposure time and the post hoc Pb test results are ˂0.05 while some Cu metals are ˃0.05.
Analisis Filogenetik Apis dorsata (Fabricius 1793) Bedasarkan Gen Chytochrome Oxidaxe I (COI) Delima, Nur; Masrianih, Masrianih; Trianto, Manap; Windarsih, Yulia; Buntu, Amalia; Dhafir, Fatmah
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14899

Abstract

Apis dorsata is a giant honeybee species distributed across South and Southeast Asia. Phylogenetic analysis is used to illustrate the evolutionary relationships of organisms through a phylogenetic tree. This study aims to describe the genetic relationships of A. dorsata based on the cytochrome oxidase I (COI) gene. Sampling was conducted using an exploratory method in various regions, followed by DNA isolation, DNA amplification, sequencing, and bioinformatics analysis using GeneStudio, DNASTAR, MESQUITE, and MEGA 11. The results show that phylogenetic analysis using the Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods produced a phylogenetic tree that clearly depicts evolutionary relationships. A. dorsata from Central Sulawesi is more closely related to other A. dorsata species within the same internal cluster, while other species are separated. These findings reveal a clear pattern of genetic relationships among A. dorsata populations.
Pengembangan E-LKPD Berbasis Problem Based Learning (PBL) Terintegrasi Al-Quran dan Hadis Pada Materi Keanekaragaman Tumbuhan Putri, Adinda Opmila; Ulfa, Syarifah Widya
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.14900

Abstract

This study aims to develop a digital student worksheet (E-LKPD) utilizing Liveworksheet, based on Problem-Based Learning (PBL) and integrated with values from the Qur'an and Hadith, focused on the topic of Plant Diversity. The research employed a Research and Development (R&D) approach using the ADDIE model (Analysis, Design, Development, Implementation, Evaluation). Research instruments included expert validation questionnaires for content and media, teacher and student response questionnaires to assess practicality, as well as pre-test and post-test instruments to evaluate effectiveness. The trial subjects were students from classes X MIPA-1 and X MIPA-2 at SMA MAL Medan, involving 60 students and one biology teacher. Validation results showed that the developed E-LKPD was rated as highly relevant by content experts (93.75%) and media experts (97.41%). Practicality assessment yielded scores of 85.7% by the biology teacher and 93.5% by the students, both categorized as very practical. Effectiveness testing showed a learning outcome improvement of 87.4%, indicating a high level of effectiveness. Thus, the PBL-based E-LKPD integrated with Qur'anic and Hadith values on the topic of Plant Diversity is valid, practical, and effective, making it suitable for implementation in high school biology instruction.
Efektivitas Model Pembelajaran Problem Based Learning Terhadap Literasi Sains Siswa Pada Materi Klasifikasi Makhluk Hidup Kelas VII SMP Andayani, Anindya Aulya; Agustina, Lina
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14909

Abstract

This study aims to analyze the effectiveness of the Problem-Based Learning (PBL) model in improving students’ scientific literacy. The research method used is pre-experimental with a one-group pretest-posttest design. The research sample consists of 32 seventh-grade students from class VII F, selected using purposive sampling techniques. Data collection techniques include a scientific literacy test conducted at the beginning and end of the study, supplemented by interviews with biology teachers regarding the implementation of the Problem-Based Learning model. The results of the study indicate a significant improvement in students’ scientific literacy after the implementation of the PBL model. The average pretest score of 64.72 (categorized as Moderate) increased to 83.25 (categorized as Very High) in the posttest. The distribution of scientific literacy improvement shows that 31.25% of students experienced a high increase, 59.38% a moderate increase, and 9.38% a low increase. These findings suggest that PBL enhances students’ understanding of scientific concepts, particularly in problem-solving and scientific data analysis. Thus, it can be concluded that Problem-Based Learning (PBL) is an effective teaching method for improving students’ scientific literacy.