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Ivandini Tribidasari A.
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Makara Journal of Science
Published by Universitas Indonesia
ISSN : 23391995     EISSN : 23560851     DOI : https://doi.org/10.7454/mss
Core Subject :
Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and Molecular Biology (including: microbiology, physiology, ecology, taxonomy and evolution); and Biotechnology.
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Articles 880 Documents
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Sequence and Phylogenetic Analysis of Citrus maxima (Burm.) Merr. from Tomini Bay, Sulawesi Island, Based on the Maturase K Gene Zusriadi, Brenda Febrina; Kandowangko, Novri Youla; Febriyanti, Febriyanti
Makara Journal of Science Vol. 29, No. 3
Publisher : UI Scholars Hub

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Abstract

This study was conducted in the coastal area of Tomini Bay, Sulawesi Island, and it focused on Citrus maxima, a plant known for its unique fruit flesh colors, which range from yellow to pink, and its varying leaf stalk wings. The study aimed to analyze the variations in maturase K (matK) sequences, molecular characteristics, and phylogenetic relationships of two C. maxima samples from Tomini Bay compared to other C. maxima and Citrus species using data available in GenBank. The study utilized DNA barcoding with matK molecular markers, followed by phylogenetic tree construction using the maximum likelihood method and the Tamura 3-parameter model. The matK DNA sequences from both samples were 1,530 base pairs in length, with an AT content of 64.64%, a GC content of 35.36%, and no variable sites. Phylogenetic tree reconstruction revealed that C. maxima formed a monophyletic group. These samples were also grouped in the same phylogenetic branch as C. maxima Mato Buntan, C. maxima Suisho Buntan, and C. maxima Banpeiyu, with a similarity index score of 100%, which indicates a very close relationship. Despite morphological differences, genetic analysis showed significant similarities among the samples, confirming a close relationship with minimal genetic variation
Detection Method for Escherichia coli Using Real-Time Polymerase Chain Reaction Targeting the yhaV Gene Nurjayadi, Muktiningsih; Fitriyanti, Anisa; Musie, Royna Rahma; Putri, Gusti Angieta; Azizah, Puan Aqila; Angelina, Helzi; Grace, Grace; Sihombing, Ananda Indah Putri; Setiawan, Agus; Declan, Jefferson Lynford; Putri, Gladys Indira; Juliansyah, Dandy Akbar; Fatimah, Siti; Berkahingrum, Ayu; Kartika, Irma Ratna; Kurniadewi, Fera; Saamia, Vira; Chen, Shyi-Tien; Aboemolak, Bassam; Enshasy, Hesham Ali El
Makara Journal of Science Vol. 29, No. 3
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Escherichia coli is a foodborne pathogenic bacterium that can cause diarrhea, while yhaV is a virulence-associated gene linked to the toxin–antitoxin system in E. coli. This study was aimed at evaluating the confirmation, specificity, and sensitivity of a yhaV gene primer using real-time polymerase chain reaction. The yhaV-targeting PCR successfully amplified a DNA fragment with an amplicon length of 207 bp (base pairs) under an annealing temperature optimized to a range of 54 °C to 62 °C via gradient PCR. The PCR using the primer pair produced a consistent Ct (cycle threshold) of 14.14 ± 0.05 and showed a single peak in the melting curve at a Tm (melting temperature) of 83.67 °C ± 0.02. The specificity test indicated that the yhaV primer effectively distinguished E. coli from nontarget bacteria on the basis of differences in Ct and Tm values. The sensitivity analysis showed that the PCR directed toward the primer pair successfully detected E. coli at a minimum concentration of 2.24 pg/µL, with a Ct value of 29.93 and a detection limit of 31.5 × 102 CFU. These results suggest that yhaV-based real-time PCR quickly and accurately identifies E. coli. Primer designs that target yhaV have the potential to be developed as components of a rapid, specific, and sensitive kit for detecting E. coli in food samples.
Effect of Varying Chitosan Molecular Weights on the Morphology and Physical Characteristics of PVA/Chitosan Composite Hydrogels Sihombing, Yuan Alfinsyah; Luthfianti, Halida Rahmi; Waresindo, William Xaveriano; Rodhiyah, Marathur; Nafisah, Nur’aini; Hapidin, Dian Ahmad; Edikresnha, Dhewa; Khairurrijal, Khairurrijal
Makara Journal of Science Vol. 29, No. 3
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Abstract

We employed the innovative freeze–thaw method to investigate the effect of chitosan (CS) molecular weights incorporated into polyvinyl alcohol on the morphology and physical characteristics of the hydrogel. This study aimed to unravel the intricate relationships between CS molecular weight variations, CS concentration, and the number of freeze–thaw cycles (4 or 6 cycles) on hydrogel swelling degree and weight loss. Incorporating CS improved the hydrogel structure and enhanced its porosity, resulting in remarkable increases in swelling degree ranging from 2 to 6 times the initial weight. Higher molecular weight CS (310,000–375,000 Da) exhibited a lower swelling degree than medium molecular weight CS (190,000–310,000 Da), attributed to its ability to prevent crystal formation during the freeze–thaw process. Increased freeze–thaw cycles led to a decreased swelling degree, which indicated enhanced rigidity and stability due to more significant hydrogen bond formation with hydroxyl groups. Higher CS content amplified hydrogel weight loss, and additional cycles accentuated this effect, highlighting the challenge of maintaining an effective gel structure. CS with medium molecular weight emerged as the more suitable choice for applications demanding superior water absorption.
Synthesis, Molecular Characterization, and Antibacterial Testing of Silver–Cadmium Bimetallic Bionanoparticles Prepared from the Sidaguri (Sida rhombifolia L.) Extract Mandey, Fredryk; Rangga Bua, Anita Rahelea; Rahayu, Ismi Sri; Permatasari, Nur Umriani
Makara Journal of Science Vol. 29, No. 3
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Abstract

Herein, we successfully synthesized, characterized, and examined the antibacterial activity of silver–cadmium bimetallic bionanoparticles prepared by mixing the aqueous extract of Sidaguri (Sida rhombifolia L.) and mixtures of silver nitrate and cadmium nitrate (yield = 0.2035 g). Ultraviolet–visible spectroscopy, Fourier transforms infrared spectroscopy, X-ray diffraction, scanning electron microscopy–energy dispersive X-ray spectroscopy, and particle-size analysis, revealed that the products exhibited a maximum absorption wavelength of 427.5 nm with an absorbance intensity of 0.790; a face centered cubic structure; Ag and Cd mass percentages of 98.52% and 1.28%, respectively; an average molecular diameter of 43.8 nm, and a polydispersity index of 0.573. The bimetallic nanoparticles were tested as an antibacterial agent against Staphylococcus aureus and Escherichia coli. They exhibited inhibition zone diameters of 9.2 and 10 mm for E. coli and S. aureus, respectively.
Antibacterial Activity of ZnO/Geopolymer Composite Granules and Their Potential Application in Water Treatment Maulitia, Ziyan Tirta; Angelina, Putri Nur; Sari, Pipit Erlita; Sugiarti, Sri; Arief, Irma Isnafia; Abidin, Zaenal
Makara Journal of Science Vol. 29, No. 3
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Abstract

Water pollution has become a major global concern owing to its complexity and widespread impact. Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) are the most common bacterial contaminants in water sources, which pose significant threats to public health. To mitigate water contamination by these pathogenic microorganisms, developing and implementing effective water treatment technologies are essential. A promising approach involves using antibacterial water filtration systems. Herein, zinc oxide (ZnO) nanoparticles were synthesized via hydrothermal and precipitation methods as antibacterial agents for water treatment. These preparation methods required considerably low synthesis time. A capping agent was used to control nanoparticle growth and produce small nanoparticles. The synthesized ZnO nanoparticles were added in the surface of geopolymers to obtain ZnO/geopolymer composite granules, which improved their suitability for water filtration. The antibacterial activity of the ZnO nanoparticles and their composite granules was evaluated. Results indicated that synthetic ZnO exhibited superior antibacterial performance than commercial ZnO against S. aureus (gram-positive) and E. coli (gram-negative). Furthermore, incorporating either commercial or synthetic ZnO into the geopolymer matrix moderately enhanced the antibacterial activity of the resulting material. Notably, the addition of as low as 5 wt.% ZnO was sufficient to significantly improve the antibacterial efficacy of the composite. This finding highlights the potential of ZnO in water treatment applications.
The Effect of Ozone Treatment on the Quality of Biodiesel from Waste Cooking Oil Setyawati, Sri Redjeki; Wibowo, Singgih
Makara Journal of Science Vol. 29, No. 3
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This research demonstrates the successful production of biodiesel from waste cooking oil (WCO) using the transesterification method. A methoxide solution, formed by employing a base catalyst and alcohol, facilitated the conversion of triglycerides into methyl esters and glycerol as the byproduct. The conversion process yielded 74% biodiesel and 13.5% glycerol content, with the remaining proportion consisting of water and contaminants. The application of ozone (O3) aeration proved effective in reducing contaminants—such as alcohol, unreacted catalysts, food residues and water—by approximately 60%. Furthermore, Fourier transform infrared (FTIR) testing affirmed that the biodiesel product—treated with O3 aeration—maintained its original characteristics, thus ensuring the product’s quality. In conclusion, this study highlights the potential of biodiesel production from WCO and emphasizes the importance of O3 aeration in enhancing product purity. This finding is crucial for scaling up the biodiesel production process for broader industrial applications.
The Quality of Bio-pellets Made by Combining Walnut Kernel Shells (Canarium Indicum L.) from Bogor, Indonesia, and Palm Kernel Shells as Renewable Energy Iryani, Ani; Kusumawardani, Linda Jati; Juniansyah, Ronaldo
Makara Journal of Science Vol. 29, No. 3
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Using biomass as an energy source helps overcome the declining supply of fossil fuels. Bio-pellets—an energy product made from biomass—is one option that can be utilized. One source of biomass energy is walnut kernel shells. The walnut plant (Canarium indicum L.) is a forest tree grown in eastern Indonesia. The walnut plant is also located in the GOR Pajajaran area of Bogor City and has not been utilized for energy production. This study consists of three stages: 1) raw material preparation, 2) bio-pellet production, and 3) bio-pellet characterization in accordance with the Indonesian National Standard (SNI) 8951:2020. Based on the results of our study, bio-pellets made from 100% walnut kernel shells can be utilized as fuel, with a calorific value of 4,593 cal/g. Although the moisture content remains high, the calorific value still meets the standard for power plants according to SNI 8951:2020. In addition, palm kernel shells can improve the quality of bio-pellet products. The combination of 30% walnut kernel shells and 70% palm kernel shells improves calorific value, with the following specifications: 4,684.4 cal/g of calorific value, 8.83% moisture content, 1.85% ash content, 69.16% volatile materials, 19.30% fixed carbon, 0.02% sulfur content, and 1.18% bulk density.
Employing SecA Recombinant Protein to Generate Polyclonal Antibodies for the Rapid Identification of Phytoplasma Fatinah, Arik Arubil; Rifa'i, Muhaimin; Arumingtyas, Estri Laras; Widyarti, Sri
Makara Journal of Science Vol. 29, No. 3
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Phytoplasma, a plant pathogen that threatens plant health, can induce diseases such as yellow wilt, virescence, phyllody, and witches’ broom. Although early detection methods using polyclonal and monoclonal antibodies have been developed, synthetic peptides offer advantages such as higher titers, predictable antigenic characteristics and superior sensitivity and specificity. Epitope mapping plays a key role in designing peptide antigens and includes gene synthesis, transformation, expression assessment, large-scale production, and purification. A study using New Zealand male White rabbits was conducted to generate antibodies against phytoplasma. The SecA (395–470) sequence, obtained from the preprotein translocase meets the criteria of being non-homologous, nonallergic, and antigenic. The three-dimensional structure of SecA (395–470) has a sequential similarity of 77–97% with certain phytoplasma types, and the protein purity exceeds 90%. The production of polyclonal antibodies was successful, achieving titers greater than 1:512,000. The SWISS model was used to predict the 3D structure of SecA in phytoplasma species, revealing structural homology with other phytoplasma species. The recombinant protein antigen SecA was able to induce high-titer antibody formation (>1:512.000). The developed lateral flow immunoassay (LFA) detects phytoplasma in plants using purified and conjugated antibodies. The peptide design, derived from Aster yellows witches-broom (SrI), effectively detects phytoplasma from various groups, especially Cactus witches’ broom phytoplasma (SrII-C) and Peanut witches’ broom phytoplasma (SrII-A). The SrI group phytoplasma was chosen as a reference sequence due to its` ability to infect plants across the broadest host range. LFIA was tested on samples from both phytoplasma-infected and healthy plants using nested PCR. The SecA sequence was successfully produced and used as an immunogen candidate against phytoplasmas.
Gama Melon Parfum, a Potential Melon Genotype Tolerant of Salinity Stress Nugroho, Giri; Chikmawati, Tatik; Sartika, Dian; Yusuf, Adib Fakhruddin; Putro, Karso Suryo; Latifah, Vida Rahma; Daryono, Budi Setiadi; Wibowo, Anjar Tri; Nurhayati, Awik Puji Dyah; Meitha, Karlia; Miftahudin, Miftahudin
Makara Journal of Science Vol. 29, No. 3
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Melona, Tacapa Gold, and Gama Melon Parfum are novel Melon genotypes that have been introduced for cultivation in the saline-prone coastal region of Bocor Beach, Kebumen, Central Jawa. Elevated salt concentrations in the area pose challenges, impacting yield. The potential of these new genotypes for saline land remains unexplored. This study aimed to assess the morphophysiological and molecular responses of these genotypes to salinity stress. Seeds were germinated in cocopeat and ABmix 3000 solution and treated with 220 mM NaCl until harvest. The analysis included morphology, chlorophyll content, proline accumulation, malondialdehyde levels, and gene expression were analyzed. After 1 week of salinity stress, the results showed reduced plant height, leaf number, stomatal density, percentage of opened stomata, and width and length of leaves. The malondialdehyde, proline, and root-shoot dry mass ratio increased. The expression of CSD1-1, P5CR, and late embryogenesis abundant genes increased under salinity treatment. Gama Melon Parfum exhibits salt tolerance, as indicated by higher P5CR gene expression, proline accumulation, and leaf-relative water content. P5CR gene expression, proline accumulation, and leaf-relative water content are valuable indicators of salt tolerance for assessing melon genotypes.
Synthesis and Characterization of Microcrystalline Cellulose (MCC) from Corn Waste (Zea Mays L.) Haliman, Annisa Cendani; Yuliati, Selastia; Hasan, Abu
Makara Journal of Science Vol. 29, No. 3
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Corn (Zea Mays L.) is one of the most widely cultivated food crops in the world, including in Indonesia. Corn plants produce waste, especially husks and cobs. Corn waste has no economic value and can lead to environmental pollution if burned. Corn husks contain approximately 44.08% cellulose, while cobs have a cellulose content of 45%. This high cellulose content makes it possible to process corn waste into microcrystalline cellulose (MCC). This study aims to investigate the effect of NaOH concentration and delignification time on the characteristics of the resulting MCC products. A mixture of corn husk and cob was treated with NaOH solutions of 5%, 10%, and 15% concentration, for delignification periods of 30, 60, 90, and 120 minutes, respectively. Furthermore, 10% H2O2 was used in the bleaching process to obtain alpha cellulose, followed by hydrolysis using HCl 2.5 N. Characterization of the MCC products was executed using X-ray diffraction (XRD) and scanning electron microscopy (SEM). The sample tests included organoleptic evaluations for shape, colour, and odour, as well as determination of the yield percentage, acidity, and moisture content. The crystallinity of the MCC obtained was satisfactory, while the SEM results indicated a pattern similar to Avicel pH 102. Increasing the concentration of NaOH resulted in smaller MCC particle sizes, facilitating dispersion during the acid hydrolysis. The NaOH concentration and delignification duration affected the yield percentage and surface contour, as observed in the SEM test, but the physical properties of the MCC were not affected by the concentration of NaOH. A concentration of 5% NaOH and a 90-minute delignification process yielded the highest crystallinity, at 99.4%, supported by a yield percentage of 77.09%, a pH of 5.84, a moisture content of 5.81%, and a particle size of 210.82 μm.

Page 86 of 88 | Total Record : 880

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