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INDONESIA
Jurnal Sain Veteriner
Published by Universitas Gadjah Mada
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : -
Articles 824 Documents
 57   58   59   60   61 
Efektivitas Low Density Lipoprotein (LDL) dari Kuning Telur Ayam terhadap Kualitas Semen Cair Domba Dwitya Citraesti; Wahono Esthi Prasetyaningtyas; Ni Wayan Kurniani Karja
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.63395

Abstract

Low-Density Lipoprotein (LDL) extracted from egg yolk (LDL) has recently known can eliminate the adverse effect associated in the use of fresh egg yolk. The role of LDL in liquid preservation at 4°C of ram sperm has not been explored. This research evaluates the effects of substituting egg yolk with LDL in ram sperm preservation at 4 °C on 5 days. The objective of this research was to assess the effects of substituting egg yolk with LDL for use as an extender in sperm preservation at 4°C, as well as on spermatozoa motility, viability, morphology, plasma membrane, and acrosome integrity, for 5 days. The semen was subsequently divided into five and diluted with Tris–fresh egg yolk (K), Tris–LDL5% (LDL5), Tris–LDL10% (LDL10), Tris–LDL15% (LDL15), and Tris–LDL20% (LDL20). The result showed a significant difference between LDL to fresh egg yolk for ram sperm quality (P<0.05). The effectiveness of LDL on sperm quality decreased following by its concentration. Even though up to 20% concentration of LDL, it can not preserve the quality of diluted semen for motility, viability, and plasm membrane integrity. 
Respon Imun Seluler Ayam Petelur Pascavaksinasi Avian Influenza Subtipe H5N1 Isolat dari Bali Gusti Ayu Yuniati Kencana; Tri Komala Sari; I Nyoman Suartha; I Ketut Tomy Caesar Ramanda; Anak Agung Sagung Kendran
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.66086

Abstract

Avian Influenza subtype H5N1 (AI-H5N1) is a malignant virus that is very detrimental to laying chickens because it is highly contagious and mutates easily. Prevention of AI-H5N1 disease in laying chickens is carried out by vaccination, therefore to maintain the quality of the vaccine, continuous research is needed. This study aims to determine the potential of AI-H5N1 vaccine isolates from Bali as measured based on cellular immune response based on total and differential leukocyte cells. Formation of antibodies is influenced by the nonspecific and specific immune system involving leukocytes, especially lymphocytes. Total of 40 layers of Novogen Brown strain were used for the research sample, kept since the age of one day on a commercial farm in Perean Village, Tabanan Regency, Bali. Laying chickens are vaccinated at 5 weeks of age by intramuscular injection. Total of 20 chikens were taken randomly and used for the sample. Blood draws were performed once pre-vaccination and five times each week after vaccination with anticoagulants. Total leukocytes were examined by an auto hematology analyzer, while differential leucocytes with thin blood smear stained with Giemsa. Total and differential leukocyte data were analyzed by means of the variance test followed by the Duncan test. Results showed that AI-H5N1 vaccination from Bali isolates could increase total and differential leucocytes of laying chickens and had significant effect on the mean total leukocytes, the absolute values of heterophyll cells, eosinophils, lymphocytes, and monocytes, but had no significant effect on post-vaccination basophil cells.
Daya Antelmintik Serbuk Kulit Nanas (Ananas comosus) terhadap Cacing Haemochus contortus pada Domba Dewi Pranatasari; Rido Florensius Manik; Budi Purwo Widiarso; Wida Wahidah Mubarokah
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.66420

Abstract

The problem facing sheep breeders in breeding sheep was digestive tract parasite of worm (nematodiasis and haemonchosis). Resistance to anthelmintics was the reason for the study of alternative medication of H. contortus infection. It aimed at finding out the effectiveness of the application of pineapple peel powder as H. contortus anthelmintic in sheep and the dose of the pineapple peel powder as the H. contortus anthelmintic. It used 15 sheep that were assigned to 5 groups. Group I served as positive control with the application of albendazole (Kalbazen) anthelmintic, Group II was treated using the pineapple peel powder at the dose of 150 mg/kg BW. Group III was treated using the pineapple peel powder at the dose of 200 mg/kg BW. Group IV was treated using the pineapple peel powder at the dose of 250 mg/kg BW. And, Group V served as negative control without any treatment. The treatments were conducted for 14 days and the resulting statistic data were analyzed using comparative descriptive method by comparing initial data (before the treatments) and final data (after the treatments). The comparative data showed that there was significant change in the observed variables. The results of the study showed that the pineapple peel powder could be used as the anthelmintic of the H. contortus in the sheep and the dose of 250 mg/kg BW most significantly decreased the mean number of the eggs of the worm per gram of feces
Peran Hemaglutinin dan Hemolisin pada Escherichia coli Sorbitol-negatif Isolat Burung Puyuh pada Proses Infeksi Secara in Vitro Khusnan Khusnan; Wahyu Prihtiyantoro; Dwi Kusmanto
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.69896

Abstract

Abstract Escherichia coli sorbitol-negative in quails cause economic loss due to the death, growth rate inhibition, decreased egg production, and increased medical treatment. Escherichia coli sorbitol-negative has many virulence factors, including hemagglutinin and hemolysin. The aim of this study is to determine the role of hemagglutinin and hemolysin in the infection process of Escherichia coli sorbitol-negative in vitro. This study was performed using 23 isolates of Escherichia coli sorbitol-negative from quails, 52.2% (12 of 23 isolates) had hemagglutinin, while 34.8% (8 out of 23 isolates) had hemolysin. Isolates with hemagglutinin were more attached to human buccal epithelial cells than isolates without hemagglutinin (P <0.05). Isolates with hemolysin were less phagocyted by macrophages compared to isolates which without hemolysin (P <0.05). Escherichia coli sorbitol-negative isolates from quails that have hemagglutinin and hemolysin are pathogenic isolates that possess the potential to cause colibasilosis and transmission between quails and other birds. 
Identifikasi Tipe Dehidrasi dan Profil Elektrolit Mayor pada Pasien Kucing di Rumah Sakit Hewan Prof. Soeparwi dan Beberapa Klinik Hewan di Wilayah Yogyakarta Guntari Titik Mulyani; Setyo Budhi; Kurnia .
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.69901

Abstract

Dehydration is defined as a lack of body fluids followed by loss of electrolytes, and changes in acid-base balance. The type of dehydration is limited based on the concentration of sodium in serum at the time of dehydration. Maintenance of osmotic pressure and distribution of several compartments of body fluids are the main functions of the four major electrolytes, namely sodium (Na +), potassium (K +), chloride (Cl‾), and bicarbonate (HCO3‾). Sodium is the most cation in extracellular fluid, most potassium cation in intracellular fluid and chloride is the most anion in extracellular fluid. The purpose of this study was to identify the type of dehydration and to determine the major electrolyte profile in cats in Yogyakarta and its surroundings. This study used 18 sick cats that were thought to be dehydrated, marked by decreased skin turgor, CRT> 2 seconds, and 12 cats that were suspected of having electrolyte balance disorders with symptoms of ascites, uropoetic disorders. Blood was drawn for all cats to measure Pack Cells Volume (PCV) levels. Patient clinical data and patient diagnosis were recorded, cats with changes in serum PCV levels were separated for examination of levels of sodium, chloride, potassium using Seamaty SMT-120V. The type of dehydration is identified based on the sodium level in the serum of a dehydrated cat. The results showed that most of the cat patients were dehydrated had low serum sodium levels (hyponatremia). There was 1 cat patient had low chloride levels. Potassium levels in cats with UT obstruction increased, which led to a decrease in the Na: K ratio. Cat bicarbonate levels did not show any change. From the results of the study it was concluded that dehydration in cats at Prof. Soeparwi is hypotonic dehydration (71%). The sodium profile mostly decreased, chloride and bicarbonate levels did not change, while there were changes in potassium levels in patients with UT disorders. The advice given is to check electrolytes before doing fluid therapy. Prior to electrolyte testing, dehydrated cats can be given a sodium solution. 
Identifikasi Mikroba dari Lendir Estrus dan Deteksi Kebuntingan Sapi Kawin Berulang di Sleman, Yogyakarta Surya Agus Prihatno; Abdul Samik; Dea Indriani Astuti; Muhammad Agil; Usamah Afiff; Anriansyah Renggaman; Dedi Rahmat Setiadi; Yosua Kristian Adi
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.70916

Abstract

Repeat breeding is condition of cows that have normal or near normal estrous cycles but fail to become pregnant after several insemination. In this study, we investigated the microbes in the cervical and vaginal mucus of beef cattle and dairy cows that experience repeat breeding and detected the pregnancy after the third or more artificial insemination. A total of 14 beef cattle and 6 dairy cows that experience repeat breeding in the cattle herd in the Yogyakarta region of Indonesia were used as samples in this study. Cervical and vaginal mucus samples were collected using plastic sheet when the cow was re-estrus. The samples were put into the 5 ml Brain Heart Infusion Broth for bacterial isolation and identification. Pregnancy examination was carried out on day 45 after artificial insemination using ultrasound method. Bacteria that could be isolated and identified from cervical mucus and vaginal mucus of beef cattle and dairy cows included Bacillus sp., Staphylococcus epidermidis, Staphylococcus aureus, and Pseudomonas sp. Some cows detected positive in pregnancy examination even though the bacteria were presence in the cervical and/or vaginal mucus. There was various composition of bacteria found in the cervical mucus and vaginal mucus, of beef cattle and dairy cows with repeat breeding in livestock groups in Yogyakarta. The presence of bacterial in the cervical and vaginal mucus during estrus was not always become the causes of failed pregnancy.
Mastitis di Jawa Barat, Indonesia: Etiologi dan Opsi Pencegahan Sarasati Windria
Jurnal Sain Veteriner Vol 40, No 1 (2022): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.41946

Abstract

Mastitis is Mastitis is an inflammation of the udder. Mastitis is mainly caused by pathogenic bacteria both bacterial and yeast. The purpose of this review is to describe the ecology and epidemiology of mastitis prevalent in West Java and to propose control options that may be suitable for the West Java situation. The review was conducted using published papers about mastitis in West Java and government documentation. We determined that the major classification mastitis in West Java are clinical mastitis and subclinical mastitis. We also determined that the major pathogens agent of mastitis both clinical and subclinical mastitis are bacteriology and mycology agent. Education of farmers is an important part of any control program. The ecology and epidemiology of mastitis in West Java are still largely not understood. Future studies should be aimed at the evaluation of the proposed methods of disease control, an understanding of the impact of mastitis infection on dairy production in West Java and the role of the movement of dairy cattle products into and among regions in West Java.
Improve the Performances of Broilers as Reduce Heat Stress by the Supplementation Jaloh (Salix tetrasperma Roxb) in Drinking Water Zulfan Zulfan; Sugito Sugito; Cut Aida Fitri; Herawati Latif; Khairul Murdani
Jurnal Sain Veteriner Vol 40, No 1 (2022): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.59711

Abstract

Abstrak Pencegahan dampak cekaman panas pada ayam broiler dapat dilakukan dengan pemberian ekstrak tanaman yang juga berguna dalam pengembangan obat-obat tradisional sebagai alternatif pengurangan penggunaan bahan-bahan kimiawi. Tujuan penelitian ini adalah untuk mengevaluasi performan ayam broiler yang diberikan ekstrak daun jaloh melalui air minum. Penelitian dilakukan di Laboratorium Lapangan Peternakan (LLP), Jurusan Peternakan, Fakultas Pertanian, Universitas Syiah Kuala. Sebanyak 80 ekor anak ayam broiler strain MB 202 produksi PT Japfa, Medan digunakan dalam penelitian ini. Perlakuan adalah suplementasi ekstrak daun jaloh melalui air minum pada dosis 0, 500, 1000, and 1500 ml/l air. Rancangan yang digunakan adalah Rancangan Acak Blok terdiri 4 perlakuan dan 4 blok. Tiap blok merupakan eksperimental unit yang masing-masing ditempati 5 ekor ayam. Hasil penelitian memperlihatkan walaupun secara statistik tidak berbeda nyata (P>0,05), suplementasi ekstrak daun jaloh di dalam air minum cenderung meningkatkan performan ayam broiler. Berat badan akhir, konsumsi ransum, dan konsumsi air minum meningkat sedangkan FCR menurun. Hasil penelitian disimpulkan bahwa pemberian ekstrak daun jaloh melalui air minum dapat menurunkan cekaman panas dan kematian ayam broiler dengan penggunaan terbaik pada pengenceran 1000 ml /l water air. Kata kunci: Jaloh; leaf, ekstrak, broiler, performan  Abstract Prevent heat stress in broilers has been optionally created by using plants as starting points for drug development, specifically those used in traditional medicine. The objective of this study was to evaluate the performances of broiler chickens raised by offering jaloh leaf extract (JLE) through drinking water. The research has been conducted at the Field Laboratory of Animal Husbandry, Animal Husbandry Department, Syiah Kuala University. As many as 80 broiler chicks strain MB 202 produced by PT Japfa, Medan were used in this study. The treatment was supplementation JLE within drinking water at a dose of 0, 500, 1000, and 1500 ml/l of water, respectively. The research was performed into a Block Randomized Design (RBD), consisting of 4 treatments and 4 blocks. Each block was an experimental unit, each of which was placed by 5 chickens. The results of study indicated that although statistically no significant differences were detected (P>0.05) supplementation of JLE through drinking water had tendency to improve the performances of broilers. Final body weight, body weight gain, feed intake. and drinking water intake increased and FCR decreased. It was concluded that giving JLE through drinking water could minimize heat stress and reduce the mortality of broilers with the best provision of JLE at the level of 1000 ml /l water.Key words: Jaloh; leaf, extract, broiler, performances
Subdermal Silicone and Collagen Injection Effect on the Skin of Rats (Rattus norvegicus) on the Third Day After Injection Janice Viary
Jurnal Sain Veteriner Vol 40, No 1 (2022): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.62198

Abstract

Collagen and silicone are substances commonly used as dermal filler in a number of beauty procedures. However, sometimes these chemicals cause unwanted side effects on the skin. The purpose of this research is to analyse the effect of collagen and silicone subdermal injection on rat skin as a model for human skin. Materials in this research are 9 Wistar rats (Rattus norvegicus) divided into three groups with a number of 3 rats in each group. The first control group (K) is not given any injections, while KI and KII are injected with 0,1 ml collagen and 0,1 ml silicone respectively. On the third day, all groups are observed for macroscopic changes. Skin samples are taken by necropsy and are made into slides using HE stain. All procedures done are approved by the ethics commission of LLPT, UGM, Yogyakarta with the certificate number 00024/04/LPPT/V/2019. The slides are observed under a microscope with 20x and 40x magnification. Any microscopic changes are noted, also epidermis and dermis lengths are measured. Mild hyperkeratosis, inflammation and proliferation of connective tissue are found. The measurements of the  epidermis and dermis are analysed using T-test independent method in the program Statistical Product and Service Solution (SPSS). The mean length of the epidermis is a little thicker than normal but is not significant (P>0,05). The results of this research show that the subdermal injection of 0,1 ml collagen and 0,1 ml silicone on rats do not cause significant macroscopic or microscopic changes. 
Gambaran Histopatologi Hepatopankreas Kepiting Bakau (Scylla serrata) di Kawasan Estuari Alue Naga, Banda Aceh Indonesia Dedi Fazriansyah Putra; Nurul Fajar; Cut Dahlia Iskandar
Jurnal Sain Veteriner Vol 40, No 1 (2022): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.62805

Abstract

The study aimed to investigate the histopathology status of the hepatopancreas of mangrove crabs (Scylla serrata) in the Alue Naga estuary area, Banda Aceh, Indonesia. The research was conducted in September and October 2020. The purposive sampling method was used where the determination of the location was based on water conditions and anthropogenic activities near the estuary. The mangrove crabs (Scylla serrata) sampling location was implemented within three sampling sites in the Krueng Cut estuary, Gampong Alue Naga, Syiah Kuala District, Banda Aceh. Histopathological analysis was carried out in the histology laboratory, Syiah Kuala University. The results showed that there were changes in the hepatopancreas in the form of irregular lumen star shape, the formation of vacuoles and basophilic hypertrophy. These hepatopancreas changes were assumed due to water pollution generated by anthropogenic activities from local settlements and household industries. Therefore, it is suggested to conduct further research regarding water quality parameters and heavy metal concentration within this sampling area.

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