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Jurnal Kedokteran Hewan

JKH
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Published by Universitas Syiah Kuala

ISSN : 1978225X EISSN : 25025600 DOI : 10.21157

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian Veterinary Medical Association (PDHI). Jurnal Kedokteran Hewan is a double-blind review process journal that has been accredited by National Journal Accreditation (ARJUNA), with second grade (Sinta 2), Number: 200 / M / KPTS / 2020. This journal has been registered in the Indonesian Publication Index (IPI), Google Scholar, Sinta, World Cat, Directory of Open Access Journals (DOAJ), EBSCO, Copernicus, Microsoft Academic, and other scientific databases. Jurnal Kedokteran Hewan receives scientific manuscripts in veterinary sciences (veterinary miscellaneous): anatomy, histology, physiology, pharmacology, parasitology, microbiology, epidemiology, veterinary public health, pathology, reproduction, clinic veterinary, aquatic animal disease, animal science, and biotechnology.

Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)

Articles 901 Documents

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CHARACTERISTIC OF OVARIAN AND ESTRADIOL CONCENTRATIONS IN THE FOLLICULAR FLUID OF SLAUGHTERED ACEH CATTLE Mulyadi Adam; Gholib Gholib; Hafizuddin Hafizuddin; Rumi Sahara Zamzami; Muhammad Bahi
Jurnal Kedokteran Hewan Vol 13, No 4 (2019): December
Publisher : Universitas Syiah Kuala

The objective of this study was to characterize the morphology of ovary from slaughtered Aceh cow and measuring the concentration of estradiol in the follicular fluid to examine the potential utilizing for in-vitro fertilization (IVF) development in Aceh cattle. In total, 30 pairs of ovaries were collected from 30 Aceh cattle slaughtered at Banda Aceh abattoir. Morphological characteristics of ovaries observed were the size of ovaries, weight of ovaries, number of follicles, the diameter of follicles, corpus luteal (CL), and volume of follicular fluid. The follicular fluid was aspirated using a disposable syringe and analyzed the estradiol concentrations using enzyme-linked immunosorbent assay (ELISA). The follicles were grouped into 3 categories based on the diameter (Ø) of follicles: small follicles (Ø 1 - 5 mm), medium follicles (Ø 5 - 8 mm), and large follicles (Ø 8 mm). The morphological characteristic of ovaries varied between right and left ovaries in which right ovaries showed bigger size and weight compared to the left ovaries (P0.05), whereas the number of follicles and follicular fluid did not significantly different (P0.05). The concentrations of estradiol in follicular fluid of large and medium follicles were significantly higher compared to small follicles (P0.05), whereas the concentration of estradiol between large and medium follicles, did not significant different (P0.05). In conclusion, the follicles from abattoir can be used as potential oocytes resources to support IVF development for Aceh cattle in the future. The follicular fluid provided from these follicles can be an alternative for in vitro maturation media of oocytes due to containing high estradiol concentrations.

CORRELATION BETWEEN PROGESTERONE HORMONE CONCENTRATION AND NUMBER OF FETUSES OF ETAWA CROSSBRED GOAT IN DIFFERENT GESTATION PERIOD Syafruddin Syafruddin; Khairul Rizal; Rosmaidar Rosmaidar; Juli Melia; roslizawaty roslizawaty; Zuhrawati Zuhrawati; Erdiansyah Rahmi; Mahdi Abrar
Jurnal Kedokteran Hewan Vol 11, No 3 (2017): September
Publisher : Universitas Syiah Kuala

This study aimed to investigate the correlation between progesterone hormone concentration and number of fetus as well as the difference in progesterone hormone concentration from different gestation periods in Etawah crossbred (EC). Five EC goats used in this study were injected with 2 mL of 5.5 mg prostaglandin F2α (PGF2α, Capriglandin) intramuscularly, with an interval of 10 days. Goats that showed symptoms of estrus were mated naturally with male goats. Blood samples were taken on the 7th, 14th, 21st, and 75th days of gestation. Progesterone hormone concentration was determined using enzyme linked immunosorbent assay (ELISA). The number of fetus was determined at 35th gestational day using ultrasonography (USG). Four out of 5 goats were found to have 1 fetus and 1 goat had 2 fetuses. The progesterone concentration on the 7th, 14th, 21st, and 75th days of gestation in goats with single fetus were 5.21, 7.78, 11.97, and 18.78 ng/mL, respectively, while in goat with two fetuses were 8.44, 14.53, 16.81, and 22.73 ng/mL, respectively. The correlation (r) between progesterone hormone concentration and number of fetus on the 7th, 14th, 21st, and 75th days of gestation were 0.442, 0.854, 0.592, and 0.757, respectively. It is concluded that progesterone concentrations are highly correlated to the number of fetus in each gestation period in EC goats.

ENDOPARASITES INFECTION IN BLOOD COCKLE (Anadara granosa) IN ACEH BESAR WATERS, INDONESIA Dedi Fazriansyah Putra; Suci Ramadina; Siska Mellisa; Muhammadar Abdullah Abbas; Xiao He he
Jurnal Kedokteran Hewan Vol 15, No 3 (2021): September
Publisher : Universitas Syiah Kuala

This study aims to investigate the type, prevalence, and intensity of endoparasites infecting the organs of blood cockles (Anadara granosa) in the waters of Aceh Besar, Indonesia. A purposive sampling method was conducted from March to August 2020 consisting of two sampling sites, namely the waters of Ujung Pancu and Krueng Raya. Endoparasite identification and analysis was carried out at the Parasite Laboratory of the Fish Quarantine Center, Quality Control and Safety of Fishery Products in Blang Bintang, Aceh Besar. The results showed that there were two types of endoparasites living in the blood cockles: namely, Perkinsus sp., which belong to the group Protozoa, and Spiroxys sp. of the Nematode class. The prevalence levels of Perkinsus sp. was 73.33% and were found in the waters of Ujung Pancu; while the lowest prevalence level was 20% in blood cockles that were infected with Spiroxys sp., which were found both in Ujung Pancu and Krueng Raya. The highest intensity level was infections of Perkinsus sp. with a value of 89 ind/species in the Krueng Raya site. The lowest intensity value was infections of Spiroxys with a value of 2 ind/species in Krueng Raya and 2.3 ind/species in Ujung Pancu

MORPHOLOGY AND MORPHOMETRY OF Haemonchus contortus IN GOATS IN YOGYAKARTA, INDONESIA Priyo Sambodo; Joko Prastowo; Sudarmanto Indarjulianto; Kurniasih Kurniasih
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

This research was carried out to determine the morphology and morphometry of Haemonchus contortus in goats. Adult female parasites were collected from abomasum of naturally infected goats and stored in lactophenol. Images of the parasite were acquired using lucida camera and measurement was performed using Axiovision LE software. Basic characters of the morphology and morphometry of Haemonchus spp. were identified by determining the value of the discriminant function of three parameters on spicule. Vulvar morphology was identified by vulvar flap shape. Data were presented in terms of mean, standard deviation, and percentage. A hundred percent of the parasites were H. contortus with discriminant function value 0.63. The size of female parasites was longer and wider (25.5-32.6 mm; 0.38-0.63 mm) than that of male ones (17.3-20.0 mm; 0.24-0.33 mm). There were only 2 varieties of vulvar flap, linguiform (81%), and knobbed (19%). There were 5 subclasses identified in the linguiform type, including linguiform A (39%), linguiform B (22%), linguiform C (17%), linguiform I (1%), and a new subclass of linguiform D (2%). Based on the the type of vulvar flap, H. contortus found in goats in Yogyakarta were different to those found in previous researches. The findings could be used in identifying the parasite species in small ruminants.

EKSPRESI INSULIN PADA PANKREAS MENCIT (Mus musculus) YANG DIINDUKSI DENGAN STREPTOZOTOCIN BERULANG Erwin E; Etriwati E; Muttaqien M; Tri Wahyu Pangestiningsih; Sitarina Widyarini
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui ekspresi insulin pada pankreas mencit (Mus musculus) yang diinduksi streptozotocin berulang dengan pewarnaan imunohistokimia yang berguna sebagai hewan model diabetes melitus. Tiga puluh ekor mencit jantan galur Balb-C, umur 12-14 minggu dengan bobot badan 30-40 g dikelompokkan menjadi 2 kelompok perlakuan, masing-masing kelompok terdiri atas 15 ekor. Kelompok 1 (K1) diberikan pelarut streptozotocin, sedangkan kelompok 2 (K2) diberikan streptozotocin dengan dosis 40 mg/kg bobot badan dalam 50 mM natrium sitrat bufer pH 4,5 secara intraperitoneal sebanyak 0,5 ml selama 5 hari berturut-turut. Hewan percobaan dari masing-masing kelompok dieutanasia sebanyak 2 ekor pada hari ke-0, 7, 14, 21, dan 28 setelah perlakuan, selanjutnya mencit diperfusi dan dinekropsi untuk mengambil jaringan pankreas sebagai sampel pemeriksaan imunohistokimia dengan metode streptavidin peroksidase menggunakan antibodi mouse anti-insulin (1:300). Berdasarkan uji statistik menggunakan analisis varian, ekspresi insulin pada sel beta Langerhans pankreas K1 lebih tinggi dibandingkan K2 (P0,05). Waktu pengamatan dan interaksi antara kelompok dan waktu pengamatan menunjukkan perbedaan yang signifikan (P0,05). Induksi dosis rendah streptozotocin secara berulang dapat menurunkan jumlah ekspresi sel beta Langerhans pankreas yang imunoreaktif terhadap insulin.

KUALITAS SPERMATOZOA AYAM PERANAKAN SENTUL DALAM PENGENCER RINGER LAKTAT KUNING TELUR DENGAN BERBAGAI MONOSAKARIDA (Quality of Sentul Crossbreed Chicken Spermatozoa in Ringer Lactate-Egg Yolk Diluents Supplemented with Various Monosaccharide) Khaeruddin Khaeruddin; Raden Iis Arifiantini; Cece Sumantri; Sri Darwati
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

The aim of this study was to examine the preservation of sentul crossbreed chicken semen in ringer lactate egg yolk diluent supplemented with various monosaccharide. Semen was collected from three roosters using massage method. Immediately after collection, the semen was evaluated macroscopically and microscopically. Semen with more than 70% motility was divided into four tubes. Each of them diluted with ringer lactate egg yolk glucose (RLEYG), ringer lactate egg yolk fructose (RLEYF), ringer lactate egg yolk xylose (RLEYX) and ringer lactate egg yolk mannose (RLEYM). Semen was stored in refrigerator (5o C) for sixty hours and evaluated every twelve hours for spermatozoa motility and viability. Results showed that no significant difference (P0.05) among diluents used on spermatozoa quality parameters after dilution and during preservation. Semen quality decrease during storage and at sixty hours of storage, the motility and viability of spermatozoa ranging from 48.33±2.56 to 55.42±2.26% and 58.59±2.87 to 64.83±2.42%, respectively. This research conclude that glucose, fructose, xylose and mannose can be used as energy source for roosters semen during preservation.

KESEMBUHAN LUKA SETELAH PEMBERIAN TOPIKAL ZINK PADA TIKUS DENGAN PAKAN LEMAK TINGGI Devita Anggraeni; Dhirgo Adji; Retno Murwanti
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui peran zink pada kesembuhan luka dalam kondisi berat badan berlebih. Dalam penelitian digunakan 20 ekor tikus Sprague Dawley jantan berumur tiga bulan (150-200 g). Tikus dibagi secara acak menjadi empat kelompok (A, B, C, dan D), masing-masing kelompok terdiri atas lima ekor. Kelompok A dan B diberi pakan normal, sedangkan kelompok C dan D diberi pakan lemak tinggi. Setelah dua bulan perlakuan pakan, seluruh tikus dioperasi untuk membuat luka irisan pada kulit, kemudian luka ditutup dengan jahitan. Luka jahitan pada kelompok A dan C diberi aplikasi topikal unguenta vaseline album (tanpa zink), sedangkan luka jahitan pada kelompok B dan D diberi aplikasi topikal unguenta zink 10% dengan bahan dasar vaselin album. Lima hari setelah operasi, seluruh tikus diambil darahnya untuk dianalisis terhadap jumlah leukosit total kemudian diikuti pengambilan jaringan kulit untuk pemeriksaan histopatologis dan penghitungan jumlah leukosit di jaringan. Hasil penelitian menunjukkan adanya interaksi yang signifikan antara pakan dan aplikasi topikal terhadap jumlah leukosittotal dalam darah (P0,05). Jumlah leukosit di jaringan mengalami penurunan setelah aplikasi topikal zink, meskipun hasilnya tidak signifikan (P0,05). Analisis histopatologis menunjukkan epidermis sudah tertutup pada seluruh tikus perlakuan, namun demikian sebagian tikus masih menunjukkan dermis yang terbuka, yaitu kelompok A sebanyak 60%, kelompok B sebanyak 60%, kelompok C sebanyak 60%, dan kelompok D sebanyak 40%. Dapat disimpulkan bahwa proses kesembuhan luka setelah pemberian topikal zink pada tikus yang diberi pakan lemak tinggi menunjukkan hasil yang baik, yaitu ditandai dengan penutupan lapisan epidermis dan dermis, serta menurunnya jumlah leukosit di jaringan.

Antimicrobial Activity and LC Determination of Ethanol Crude Extract of 50 Pliek U, an Achehnese Traditional Fermented Food Nurliana Nurliana; Mirnawati Sudarwanto; Lisdar I. Sudirman; Angelina W. Sanjaya
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): March
Publisher : Universitas Syiah Kuala

Antimicrobial activity of pliek u crude extracts was evaluated against five bacterial species (Staphylococcus aureus, Bacillus cereus, Escherichia coli, Salmonella enterica serovar Enteritidis and Pseudomonas aeruginosa,)and one fungal species (Candida albicans). Pliek u has been consumed as spices and hot sauce and poultry feed. These foods were collected from household industry at Reudep village in Aceh Besar, Province of Aceh. The ethanol extract of pliek u were obtained by standard method. The antimicrobial activity was detected using paper disc method. The concentration of ethanol crude extract of pliek u (EEP) was determined with the dilution method. The lethality initial test has been detected by using Artemia salina L. bioassay to determine the toxic concentration based on the LC value of EEP. The ethanol crude extract (EEP) was active against all microbial strains. EEP showed 50 antimicrobial activity at a minimum inhibitory concentration (MIC) and a minimum microbicidal concentration (MMC) at 2.5-10 mg/ml and 10-20 mg/ml, respectively. The lethality concentration EEP gave the LC value of 3.36 50 mg/ml. It was concluded that ethanol crude extract (EEP) shows significant antimicrobial activity and it is not toxic.

SINKRONISASI ESTRUS DAN PENGAMATAN ULTRASONOGRAFI PEMERIKSAAN KEBUNTINGAN DINI PADA DOMBA GARUT (Ovis aries) SEBAGAI STANDAR PENENTUAN UMUR KEBUNTINGAN Amrozi a; Bagus Setiawan
Jurnal Kedokteran Hewan Vol 5, No 2 (2011): September
Publisher : Universitas Syiah Kuala

Penelitian ini dilakukan untuk mengamati sinkronisasi estrus dan menentukan diagnosis kebuntingan dini pada domba garut (n=3) menggunakan ultrasonografi transrektal. Domba disinkronisasi dengan menggunakan PGF pada fase luteal. Onset dan durasi estrus diamati dengan menggunakan jantan pengusik. Kebuntingan 2a ditentukan dengan tampilan isoechogenic yang dikelilingi oleh tampilan hypoechogenic. Rata-rata onset estrus adalah 35±28,7 jam dan rata-rata lama estrus adalah 33±13,6 jam. Kebuntingan dini terdeteksi pada hari ke-22 (22,3±0,6 hari). Perkembangan fetus diikuti dengan peningkatan diameter dan ketebalan uterus. Diameter uterus meningkat dari hari ke-22 (1,8±0,7 cm) hingga hari ke-42 (5,6±1,1 cm), dan tebal uterus meningkat dari hari ke-22 (0,8±0,1 cm) hingga hari ke-42 (2,1±0,5 cm). Plasentom muncul pada kebuntingan hari ke-34 (0,8±0,2 cm) dan menunjukkan pola perkembangan yang terus meningkat secara signifikan sampai hari ke-56 (2,7±0,5 cm; P0,05). Diameter kotiledon hari ke-34 sekitar 0,8±0,2 cm hingga hari ke-56 (2,7±0,5 cm) dan hari ke-77 (3,3±0,4 cm). Dapat disimpulkan bahwa diagnosis positif dari kebuntingan pada domba garut terlihat pada hari ke-12 dan fetus dapat diamati pada hari ke-22.

POTENSI TRANSDIFERENSIASI SEL FIBROBLAS MENJADI SEL SARAF SECARA IN VITRO (Transdifferentiation Potency of Fibroblast Cell to Neuron Cell in Vitro) Ekayanti M. Kaiin; Ita Djuwita
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

The aim of this study was to examine the potency of fibroblast cells transdifferentiated to neuron cells in vitro. Newborn rat neuron conditioned medium (NBRN CM) was collected from neuron cells cultured with mDMEM without serum for 48 hours. Fibroblast cells were collected from fetal rat muscle treated with trypsin. Fibroblast cells were culture with 3 kind of culture medium: mDMEM + 0.01 mM β-mercaptoethanol; mDMEM + 50% NBRN-CM and mDMEM + 0.01 mM β-mercaptoethanol + 50% NBRN CM . As control, cells was cultured with mDMEM +10% newborn calf serum (NBCS). The addition of NBRN CM into culture medium resulted in 12.97% newborn cells in fibroblast culture medium passage I. Newborn rat neuron conditioned medium in fibroblast culture medium resulted 12.97% neuron cells at passage 1. The percentage was increased (14.60%) when β- mercaptoethanol added into medium. The same result was found at passage 3 (12.67%; 13.17%). It showed that fibroblast cells has potency to transdifferentiated into neuron cells when cultured with NBRN CM. Further research is needed to know the fibroblast transdifferentiation potency. Key words: fibroblast, transdifferentiation, conditioned medium, neuron cells, in vitro

Page 35 of 91 | Total Record : 901

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Tongku Nizwan Siregar

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Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

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Aceh

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Contact Name Tongku Nizwan Siregar

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Editorial Address Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

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Abstract

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Contact Name
Tongku Nizwan Siregar

Contact Email
jkh@usk.ac.id

Phone
+626517551536

Journal Mail Official
jkh@usk.ac.id

Editorial Address
Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location
Kab. aceh besar,

Aceh

INDONESIA