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Laury Marcia Ch. Huwae
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Jurusan Biologi - Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Pattimura Jl. Ir. M. Putuhena, Kampus Poka, Ambon, 97234, Maluku, Indonesia
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Biofaal Journal
Published by Universitas Pattimura
ISSN : -     EISSN : 27234959     DOI : https://doi.org/10.30598/biofaal.v1i1y2020
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Materials Science & Nanotechnology Medicine & Pharmacology
Biofaal Journal merupakan jurnal online yang menerima dan mempublikasikan tulisan-tulisan ilmiah dalam bentuk artikel hasil penelitian terbaru, short communication, maupun review terhadap artikel dari bidang keilmuan Biologi, Faal Tumbuhan, Faal Hewan, Faal Manusia dan Faal Olahraga. Biofaal Journal diterbitkan oleh Profesi Ahli Ilmu Faal, Jurusan Biologi FMIPA Universitas Pattimura. Dalam setahun Biofaal Journal memiliki 1 volume dan 2 Issue. Dewan editor, berasal dari rumpun ilmu biologi dan fisiologi yang aktif dalam publikasi ilmiah skala nasional dan internasional, sehingga mampu melakukan proses review secara profesional. Artikel yang diterima akan diterbitkan sesuai dengan waktu yang ditentukan yaitu pada bulan Juni dan Desember, dan akan tersedia gratis bagi pembaca
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 89 Documents
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HUBUNGAN KEDALAMAN DAN WAKTU PENGAMATAN DENGAN JUMLAH SPAT KERANG HIJAU (Perna viridisi) DIPERAIRAN PANTAI DESA WAIHERU, TELUK AMBON BAGIAN DALAM Rettob, Mujahiddin P R; Eddy, La; Suriani, Sanita
Biofaal Journal Vol 5 No 2 (2024): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v5i2pp074-083

Abstract

The adhesion of green mussel spit is highly correlated with depth. This study aims to establish the correlation between the depth and duration of observation with the abundance of green mussel spat (Perna viridis) in the coastal waters of Waiheru Village, located in Inner Ambon Bay. This study was undertaken utilising three floating net cages (KJA) possessed by local fishermen. The KJA utilised in this investigation had dimensions of 2.0 x 2.5 metres. Each KJA is equipped with 4 threads of hanger rope, resulting in a total of 12 hangers. Each rope hanger is positioned at specific depths, specifically at depths of 3, 6, and 9 metres. The net is 40 cm by 40 cm. Observations were conducted on days 2, 4, 6, 8, 10, 12, and 14 after the nets were strung. The findings indicated that the depth and duration of observation significantly influenced the quantity of green mussel spat. Specifically, more spat was observed compared to other days, suggesting a stronger attachment at that depth and observation period. Nevertheless, on the 10th day of observation, there was a surge in the quantity of green mussel spit.
PENGARUH PENAMBAHAN MADU GALO-GALO TERHADAP AKTIVITAS ANTIBAKTERI KOMBUCHA KULIT NANAS DAN AIR KELAPA Wati, Linda; Novelina, Novelina; Koja, Reni; Sari, Ratni Kumala
Biofaal Journal Vol 5 No 2 (2024): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v5i2pp107-115

Abstract

Kombucha is a fermented beverage known for its various health benefits, one of which is its antibacterial properties, making it a potential base for soap production. This study aims to evaluate the effect of adding Galo-Galo honey on the antibacterial activity of kombucha made from pineapple peel and coconut water waste. Fermentation was carried out for 15 days, and observations were made on pH value, total titratable acidity (TTA), and antibacterial activity against Escherichia coli using the disk diffusion method. The study was designed using a completely randomized design with three repetitions. The results showed that the higher the concentration of Galo-Galo honey added, the more significantly the kombucha pH decreased (p < 0.05), with the lowest pH found in the 25% honey treatment (3.09 ± 0.02). The highest total titratable acidity was also found in the 25% honey treatment (1.33 ± 0.11), along with the decrease in pH, indicating an increase in organic acid content. The antibacterial activity test showed that kombucha with a higher honey concentration had a larger inhibition zone against E. coli, with the largest inhibition zone found in the 25% honey treatment (11 ± 1.26 mm). The addition of Galo-Galo honey was proven to enhance the chemical properties of kombucha and strengthen its antibacterial activity.
STRUKTUR POPULASI DAN POLA DISTRIBUSI PALA (Myristica fragrans Houtt) PADA PERKEBUNAN PALA DI DUSUN MANGKOBATU BANDA NEIRA MALUKU TENGAH Lawansuka, Gielldy; Tuhumuri, Evelin; Sahertian, Dece Elisabeth
Biofaal Journal Vol 5 No 2 (2024): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v5i2pp130-138

Abstract

Nutmeg (Myristica fragrans Houtt) is a native plant of Indonesia and endemic to the Banda Islands, Maluku. Nutmeg plantations are scattered across various locations in Banda Neira, one of which is in Mangkobatu Village. The population structure in nutmeg plantations is essential for predicting the future regeneration of trees to replace the old ones. This study aims to describe the population structure and analyze the distribution pattern of nutmeg in the plantations of Mangkobatu Village, Banda Neira, Central Maluku. The research used the quadrat method to count individual nutmeg trees for analysis of density, sex ratio, and distribution patterns. The results show that the population structure of nutmeg in the plantations of Mangkobatu Village consists of seedling stages with a density of 250 individuals/ha, sapling stages with 380 individuals/ha, pole stages with 190 individuals/ha, and mature trees with 68.75 individuals/ha. The population structure forms a bell-shaped polygon diagram. The sex ratio of nutmeg in the plantations of Mangkobatu Village is 1:27, which does not align with the recommended ratio for nutmeg plantations. The distribution pattern of nutmeg at the seedling stage is clumped (Ip = 1), while at the sapling (Ip = -0.464), pole (Ip = -0.357), and mature tree (Ip = -0.567) stages, the distribution is uniform.
Efficacy Test of Ethanol Extract From Matoa (Pometia pinnata) Stem Bark on Aedes aegypti Larval Mortality As An Effort to Control Dengue Fever Moniharapon, Debby Dijola; Moniharapon, Mechiavel
Biofaal Journal Vol 6 No 1 (2025): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v6i1pp1-9

Abstract

Dengue Hemorrhagic Fever (DHF) is an infectious disease caused by the dengue virus and transmitted primarily through the bite of the Aedes aegypti mosquito, the disease's primary vector. Controlling the population of this mosquito species is considered a strategic measure in preventing the spread of DHF. One potential biological control agent is the matoa plant (Pometia pinnata), a member of the Sapindaceae family widely distributed in tropical regions. This plant contains secondary metabolites such as flavonoids, tannins, and saponins, which exhibit natural insecticidal activity. This study aims to evaluate the efficacy of ethanol extract derived from the bark of P. pinnata against A. aegypti larvae. The extraction process was conducted using ethanol as a solvent, and the resulting extract was tested against mosquito larvae at concentrations of 25 ppm, 50 ppm, 70 ppm, and 90 ppm. Larval mortality data were analysed using Analysis of Variance (ANOVA) via SPSS software version 24.00. The results demonstrated that the ethanol extract of P. pinnata bark had a significant dose-dependent effect on larval mortality. These findings suggest the potential of the extract as a natural active ingredient in the formulation of plant-based larvicides, contributing to environmentally friendly and sustainable dengue vector control strategies.
The Role of Telomerase in Celullar Senescence and Its Implications For Anti-Aging Therapy Defi, Ratna Shintia; Ernestia, Henrita; Pukan, Ferdinandus Krisna
Biofaal Journal Vol 6 No 1 (2025): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v6i1pp20-28

Abstract

Aging is a complex biological process influenced by genetic, environmental, and lifestyle factors. One of the primary mechanisms contributing to aging is telomere shortening, which plays a crucial role in genome stability and cellular regeneration. As individuals age, progressive telomere shortening can trigger cellular apoptosis and tissue dysfunction, impacting health and skin aesthetics. Consequently, telomere-based anti-aging therapies have become a significant focus of strategies aimed at slowing the aging process and improving quality of life. Several studies have explored various therapeutic approaches, including telomerase activation, bioactive compounds, and genetic engineering technologies to maintain telomere length and enhance cellular function. This literature review aims to analyze recent developments in telomere-based anti-aging therapies, evaluate their effectiveness, and discuss their potential applications in health and aesthetic medicine. A deeper understanding of telomere mechanisms and their therapies is expected to facilitate the development of more effective and sustainable anti-aging strategies.
Therapeutic Effect of Pulai Bark Extract (Alstonia scholaris L. R. Br) on Body Weight of Streptozotocin-Induced Mice (Mus musculus) Assagaf, Abdur Rahman; Tarigan, Ulva Karolina
Biofaal Journal Vol 6 No 1 (2025): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v6i1pp29-38

Abstract

Type 1 and type 2 diabetes mellitus are metabolic disorders characterized by elevated blood sugar levels (hyperglycemia) due to the body’s inability to produce insulin effectively. Sustained hyperglycemia can affect various physiological parameters, including changes in body weight. Alstonia scholaris L. R. Br is a plant widely found in Southeast Asia. Alstonia scholaris stem bark extract contains bioactive compounds of flavonoids, saponins, and polyphenols that can be antidiabetic. This study aims to determine the therapeutic effect of pulai bark extract (Alstonia scholaris L. R. Br) on the body weight of mice (Mus musculus) induced by Streptozotocin. This research is experimental. A total of 18 mice were divided into 6 groups, namely the normal control group (KN), negative control group (K-), positive control group (K+), and DM mice group given the extract at a dose of 0.4 ml/mencit/day (P1), a dose of 0.8/ml/mencit/day (P2) and 1.6 ml/mencit/day (P3). Before treatment, all mice were weighed, and initial blood sugar levels were. After that, the K-, K+, P1, P2, and P3 groups were induced with streptozotocin mice at a dose of 0.3 ml for 14 days. Furthermore, suppose there is an increase in blood sugar levels. In that case, the K+ group is given the drug metformin, and the P1, P2, and P3 groups are given Alstonia scholaris stem bark extract at a predetermined dose. Data from the study were analyzed using an ANOVA test. The results showed that giving Alstonia scholaris stem bark extract can increase the body weight of DM mice.
Expresion of Tumor Necrosis Factor Alfa (TNF-α) in The Pancreas of Diabetic Mice (Mus musculus) Following Adminstration of Catharantus roseus Extract Kaliky, Nunun Ainun Putri Sari Banun; Moniharapon, Mechiavel; Eddy, La
Biofaal Journal Vol 6 No 1 (2025): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v6i1pp10-19

Abstract

Catharanthus roseus is a plant that contains compounds such as alkaloids, flavonoids, saponins, and carotenoids, which act as antioxidants. This study aimed to investigate the effect of Catharanthus roseus extract on the reduction of TNF-α expression in the pancreas of diabetic mice (Mus musculus). In this study, mice with an average body weight of 30 g were induced with streptozotocin (0.1 ml/BB) to induce diabetes mellitus. Subsequently, the diabetic mice were administered Catharanthus roseus extract, using methanol and petroleum benzene solvents at a concentration of 5%, with a dose volume of 1.5 ml/BB, for 14 days. On the final day, the mice were sacrificed, and the pancreas organs were collected, followed by histological preparation using immunohistochemistry methods. The results indicated that administration of Catharanthus roseus extract with methanol and petroleum benzene solvents reduced TNF-α expression. The Catharanthus roseus extract with methanol solvent was more effective in lowering TNF-α expression.
Carcass and Prime Cut of Brahman Cross Steers at Different Age Levels Permata, Anggun Sari; Hasman, Hasman; Hasrin, Hasrin; Hidayah, Asmaul Fitria Nur; Wulandari, Sri Helda; Syamsuddin, Syamsuddin; Latief, Muhammad Fadlirrahman
Biofaal Journal Vol 6 No 1 (2025): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v6i1pp39-46

Abstract

This study aimed to evaluate the effect of age on carcass yield and prime cuts in steer Brahman Cross (BX) cattle. The research was conducted from September 2 to 22, 2015, at PT Cianjur Arta Makmur slaughterhouse, Cianjur Regency, West Java. A total of 68 steer BX cattle were used, divided into four age groups based on permanent incisor eruption: PI0 (<18 months, n=15), PI2 (18–24 months, n=19), PI4 (>24–36 months, n=21), and PI6 (>36–42 months, n=13). Measured variables included slaughter weight, hot carcass weight, cold carcass weight, carcass percentage, and prime cut yields (tenderloin, striploin, cube roll). Data were analyzed using one-way ANOVA. The results showed no significant differences (p>0.05) among age groups in terms of slaughter weight (507.07 ± 44.58 kg), hot carcass weight (287.97 ± 23.85 kg), cold carcass weight (273.31 ± 23.57 kg), carcass percentage (53.95 ± 1.92%), and prime cut weights: tenderloin (3.73 ± 0.39 kg), striploin (10.27 ± 1.28 kg), cube roll (5.01 ± 0.61 kg). Although age did not significantly affect carcass and cut yields, the highest retail sales value was observed in PI2 cattle at Rp23,110,648. These findings suggest that while age level does not significantly influence carcass or prime cut weights, economic returns may vary based on carcass composition and retail pricing.
Effectiveness of Methanol and Petroleum Benzene Extracts of Catharantus roseus Leaves in Reducing Blood Sugar Levels in Mice (Mus musculus) Diabetes Mellitus Model Siscawati, Winda; Baszary, Chomsa Dintasari Umi
Biofaal Journal Vol 6 No 1 (2025): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v6i1pp47-59

Abstract

Catharanthus roseus, a plant belonging to the Apocynaceae family, contains various chemical compounds. The roots, stems, leaves, and seeds of Catharanthus roseus contain over 70 types of alkaloids, including 28 bi-indole alkaloids found throughout the plant. These compounds, many of which are beneficial in medicine, exhibit blood sugar-lowering (hypoglycemic) properties. This study aims to evaluate the effects of pure methanol extracts of Catharanthus roseus leaves on diabetes mellitus in mice, providing comprehensive evidence of its efficacy. Methanol and petroleum benzene extracts of Catharanthus roseus leaves were diluted to a 5% concentration and administered to test animals at a dose of 1.5 ml. After a two-week testing period, the results showed a significant reduction in blood sugar levels in the diabetes mellitus model mice. This demonstrates the hypoglycemic effect of methanol and petroleum benzene extracts of Catharanthus roseus leaves in lowering blood glucose levels in streptozotocin-induced mice.
A Case Report on Internal Bleacing of a Discolored Central Incisor Using 35% Hydrogen Peroxide Sutowijoyo, Aryo; Nerito, Prima
Biofaal Journal Vol 6 No 1 (2025): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v6i1pp60-66

Abstract

A patient presented with tooth discoloration due to trauma, which had caused a tooth to become non-vital. Such trauma can damage the pulp tissue, eventually leading to necrosis and discoloration over time. In cases like this, internal bleaching is a treatment procedure aimed at improving the color of non-vital teeth. Discoloration of teeth after endodontic treatment often raises concerns for both patients and dentists. Whitening the tooth after root canal treatment is important to restore its original color before a permanent restoration is placed. The objective of this report is to present the successfull outcome of internal bleaching in patient who had undergone root canal treatment en years earlier. A 39-year-old male presented with a complaint of discoloration in the right maxillary central incisor. The patient had a history of root canal treatment on the tooth performed ten years prior. Radiographic examination confirmed that tooth 11 had undergone root canal treatment. Internal bleaching was performed on tooth 11, which appeared darker than the adjacent teeth. Whitening was carried out using 35% hydrogen peroxide. By the fourth visit (one week after the third application), the tooth color had changed to shade D3, which matched the desired color. This indicated a significant improvement in shade. Internal bleaching using 35% hydrogen peroxide resulted in color enhancement consistent with patients expectations. The treatment proved effective in restoring the esthetics of a non-vital tooth discolored due to trauma.

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