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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
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Optimalisasi Performans Sapi Bali Melalui Pemberian Pakan Konsentrat dan Inseminasi Buatan di Kabupaten Buleleng, Bali Ni Luh Gede Budiari; I Putu Agus Kertawirawan; I Made Rai Yasa; I Nyoman Adijaya
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.201 KB) | DOI: 10.19087/jveteriner.2021.22.1.116

Abstract

Research related to the optimization of cow performans is done through concentrate feed and reproduction technology. This research was conducted in 2018 in Musi Village, Gerokgak Sub-district, Buleleng Regency, Bali. The study used a Completely Randomized Design (CRD) with 4 feed treatments and used 7 pregnant cows as replicates. The treatment is carried out when the cows enters 7 months pregnancy. Treatments tested: P0 cows given forage + rice bran 1 kg /cow/day + Bio Cas 5 ml/cow/day (mating with males), P1 = P0 cows mated with Artificial Imsemination (IB), P2 cows given forage + concentrates 1 kg/cow/day + bio cas 5 ml/cow/day (mating with a male), and P3 = P2 cattle mated with IB. Parameters observed that the parent body weight, weight gain calf, calf birth weight, weaning weight, calving interval, dry matter intake and feed rations convertions ratio. Data were analyzed by analysis of variance, if there are significant differences (P <0.05), followed by LSD 5%. The results showed that cows given feed concentrates and mated with IB (P3) result in weight gain of 0.52 kg/calf/ day was significantly higher (P <0.05) of P0 (0.40 kg/calf/day). Consumption of dry matter ration for P3 (2.59 kg) was significantly less (P <0.05) than P0 (2.87 kg) and resulted in the highest weight gain causing P3 FCR value of 4.98 was significantly lower than P0. Calf birth weight of all treatments showed no significant difference (P>0.05), but calf weight gain for P3 0.57 kg/calf/day was highest (P <0.05) compared to treatments P0 and P1 so that weaning weight calves P3 to 121.43 kg was significantly higher (P <0.05) than treatments P0 and P1. Estrus post partus for P3 is 2.87 months and calving interval is 12.36 months shorter than other treatments. This shows that the cows whose given concentrate feed mated through artificial insemination / males cattle produces the highest birth weight and calf weaning and short calving intervals.
SEKUENSING GEN MCR-1 DARI ESCHERICHIA COLI DAN SALMONELLA ENTERITIDIS RESISTAN KOLISTIN Maria Fatima Palupi; Ernes Andhesfa; Hera Maheshwari; Huda Salahuddin Darusman; Etih Sudarnika; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (229.83 KB)

Abstract

Gen mobilized colistin resistance (mcr) merupakan gen resistan kolistin sulfat yang bisa dipindahkan melalui materi genetik bergerak misalnya plasmid. Tujuan dari penelitian ini adalah mengetahui jenis plasmid gen mcr-1 yang berhasil dipindahkan dari satu isolat Escherichia coli resistan kolistin ke Salmonella Enteritidis ATCC 13076 (dua isolat), dan satu isolat E. coli O157:H7 resistan kolistin positif gen mcr-1. Sekuensing gen mcr-1 kemudian dianalisis di unit Biotek Balai Besar Pengujian Mutu dan Sertifikasi Obat Hewan dengan menggunakan basic local alignment search tools (BLAST) dan software MEGA7. Hasil sekuensing gen mcr-1 dengan panjang nukleotida 309 memiliki homologi tinggi dengan data referen dari Genbank pada gen mcr-1 yang terdapat dalam plasmid. Hasil sekuensing DNA gen mcr-1 sampel lebih cenderung termasuk pada tipe plasmid IncI2 atau IncHI2. Hal ini menunjukkan bahwa resistansi kolistin merupakan ancaman yang nyata karena gen resistan mcr-1 yang ada di Indonesia benar berada di plasmid sehingga mudah disebarkan ke bakteri lain. Kata kunci: mcr-1, plasmid, resistansi ABSTRACT The mobilized colistin resistance (mcr) gene is a colistin sulfate resistance gene that can be transferred through mobile genetic material such as plasmids. The purpose of this study was to determine the type of plasmid mcr-1 gene that was successfully transferred from an isolate of colistin-resistant Escherichia coli to Salmonella Enteritidis ATCC 13076 (two isolates), and one colistin-resistant E. coli O157: H7 that positive mcr-1 gene. The sequencing of the mcr-1 gene was then analyzed in the Biotek unit at National Veterinary Drug Assay Laboratory using basic local alignment search tools (BLAST) and MEGA7 software. The sequencing of the mcr-1 gene with nucleotide length 309 has a high homology with the reference data from Genbank on the mcr-1 gene contained in the plasmid. DNA sequencing of mcr-1 gene samples is more likely to belong to the IncI2 or IncHI2 plasmid types. This shows that colistine resistance is a real threat because the mcr-1 resistance gene in Indonesia is actually in the plasmid so it is easily spread to other bacteria. Keywords: mcr-1, plasmid, resistance
Gambaran Radiografi Penyakit Periodontal pada Kucing Jantan Lokal Berambut Pendek di Kampus Jatinangor Universitas Padjadjaran Ajeng Puspitasari; Amaliya Amaliya; Dwi Utari Rahmiati
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.783 KB) | DOI: 10.19087/jveteriner.2021.22.1.33

Abstract

Periodontal disease is the most common disease affecting pet animals including cats and dogs. This disease can cause pain and discomfort affecting the animal’s life quality. The purpose of this study is to evaluate periodontal disease from dental radiographs taken from male domestic short hair cats. Eight male domestic short hair cats from Universitas Padjadjaran Jatinangor campus were recruited for the study. This study observed several changes within the periodontium from dental radiography with simple quantification and analyzed using descriptive analysis. Dental radiographs were taken on the second right mandibula premolar (408). Several changes found in the dental radiography including widening of the periodontal ligament, bone loss and furcation involvement. This study shows varied changes in the periodontium due to the cat’s immune responses. The examined cats were experiencing widening of the periodontal ligament, bone loss and furcation involvement (37.5%, 25% and 87.5%) respectively. All cats examined were experiencing periodontal disease.
Perubahan Patologi Sistem Hepatobiliari Sapi Bali Terinfeksi Fasciola gigantica Ida Bagus Oka Winaya; Anak Agung Ayu Mirah Adi; I Ketut Berata; I Made Kardena; Ida Bagus Windia Adnyana; Ida Bagus Kade Suardana
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (160.416 KB) | DOI: 10.19087/jveteriner.2020.21.4.654

Abstract

Liver fluke infections is an important parasitic disease that common attack cattle, buffalo and others ruminant. The disease in the tropics is caused by Fasciola gigantica trematodes. Fasciolosis found in Bali cattle besides causing high economic loss also has the opportunity infect humans. This study aims to determine the pathology changes of Bali cattle hepatobiliary system infected by F. gigantica. In January2019 were examined a hundred of liver tissue Bali cattle. Based on the sex as many as 75 bali cattle are cows and 25 are bulls. On macroscopic examination was found fifteen bali cattles in gallbladder containing of F. gigantica. In positive Fasciolosis the surface of liver look uneven with enlargement of lymph nodes. The tissue of Bali cattle liver infected with F. gigantica is cut into 1x1x1 cm and put in a pot that has been filled with 10% neutral buffered formaline. The fixed tissue then processed in a tissue processor and stained with hematoxylin-eosin (HE). Microscopic examination was found bile ducts proliferation and blocked of the lumen accompanied by cholangio cirrhosis. Found longitudinal pieces of Fasciola giganticaliver fluke surrounded by fibrous connective tissue and multifocal coagulative necrosis with fibroblast proliferation. In some location the infiltration of neutrophil cells around the bile ducts can also be seen. Congestion, bleeding, and neutrophil infiltration are also seen in areas of necrosis. In the bile duct wall bleeding, adenomatus hyperplasia, infiltration of mononuclear cells with mild intensity and foci ofcalcification are found. It can be concluded that there was a change with severe intensity accompanied by chronic inflammation in the hepatobiliary system of Bali cattle with Fasciolosis.
Profil Leukosit Serta Imbangan Neutrofil dan Limfosit pada Kambing Peranakan Etawah yang Sedang Bunting Sus Derthi Widhyari; Setyo Widodo; I Wayan Teguh Wibawan; Anita Esfandiari; Chusnul Choliq
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136 KB) | DOI: 10.19087/jveteriner.2020.21.4.581

Abstract

This research aimed to determine the profiles of total leukocytes and neutrophil -lymphocyte ratio (N/L) in pregnant etawah crossbreed goats. Six etawah crossbreed goats around 3-6 years old and body weight around 30-50 kg were used in this study. Blood samples were taken from jugulars vein in non pregnant (K), and at gestational age 12, 14, 16 and 18 weeks. Blood is inserted into a tube that containsEDTA (Ethylene Diamine Tetraacetic Acid) anticoagulant to obtain whole blood. Whole blood were analyzed to total leukocyte cells and leukocyte types. The results showed that total leukocytes profiles tend to increase during pregnancy. Lymphocytes stable until 16 weeks of pregnancy and tends to decrease at the end of pregnancy. The N/L ratio increase at the end of pregnancy, this condition indicates an increase in the number of neutrophils accompanied by decreased lymphocyte cells and can be found in etawah crossbreedat 18 weeks of gestation.
POLA GERAKAN SPERMA SAPI SETELAH DIINKUBASI SECARA IN VITRO DALAM MEDIA FERTILISASI DENGAN IMBUHAN HEPARIN DAN/ATAU KAFEIN Achmad Setiyono; Ni Wayan Kurniani Karja; Mohammad Agus Setiadi; Ekayanti Mulyawati Kaiin
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Penelitian ini bertujuan untuk mengevaluasi penambahan heparin dan kafein secara tunggal maupun kombinasi terhadap status pola gerakan sperma selama proses kapasitasi in vitro. Semen beku yang sudah di thawing diinkubasi di dalam media fertilisasi saja atau ditambahkan dengan kafein 2 mM, heparin 10 µg/mL, dan kombinasi kafein 2 mM dan heparin 10 µg/mL selama 60 menit. Total motilitas, motilitas progresif dan pola gerakan sperma (VCL; LIN dan ALH) dievaluasi menggunakan CASA. Evaluasi dilakukan sebelum inkubasi atau 0, 15, 30 dan 60 menit setelah inkubasi di suhu 38.5 oC dan 5% CO2. Hasil penelitian menunjukkan VCL sperma tidak berbeda pada setiap kelompok dengan periode waktu yang berbeda (P>0.05), kecuali pada MF-Caf-2. persentase LIN pada MF-Caf-5 ditemukan lebih rendah dibandingkan kelompok lainnya sampai pada menit ke-30 (P<0.05), kemudian pada menit ke-30 nilainya sama dengan MF-Caf-2 (P>0.05). Segera sebelum inkubasi, ALH sperma pada MF-Caf-5 lebih tinggi daripada MF (P<0.05), tetapi tidak berbeda dengan MF-Caf-2 dan MF-Hep-10 (P>0.05). Sperma mengalami hiperaktif motilitas terjadi selama periode inkubasi dengan menambahkan kafein. Total motilitas sperma mulai menurun pada menit ke-30 pada MF dan dan MF-Hep-10 dan pada menit ke-60 pada MF-Caf-2 (P<0.05). Total motilitas sperma antar kelompok perlakuan pada periode waktu yang sama juga ditemukan tidak berbeda (P>0.05), tetapi motilitas progresif pada menit ke-60 lebih tinggi pada MF-Caf-5-Hep-10 jika dibandingkan dengan MF-Caf-2-Hep-10 (P<0.05). Dapat disimpulkan bahwa penambahan kafein secara tunggal atau dikombinasikan dengan heparin dapat menginduksi terjadinya hiperaktivasi dengan tidak terjadinya penurunan motilitas secara signifikan selama 60 menit selama periode inkubasi.
Melacak Kekerabatan Brucella Sp. Menggunakan Teknik Reaksi Rantai Polimerase dengan Primer Pendek Difa Widyasari; Eko Sugeng Pribadi; Fachriyan Hasmi Pasribu; Widya Septiningtyas; Jati Adiputra
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.704 KB) | DOI: 10.19087/jveteriner.2020.21.4.493

Abstract

Brucellosis is a zoonotic disease that has spread throughout the world and has an important impact on both human and animal health. The four species of Brucella that cause disease in humans are Brucella abortus, B. suis, B. melitensis and B. canis, and B. melitensis as the most pathogenic species. This Research used 46 samples were collected from Government Small Ruminants Abattoir in Bogor Regency. Thirty two spleen samples were examined by previous research and showed a positive result when were tested with CFT and PCR techniques, but sequencing has not yet been done. Fourteen serum and spleen samples were examined by the similar techniques. The Research aimed to determined the genetic relationship of Brucella sp. using a PCR technique with a specific short primer to B. mellitensis. Cloning technique was applied previously to five PCR positive samples before sequencing. Cloning and sequencing result of the Sample 91 showed higher homology to B. melitensis and B. abortus for 127 nucleotide lengths, 97.6% -100% and 99.2% -100% respectively. In the phylogenic tree, the Sample 91 was part of B melitensis sequences 1, 2,and 3 with accession numbers LT962930.1 and LT962936.1, B abortus sequences 1 and 2 with accession numbers CP033079.1 and B. abortus sequence 1 with accession number CP034695.1. Sample of 95, 97, 7, and 13 have lower homologies than Sample 91.
Identifikasi Bakteri pada Ikan Pindang Tongkol (Euthynnus affinis) di Pasar Tradisional Semarapura, Klungkung, Bali Purwaningtyas Kusumaningsih; Ni Made Diaris
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.669 KB) | DOI: 10.19087/jveteriner.2021.22.1.68

Abstract

Mackerel tuna (Euthynnus affinis) brine salting is a famous seafood product in Indonesia due to its good nutrition content. Bacterial contamination on this product may increase risk of foodborne diseases to happen, in addition to a decrease in quality and nutritionous value of the product. The main objective of this research was to identify bacteria contaminating the brine salting Mackerel tuna (Euthynnus affinis) sold in Tradisional Markets in Klungkung, Bali, Indonesia. Polymerase Chain Reaction (PCR) was apllied in the process of identification of bacterial contaminants. Samples were collected from 5 fish kiosks at the Tradisional market in Klungkung, Bali Indonesia. Each sample was cultured in Blood Agar or Nutrient Agar. Colonies with different morfology were selected, enriched in Tryptic Soy Broth (TSB), and their DNA was extracted before being amplified with 16S rRNA primers. This PCR products were then sequenced, and the results were compared with those available in the geneBank. Basic Local Alignment Search Tool was conducted with help of MEGA software version 6. Seven bacterial species were identified, and these included Serratia nematodiphila, Bacillus cereus, Shewanella seohaensis, Vibrio alginolyticus, Kurthia gibsonii, Enterobacter cloacae, and Staphylococcus sciuri. All but one Kurthia gibsonii are potensial pathogens in human. Staphylococcus sciuri is not commonly found in seafood. The present of such species in the mackerel tuna brine salting product was probably due to poor handling during the process and distribution.
Profil Hematologi dan Kadar Mineral Darah Tikus Putih pada Implantasi Bone Filler Produk Lokal Nadya Amanda Putri; Gunanti Gunanti; Dwi Gustiono; Agus Hadi Santosa Wargadipura; Deni Noviana
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.057 KB)

Abstract

Hidroksiapatit dan beta trikalsium fosfat merupakan biomaterial yang sering digunakan pada bidang dentistri dan ortopedi untuk teknik grafting karena bersifat biokompatibel dengan tubuh manusia. Permintaan akan bone graft yang meningkat membuat negeri kita mengembangkan produk lokal yang lebih murah, mudah didapat, dan biokompatibel. Tujuan dari penelitian ini adalah untuk mengidentifikasi hemokompatibilitas melalui evaluasi hematologi dan kadar mineral darah. Sejumlah 28 ekor tikus Sprague Dawley digunakan dalam eksperimen ini dan dibagi ke dalam empat grup. Grup I sebagai kontrol, grup II diimplantasi menggunakan bone graft komersil, grup III dan IV menggunakan bone filler berpori dan granul yang dikembangkan oleh Badan Pengkajian dan Penerapan Teknologi (BPPT). Tiap grup dibuat lubang defek sebesar 5 mm pada tulang kalvaria dan diambil darah pasca 30 hari implantasi. Parameter darah yang diambil yaitu sel darah merah, sel darah putih, kadar mineral kalsium dan fosfat. Hasil penelitian menunjukkan bahwa tidak ada perubahan yang signifikan pada pemeriksaan sel darah merah (P>0.05) dengan jumlah eritrosit berkisar antara 4.50-4.83x103?L walaupun terjadi kenaikan pada trombosit pada grup bone filler berpori dan granul pasca implantasi, dan pada sel darah putih (P>0.05) dengan leukosit berkisar antara 7.74-14x103?L. Pada pemeriksaan kadar kalsium dan fosfat juga tidak menunjukkan adanya perbedaan yang signifikan (P>0.05). Hal ini menunjukkan bahwa bone graft lokal tidak mengakibatkan peradangan kronik dan bersifat hemokompatibel.
Aktivitas Antiproliferasi Ekstrak Etanol Biji Kopi Hijau Robusta Lampung pada Sel Lestari Tumor Anjing Farra Sasmita; Ietje Wientarsih; Bayu Febram Prasetyo; Bambang Pontjo Priosoeryanto
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.353 KB) | DOI: 10.19087/jveteriner.2021.22.1.133

Abstract

This study aim at determining the activity of ethanol extract of Robusta green coffee beans as an antiproliferation agent in MCA-B1, MCM-B2, and Vero normal cell lines as a control. Ethanol extract of Robusta green coffee beans was obtained by maceration method, bioactive compounds in the extract were tested by phytochemical screening methods, testing levels of antioxidants were tested by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, cytotoxicity effects (LC50) were tested by the method Brine Shrimp Lethallity Test (BSLT) and antiproliferation activity were carried out by counting the number of cells after being tested with several extract concentrations namely 100, 250, 400, 550, 700 and 850 ppm and doxorubicin as a positive control with a concentration of 100 ppm. The results showed that the ethanol extract of Robusta green coffee beans contained flavonoids and tannins, had very strong antioxidant levels with IC50 values of 40.9923 ppm, cytotoxicity effects obtained LC50 values of 430.64 ppm, and the highest antiproliferation activity was achieved at a concentration of 850 ppm at 69.58 % in MCM-B2 tumor cells, 60.46% in MCA-B1 tumor cells, and 14.2% in Vero normal cells. Based on the results, the ethanol extract of Robusta Lampung green coffee beans has antiproliferation activity on the test tumor cells and is relatively non-toxic to normal cells.

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