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Jurnal Kedokteran Hewan

JKH
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Published by Universitas Syiah Kuala

ISSN : 1978225X EISSN : 25025600 DOI : 10.21157

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian Veterinary Medical Association (PDHI). Jurnal Kedokteran Hewan is a double-blind review process journal that has been accredited by National Journal Accreditation (ARJUNA), with second grade (Sinta 2), Number: 200 / M / KPTS / 2020. This journal has been registered in the Indonesian Publication Index (IPI), Google Scholar, Sinta, World Cat, Directory of Open Access Journals (DOAJ), EBSCO, Copernicus, Microsoft Academic, and other scientific databases. Jurnal Kedokteran Hewan receives scientific manuscripts in veterinary sciences (veterinary miscellaneous): anatomy, histology, physiology, pharmacology, parasitology, microbiology, epidemiology, veterinary public health, pathology, reproduction, clinic veterinary, aquatic animal disease, animal science, and biotechnology.

Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)

Articles 901 Documents

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PENGGANTIAN BOVINE SERUM ALBUMIN PADA PENGENCER CEP-2 DENGAN SERUM DARAH SAPI DAN PUTIH TELUR TERHADAP KUALITAS SEMEN CAIR SAPI LIMOUSIN SELAMA PENDINGINAN (The Substitution of Bovine Serum Albumin with Cattle Blood Serum and Egg White in CEP-2 Diluent on the Quality of Limousin Bull Liquid Semen during Refrigerating) Trinil Susilawati; Feri Eka Wahyudi; Inna Anggraeni; Nurul Isnaini; Muhammad Nur Ihsan
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

This study aims to determine the effect of the substitution of bovine serum albumin (BSA) with cattle blood serum and egg white in the diluent of Cauda epididymal Plasma 2 (CEP-2) on sperm quality of Limousin cattle during cooling at 3-5 C. The research material used was rejected Limousin bull sperm (motility of 50-60%) from Artificial Insemination Centre Singosari, Malang. This research was a laboratory experiment using a randomized block design which was composed of six treatments with 10 replications, those were T0 as controls ((90% CEP-2 with BSA + 10% egg yolk); T1 (83.84% CEP-2 + 6.16% cattle blood serum + 10% egg yolk); T2 (81.84% CEP-2 + 8.16% cattle blood serum + 10% egg yolk); dan T3 (90% CEP-2 + 0,4% egg white + 10% egg yolk); T4 (90% CEP-2 + 0.8% egg white + 10% egg yolk); and T5 (90% CEP-2 without BSA + 10% egg yolk). Parameters measured were the percentage of motility, viability, and abnormality of sperms. Results of research after 48 hours of storage showed that the percentage of sperm motility in T0, T1, T2, T3, T4, and T5 were 40.50±5.90, 36±36.16, 34.00±6.58, 40.50±3.69, 38.50±3.37, and 38.50±4.12, respectively, while the percentage of sperms viability were 75.16±4.30, 70.50±2.88, 73.80±2.80, 74.80±3.30, 75.13±3.13, and 74.03±4.13, respectively, and the percentage of sperms abnormality were 10.14±2.34, 10.62±1.34, 11.33±2.00, 10.94±2.82, 10.02±1.95, and 10.78±1.96, respectively. In conclusion, CEP-2 diluent with or without the addition of 19% egg yolk in BSA and the substitution of BSA with 0.4-0.8% egg white can maintain semen quality to hour of 48 in cold storage.This study aims to determine the effect of the substitution of bovine serum albumin (BSA) with cattle blood serum and egg white in the diluent of Cauda epididymal Plasma 2 (CEP-2) on sperm quality of Limousin cattle during cooling at 3-5 C. The research material used was rejected Limousin bull sperm (motility of 50-60%) from Artificial Insemination Centre Singosari, Malang. This research was a laboratory experiment using a randomized block design which was composed of six treatments with 10 replications, those were T0 as controls ((90% CEP-2 with BSA + 10% egg yolk); T1 (83.84% CEP-2 + 6.16% cattle blood serum + 10% egg yolk); T2 (81.84% CEP-2 + 8.16% cattle blood serum + 10% egg yolk); dan T3 (90% CEP-2 + 0,4% egg white + 10% egg yolk); T4 (90% CEP-2 + 0.8% egg white + 10% egg yolk); and T5 (90% CEP-2 without BSA + 10% egg yolk). Parameters measured were the percentage of motility, viability, and abnormality of sperms. Results of research after 48 hours of storage showed that the percentage of sperm motility in T0, T1, T2, T3, T4, and T5 were 40.50±5.90, 36±36.16, 34.00±6.58, 40.50±3.69, 38.50±3.37, and 38.50±4.12, respectively, while the percentage of sperms viability were 75.16±4.30, 70.50±2.88, 73.80±2.80, 74.80±3.30, 75.13±3.13, and 74.03±4.13, respectively, and the percentage of sperms abnormality were 10.14±2.34, 10.62±1.34, 11.33±2.00, 10.94±2.82, 10.02±1.95, and 10.78±1.96, respectively. In conclusion, CEP-2 diluent with or without the addition of 19% egg yolk in BSA and the substitution of BSA with 0.4-0.8% egg white can maintain semen quality to hour of 48 in cold storage.

ISOLASI DAN KARAKTERISASI BIOLOGIK VIRUS NEWCASTLE DISEASE Emilia A; Surachmi Setiyaningsih; Retno Damayanti Soejoedono
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Tujuan dari penelitian ini adalah memperoleh informasi infeksi dan sifat-sifat biologis virus Newcastle disease (ND) pada ayam ras maupunayam buras. Sejumlah 529 sampel usap kloaka dikoleksi dari pasar-pasar tradisional dan peternakan rakyat di daerah Bogor dan Tangerang. Sebanyak 20 sampel terdeteksi positif melalui real time reverse transcription-polymerase chain reaction (RRT-PCR) sedangkan dengan isolasi virus terdeteksi positif sebanyak 11 sampel. Selanjutnya empat isolat representasi lokasi dipilih untuk karakterisasi patogenisitas dengan metode mean death time (MDT) pada telur ayam berembrio (TAB) specific pathogen free (SPF), dan karakterisasi antigenisitas menggunakan metode haemagglutination inhibition (HI) dan virus netralisasi (VN) untuk melihat keragaman antar isolat virus. Uji MDT memperlihatkan dua isolat(kode isolat II dan XIII) adalah mesogenik, satu isolat (kode isolate VIII) termasuk lentogenik, dan satu isolat (kode isolat TW) bersifat velogenic. Tiga dari empat isolat menunjukkan hubungan antigenik dengan virus ND strain Komarov dan G7 (genotipe 7) tetapi tidak dengan strain referensi Lasota, sementara isolat velogenic (kode TW) yang berasal dari ayam kampung menunjukkan reaksi silang tinggi dengan antiserum terhadap tiga strain referensi di atas. Sampel-sampel yang diambil di lapang baik itu berasal dari pasar dan peternakan rakyat meskipun secara klinis tidak memperlihatkan gejala sakit dapat diisolasi virus ND dengan karakteristik yang beragam.

THE REPRODUCTIVE CHARACTERISTICS OF GAYO BUFFALOES AS A LOCAL LIVESTOCK GENETIC RESOURCE IN THE MESIDAH SUBDISTRICT, BENER MERIAH REGENCY Mohd. Agus Nashri Abdullah; Tudung Mulie Gundala; Eka Meutia Sari
Jurnal Kedokteran Hewan Vol 16, No 4 (2022): December
Publisher : Universitas Syiah Kuala

This research aimed to identify the reproductive characteristics of Gayo buffaloes in the Mesidah Sub-district, Bener Meriah Regency. There were three locations of the current study namely Perumpaken Benjadi, Wer Tingkem, and Simpur, which were determined using the purposivesampling technique. A survey was used as the research method to obtain primary data and secondary data. Primary data were collected throughdirect interviews with buffalo livestock owners. They were asked a set of questions that had been prepared in a questionnaire. Secondary datawere obtained from related agencies in Bener Meriah Regency. The number of respondents was 92 from four villages in the Mesidah Sub-district,Bener Meriah Regency. Breeders as respondents were selected using a purposive sampling method, with minimum requirements that they hadraised two buffaloes that had given birth more than once and that the breeders had at least four years of farming experience. The research datawere analyzed using descriptive statistic. The results showed that the Gayo buffaloes in the Mesidah Sub-district, Bener Meriah Regency, had reproductive characteristics that met the standards. This can be seen from the obtained data which were almost the same as the average age of the reproductive characteristics of buffaloes in Indonesia. Gayo buffaloes in the Mesidah Sub-district experienced the first estrus (puberty) at 33.83 months old, first mating at 34.61 months old, and first calving at 45.29 months. The average gestation period was 10.51 months, the calving interval was 15.5 months, and the average service period was 3.66 months, while maintenance management was carried out extensively. Based on the results, it can be concluded that the reproductive characteristics of Gayo buffaloes in the Mesidah Sub-district, Bener Meriah Regency, still meet the standards. The average reproductive characteristics of Gayo buffaloes are generally the same as the average characteristics of other buffaloes in Indonesia.

Cytoskeleton of Bovine Oocytes after Vitrification Using Ethylene Glycol Cryoprotectant Sri Wahjuningsih
Jurnal Kedokteran Hewan Vol 2, No 2 (2008): September
Publisher : Universitas Syiah Kuala

The objective of this research is to know the influence of vitrification method toward thecondition of bovine oocytes cytoskeleton based on microtubules using the immunohistochemistrytechnique. Oocytes classified in two treatments such as fresh oocytes (control) and oocytes withvitrification treatments of 10, 20, 30, 40, and 50% of ethylene glycol (EG). Data were analyzed bydescriptive analyzing. The results of this research indicated that vitrification treatments were affected tomicrotubules organization of oocytes. Concentration of EG 10, 20, 40, and 50% of EG could not repaircytoskeleton after vitrification, but cytoskeleton structure could be protected by EG 30%.

DIAGNOSIS CEPAT VIRUS AVIAN INFLUENZA TIPE A SUBTIPE H5 DARI SPESIMEN LAPANGAN DENGAN METODE ONESTEP SIMPLEX RT-PCR Aris Haryanto; Duhita Andinita; Sri Handayani Irianingsih; Dini Wahyu Yudianingtyas
Jurnal Kedokteran Hewan Vol 6, No 1 (2012): March
Publisher : Universitas Syiah Kuala

Virus avian influenza (AI) merupakan virus dengan materi genetik RNA single-stranded sense negatif, beramplop yang termasuk dalam famili Orthomyxoviridae. Onestep simplex reverse transcriptase-polymerase chain reaction (RT-PCR) merupakan salah satu metode diagnosis yang dapat diandalkan untuk mendeteksi virus AI. Tujuan dari penelitian ini adalah untuk menerapkan metode diagnosis cepat virus AI pada spesimen lapangan secara langsung dari pasar unggas berdasarkan amplifikasi RT-PCR gen M dan H5 dengan metode yang berbasis onestep simplex RT-PCR tanpa melalui proses inokulasi dan propagasi virus AI dalam telur ayam berembrio (TAB). Sebanyak 35 sampel spesimen lapangan dari swab trakea unggas yang berasal dari pasar unggas di Terban, Kotamadya Yogyakarta digunakan dalam penelitian ini. Amplifikasi DNA secara onestep simplex RT-PCR pada gen matriks (M) dilakukan terhadap seluruh sampel. Pada sampel-sampel yang menunjukkan hasil positif pada amplifikasi gen M kemudian dilakukan amplifikasi RT-PCR secara lebih lanjut untuk gen H5 virus AI. Produk hasil amplifikasi RT-PCR gen M dan H5 divisualisasikan menggunakan elektroforesis gel agarose konsentrasi 1% dengan pewarnaan SYBR® Safe DNA Gel Staining. Hasil amplifikasi RT-PCR gen M menunjukkan bahwa dari 35 sampel diperoleh 8 sampel positif terinfeksi virus AI tipe A yang ditunjukkan dengan adanya fragmen DNA sebesar 200 bp, sedangkan hasil amplifikasi gen H5 sebanyak 5 dari 8 sampel-sampel tersebut merupakan virus AI tipe A subtipe H5 yang ditunjukkan dengan adanya fragmen DNA sebesar 545 bp. Diagnosis cepat virus AI tipe A subtipe H5 secara langsung dari spesimen lapangan di pasar unggas dapat dilakukan dengan metode onestep simplex RT-PCR, namun metode diagnosis tersebut tidak dapat mendeteksi keberadaan virus AI dalam sampel yang virusnya terlalu sedikit.

The Ectoparasites Inventory on Cultured Carp in Floating Cage Net at Laut Tawar Lake Kabupaten Aceh Tengah Winaruddin W; Eliawardani E
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): September
Publisher : Universitas Syiah Kuala

The aimed of this research is inventoried the parasites of carp in floating cage net at Laut TawarLake. This study used 100 fishes consisted of 50 fries and 50 reproductive stadia. The result of thisresearch indicates the parasites found in the fishes are Trichodina sp., Ichtyophthirius multifiliis,Dactylogyrus sp., Gyrodactylus sp., Epistylis sp. and Lernaea sp. Protozoan parasites were found in allfishes and infected fish organs, while metazoan parasites infected fins and gills and copepods only infectedskin.Keywords: parasites, carps, floating cage net, Laut Tawar Lake

KAJIAN DIVERSITI GENETIKA Tarsius sp. ASAL INDONESIA MENURUT URUTAN GEN NADH DEHIDROGENASE SUBUNIT 4 (ND4) Herrialfian H; Rini Widayanti; Hery Wijayanto; Jalaluddin J
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengkaji keragaman genetik gen penyandi ND4 pada Tarsius bancanus, T. b. borneanus, T. dianae dan T. spectrum dan untuk penegakan taksonominya. Deoxyribonucleic acid (DNA) diisolasi dari biopsi jaringan masing-masing spesies Tarsius dengan cara diekstraksi untuk digunakan sebagai DNA cetakan dalam proses amplifikasi dengan metode polymerase chain reaction (PCR). Primer yang digunakan dalam penelitian ini didesain untuk mengamplifikasi gen ND4 dan dilanjutkan dengan elektroforesis. Produk PCR hasil amplifikasi yang telah dimurnikan, selanjutnya dipergunakan sebagai DNA cetakan untuk reaksi penentuan runutan nukleotida. Runutan nukleotida gen ND4 hasil pengurutan dilakukan penjajaran berganda dengan primata lain yang diambil dari Genbank menggunakan Clustal W. Selain berdasarkan runutan nukleotida, gen ND4 dianalisis berdasarkan runutan asam amino dari basa-basa yang diterjemahkan mengikuti vertebrate mitochondrial translation code yang ada pada program MEGA versi 4.1. Konstruksi pohon filogenetika menggunakan metode neighbor joining. Hasil penelitian menunjukkan dari 1378 nukleotida ditemukan 119 situs yang bersifat beragam. Jarak genetika berdasarkan nukleotida gen ND4 yang dihitung menggunakan model dua parameter Kimura, terdapat nilai paling kecil 0,6%, nilai terbesar 13%, dan nilai rata-rata sebesar 6,1%. Filogram berdasarkan hasil runutan nukleotida gen ND4 yang menggunakan metode neighbor joining, dapat mengidentifikasi dan membedakan percabangan antar spesies Tarsius.

ROBUST PREDICTION OF MEAT QUALITY ATTRIBUTES USING NEAR INFRARED SPECTROSCOPY Cut Dahlia Iskandar; Z Zainuddin; Agus Arip Munawar; Rina Aulia Barus
Jurnal Kedokteran Hewan Vol 17, No 1 (2023): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i1.30204

The main purpose of this present study is to evaluate the ability of near infrared technology as an alternative method in determining and assessing quality parameters of meat product where in this case is frozen beef. At first, beef samples from chest and legs parts were sliced and taken at the amount of 100g per sample to be frozen. Then spectra data of beef samples were obtained using near infrared spectrophotometer (PSD IR i16) in wavelength range from 1000 to 2500 nm with optical gain 4x. Actual protein contents were obtained by Kjeldahl method and measured in triplicate. The near infrared spectra data were enhanced and improved by means of mean centering (MC) and baseline shift correction (BSC) methods. The results showed that protein content of frozen beef samples can be predicted rapidly with maximum correlation coefficient is 0.91. Heat properties of beef samples changes exponentially during freezing and thus, optimum freezing temperature and time can be predicted as well. Based on those obtained results, it may conclude that near infrared technology can assess frozen beef qualities rapidly and effectively.

EVALUATION OF B1 GENE TO DETECT Toxoplasma gondii: COMPARISON OF THREE SETS NESTED PCR PRIMER Fitrine Ekawasti; Zul Azmi; Didik Tulus Subekti; muhammad ibrahim desem; Arifin Budiman Nugraha; Siti Sa’diah; Umi Cahyaningsih
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.22251

This study aimed to evaluate three sets of B1 gene DNA primer for the diagnosis of Toxoplasma gondii. The DNA of Toxoplasma gondii that stored on liquid nitrogen was isolated using DNAzol™ reagent. The first step of Polymerase Chain Reaction (PCRs) was performed using external and internal primer sets, respectively, and then nPCR. PCR products sequencing was performed by Apical Science. All sequences were analysed using CLC Sequence Viewer Version 8.0 software and compared to sequence database that deposited in ToxoDB (Toxoplasma gondii genome database) using BLAST (https://toxodb.org/toxo/app). Each B1 gene primer was evaluated by performing single PCR (forward and reverse) and nested PCR reactions. Three sets of B1 gene primer have different amplification precision. According to the results of amplicon sequencing, the primer set #2 has the best amplification precision of B1 gene.

PHYSIOLOGICAL RESPONSES OF FEMALE BEEF CATTLE AGAINST PEATLAND MICROCLIMATE STRESS IN CENTRAL KALIMANTAN Adrial Adrial; Rudy Priyanto; Salundik Salundik; Ahmad Yani; Luki Abdullah
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.29115

This study aimed to evaluate the physiological response of female beef cattle to peatland microclimate stress in Central Kalimantan. This study used direct observation on small holder beef cattle farm. Microclimate data on 41 units cattle barns and physiological parameters of female cattle were collected in the morning, at noon and in the afternoon. The physiological parameter measurements involved 215 female beef cattle, consisting of 119 Bali and 96 crossbred cattle with different physiological stages including pregnant cows, lactating cows, dry cows, heifers and calves. The microclimate condition within cattle barns on peatland of Central Kalimantan is not the comfort zone for beef cattle. It is characterized by high air temperature and relative humidity, and low wind speed, which result in high temperature humidity index (THI). The barn with gable roof type and asbestos materials gave the lowest THI. This Microclimate caused heat stress to beef cattle reared on the peatlands, indicated by the high rectal temperature, heart rate, respiratory rate, and heat tolerance coefficient, although it was still categorized as mild to moderate stress. Bali cattle showed better physiological responses to microclimate stress than crossbred cattle. Pregnant crossbred cows were the most susceptible to peatland microclimate stress.

Page 42 of 91 | Total Record : 901

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Tongku Nizwan Siregar

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Editorial Address Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

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Contact Name
Tongku Nizwan Siregar

Contact Email
jkh@usk.ac.id

Phone
+626517551536

Journal Mail Official
jkh@usk.ac.id

Editorial Address
Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location
Kab. aceh besar,

Aceh

INDONESIA