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Ivan Permana Putra
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Jurnal Sumberdaya Hayati
Published by Institut Pertanian Bogor
ISSN : -     EISSN : 2477037X     DOI : DOI: 10.29244/jsdh.9.1.7-16
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
The "Jurnal Sumberdaya HAYATI" is a peer-reviewed and an open access journal published by Department of Biology, Bogor Agricultural University, Indonesia. The journal publishes significant and important research from all area of biodiversity, biosystematics, ecology, physiology, behavior, genetics and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 153 Documents
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Peran Ekstrak Kulit Batang Leea angulata Pada Tahap Proliferasi dalam Proses Penyembuhan Luka Kulit Mencit (Mus musculus): Role of Leea angulata Bark Extract on Proliferation Phase of Wound Healing Process in Mouse Skin (Mus musculus) Hidayah, Thauhidayatul; Hidayah, THAUHIDAYATUL HIDAYAH; Barlian, Anggraini
Jurnal Sumberdaya Hayati Vol. 7 No. 2 (2021)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.7.2.71-77

Abstract

Leea angulata is a commonly plant used by the Sasak people as a medicine to heal the wounds, but the effects of L. angulata on wound healing process has not been studied. The aim of this research are to determine the effects of L. angulata bark extracts on wound healing of mouse skin and its influence on the localization of FGF2. 1% of L. angulata bark extracts (P1%) on day 7 can accelerate wound closure amounted at 94.66%, Deep and Superficial Contraction Index of 0.96 and 0.1, epidermis Index and remodeling index of 1.55 and 0.81. L. angulata bark extracts stimulates the synthesis of FGF2 early (3rd day ) compared to the postive control group (5th day). FGF2 in P1% group wa found in epithelial cells in the hair follicles, fibroblasts cell in granulation tissue and epithelial cells in the epidermis. In the positive control group FGF2 was found in in epithelial cell of hair follicle, while in the negative control group, FGF2 undetectable. FGF2 in P1% group detected earlier than the positive control, showing the process of proliferation in P1% accelerated. The results confirm the L. angulata bark extracts accelerate the wound healing in proliferation phase and influence localization of FGF2 in the wound area. Keywords : Leea angulata, wound healing, FGF2, immunohistochemistry
Keragaman Permudaan Pohon di Area Sumber Air Blok Seda, Taman Nasional Gunung Ciremai: Diversity of tree seedlings in water springs area Seda Block, Mount Ciremai National Parks Slamet Arif Susanto; Dianti Mayadika Putri; Ilma Rahmawati; Made Ari Sanjaya; Sulistijorini
Jurnal Sumberdaya Hayati Vol. 7 No. 2 (2021)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.7.2.62-70

Abstract

Water availability in Mount Ciremai National Parks (MCNP) is influenced by sustainability of the vegetation. Therefore, tree regeneration around the water springs area needs to be investigated to maintain the sustainability of water springs ecosystem in the future. The purpose of this study was to analyzed and identify natural seedlings of tree regeneration around the water springs area Seda Block MCNP. The collections of vegetation and abiotic data was carried out on 9 sample plots measuring 3 m × 3 m which is purposively placed around water springs. Result show that vegetation in water springs dominate by seedlings Ficus sp., Ficus hemsleyana, and Trevesia sundaica. Shannon-Weiner diversity index in this location was moderate, but evenness and dominance index classified as high and low, respectively. Overall, seedling of trees dominated by orthodox seed-type and shade tolerant plant. The vegetation in this study dominated by plants categorized least concern (LC) based on IUCN red list. Microclimatic of study site affects 58.89% on abundance and distribution of tree seedlings. Therefore, the springs are in the Seda Block MCNP needs to be conserved and maintain its natural vegetation. Keywords: Mount Ciremai National Park, eco-hydrology, canonical correspondent analysis, conservation
Kekayaan Jenis dan Distribusi Anggrek Terestrial di Kawasan Hutan Dengan Tujuan Khusus (KHDTK) Sawala Mandapa, Majalengka: Species Richness and Distribution of Terrestrial Orchids in the Forest Area with Special Purpose (KHDTK) Sawala Mandapa, Majalengka Inama, Inama; Santoso, Nugroho Ponco; Kurniawan, Firman Heru; Raihandhany, Reza
Jurnal Sumberdaya Hayati Vol. 8 No. 1 (2022)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.8.1.1-7

Abstract

Anggrek merupakan salah satu jenis tumbuhan dengan tingkat keanekaragaman yang sangat tinggi, terdiri dari 28.000 jenis termasuk 763 marga. Sebagai salah satu negara megabiodiversitas, Indonesia memiliki setidaknya 5000 jenis Anggrek yang tersebar di berbagai tempat. Penelitian ini bertujuan untuk menginventarisasi jenis Anggrek Terestrial yang terdapat di KHDTK Sawala-Mandapa, Kabupaten Majalengka, Provinsi Jawa Barat. Penelitian Kualitatif ini menggunakan metode jelajah dengan ruang lingkup area Sawala-Mandapa dan dengan cara mencatat jenis dan titik koordinat tiap Anggrek Terestrial yang ditemui. Data primer yang diambil direkam menggunakan lembar catatan untuk mencatat jenis dan GPS untuk mencatat titik koordinat. Hasil yang diperoleh adalah terdapat setidaknya empat jenis Anggrek Terestrial yang ditemui antara lain; Geodorum densiflorum (Lam.) Schltr; Nervilia concolor (Blume) Schltr; Nervila campestris (J.J. SM) Schltr; dan Perystilus djampangensis J. J. Sm. Pada tingkat jenis, anggrek tanah di kedua area memiliki kecenderungan pola terkelompok dengan ukuran tiap kelompok pada tiap titiknya adalah 2-126 individu.
Diversitas Serangga yang Berasosiasi dengan Tanaman Jeruk Pamelo (Citrus maxima) : The Diversity of Insect Assemblages on Pummelo (Citrus maxima) Ria Anisa, Henta; Priawandiputra, Windra; Kahono, Sih; Atmowidi, Tri
Jurnal Sumberdaya Hayati Vol. 8 No. 1 (2022)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.8.1.8-13

Abstract

Jeruk pamelo (Citrus maxima) yang sering disebut jeruk bali merupakan spesies yang bernilai ekonomi tinggi. Di Indonesia, spesies ini memiliki beberapa kultivar. Tanaman ini berinterksi dengan banyak spesies serangga, baik sebagai predator, parasitoid, atau penyerbuk. Penelitian ini bertujuan mengukur keanekaragaman serangga yang berasosiasi dengan tanaman jeruk pamelo dan hubungannya dengan faktor lingkungan. Pengamatan keanekaragaman serangga menggunakan metode scan sampling pada 30 tanaman jeruk pamelo. Hasil penelitian didapatkan sebanyak 1.388 individu serangga yang termasuk dalam 17 spesies dalam 11 famili dan 5 ordo, yaitu Hemiptera, Orthoptera, Coleoptera, Hymenoptera, dan Lepidoptera. Spesies serangga yang dominan ditemukan adalah Camponathus sp. (Hymenoptera: Formicidae), Tetragonula laeviceps (Hymenoptera: Apidae), dan Chrysolina sp. (Coleoptera: Chrysomelidae). Jumlah individu serangga tinggi di pagi hari (pukul 07.00-08.00), sedangkan jumlah spesies tinggi pada pengamatan siang hari.
- Anatomi Daun Varietas Belimbing (Averrhoa carambola L.) Lokal di Taman Buah Mekarsari Bogor: Leaf Anatomy of Local Variety Starfruit (Averrhoa carambola L.) at Mekarsari Fruit Garden, Bogor Mardhatillah, Tila; Dorly, .; Ratna Djuita, Nina
Jurnal Sumberdaya Hayati Vol. 8 No. 1 (2022)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.8.1.27-33

Abstract

Sweet star fruit (Averrhoa carambola L., belimbing manis) is one of Indonesia’s most popular fruit plants. Anatomically, the characters of the local sweet star fruit of Mekar Sari Fruit Garden are not widely known, so this study aims to observe the anatomical structure of the leaves. The materials used were star fruit leaves of varieties Demak Jingga, Demak Kapur, Demak Kunir, Welahan, and Wulan. The leaves of star fruit varieties were made microscopic preparations in paradermal incisions using the whole mount method and transverse incisions using the paraffin method. The observation result of the paradermal incision of sweet star fruit leaves showed that the type of epidermal cell wall is straight and shallow grooved. The leaf stomata of five sweet star fruit varieties were paracytic. The calculation of the index value, size and density of stomata varied between varieties. The highest stomata density and index were found in the Welahan variety. The results of the transverse incision of sweet starfruit leaves showed that the five local sweet star fruit varieties have mesophyll tissue of the dorsiventral type. Wulan variety has leaves and mesophyll tissue which tends to be thicker than other varieties.
Regenerasi Tanaman Kitolod (Hippobroma longiflora (L.) G.Don) pada Kultur In Vitro: Plant Regeneration of Kitolod (Hippobroma longiflora (L.) G.Don) in In Vitro Culture ., Sumaryono; Nur`Aeni, Fitri; Ratnadewi, Diah
Jurnal Sumberdaya Hayati Vol. 8 No. 1 (2022)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.8.1.14-19

Abstract

Kitolod (Hippobroma longiflora (L.) G. Don) is a wild plant. Its flower is widely used as a traditional medicine.When this plant is utilized more intensively, there may be a shortage of the plant due to the lack of seed sources. This study aimed to obtain the best techniques and culture conditions for in vitro propagation of kitolod to provide a large number of planting materials. The experiments were arranged using a completely randomized design with two treatment factors and 10 replications for all experiments except in shoot rooting. Leaves and petioles were used as explant sources. Various combinations of benzilamino purine (BAP) and naphtalene acetic acid (NAA) were applied. Leaf explants in Murashige and Skoog (MS) medium enriched with 1 mg/L BAP and 0.1 mg/L NAA combination produced the highest number of adventitious shoots per explant, but 2 mg/L BAP + 0.1 mg/L NAA was more effective for shoot initiation and multiplication. The latter medium was also able to produce the tallest shoots, and presented 75% of successful rate over the acclimatization period. The best rooting was provided by MS medium added with 0.5-1.0 mg/L NAA.
Isolasi dan Karakterisasi Molekuler Gen 16S rRNA Bakteri Lipolitik Asal Limbah Kulit Biji Jambu Mete: Isolation and Molecular Characterization of Lipolytic Bacterial 16S rRNA Gene from Cashew Nutshell Waste Muhamad Azwar Syah
Jurnal Sumberdaya Hayati Vol. 8 No. 1 (2022)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.8.1.20-26

Abstract

Lipolytic bacteria attract great attention to various biotechnology industries because of their enzymatic potential. This study aims to isolate and identify lipolytic bacteria from cashew nutshell waste using the 16S rRNA gene as a molecular marker. Lipolytic bacteria were isolated using serial dilutions and inoculated on lipolytic media. A total of 3 isolates of lipolytic bacteria were obtained from cashew nutshell waste based on screening in LA Rhodamine B. The partial sequence of 16S rRNA gene from LB15 amplified using a pair of primers 63F and 1387R having a size of 1238 bp, while BL6 and BK6 were 1283 bp, respectively. Based on genetic distance analysis and phylogenetic reconstruction, we proposed that LB15 be identified as Burkholderia sp. with 99.92% similarity. In addition, because the 16S rRNA gene sequence similarity of BL6 was 99.87% with Paraburkholderia kururiensis strain 979, BL6 was classified as Paraburkholderia kururiensis. Then, isolate BK6 was identified as Ralstonia sp. with a similarity of 99.53%. The similarity value can be used as a reference in determining the identity of bacteria. A bacterium can be categorized as the same species if it has a similarity value of more than 99%.
Aktivitas Terbang Sebelum Migrasi dan Identifikasi Polen Pada Madu Apis dorsata di Kampar, Riau: Flight Activity Before Migration and Pollen Identification from Honey of Apis dorsata in Kampar, Riau Dyahastuti, Meis; Raffiudin, Rika; Widjaja, Mochammad Chandra; Afriani, Nita; Listyowati, Sri
Jurnal Sumberdaya Hayati Vol. 8 No. 2 (2022)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.8.2.34-41

Abstract

Ketersediaan sumber pakan yang rendah menjadi salah satu permasalahan yang dihadapi petani madu lebah A. dorsata di Kampar, Riau. Aktivitas lebah terdiri dari spatial learning atau pengenalan lingkungan, foraging atau mencari sumber pakan dan aktivitas migrasi. Penelitian bertujuan menganalisis aktivitas terbang lebah dan mengidentifikasi keanekaragaman polen yang ada di dalam madu. Pengamatan aktivitas terbang dilakukan dengan menghitung aktivitas keluar sarang, masuk sarang tanpa polen dan masuk sarang dengan polen selama 3 hari dengan 3 interval waktu. Polen didapatkan dari madu asal Kampar, Riau. Aktivitas lebah A. dorsata keluar sarang, masuk ke sarang tanpa polen dan masuk ke sarang dengan polen selama 3 hari paling tinggi berada pada pukul 11-12, yang merupakan bukan waktu terbang untuk mencari nektar dan polen pada umumnya. Aktivitas terbang meningkat seiring dengan meningkatnya suhu dan intensitas cahaya. Sebanyak 13 spesies tumbuhan yang menjadi sumber pakan A. dorsata terdiri atas habitus pohon, perdu, semak dan herba. Jumlah persentase polen paling banyak yang menjadi sumber pakan A. dorsata merupakan habitus pohon yaitu Pentace sp. (64%), Eucalyptus alba (22.9%) dan Elaeis guineensis (4%).
Aktivitas Antioksidan dan Antidiabetes In Vitro Ekstrak Air Kulit Batang Kayu Manis (Cinnamomum burmannii) Asal Kota Jambi: In Vitro Antioxidant and Antidiabetic Activities of Aqueous Extract of Cinnamon Bark (Cinnamomum burmannii) from Jambi Maulana, Farhan; Safira P, Ukhradiya Magharaniq; Safithri, Mega
Jurnal Sumberdaya Hayati Vol. 8 No. 2 (2022)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.8.2.42-48

Abstract

Cinnamon (Cinnamomum burmannii) is an herbal plant that has a function, one of which is lowering glucose in the blood and warding off free radicals that cause oxidative stress. This study aims to determine the inhibitory activity of the enzyme compound α-glucosidase and the suppression of DPPH free radicals using the first, second, and third replications of cinnamon bark water extract (C. burmannii) as a natural source of antidiabetic drug candidates. The extraction method uses the infundation technique and is carried out using a spectronometer. The highest yield was obtained in the extraction of the first repeat, which is with an average of 0.674%, the yield did not differ markedly (p>0.05) with the second and third repeats. The highest results of total phenolic testing were obtained in the second repeat extraction of 100.374 GAE g-1, the results differed markedly (p<0.05) with the first and third repeats. The highest results of testing antioxidant activity were obtained at the extraction of the first repeat which was 9.012 mg of AAE g-1, the results differed markedly (p<0.05) with the second and third repeats. The highest result of testing of enzyme inhibition activity α-glucosidase was found in the extraction of the first repeat which was 99.650%, the results did not differ markedly (p>0.05) with the second and third repeats.
The Effects of Root Endophytic and Arbuscular Mycorrhizal Fungi on Growth and Curcumin Content of Temulawak (Curcuma xanthorrhiza) : The Effects of Root Endophytic and Arbuscular Mycorrhizal Fungi on Growth and Curcumin Content of Temulawak (Curcuma xanthorrhiza) Putra, Sukma Triperdana; Iswantini, Dyah; Widyastuti, Utut; Fadillah, Wendi Nurul; Sukarno, Nampiah
Jurnal Sumberdaya Hayati Vol. 8 No. 2 (2022)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.8.2.49-56

Abstract

Temulawak (Curcuma xanthorrhiza) is known to be used as ingredient in Jamu and traditionally utilized to cure a range of illness. The global market demand for the rhizome of the temulawak affecting the urgency of sustainable rizhome production. The aim of this research was to analyze the effects of root endophytic and arbuscular mycorrhizal fungi on growth and rhizome curcumin content of the temulawak. There were five fungal inoculation treatments on C. xanthorrhiza seedling grown in sterilized and unsterilized growth media. They were root endophytic fungi A. niger (A), Glomus sp. (G), combination of A. niger and AMF Glomus sp. applied at the same time (GA) and at different time (G-A) and control. The experiment was conducted under greenhouse condition. The plant growth parameters, fungal colonization, and rhizomes curcumin content were measured. The result showed that there was a significant interaction between fungal inoculation and control treatments. In general, fungal inoculation on both sterilized and unsterilized growth media increased the plant growth and rhizomes curcumin content. On the sterilized growth media, C. xanthorrhiza inoculated by A. niger showed the best growth parameter. On the other hand, the best growth parameter on the unsterilized media was showed by C. xanthorrhiza inoculated by mixed fungal inoculums. The highest amount of curcumin content was showed by C. xanthorrhiza inoculated by Glomus sp. as a single inoculation.

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