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Jurnal Media Analis Kesehatan
Published by Politeknik Kesehatan Makassar
ISSN : 20871333     EISSN : 26219557     DOI : https://doi.org/10.32382/jmak
Core Subject : Health, Science,
Health Professions Immunology & microbiology Medicine & Pharmacology
Jurnal Media Analis Kesehatan merupakan Jurnal ilmiah yang memuat tulisan ilmiah yang berkaitan dengan bidang ilmu analis kesehatan atau teknologi laboratorium medik. Jurnal ilmiah yang membahas tentang kompetensi analis kesehatan yang meliputi kompetensi Mikrobiologi, Hematologi, Parasitologi, Imunoserologi, Toxikologi, dan Kimia Klinik
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 41 Documents
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Effect of Bigger Ginger Extract on Reduction of Blood Sugar Rate of Glucose Induced Mice Nurdin Nurdin; Asmawati Asmawati; Sitti Hadijah; Ratnasari Dewi; Widarti Widarti; Muhammad Nasir
Jurnal Media Analis Kesehatan Vol 14 No 2 (2023): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v14i2.241

Abstract

To date, the government and people of Indonesia are still facing health problems. Diabetes, also known as diabetes mellitus, is one of the most common diseases among Indonesians. Some rhizome spices that have antidiabetic potential, one of which is elephant ginger. The purpose of this study was to determine the effectiveness of bigger ginger extract on reducing blood glucose levels in mice. This type of research is an experimental study to determine the effectiveness and concentration of bigger ginger extract on blood glucose levels in mice induced with glucose. Based on the results obtained, Bigger Ginger Rhizome Extract (Zingiber officinale var. Rosc.) has the potential to reduce blood sugar levels in mice with an average reduction value where 5% b/v extract decreased by 48.69%, 10% b/v extract decreased by 50% and 15% b/v extract decreased by 55%. The concentration of 5% b/v in Bigger Ginger Rhizome Extract (Zingiber officinale var. roscoe) shows an optimal reducing effect
Sensitivity and Specificity of Rapid Antigen Antigen Examination for COVID-19 Immunochromatography Method at the Dadi Regional Special Hospitals Herman Herman; Nuradi Nuradi; Herdiansyah Herdiansyah; Herdiana Herdiana; Alfin Resya Virgiawan; M. Askar M. Askar
Jurnal Media Analis Kesehatan Vol 14 No 2 (2023): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v14i2.247

Abstract

Currently COVID-19 is still in pandemic status so the transmission of this disease needs to be controlled. One of the government's efforts is to screen people suspected of COVID-19 with laboratory examinations using RT-PCR, but this is hampered by long test times. For this reason, the Rapid Antigen COVID-19 (RA COVID-19) immunochromatography (IT) method is used which is short in time and uses simple equipment. This research is a laboratory observational research which aims to determine the sensitivity and specificity of the RA COVID-19 method at RSKD Dadi, South Sulawesi Province. The research sample was 50 suspected COVID-19 patients being treated at RSKD Dadi, South Sulawesi Province and then nasopharyngeal swab samples were taken from these patients. The 50 swab samples were examined using the RA COVID-19 method and the Real Time Polymerase Chain Reaction (RT-PCR) method. Location The research was carried out at the RSKD Dani laboratory, South Sulawesi Province for 6 months (July-December 2022). The research results showed that the RA COVID-19 used at RSKD Dadi, South Sulawesi Province as a COVID-19 screening method for suspected COVID-19 patients had a sensitivity of 87% and a specificity of 97%. Conclusion: RA COVID-19 used at RSKD Dadi, South Sulawesi Province can be used as a screening method, but the results still need to be confirmed using the RT-PCR method
Potential of Cassava Peel as an Alternative Growth Media of Aspergillus niger and Rhizopus oryzae with Concentration Modification Artati Artati; Risky Risky; Rafika Rafika; Zulfian Armah; Syahida Djasang; Asriyani Ridwan; Aan Yulianingsih Anwar
Jurnal Media Analis Kesehatan Vol 14 No 2 (2023): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v14i2.251

Abstract

Waste is the waste product of a production process or materials that are no longer valuable or worthless, including waste from food processing processes. One of the main food wastes produced in developing countries is cassava peel from a plant with the Latin name Manihot ultisima. Cassava skin is the outer layer of the tuber which functions as a protector for the cassava flesh. This research aims to prove that cassava peel can be used as raw material for making alternative media for fungal growth and to find out the most effective concentration to use. This research is experimental using cassava skin as a sample. In this research, data analysis was carried out using the One-Way Anova Test. Concentrations of 5%, 10%, 15%, 20%, 25% and 30% were made as alternative media for the growth of Aspergillus niger and Rhizopus oryzae fungi and SDA media as a control. During the incubation period for these two species, both Aspergillus niger and Rhizopus oryzae experienced growth which became bigger day by day. The conclusion of this research is that cassava peel can be used as a natural ingredient that can be used as an alternative medium for fungal growth. In the alternative media for cassava peel, the most effective concentration of the Aspergillus niger species is 20%, while for the Rhizopus oryzae species it is 15%
Modification of the Calculation of the Examination of the Platelet Count Using 1 Large Box of Leukocytes in the Improved Neubauer Counting Room Zulfikar Ali Hasan; Nurdin Nurdin; Ridho Pratama; Artati Artati; Yaumil Fachni Tandjungbulu; Andi Fajrin Permana
Jurnal Media Analis Kesehatan Vol 14 No 2 (2023): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v14i2.256

Abstract

The direct examination of the platelet count using the improved Neubauer counting chamber is carried out by microscopic examination of 25 Medium Boxes of Erythrocytes, this method requires several field shifts so that more accuracy is needed when carrying out the examination. Modification of the calculation of the platelet count by using 1 large box of leukocytes in the improved Neubauer counting chamber requires only 1 field of view at the time of calculation so that it does not take a long time and is more thorough when carrying out microscopic platelet counts. The purpose of this study was to determine the number of platelets using the calculation of 25 squares while the erythrocytes and the number of platelets using a modification of the calculation of 1 large box of leukocytes. This type of research is a quasi-experimental which aims to determine the effect that arises due to certain treatments for examining the platelet count using 25 boxes while the erythrocytes and modification of the calculation using 1 large box of leukocytes. It was carried out at the Hematology Laboratory, Department of Medical Laboratory Technology, Poltekkes Kemenkes Makassar. This research was carried out to start in January-December 2022. Examination of the number of platelets in the counting chamber with 25 Medium Boxes of Erythrocytes and 1 Large Box of Leukocytes, from the 30 data obtained for each method after a different test using the Mann Whitney test obtained a significant p value of 0.234 > 0.05 so that it can be concluded that there is no difference in the number of platelets in 25 Medium Boxes of Erythrocytes and 1 Large Box of Leukocytes. This indicates that examination of the platelet count using 1 large box of leukocytes can be used as a manual examination method
Use Consumable Salt Concentration in Process Flotation Intestine Worm Eggs Rony Puasa; Febrianti Jakaria
Jurnal Media Analis Kesehatan Vol 14 No 2 (2023): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v14i2.270

Abstract

Salt is a chemical that is often used in families as a food flavoring or as a food preservative with the main substance being Sodium Chloride (NaCl). Re-fined salt as an ingredient for public consumption can be used in the laboratory as an alternative material to replace saturated NaCl (pa) for flotation of nematode worm eggs. The purpose of this study is to find out certain brands of consumable salt that can be used to float in-testinal nematode worm eggs. Method this research is a laboratory experiment where samples of consumed salt are treated and suspension is added and then the results of the treatment are observed microscopically. The results of this research is a good concentra-tion for the flotation process of 4 brands of consumable salt is 32% and the brand that can be used as a medium in the flotation process is the BTM salt brand. Conclusion this research is the BTM consumption salt brand provides good results in the flotation process by floating three species of intestinal nematose eggs, namely; round-worms, whipworms, hookworms (N. americanus and A. duodenale)
Potencial of Rice Bran Extract as a Basic Ingridient in Candida albicans Culture Media Rafika Rafika; Rahman Rahman; Ridho Pratama; Putri Sri Saqinah Sudirman; Mursalim Mursalim
Jurnal Media Analis Kesehatan Vol 14 No 2 (2023): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v14i2.275

Abstract

Rice bran is a natural material that contains nutrients that serves as a fungal growth factor, can be used as an alternative media base for fungal growth. The aim of this research is to determine the potential of rice bran as an alternative medium for the growth of Candida albicans. The research design employed an experiment that compared the growth of Candida albicans on rice bran extract media and Sabouraud Dextrose Agar (SDA) media. The sample is rice bran powder obtained from rice mills and rice bran extract was made using a multi-stage pretreatment method. The rice bran extract was used to create Rice bran Agar (RBA) and Rice bran Liquid (RBC) media, while SDA dan (SDB) media were also prepared. The media were inoculated with Candida albicans, incubated, and observed for 5 days. Data analysis included the measurement of fungal colony diameter using One Way Anova and Candida albicans biomass using an independent t-test. The research findings indicate that the average diameter of fungal colonies on RBA extract media was 10.4 mm, whereas it was 12.3 mm on SDA media. The Anova and Post Hoc Tukey HSD results demonstrated significant daily differences in colony diameter on RBA media, indicating notable Candida albicans growth. Conversely, there was no significant difference in colony diameter on SDA media. The biomass average of Candida albicans on RBC media was 0.82 g, compared to 0.45 g on SDB media. The Mann-Whitney test (p < 0.05) indicated a significant contrast in the average dry weight of Candida albicans biomass, with RBC media being heavier than SDB media. The conclusion is RBA and RBC media have the ability to grow Candida albicans which can be used as an alternative to SDA and SDB media.
Banana Tree Midrip Sap as an Antibacterial Staphylococcus aureus in Preventing Infection on External Wounds Intan Nur Aini; Dzafera Noor Muchlisha; I Wayan Ari Adinata; Nur Halimah; Rifa Nur Afifah Farhat; Yuliana Salman
Jurnal Media Analis Kesehatan Vol 14 No 2 (2023): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v14i2.281

Abstract

Wounds are damage to tissue continuity due to trauma from sharp or blunt objects, changes in temperature, chemistry, electricity, radiation, or animal bites. Wounds that are not handled properly can easily become infected, which can be caused by bacteria, one of which is Staphylococcus aureus. This study aims to determine the effectiveness of banana tree midrib sap as an antibacterial Staphylococcus aureus in preventing infection in external wounds. The research design used was experimental with a Completely Randomized Design (CRD) with a post test only control group design pattern. The research samples consisted of kepok banana tree midrib sap and Staphylococcus aureus bacterial culture using an accidental sampling technique. The antibacterial test method uses the well method by measuring the diameter of the clear zone on the media. The data collected was analyzed statistically using the Kruskal Wallis test and continued with the Post Hoc Kruskal Wallis test with a confidence level of 95%. The results showed that the sap of the banana tree midrib showed an influence on the Staphylococcus aureus bacteria with a value of sig. 0.01 (α<0.05). Banana tree sap (Musa Paradisiaca L) has an effect at concentrations of 15%, 30% and 60% on Staphylococcus aureus bacteria. These results are in accordance with the results of the qualitative phytochemical test of Kepok banana tree midrib sap which contains alkaloids, saponins, flavonoids and tannins which function as antibacterials. The conclusion of this research is that banana tree midrib sap has great potential as an antibacterial for Staphylocoocus aureus in preventing infections in external wounds
Sputum Sample Quality and Delay of TCM Examination and Microscopic Results from Tuberculosis Suspect Patient Yusianti Silviani; Ardy Prian Nirwana; Didik Wahyudi
Jurnal Media Analis Kesehatan Vol 14 No 2 (2023): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v14i2.319

Abstract

Tuberculosis (TB) is a disease with a mortality rate equivalent to 11 deaths/hour caused by Mycobacterium tuberculosis. Technological developments have been able to detect TB with the GeneXpert® MTB/RIF TCM (Rapid Molecular Test) examination. The aim of this study was to determine the effect of sputum sample quality and examination delays on TCM and microscopic results in patients suspected of having tuberculosis. The research design used in this study used experimental analytics with a cross-sectional approach. The sample in this study was 40 samples taken using quota sampling. Samples were examined using Microscopic and TCM methods. In the microscopic method, 3 treatment delays are carried out, namely immediately, 5 hours and 24 hours. The results showed that all samples experienced a decrease in the number of BTA + but a decrease in grade did not occur in all samples. The decrease in consistency began to appear at a delay of 5 hours. A decrease in BTA microscopic grading often occurs when the delay is from 5 hours to 24 hours. From the results of the Friedman test, a value of α= 0.000 (< 0.05) was obtained so that it can be concluded that there is no difference in the results of microscopic examination with TCM, but there is an influence of sputum quality on BTA results (+) and there is an influence of delay time on microscopic examination of BTA.
Formulation of 10% Glucose as a Mixture of Practicum Materials for Pregnancy Diabetes Mellitus Examination Skills in the Laboratory Andi Zulfaidawaty; Marsuki Marsuki; Wirawati Amin
Jurnal Media Analis Kesehatan Vol 14 No 2 (2023): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v14i2.362

Abstract

In laboratory-based learning, the use of formulated materials is crucial. A formulated glucose solution, with its predetermined composition, ready for use in observing Diabetes Mellitus (DM) scenarios in the laboratory, enables students to focus on direct experiences in simulating real-world situations. This enhances learning efficiency and provides greater opportunities for practical experiences. The objective of this research is to develop a formulation of a glucose 10% and aquadest mixture as synthetic urine to detect DM in laboratory skills activities. The experiment involved testing and observing a mixture of glucose 10% and aquadest. The resulting formula was tested using test strips and a heating procedure with Benedict's reagent. Five formulations, each prepared with a volume of 100 ml, yielded observations ranging from positive 1 to positive 4. Formula I, a mixture of 5 ml glucose 10% and 95 ml aquadest, resulted in a positive 4 observation. Formula II, with 4 ml glucose 10% and 96 ml aquadest, yielded a positive 3 observation. Formula III, with 3 ml glucose 10% and 97 ml aquadest, resulted in a positive 2 observation. Formula IV, with 2 ml glucose 10% and 98 ml aquadest, also produced a positive 2 observation. Formula V, a mixture of 1 ml glucose 10% and 99 ml aquadest, resulted in a positive 1 observation. It is recommended to prepare the mixture just before the activity, ensuring the cleanliness of containers to maintain the stability of the materials used. Further studies are needed to refine the formula with additional parameters
Analisis Hasil Pewarnaan Telur Cacing Menggunakan Pewarna Alternatif Filtrat Variasi Buah Nasir, Muhammad; Rafika, Rafika; Cleverine, Queen; Hasan, Zulfikar Ali; Nurdin, Nurdin; Askar, M.; Herman, Herman
Jurnal Media Analis Kesehatan Vol 15 No 1 (2024): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v15i1.372

Abstract

Infeksi parasit pada manusia sering kali disebabkan oleh cacing nematoda usus yang penyebarannya melalui tanah, dikenal sebagai Soil Transmitted Helminths (STH). Penyakit ini dapat didiagnosis dengan pemeriksaan mikroskopis menggunakan pewarna Eosin 2%. Beberapa bahan alami yang dapat digunakan sebagai pewarna alternatif untuk mewarnai telur cacing antara lain buah bit (Beta vulgaris L.), buah naga (Hylocereus polyrhizus), dan buah stroberi (Fragaria vesca) karena kandungan antosianinnya. Penelitian ini dilaksanakan pada tanggal 11-13 April 2023 di Laboratorium Parasitologi Poltekkes Kemenkes Makassar. Tujuannya adalah untuk mengetahui kemampuan buah bit, buah naga merah, dan buah stroberi dengan konsentrasi 1%, 2%, 3%, dan 100% sebagai pengganti Eosin 2% pada pemeriksaan telur cacing STH. Jenis penelitian ini adalah observasi laboratorik dengan teknik pengambilan sampel simple random sampling. Hasil penelitian menunjukkan bahwa pada konsentrasi 100%, buah bit (Beta vulgaris L.) memberikan lapangan pandang yang kontras, dengan telur cacing yang menyerap warna dan bagian telur yang terlihat jelas. Buah naga (Hylocereus polyrhizus) menghasilkan lapangan pandang yang kurang kontras, dengan telur cacing yang kurang menyerap warna dan bagian telur yang kurang terlihat jelas. Sementara itu, buah stroberi (Fragaria vesca) tidak cocok digunakan untuk pemeriksaan telur cacing. Penelitian ini menyimpulkan bahwa buah bit (Beta vulgaris L.) dan buah naga (Hylocereus polyrhizus) dapat digunakan sebagai pewarna alternatif dalam pemeriksaan telur cacing Soil Transmitted Helminths

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