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I G. Made Krisna Erawan
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krisnaerawan@unud.ac.id
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Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia
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Kota denpasar,
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INDONESIA
Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
Daun Binahong (Andredera cordifolia Steenis) Sebagai Alternatif Insektisida Terhadap Miasis yang Disebabkan Lalat Chrysomya bezziana (ANREDERA CORDIFOLIA STEENIS (BINAHONG) LEAF AS AN ALTERNATIVE INSECTICIDE AGAINST CHRYSOMYA BEZZIANA CAUSED MYIASIS) Ietje Wientarsih; Aulia Andi Mustika; April Hari Wardhana; Dodi Darmakusumah; Lina Noviyanti Sutardi
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.219 KB) | DOI: 10.19087/jveteriner.2017.18.1.121

Abstract

Larvae Chrysomya bezziana caused myiasis in most livestock in Indonesia. Drugs of choice for treating myasis are synthetic insecticides. Unfortunately the insecticides has negative effect on animal product, killed insect non target and insect resistenst. The research was conducted on the activity of ethanol extract of binahong leaves against various stages of C. bezziana larvae (L1, L2, and L3). Five treatments group tested were: group without any treatment (negative control), groups treated with ethanol extracts of binahong leaves 0.5%, 1%, and 2%, respectively, positive control were given coumaphos. The treatments were applied with five replications. Number of living larvae and pupae, pupae weight and number of imago were observed. The results demonstrated that 0.5% of the extract effective consentration which was able to kill the larvae (L1 and L2). Finally 2% of the extract was the most effective concentration which was able to kill larvae (L3) and decrease the pupae weight. Ethanol extract of binahong leaves was significantly able to reduce the growth of C. bezziana larvae due to contact and digestive effect of the active compounds contained in Anredera cordifolia leaf. ABSTRAK Miasis merupakan infestasi larva lalat yang terdapat pada jaringan hidup. Penyakit ini umum menyerang hewan maupun manusia. Obat yang digunakan sebagai pengendalian kasus miasis di lapangan sampai saat ini sangat terbatas dan menggunakan insektisida sintetik. Penelitian ini bertujuan melihat aktivitas ekstrak etanol daun binahong terhadap larva lalat Chrysomya bezziana pada stadium L1, L2, dan L3. Penelitian dibagi menjadi lima kelompok perlakuan dengan lima ulangan. Kelompok perlakuan terdiri dari kontrol negatif (tanpa pemberian obat), kelompok perlakuan yang diberikan ekstrak binahong dengan konsentrasi berturut-turut 0,5%, 1%, 2%, dan kelompok yang diberikan coumaphos (kontrol positif). Peubah yang diamati adalah jumlah larva yang hidup dan berkembang menjadi pupa, bobot pupa, dan daya tetas pupa menjadi imago. Hasil penelitian menunjukkan bahwa ekstrak etanol daun binahong pada konsentrasi 0,5% mempunyai efikasi sebagai larvasidal terhadap L1 dan L2 serta pada konsentrasi 2% merupakan konsentrasi terbaik sebagai larvasidal L3 larva C. bezziana. Ekstrak etanol daun binahong memiliki kemampuan menghambat larva lalat C. bezziana akibat dari efek cerna dan efek kontak bahan aktif yang terkandung di dalam daun binahong.
Penampilan Reproduksi dan Perkembangan Skeleton Fetus Mencit Setelah Pemberian Ekstrak Buah Nanas Muda Iriani Setyawati
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This experiment was performed to examine the effects of young pineapple (Ananas comosus) fruitextract on reproduction performance and foetus skeletal development given during organogenesis period.Twenty pregnant mice were randomly divided into four groups. The young pineapple fruit extract wastreated orally by gavage with doses 0% (control), 20%, 40%, and 80% since gestation day 6 to 15. Caesareansection were performed to pregnant mice on day 18 to remove foetuses. Observation covered : reproductionperformance (uterine weight before and after foetus removal), the foetus number (reabsorptions, dead/alive foetus), morphological of the foetus (weight and length of the litter, and malformations), and skeletaldevelopment (ossification number of metacarpus, metatarsus, vertebrae caudalis) and malformations ofcostae, sternebrae, and vertebrae. Statistical analysis was performed using Anova and Duncan’s MultipleRange Test.Teratogenic effects caused decreasing of earlier uterine weight and alive foetus, increasingdead foetus and hemorrhage, delayed skeletal ossification (decreasing ossification number of metacarpusand metatarsus) and caused costal malformation (intercostal fusion and convulated costal structure).Young pineapple fruit extract given to pregnant mice during organogenesis alter reproductive performancepregnant mice. The extract also caused morphological abnormalities (cretinism), hemorrhage, delayedossification on metacarpus and metatarsus, and costae malformation of the foetus.
Respon Antibodi Antikapsid pada Mencit yang Divaksin Vaksin Limpa dan Vaksin Kultur Virus Penyakit Jembrana Ni Luh Putu Manik Widiyanti; Ketut Suata; Nyoman Mantik Astawa; Hartaningsih -
Jurnal Veteriner Vol 10 No 2 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bali cattle are one of Indonesia national asset which need to be conserved as they have many advantages.They are however susceptible to many infections diseases such as jembrana disease. Currently, thedisease is prevented by vaccination using vaccine derived from jembrana disease virus (JDV)-infected balicattle. An alternative vaccine using JDV-infected lymphocyte culture is expected to increase the virusyield and is therefore likely to increase the antibody response in the vaccinated animals. A study thereforewas therefore conducted to compare the anti-capsid antibody response of Balb/c mice immunized withvaccine derived from the spleen of infected cattle (spleen vaccine) and those immunized with vaccinederived from infected lymphocyte culture (culture vaccine). As many as 16 female Balb/c mice weredivided into two groups, Each group was vaccinated 4 times weekly respectively with spleen and culturevaccines. The antibody response against the capsid protein of JDV was determined using enzyme-linkedimmunosorbent assay (ELISA) and the absorbance reading of mice sera from each group was compared. Tstudentand univariate analysis showed that the average absorbance reading sera of sera derived frommice vaccinated with spleen vaccine (0.15) was not significantly different from those vaccinated withculture vaccine (0.18). It appears that culture vaccine is able to induce anti-capsid antibody response ashigh as spleen vaccine.
Hambatan Ekspresi Vascular Endothelial Growth Factor oleh Ekstrak Daun Sambung Nyawa pada Endotel Membran Korioalantois (EFFECTIVITY OF SAMBUNG NYAWA LEAF EXTRACT TO INHIBIT VASCULAR ENDOTHELIAL GROWTH FACTOR EXPRESSION ON ENDOTHELIALS OF CHORIOALLANTOIC Iwan Sahrial Hamid; Dady Soegianto Nazar; Hermin Ratnani
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Angiogenesis is the new blood vessels formation normality and important on growth and developmentof individu. Angiogenesis also have contribution to carcinogenesis or uncontrolled and malignant cancercell development, become pathologic condition like inflammatory and infection. The purpose of this researchfor knew the effectivities of Gynura procumbens extract on various dose for inhibition Vascular EndothelialGrowth Factor (VEGF) expression. This research was done to effort cancer progress inhibition. However,angiogenesis is part of carcinogenesis causes. The Chorio Allantoic Membrane (CAM) methods was usedfor this aim. Eggs at the age of nine days were divided into 6 groups. Group I were negative control ofvehicle, group II were zero treatment: 60 ng bFGF which aplicated into paper dish. The next four groupswere extract of Gynura procumbens that divided in four dose: 60, 75, 90 and 110 µg + bFGF 60 ng whichapplicated into  paper dish.  At the twelve days old, VEGF expression analysis was done whichimunohystochemical method with anti VEGF’s antibody. The result of this research showed that therewas significant different (p<0.05) on give of Gynura procumbens extract to VEGF expression. The mostsignificant VEGF inhibition by Gynura procumbens extract with dose 110 µg. The conclusion on this studywas Gynura procumbens extract effective to inhibit the VEGF expression on CAM embrio chick.
Gambaran Fungsi Jantung Kelinci Domestik Secara Ekhokardiogram pada Anestesi Propofol dan Isoflurane Jangka Panjang (ECHOCARDIOGRAM PROFILE OF CARDIAC FUNCTION IN DOMESTIC RABBITS DURING LONG-TERM ANESTHESIA PROPOFOL AND ISOFLURANE) Sitaria Siallagan; Gunanti .; Deni Noviana
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Several major surgeries may require long time use of anesthetics and therefore increase the heartfunction suppression. The purpose of this study was to determine long term effects of anesthetic propofoland isoflurane on heart structure and function of domestic rabbits through observation of the blood flowvolume and contraction capabilities by M-mode echocardiography examination. This study used thecombination of propofol 12.5 mg/kg IV as an induction and isoflurane inhalation as the maintenanceanesthetic for a 12-hour observation. Parameters observed in this study were heart rate, wall thickness,internal chamber dimension, blood flow volume, fractional shortening, and ejection fraction. Results showedthat the left ventricle heart chamber dimensions during diastole (LVIDd) and systole (LVIDs) were between1.21±0.08 to 1.33±0.1 cm, and 0.87±0.08 to 0.98±0.08 cm respectively. Left ventricular wall thickness atdiastole (LVWd) and systole (LVWs) ranged from 0.24±0.04 to 0.28±0.04 cm, and 0.23±0.03 to 0.28±0.05cm respectively, while the thickness of the interventricular septa at diastole (IVSd) and systole (IVSs)ranged from 0.23±0.02 to 0.27±0.04 cm, and 0.28±0.06 to 0.30±0.05 cm. Heart rate ranged from 244.6±18.46to 266.4±24.55 beat/min. Heart flow volumes which included stroke volume (SV) and cardiac output (CO)ranged from 1.07±0.29 to 1.57±0.53 mL/beat and 0.27±0.07 to 0.39±0.14 L/min respectively. Fractionalshortening and ejection fraction ranged from 26±3 to 31±5% and 60±2 to 67±8% respectively. In conclusion,long term anesthesic of propofol and isoflurane combination induced minimum cardiac suppression indomestic rabbits.
Respons Antibodi terhadap Penyakit Tetelo pada Ayam yang Divaksin Tetelo dan Tetelo-Flu Burung (NEWCASTLE DISEASE/ND ANTIBODY RESPONSE OF CHICKENS VACCINATED WITH ND SINGLE AND COMBINED ND AND AVIAN INFLUENZA VACCINES) Gusti Ayu Yuniati Kencana; Nyoman Suartha; Mesakh Parlindungan Simbolon; Arini Nur Handayani; Steffi Ong; Syamsidar .; Aprillia Kusumastuti
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to investigate antibody response of specific pathogen-free (SPF) chickens vaccinatedwith single inactivated Newcastle disease (ND) vaccine and combined inactive ND and avian influenza(AI) vaccines and to known the efficacy of both vaccines. The vaccines used were killed ND vaccine andkilled ND-AI vaccine produced by PT. Sanbio Laboratories Bogor, West Java. SPF chickens were vaccinatedwith 3 different doses. Antibody titer of SPF chickens against ND virus were determined byhaemagglutination inhibition (HI) test. As many as 130 two week old SPF chickens were used and theywere divided into 2 groups (A and B) consisting of 60 chickens and 10 chickens were used as control withoutvaccine. Group A chickens were vaccinated with ND-K vaccine and group B were vaccinated with combinedkilled ND-AI vaccines. Each group was further divided into 3 subsgroups (1, 2 and 3) consisting 20 chickens.Subgroups 1, 2 and 3 were vaccinated intramuscularly respectively with intramuskular 1, 1/10 and 1/100doses of each vaccines. Antibody response of chickens against ND virus was examined before vaccinationand every three week after vaccination and was expresses as geometric mean titre (GMT) HI units. Theresult showed that the titre antibody against ND increased at the second week following the vaccination.The antibody titer against ND virus of chickens vaccinated single killed ND at the second week in eachdose were 6.05 GMT HI unit, 4.05 GMT HI unit, and 0.9 GMT HI unit. The antibody titre at the third week were 7.90 GMT HI unit ,5.40 GMT HI unit and 2.20 GMT HI unit. The antibody titre against ND virus ofchickens vaccinated with combined ND-AI vaccine at the second week were 6.30 GMT HI unit , 4.15 GMTHI unit , and 2.05 GMT HI unit. At the third week, the antibody titre against ND virus of chickensvaccinated with combined ND-AI vaccine in each subgroup were 7.45 GMT HI unit, 5.60 GMT HI unit , and2.40 GMT HI unit . It showed that the antibody titers at single doses of killed ND vaccine (7.90 GMT HIunit) and combined ND-AI vaccine (7.45 GMT HI unit) at the third week after vaccination were both stilleffective as both titres were above standard protective titre.
Cover, Redaksi, Daftar Isi JVet 17 (2) I Wayan Batan
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Cover, Redaksi, Daftar Isi JVet 17 (2)
Polimorfisme Protein Serum Darah Induk Sapi Beranak Kembar dan Tunggal pada Sapi Peranakan Ongole dan Keturunan Simental (BLOOD SERUM PROTEIN POLYMORPHISM OF THE COW DELIVERED TWIN OR SINGLE CALVES IN ONGOLE GRADE AND SIMENTAL CROSSBRED) Tri Yuwono; Irene Sumeidiana; Yon Soepri Ondho; Edy Kurnianto
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The twinning cow is presumable have difference in blood protein typology with the single cow. Thestudy investigated blood protein polymorphism in twinning and single of ongole grade and Simmentalcrossbred cows. The study used four twinning versus eight single ongole grade and seven twinning versussix single Simmental crossbred cows. Blood samples were analyzed by using marker ExactPro BroadRange (10-245kDa) and Sodium Dodecyl Sulphate-polyacrilamide Gel Electrophoresis method, to estimatethe loci of albumin (Alb), post-albumin (Pa), ceruloplasmin (Cp), transpherrin (Tf), and amylase-I (Amy-I).The genetic variation was analyzed based on the value of gene frequency, individual heterozigosity andaverage heterozigosity. In this study the twinning ongole grade cows had lower average heterozigosity(0.46) than the single ongole grade cows (0.49). The twinning Simmental crossbred cows had lower averageheterozigosity (0.35) than the single Simmental crossbred cows (0.40). The average heterozigosity ofongole grade cows had higher value than Simmental crossbred cows. The average heterozigosity rangedfrom 0.35-0.49, which was indicated high genetic variation in all cows. Higher gene frequency of allelealbumin A than albumin B influences the twinning trait in ongole grade and Simmental crossbred cows.
Pewarisan Karakter Fenotip Ayam Hasil Persilangan Ayam Pelung dengan Ayam Cemani Budi Setiadi Daryono; Iwan Roosdianto; Hendry Tri Sakti Saragih
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The germ plasm variability of Indonesian local chicken is well known and one species which known as“ayam Pelung” originated from West Java is famous for its meat. The present study aimed to observed thephenotype characters i.e. fur color and body weight in a crossbreed hybrids of Pelung and Cemani chickenas a potential meat type breed. Firstly, the breeding between Pelung (one male) and Cemani (4 Females)was performed in semi intensive cage. Secondly, experiment were undertaken using 4 groups of DOC (eachcontain 5 males DOC) : 1) DOC of broiler Cobb 500 vs broiler Cobb 500 ; 2) DOC of Pelung vs Pelung ; 3)DOC of Pelung vs Cemani ; and 4) DOC of Cemani vs Pelung. The animals were kept for 7 weeks (49 days)every week the animals body weight and fur color were recorded. The result showed that there weredifferences in the phenotype characters of between the different crossbreeds. The fur of 5 F1 (femaleCemani vs male Cemani) was black with average body weight 532 ± 39.294 g at week 7. Whilst the fur colorof majority (4/5) F1 (female Pelung vs male Cemani) was black with brown dots with average body weight570 ± 14.445 g at week 7. When Pelung was cross within the same species their F1 fur color was blendedbetween black, brown and whitish with average body weight 652 ± 33.846 g at week 7. It is concluded that,the parent stock in this case the Cemani play a major role in fur color character whereas there ware nodifferences in the body weight at the F1 between the two different parent stock (ie. Pelung, Cemani).
Gambaran Sitologi Cairan Peritoneal dan Sinovial Itik Bali (CYTOLOGIC FIGURES OF PERITONEAL AND SYNOVIAL FLUIDS IN BALI DUCKS) Iwan Harjono Utama; Sri Kayati Widyastuti; Anak Agung Ayu Mirah Adi; I Gusti Made Krisna Erawan; Ida Bagus Oka Winaya; Ida Bagus Komang Ardana; Achoiro Wati Rasid; Tyas Pandieka Yoga
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study aim was to observe cellular composition and morphology in peritoneal and synovial fluids ofbali ducks. This study used 50 ducks, which were composed of 21 males and 29 females aged 8 to 12 weeks.After being declared clinically healthy, the sample ducks were slaughtered and peritoneal fluid specimenfrom the abdominal cavity and the synovial fluid of joints tibiotarsal were collected. Then, in preparationfor the specimen, each of fluid was smeared and stained with Giemsa. Observations were made under amicroscope with a magnification of 1000 times and observations expressed in the average number of cellsper field of view observation. The results showed (duck population that most of the observed sample): inthe peritoneal fluid there was 36% eosinophils observed and 38% had heterophile cells with 0-3 cells perfield of magnification 1000x view. Similarly, 28% ducks had lymphocytes with 13-16 cells, and as much as28% of ducks had macrophage cells with 5- 8 cells in observation . In the synovial fluid, however, 64%sample ducks had monocytes, while 72% having lymphocytes with 1-18 cells each of view field withmagnification 1000x. In addition, in the synovial fluid, as much as 62% of ducks also observed had 1 to 3cells heterophiles and 60% ducks had eosinophils (polimorphonuclear cells) per view field observation.Macrophage cells that were found in the peritoneal fluid were mainly hyper granulated. It can be concludedthat the amount of mononuclear cells in both types of body fluids is likely more dominant compared to thenumber of polymorphonuclear cells.

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