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Biofaal Journal
Published by Universitas Pattimura
ISSN : -     EISSN : 27234959     DOI : https://doi.org/10.30598/biofaal.v1i1y2020
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Materials Science & Nanotechnology Medicine & Pharmacology
Biofaal Journal merupakan jurnal online yang menerima dan mempublikasikan tulisan-tulisan ilmiah dalam bentuk artikel hasil penelitian terbaru, short communication, maupun review terhadap artikel dari bidang keilmuan Biologi, Faal Tumbuhan, Faal Hewan, Faal Manusia dan Faal Olahraga. Biofaal Journal diterbitkan oleh Profesi Ahli Ilmu Faal, Jurusan Biologi FMIPA Universitas Pattimura. Dalam setahun Biofaal Journal memiliki 1 volume dan 2 Issue. Dewan editor, berasal dari rumpun ilmu biologi dan fisiologi yang aktif dalam publikasi ilmiah skala nasional dan internasional, sehingga mampu melakukan proses review secara profesional. Artikel yang diterima akan diterbitkan sesuai dengan waktu yang ditentukan yaitu pada bulan Juni dan Desember, dan akan tersedia gratis bagi pembaca
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 83 Documents
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UJI AKTIVITAS ANTIBAKTERI Vibrio sp. DENGAN EKSTRAK ETANOL DAUN SIRSAK (Annona muricata L.) Sianturi, Anggreni; Hitijahubessy, Hendro; Rumangun, Anastasia Putri Marshanda; Samid, Aprianti
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp072-080

Abstract

Study of the antibacterial ability of soursop leaf extract (Annona muricata L.) in inhibiting the growth of Vibrio sp. The maceration method with 70% ethanol solvent was used in this research to determine the yield, then the extract was diluted in stages from a concentration of 100% to 3.125%. The antibacterial test was done using the disc diffusion method in TCBS media and a phytochemical test was carried out on soursop leaves to analyze the secondary metabolite content. The extract yield obtained in this study was 3.77%. The test results showed that the largest zone of inhibition was found at 100% extract concentration of 11.45mm. The smallest inhibition zone was found in the 6.25% extract with an inhibition zone of 1.44mm, the diameter of the positive control (tetracycline) inhibition zone was 22.75mm, the diameter of 10% DMSO as a negative control was 0 mm. Phytochemical tests of soursop leaves show that they contain alkaloids, flavonoids, steroids, saponins and tannins.
EFEKTIFITAS ANTIBAKTERI Vibrio sp. DARI EKSTRAK DAUN MANGROVE Rhizopora apiculata Hitijahubessy, Hendro; Irmawati, Yuni
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp081-089

Abstract

Research was carried out by analyzing the antibacterial ability of the ethanol extractof mangrove Rhizopora apiculata leaves against Vibrio sp. Maceration using 70%ethanol resulted in an extract yield of 3.09%. The highest average inhibition zoneresults were found in mangrove leaf extract at a concentration of 100% with aninhibition zone of 24.68 mm. The smallest inhibition zone was found in mangrove leafextract with a concentration of 6.25% with an inhibition zone of 3.85 mm, a positivecontrol inhibition zone (30 mg tetracycline) of 26.35 mm and a negative controlinhibition zone (DMSO 10%) of 0 mm. This can be seen from the results of theaverage inhibition zone which shows strong antibacterial activity of mangrove leafextract at a concentration of 100% and moderate antibacterial activity of mangroveleaf extract at a concentration of 75% to 50% and no antibacterial activity atconcentrations of mangrove leaf extract below 25 %, the positive control inhibitionzone (30 mg tetracycline) was 26.35 mm and the negative control inhibition zone(DMSO 10%) was 0 mm. Phytochemical analysis was also carried out on mangroveleaves with the results showing the presence of several visible secondary metabolites.The secondary metabolites found in Rhizopora apiculata mangrove leaves consist ofalkaloids, flavanoids, steroids, saponins and tannins.
UJI PURI SEBAGAI GAMBARAN STATUS HIDRASI JANGKA PENDEK SELAMA JAM KULIAH PADA MAHASISWA Herawati, Elysabet; Wijayanti, Endah Tri
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp090-099

Abstract

The large number of activities without being balanced with sufficient consumption of drinking water can affect the hydration status of the body, namely the occurrence of dehydration. The study aims to describe the short-term hydration status of D-III Nursing Study Program students in terms of the PURI test results. The method used is the PURI test, namely the urine self-check method which is carried out in 2 tests. The research also used a survey method to support the results of the PURI test. Based on the results of the study, the short-term hydration status of D-III Nursing Study Program students based on the PURI test on the results of the 1st test showed that most of the students 15% experienced mild dehydration and 72% experienced severe dehydration. This is related to the habit of students who never bring drinking water to campus and most students do not consume enough water during the range of lecture hours until the lecture is over. The results of the 2nd PURI test showed that 76% of students had good hydration status because prior to testing students were encouraged to consume sufficient amounts of water. The results of the study show that students awareness of meeting body fluids to maintain body fluid homeostasis needs to be increased.
ANALISIS KEMAMPUAN DARI EKSTRAK ETANOL RUMPUT LAUT Ulva lactuca SEBAGAI ANTIBAKTERI Vibrio sp. DAN KAJIAN FITOKIMIANYA Metungun, Jakomina; Beruatjaan, Mariana Yerminal; Hitijahubessy, Hendro; Tamher, Endang
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp100-107

Abstract

An analysis of the antibacterial ability of Vibrio sp. has been carried out from the ethanol extract of Ulva lactuca seaweed and its phytochemical study. Analysis of the antibacterial ability of Vibrio sp. using the disk diffusion method was the main thing carried out in this research, then the extract yield was calculated from the maceration process of Ulva lactuca seaweed, as well as phytochemical analysis to support the antibacterial ability of Ulva lactuca seaweed against the bacteria Vibrio sp. The results of the extraction of Ulva lactuca seaweed with ethanol solvent obtained a yield of 3.67%. The results showed a moderate antibacterial activity of Vibrio sp. from Ulva lactuca seaweed extract at a concentration of 100% was 17.3 mm. Weak antibacterial activity was at a concentration of 75% of 12.525 mm and extracts below a concentration of 75% did not have inhibitory power against Vibrio sp bacteria. Phytochemical studies of Ulva lactuca seaweed found secondary metabolite compounds in the form of alkaloids, flavonoids, steroids, tannins and saponins. Based on the results of this research, it is hoped that the antibacterial ability of Vibrio sp. can be further investigated from ethanol extract of Ulva lactuca seaweed in vivo.
ANALISIS FILOGENETIK GEN gyrA DAN gyrB DARI GENUS MESORHIZOBIUM, RHIZOBIUM DAN ENSIFER Apituley, Edwin Thomas; Samson, Efraim; Killay, Amos
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp118-127

Abstract

Nucleotide sequence of gyrA and gyrB genes encoding subunit A and B of gyrase enzyme from genus of Rhizobium, Ensifer and Mesorhizobium were analyzed to determine evolutionary relationship among them. Maximum Likelihood and Kimura 2 parameter methods were used to construct phylogenetic tree and measure genetic distance. Phylogenetic tree based on nucleotide sequence of gyrA gene was compared to phylogenetic tree based on nucleotide sequence of gyrB gene. Comparison of identity percentage among nucleotide sequence of gyrA gene from genus Rhizobium, Ensifer and Mesorhizobium show similarity, where each of them have narrow range of identity percentage, however nucleotide sequence of gyrB gene from genus Rhizobium and Ensifer show wider identity percentage range than genus Mesorhizobium. Several member of genus Rhizobium and Ensifer have double copy of gyrB gene with identity percentage less than 50 percent between them. Topology of phylogenetic tree based on nucleotide sequence of gyrA gene have similar topology to topology of phylogenetic tree based on nucleotide sequence of gyrB gene, except for additional branch formed by one of additional copy of gyrB sequences. .
POTENSI KULIT BATANG Cinnamomum burmannii Bl. DALAM MENCEGAH INFERTILITAS; KAJIAN TERHADAP BERAT TESTIS DAN JUMLAH SPERMATOZOA MENCIT (Mus musculus) MODEL DIABETES MELLITUS TIPE-1 Moniharapon, Mechiavel; Ukratalo, Abdul M; Pattimura, Nadira; Samson, Efraim; Pangemanan, Victory O
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp108-117

Abstract

In individuals with diabetes mellitus, the increase in Reactive Oxygen Species (ROS) can damage the mitochondrial membrane, leading to the loss of potential mitochondrial membrane function in inducing apoptosis of sperm cells. Cinnamomum burmannii is a type of spice that contains antioxidants, thus capable of neutralizing free radicals and reducing oxidative stress caused by diabetes mellitus. Flavonoids also have an effect on increasing spermatogenesis. The aim of this research is to examine the role of methanol extract from Cinnamomum burmannii bark in increasing testis weight and spermatozoa count in type-1 diabetes mellitus mice (Mus musculus). The study employs a Completely Randomized Design (CRD) with five treatments and three replications. Twenty mice are divided into groups: K- (control), K+ mice injected with STZ without medication), P1 (dose of 200 mg/kg BW), P2 (dose of 400 mg/kg BW), and P3 (dose of 800 mg/kg BW). Before administering the Cinnamomum burmannii bark extract, blood sugar levels are measured in the K+, P1, P2, and P3 groups. If blood sugar levels have increased (>128 mg/dL), the P1, P2, and P3 groups are given the Cinnamomum burmannii bark extract according to the predetermined dosage. The treatment is carried out for 14 days. On the last day, surgery is performed to extract the testes and observe the spermatozoa count. The observation data on testis weight and spermatozoa count will be analyzed using ANOVA with SPSS 24.0. The results indicate that the administration of methanol extract from Cinnamomum burmannii bark significantly affects the increase in testis weight and spermatozoa count in type-1 diabetes mellitus mice. The increase in testis weight and spermatozoa count is proportional to the dosage given
PERUBAHAN SIFAT-SIFAT KIMIA PADA TANAH ULTISOL PASCA PEMBERIAN PENGAPURAN DAN KOTORAN KELELAWAR, SERTA EFEKNYA TERHADAP HASIL BIJI KERING KACANG TANAH (Arachis hypogeae L.) Charles Silahooy; Adrien Jems Akiles Unitly; Veince Benjamin Silahooy; Efraim Samson; Edwin T Apituley
Biofaal Journal Vol 5 No 1 (2024): Biofaal Journal
Publisher : Jurusan Biologi FMIPA Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v5i1pp001-009

Abstract

Penelitian ini bertujuan untuk mengetahui perubahan sifat-sifat kimia pada tanah ultisol pasca pemberian pengapuran dan kotoran kelelawar, serta efeknya terhadap hasil biji kering kacang tanah (Arachis hypogeae L.). Penelitian ini dilaksanakan pada rumah plastik di Desa Ouw, Kabupaten Maluku Tengah, berlangsung dari bulan Oktober 2023 sampai Maret 2024. Analisis sifat-sifat tanah dilakukan di Laboratorium Tanah, Fakultas Pertanian Universitas Pattimura. Rancangan percobaan yang digunakan dalam penelitian ini adalah Rancangan Acak Langkap (RAL) berpola faktorial 4 x 4 x 4, dimana parameter yang diteliti adalah pH tanah (H20 1 : 2,5), kemasaman dapat tukar (Aldd + Hdd), Kadar Cadd, Kadar Mgdd dan berat biji kering per tanaman. Hasil penelitian menunjukkan bahwa peranan kapur terutama dalam menaikkan pH tanah dan menurunkan kemasaman dapat tukar (Aldd + Hdd), sedangkan peranan kotoran kelelawar terutama dalam menaikkan kadar Cadd, Mgdd dan hasil biji kering kacang tanah pada tanah ultisol. Selain itu, Hasil setara biji kering kacang tanah terbaik (18,58g / tanaman) diproleh pada taraf kotoran kelelawar 192g per pot tanpa pengapuran pada tanah ultisol dengan pH tanah 5,3 dan kejenuhan Al 66,22%.
JUMLAH SEL LEYDIG DAN SEL SERTOLI TIKUS GALUR SPARAGUE-DAWLEY TERPAPAR SOPI PASCA DITERAPI EKSTRAK ETANOL SIRIH CINA (Peperomia pellucida L.) Adrien Jems Akiles Unitly; Amos Killay; Mechiavel Moniharapon; La Eddy; Veince B Silahooy; Laury Marcia Ch Huwae; Debby Dijola Moniharapon; Bella Frida Lakesubun
Biofaal Journal Vol 5 No 1 (2024): Biofaal Journal
Publisher : Jurusan Biologi FMIPA Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v5i1pp010-018

Abstract

Tujuan penelitian ini untuk mengetahui jumlah sel Leydig dan sel Sertoli tikus terpapar sopi pasca diterapi ekstrak etanol sirih cina (Peperomia pellucida. L). Rancangan acak lengkap (RAL) digunakan dalam penelitian ini, dengan membagi 15 ekor tikus ke dalam 5 kelompok perlakuan yang diulang sebanyak 3 kali, yaitu kelompok 5.4 adalah 3 ekor tikus yang diberi sopi 5.4 ml/ekor/hari selama 14 hari (kontrol negatif), kelompok Vit. C 6.3 adalah 3 ekor tikus yang diberi sopi 5.4 ml/ekor/hari kemudian diberi Vitamin C 6.3 mg/ekor/hari selama 14 hari (kontrol positif), kelompok 0.71, 1.43 dan 2.86 adalah 3 ekor tikus yang diberi sopi 5.4ml/ekor/hari selama 14 hari kemudian kemudian masing-masing kelompok diberi ekstrak etanol sirih cina 0.71g/ekor/hari selama 14 hari, 1.43g/ekor/hari selama 14 hari dan 2.86g/ekor/hari selama 14 hari. Penelitian ini dilakukan di Laboratorium Zoologi dan Mikroteknik Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Pattimura Ambon mencakup persiapan hewan model, pemberian minuman sopi, ekstraksi sirih cina, pembuatan preparat histologis dan pengamatan. Hasil yang diperoleh dianalisis dengan Analysis of Variance (ANOVA) dan dilanjutkan dengan uji duncan pada taraf nyata a = 0.05 menggunakan perangkat lunak SAS dan dilanjutkan dengan uji beda nyata terkecil untuk mengetahui perbedaan perlakuan yang diberikan. Hasil penelitian menunjukan bahwa pemberian sopi 5.4 ml dapat menyebabkan penurunan jumlah sel Leydig dan sel Sertoli dan setelah diberi ekstrak etanol sirih cina, jumlah sel Leydig dan sel Sertoli mengalami peningkatan, dimana dosis ekstrak etanol sirih cina yang baik untuk sel Leydig adalah 2.86g dan untuk sel Sertoli adalah dosis 1.43g.
JENIS-JENIS TUMBUHAN ANGGOTA FAMILI ARECACEAE DI DESA SOYA KOTA AMBON DAN PEMANFAATANNYA Yulyanti Natasya Teusiit; Evelin Tuhumuri; Dece Elisabeth Sahertian
Biofaal Journal Vol 5 No 1 (2024): Biofaal Journal
Publisher : Jurusan Biologi FMIPA Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v5i1pp026-033

Abstract

Tumbuhan famili Arecaceae atau yang umum dikenal di Indonesia dengan nama palem banyak dimanfaatkan untuk memenuhi kebutuhan hidup masyarakat, baik sebagai sumber pangan, papan maupun sebagai tanaman hias. Penelitian ini dilakukan untuk menginventarisir anggota famili Arecaceae yang terdapat di Desa Soya Kota Ambon dan mendeskripsikan pemanfaatannya oleh masyarakat setempat. Survei lapangan dilakukan dengan metode jelajah untuk mendata tumbuhan famili Arecaceae. Wawancara dilakukan dengan masyarakat setempat untuk mengetahui pengenalan masyarakat terhadap anggota famili Arecaceae dan pemanfaatannya. Hasil penelitian menunjukkan bahwa terdapat delapan jenis tumbuhan anggota Arecaceae yaitu kelapa (Cocos nucifera), enau (Arenga pinnata), sagu (Metroxylon sagu), salak (Salacca edulis), pinang (Areca catechu), palem merah (Cyrtostachys renda), palem kuning (Dypsis lutescens), dan palem botol (Hyophorbe lagenicaulis). Tumbuhan anggota Arecaceae yang ditemukan di Desa Soya dimanfaatkan oleh masyarakat sebagai bahan makanan dan minuman, bahan bangunan, peralatan rumah tangga, asesoris dan tanaman hias.
PENGARUH WAKTU PEMANGKASAN DAUN DIBAWAH TONGKOL TERHADAP PRODUKSI TANAMAN JAGUNG (Zea mays L) DI DESA PADANG KECAMATAN BINTAUNA BOLAANG MONGONDOW UTARA Masshynio Ch. A. Noeng; Adeleyda M. W. Lumingkewas; Jemmy Najoan; Arthur Pinaria; Selvie Tumbelaka; Sofia Wantasen; Sandra Pakasi
Biofaal Journal Vol 5 No 1 (2024): Biofaal Journal
Publisher : Jurusan Biologi FMIPA Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v5i1pp019-025

Abstract

Jagung (Zea mays L) adalah salah satu tanaman pangan yang merupakan sumber karbohidrat utama kedua setelah beras. Metode modifikasi lingkungan mikro dapat meningkatkan produksi jagung. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh waktu pemangkasan daun di bawah tongkol terhadap tingkat produksi jagung. Studi ini dilakukan di Desa Padang, Kecamatan Bintauna, Kabupaten Bolaang Mongondow Utara, Sulawesi Utara, dari Desember 2023 hingga April 2024. Metode penelitian adalah rancangan acak kelompok dengan lima perlakuan dan tiga kali ulangan. Penelitian menemukan bahwa perlakuan pemangkasan daun di bawah tongkol adalah tanpa pemangkasan (P0), pemangkasan 60 hari setelah tanam (P1), pemangkasan 70 hari setelah tanam (P2), pemangkasan 80 hari setelah tanam (P3) dan pemangkasan 90 hari setelah tanam. Hasil penelitian menunjukkan bahwa komponen produksi pada panjang tongkol, yaitu ukuran tongkol 19,26 cm dengan diameter 5,00 cm, berat biji sekitar 241.92 g, dan jumlah biji sekitar pertongkol 617.42, meskipun berdasarkan hasil analisis sidik ragam, pengaruh tanaman jagung hanya terbatas pada panjang dan diameter tongkol.

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