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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
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Pemberian Ekstrak Oncom Hitam dan Merah Memperpanjang Siklus Estrus dan Mempertebal Endometrium Tikus Putih Hanifah Alshofa Nurul Aini; Desak Nyoman Dewi Indira Laksmi; Ni Luh Eka Setiasih; Steven Dwi Purbantoro
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.714 KB) | DOI: 10.19087/jveteriner.2020.21.4.565

Abstract

hytoestrogens are active compounds, derived from plants, which have a similar structure and function as estrogen. Phytoestrogens are commonly found in legumes. Oncom, which is assumed containing phytoestrogens, is one of the most famous legumes food from Indonesia and widely consumed daily in West Java. This study was aimed to determine the effect of oncom extract on estrous cycle, endometrium thickness, and the number of antral follicles in productive age rats (Rattus novergicus). This experimental study was using 21 three-to-four-month-old fertile female rats and divided into three groups. Group I (K) was considered as a control group without any treatment. Group II and III were treatment groups which were given black (H) and red (M) oncom extracts 0.005 g/g body weight, respectively, orally with a feeding tube for 14 days. The length of the estrous cycle was measured by performing vaginal swab with interval 12 hours after first treatment was given and during the treatment. Endometrium thickness and the number of antral follicleswere measured by collecting the organs uterus and ovary for histological purpose with paraffin method after rats were euthanized post-treatment oncom extracts for 14 days. Data were analyzed by ANOVA and continued with LSD test. The total length of estrous cycle of control group, black oncom extract group, andred oncom extract group was 107,43±3,16 hours, 141,43±15,36 hours, and 161,14±17,10 hours, respectively. The mean of endometrium thickness of control group, black oncom extract group, and red oncom extract group was 346,945±65,88 ?m, 485,740±86,69 ?m, and 533,904±78,93 ?m, respectively. The number ofantral follicles of control group, black oncom extract group, and red oncom extract group was 6,00±1,54, 8,43±2,99, and 9,14±2,72, respectively. Results showed that black and red oncom extracts had a significant effect on the length of estrous cycle and endometrium thickness in rats, yet there is no significant difference in the number of antral follicles. In summary, black and red oncom extracts had effects on the length of estrous cycle and endometrium thickness, yet there was no effect on the number of antral follicles.
Ethionamide Modulates of Thyroid Stimulating Hormone, Thyroxine, and Triiodothyronine Levels in White Ratstimulating Hormone (TSH), Thyroxine (T4), and Triiodothyronine (T3) Serum are potentially modulated Level by Ethionamide in Rats ronny lesmana; Fani Rahma Yenita; Prayudi Santoso; Hanna Goenawan; Arto Yuwono Soeroto; Yuni Susanti Pratiwi; Nova Sylviana; Iwan Setiawan; Vita Murniati Tarawan; Unang Supratman
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.585 KB)

Abstract

Multidrug-Resistant Tuberculosis (MDR-TB) infection is not solely caused serious impact for patient and cause long recovery process. Multidrug-Resistant Tuberculosis needs specific treatment approach using second-line TB drugs. Ethionamide as one of medication used in MDR-TB are known to cause greater side effects compared to the first-line drugs. One of side effect like hypothyroidism is remain unclear. There is limited study about role of ethionamide cause hypothyroidism. In this present study, 24 male, wistar rats were divided into three groups: ethionamide group; positive control positive (propylthiouracil) and negative control group (received distilled water only) and treated accordingly for 12 week. Under ketamine and xylazine anesthesia, blood sample were collected from retroorbital vein at 8 and 12 week, put in EDTA tube and stored -20Ë%C until use. After last sampling, rat were sacrificed using CO2 chamber. Our data showed that thyroid stimulating hormone (TSH) levels in ethionamide group is increased from 8 weeks to 12 weeks significantly, but there is no significant changes in control groups. Thyroxine (T4) levels is trend decreased from 8 weeks to 12 weeks compared to control groups. There is no change of Triiodothyronine (T3) levels. Effect of ethionamide on thyroid hormone (T3 and T4) levels was not prominent and there were no significant changes observed. Interestingly, we observed an increase of TSH level after ethionamide treatment. Taken together, 12 week treatment of ethionamide tablet might potentially modulate thyroid hormone (T3 and T4) levels that is reflected from an increase of TSH levels in the plasmas feed back negative mechanism.
Kelainan Jantung Kucing Secara Ultrasonografi Di Rumah Sakit Hewan Pendidikan Institut Pertanian Bogor Dan Klinik Hewan Di Jakarta Muflih Muhammad Rasyid; Mohamad Fakhrul Ulum; Siti Zaenab; Deni Noviana
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (648.438 KB)

Abstract

Heart diseases are the main cause of sudden deaths in cats. Those diseases can be diagnosed using ultrasonography. This study aims to investigate the cases of heart diseases of cats diagnosed by ultrasonography (echocardiography) in Veterinary Teaching Hospital, Faculty of Veterinary Medicine, IPB University and My Vets Animal Clinic, Kemang. Total thirty-one patients’ medical record files from veterinary teaching hospitals (eleven files) and clinics (twenty files) in 2013-2017 were indicated to have heart diseases. The datas acquired from patients’ medical records were signalement, B-mode, M-mode and color flow Doppler echocardiography results, and definitive diagnoses from medical records. Echocardiography examination was performed on conscious animals using a microconvex probe with the right parasternal view and left apical view. The heart diseases were divided into two categories, i.e. congenital and acquired, and each category describes age, sex, and race predispositions to heart diseases. In this study, congenital heart diseases found were aortic stenosis and ventricular septal defect. The acquired heart diseases found in this study were hypertrophic cardiomyopathy, endocardiosis, mitral regurgitation, tricuspid regurgitation, and pericarditis. The results of this study stated that acquired heart diseases had the bigger proportion in cats, compared to congenital diseases. The most prevalent heart disease was hypertrophic cardiomyopathy. As a conclusion, male, Persia and 7-12 years old cats are more susceptible to heart diseases.
Penggunaan Teknik Molekuler untuk Mengenali Dermatofita yang Diisolasi dari Hewan Kesayangan di Jakarta dan Bogor Dwi Endrawati; Eko Sugeng Pribadi; Agustin Indrawati; Eni Kusumaningtyas
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.365 KB) | DOI: 10.19087/jveteriner.2021.22.1.56

Abstract

Dermatophytosis is one of the superficial mycoses which causes skin health problems in pet animals. This study conducted molecular characterization using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) on specimens obtained from patients suspected of dermatophytosis in several clinics in DKI Jakarta Province and Bogor City. Fifty samples of skin scrapings from patients suspected of clinically dermatophytosis were collected and analyzed by conventional and molecular techniques. The Research aimed to identify dermatophyte that were isolated from pet animals using PCR-RFLP technique. The primers of ITS 1, ITS 4, Chytin Synthase, and cutting enzymes of Dde1 were used in this Research. Four specimens off 50 spesimens were tested positive using direct and culture PCR examination techniques. Based on the sequencing results, Microsporum canis was identified in four spesimens. Specimens that were positive for dermatophytes followed by RFLP using the Dde 1 enzyme. The results of the study showed that molecular techniques were a reliable way to determine the high-precision dermatophytes in diagnosing dermatophytosis. The Results also showed that molecular arrangement of B1 isolate was different from three other isolates.
Profil Leukosit Puyuh (Coturnix coturnix japonica) yang Mendapat Ekstrak Daun Singkong dalam Mengatasi Dampak Cekaman Panas Koekoeh Santoso; Anindita Sista Widyadhari; Okti Nadia Poetri; La Jumadin
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.141 KB)

Abstract

Penelitian ini bertujuan mengkaji potensi daun singkong (Manihot esculenta) dalam mengatasi cekaman panas pada puyuh terhadap parameter total leukosit, diferensiasi leukosit dan indeks stres. Penelitian ini dibagi menjadi empat kelompok. Kelompok kontrol serta Kelompok A, B, dan C masing-masing mendapat cekaman panas, kemudian diberi ekstrak klorofil daun singkong 5,292, 10,584, dan 21,168 mg/ 168 g bobot badan per oral selama 28 hari setelah diadaptasikan satu minggu. Parameter seperti total leukosit, diferensiasi leukosit dan indeks stres dilakukan setiap minggu. Hasil penelitian menunjukkan bahwa menunjukkan jumlah leukosit mengalami penurunan yang tidak berbeda nyata sejalan dengan peningkatan dosis ekstrak. Limfosit kelompok perlakuan ekstrak daun singkong (A, B, dan C) lebih tinggi (P < 0,05) dibandingkan kontrol, sedangkan heterofil kelompok perlakuan ekstrak daun singkong (A, B, dan C) lebih rendah (P < 0,05) dibandingkan kontrol. Monosit dan eosinofil kelompok perlakuan ekstrak daun singkong (A, B, dan C) cenderung lebih rendah dibandingkan kontrol. Pengamatan basofil menunjukkan hasil yang tidak berbeda nyata (P < 0,05) antara kontrol dengan kelompok perlakuan ekstrak daun singkong. Hasil penelitian lain menunjukkan bahwa semakin tinggi nilai H/L maka semakin tinggi tingkat stres hewan. Rasio H/L tertinggi terlihat pada kelompok kontrol, yang diikuti dengan kelompok perlakuan A, B, dan C. Penurunan tingkat stres puyuh teramati sejalan dengan peningkatan dosis ekstrak daun singkong yang diberikan. Simpulan pada penelitian ini adalah pemberian ekstrak daun singkong mampu menurunkan total leukosit serta rasio H/L. Selain itu, pemberian ekstrak daun singkong cenderung menurunkan monosit, heterofil, dan eosinofil serta meningkatkan limfosit.
Kualitas Antioksidan Senyawa Fitokimia dan Karakteristik Kimia Kulit Buah Matoa (Pometia pinnata) yang Dikeringkan Mira Andriani; Nahrowi Nahrowi; Anuraga Jayanegara; Rita Mutia; Theo Mahiseta Syahniar
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.226 KB) | DOI: 10.19087/jveteriner.2020.21.4.604

Abstract

This study was aimed to examine the influence of drying temperature on phytochemical compounds and chemical characteristic of matoa (Pometia pinnata) peels. This study used a completely randomized design with the drying temperature treatments which was divided into P0 = freeze drying (control), P1 =50Ë%C, P2 = 60Ë%C, and P3 = 70Ë%C, each treatment was dried for 48 hours. Variables observed were phytochemical compounds, water content, ashes content, crude protein, crude fat, crude fiber, IC50, malondialdehide/MDA and phenol. This study used analysis of variance followed by Duncan Multiple Range Test when the results showed significance dierence. It was calculated through Statistical Package for Social Science (SPSS) version 21.0. The results showed that the drying temperature influenced watercontent and IC 50. The phytochemical compounds of matoa peels were observed through qualitative screening included flavonoids, saponins, and tannins. The best results on the drying treatment of matoa peels was P1 (50Ë%C) which presented 9.47±0.14% water content, 3.74±0.07% ashes content, 4.89±0.03% crude protein, 0.46±0.04% crude fat, 34.42±2.16% crude fiber, 30.92±10.25 IC50, 12.85±1.49 mg/g MDA, and 0.85±0.23% phenol.
Kadar Estrogen Darah dan Tingkat Keasaman (pH) Mukus Serviks Sapi Aceh Memengaruhi Daya Penetrasi Spermatozoa Budianto Panjaitan; Ridho Pambudi; Romi Amansyah; Muslim Akmal; Tongku Nizwan Siregar
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (169.273 KB)

Abstract

This research has purposes to determine the relationship between estrogen levels and aceh cattle cervical mucus pH on spermatozoa penetration. This study used eight aceh cows aged 3-7 years old and had a body weight of 150-250 kg, had already been delivered, and has two regular cycles. All cows were given a synchronization treatment using prostaglandin F2á (PGF2á) at a dose of 25 mgs with single injection. A collection of cervical mucus was performed during the first cervical mucus discharge, which is about 8-16 hours after the initial onset of symptoms. Cervical mucus samples were collected by aspiration method. The pH of the cervical mucus was measured in the laboratory using a universal indicator pH (Nesco).The estrogen concentration was measured by enzyme linked immunosorbant assay (ELISA) method. Measurement of spermatozoa penetration power by calculating the distance of spermatozoa travel from the beginning where spermatozoa drops to reach the furthest distance. Spermatozoa penetration was observed with a microscope for 30 minutes. The data of the research were analyzed descriptively. The pH value of cervical mucus of aceh cattle was 6.0-9.0. The distribution data of pH cervical mucus of 6.0; 7.0; 8.0 and 9.0 were 1, 2, 4, and 1, average spermatozoa penetration at pH 6.0; 7.0; 8.0 and 9.0 is 10.0; 25.0±32.53; 10.5±15.70; and 12.0 mm. The estrogen concentration in viscosity of thick cervical mucus; medium; and dilute are 29.39±6.29; 23.24±4.62; and 30.93 pg/ml with a spermatozoa penetration strength rate of 14.8±19.11; 3.0±1.41; and 34.0 mm. The results of statistical analysis showed correlation between estrogen concentration (x) and spermatozoa penetration distance (y) in aceh cattle was 0.328 with regression equation y= 26.44 + 0.11x (P>0,05). In conclusion pH of aceh cattle cervical mucus affect the spermatozoa penetration. The best spermatozoa penetration is obtained at cervical mucus at pH 7. There is relationship enough between estrogen concentration and spermatozoa penetration in aceh cattle.
Karaterisasi Virus Avian Influenza Subtipe H5N1 Isolat Lapang Asal Bali Untuk Kandidat Vaksin Gusti Ayu Yuniati Kencana; I Nyoman Suartha; I Made Kardena; Arini Nurhandayani
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.103 KB) | DOI: 10.19087/jveteriner.2020.21.4.530

Abstract

A research on the isolation and characterization of the Avian Influenza H5N1 subtype field isolate has been carried out at the BSL-3 Laboratory of PT Sanbio Laboratories, Bogor. The aim of the study was to prepare a candidate for the H5N1 subtype Avian Influenza virus vaccine. Virus isolates were taken from field isolates from Bali. A total of seven field H5N1 AI subtypes from Bali were characterized in Bogor. The isolates were: isolate 3A, isolate 4A, isolate 9C, isolate 10 A, isolate 10 C, isolate P65, isolate P67. The passage of isolates was carried out on 9-day-old embryonic Specific Pathogenic (SPF) chicken eggs by injecting 0.1 mL of SPF isolates/eggs through the allantoic cavity. Each isolate was placed in five SPF eggs and then incubated in an incubator at 37 C and candled every day. Since day 2-4 post inoculation, embryo death has occurred. The eggs are harvested by their allantoic fluid and tested for haemagglutination test(HA/HI). The HI test results were confirmed by Reverse Transcriptase Polymerase Chain Reaction(RT-PCR) using the front primer FPHA232_13 (ATTGGTTAYCATGCAAAYAACTCG) and the back primer BPHA232_597 (GGAAAYATAGGTRGTTGGRTTYTGATAG) The results were five of the seven isolates were positive AI subtype B5 585 - 581 The five isolates of AI subtype H5N1 were subsequently sequenced, the results were all positive for AI virus subtype H5N1 clade 2.3.2. Each field isolate was given the name A / Chicken / Bali3A / GAY / 2019; A / Chicken / Bali9C / GAY / 2019; A / Chicken / BaliA4 / GAY / 2019; A / Chicken / Bali10A / GAY / 2016 and A / Chicken / Bali10C / GAY / 2019. One A / Chicken / Bali 9C / GAY / 2016 isolate was subsequently repeated 7 times until a stable H5N1 subtype AI virus titer was obtained. The results of matching with bioinformatics turned out that A / Chicken / Bali 9C / GAY / 2016 isolates had a kinship of 98.62% with AI subtype H5N1 Banyuwangi, amounting to 98.45% with AI subtype H5N1 Lamongan, amounting to 98.10% with AI-H5N1 Lumajang, 97.58% with AI-H5N1 Kediri, 97.07% with AIH5N1 Blitar, 96.72% with AI-H5N1 Denpasar, 96.72% with AI-H5N1 Buleleng and 96.72% with AI-H5N1Sukoharjo. The conclusion is one of isolate namely A / Chicken / Bali 9C / GAY / 2019 including AI subtype H5N1 clade 2.3.2, is’t stable at passage on SPF eggs, has a kinship of 96.72% with A / duck / Sukoharjo / BBVW-1428- 9/2012, the virus content is 106.9 ELD50 so it is potential for vaccine candidates.
Dukungan Terhadap Pengembangan Hijauan Indigofera di Kabupaten Manggarai Barat: Tinjauan Faktor-Faktor yang Memengaruhi Adopsi JOHANIS A JERMIAS; Cardial Leverson Leo Penu; Petrus Malo Bulu; Bernadete Koten; Melinda Moata; Mardianus Illi; Ewaldus Wera
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.475 KB) | DOI: 10.19087/jveteriner.2021.22.1.101

Abstract

Ruminant productivity is influenced by the quality and quantity of forage. Forage plant that is prospectively developed is Indigofera which has advantages such as high nutrient content, low crude fiber, being able to live in dry areas, and low anti-nutrient. However, as an innovation, adoption, cultivation, and utilization in a sustainable manner depend on several factors. This study was aimed to investigate the potential adoption of Indigofera forage in West Manggarai Regency with a focus on the factors that influence adoption. Data were collected through observations, individual interviews, Focus Group Discussions and study of documents. The number of respondent’s farmers was 59 from seven villages in five sub-districts. The results showed that in terms of the respondent’s characteristic factors, 96.61% were in the productive age, 94.7% had a formal education background, a sufficient number of a household member and a good perception of the Indigofera. From the socioeconomic condition factor, there are introductions of innovations through different patterns involving different actors with different results which can be used as an introduction model. From the characteristic factor, Indigofera forage has higher nutritional content compared to other popular forages, easy to live in the dry land, the process of breeding and cultivation is not difficult for the profession of farmers and has proven the results of development in other regions in Indonesia. Based on the facts and analysis, it was concluded that the Indigofera plant has the potential to be widely adopted and used by farmers in the West Manggarai Regency.
Kajian invitro campuran onggok dengan sari pepaya (Carica papaya L) dalam ransum Iman Hernaman
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Penelitian bertujuan mengetahui pengaruh campuran onggok dan sari pepaya (COSP) dalam ransum terhadap fermentabilitas dan kecernaan (in vitro) dan dilakukan secara experimental dengan menggunakan rancangan acak lengkap (RAL). Penelitian terdiri atas lima ulangan dengan empat perlakuan, yaitu : T0 (ransum kontrol), T1 (ransum dengan 10% COSP), T2 (ransum dengan 20% COSP) dan T3 (ransum dengan 30% COSP). Campuran tersebut dibuat dengan perbandingan 1:1 (Kg/L). Evaluasi ransum dilakukan secara in vitro. Data yang terkumpul, kemudian dilakukan analisis uji Duncan. Hasil penelitian menunjukkan bahwa penggunaan COSP sebanyak 30% dalam ransum dapat meningkatkan konsentrasi VFA dan kecernaan bahan kering (KcBK) dan kecernaan bahan organic (KcBO) (P<0,05), namun konsentrasi N-NH3 meningkat sampai perlakuan 20%. Penggunaan onggok yang diperkaya dengan sari pepaya dapat digunakan sebanyak

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