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Jurnal Veteriner

jvet
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Published by Universitas Udayana

ISSN : 14118327 EISSN : 24775665 DOI : https://doi.org/10.19087/jveteriner

Core Subject : Health,

Veterinary

Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki

Arjuna Subject : -

Articles 1,116 Documents

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Ekstrak Minyak Kelapa Daun Gamal (Gliricidia sepium) Sebagai Akarisida Botani untuk Skabies pada Kambing Dyah Haryuningtyas Sawitri; Yuningsih Yuningsih
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Scabies is a contagious parasitic skin disease caused by Sarcoptes scabiei. In Indonesia, this disease considerably remains a major problem in animal health, particularly in goats. The aim of this study is to investigate the effectivity of gamal leaves coconut oil extract (GLEC) for scabies treatment in goat. This research was divided into 2 parts: 1. Preparing Gamal Leaves Coconut Oil Extract (GLCE) which included4 stages of study: collection of gamal leaves; measurement coumarin level in gamal leaves; gamal leaves extraction with coconut oil; and extract stability test and 2. Application of GLCE in goats includes 3 stages of study : artificial S. scabiei infestations in goats; GLCE treatment in goats with 3 concentrations (12.5%, 25%, 50%); and drug safety testing. The results showed that the concentration of coumarin in the gamal leaves which was effectively for scabies drugs was > 1000 ppm. The 12.5%, 25%, 50% of GLCE concentrations are safe as botanical acariside and can reduce 100% of S.scabiei mite populations after 4x, 3x and 2x treatment at 1 week intervals, respectively. The 50% concentration of GLCE is safe, most effective, efficient and stable in storage for scabies drug.

Laporan Kasus: Fibrosarkoma Vagina Pada Anjing Golden Retriever Fauzi Ahmad; Albiruni Haryo; Fajar Shodiq Permata; Nurina Titisari
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

A Golden Retriever bitch, 10 years old, came to the Animal Hospital of Brawijaya University, Malang, Indonesia, her conditions was discharge from the vagina, stink smell, polyuria, polydipsia and swelling in the vaginal area that begin to appear one month ago. Physical examination results found her weight 30 kg, scored three for body condition score (scale 1-5), body temperature at 38ºC, pale mucous membrane, and Capillart Reill Time > 2 seconds. Hard mass was palpable in the vaginal area. Based on the anamnesis, physical examination and clinical symptoms, the bitch appeared have some different diagnosis such as pyometra, endometritis, vaginitis, and vaginal tumors. Hematology, radiograph, and histopathological laboratory tests were performed to confirm the diagnosis. Hematologic results showed the bitch suffered from leukocytosis of 41.7 x 103/?L, granulocytosis 36.5 x 103/?L, and hyperchromic microcytic anemia. The results of the radiograph examination showed a radiopaque solid mass in the vagina and uterus. The results of the histopathological examination found there were hyperchromasia nuclei and pleomorphism of fibroblast cell, and formation spindles cell of connective tissue. Based on the results of physical examination, hematological test, radiographic test, and histopathology, the dog was diagnosed with fibrosarcoma in the vagina. The treatment of this case was ovariohysterectomy and removal of the vaginal tumor. Early diagnosis and early treatment largely determine the patient’s prognosis, metastatic vaginal fibrosarcoma tumors showed poor prognosis.

Faktor-Faktor Risiko Kawin Berulang pada Sapi Potong di Kecamatan Licin, Kabupaten Banyuwangi, Jawa Timur Putri Nurmalia Damayanti; Trilas Sardjito; Ragil Angga Prastiya
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Nowadays, beef cattle farms in Licin Subdistrict, Banyuwangi Regency have problems in providing beef demand because the less of productivity. This problems occured due to the repeat breeding case and decrease the efficiency and productivity of livestock. It suspected because of the lack of hygine and sanitation from farmers and inseminator. This study aimed to correlate the factors and cases of repeat breeding on beef cattle in Licin Subdistrict, Banyuwangi Regency. It was a descriptive study which used 69 cow withsimple random sampling method. Primary data was obtained from direct observation of livestock, then continued with interviewing farmers using in-depth study system. While secondary data is obtained from the records of inseminator. It included the term of farming, farmer’s ability to detect estrus cycle, housingand the feeding of beef cattle. The collected data was analyzed by descriptive statistic, then processed by using SPSS 22.0. Chi Square Test (X2) was used to determine the relation between the causal factors and repeat breeding. The results of this study indicate the prevalence of repeat breeding in Licin Subdistrict, Banyuwangi Regency by 64%. All the factors such as the term of farming, farmer’s ability to detect estrus cycle, and feeding of beef cattle are correlated with the incidence of repeat breeding.

A MEGALOCYTIVIRUS INFECTION IN MARINECULTURE AND FRESHWATER FISH IN SEVERAL REGIONS IN INDONESIA Achmad Bahtiar Rifai
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.994 KB)

Megalocytivirus is a member of Iridoviridae family and causes very high economic losses in fish farming. Megalocytivirus has three species, Infectious Spleen and Kidney Necrosis Virus (ISKNV), Red Sea Bream Iridovirus (RSIV), and Turbot Reddish Body Iridovirus (TRBIV). This study aims to detect Megalocytivirus and identify the species (ISKNV, RSIV and TRBIV) in order to determine the latest status of Megalocytivirus infection in Indonesia. Samples were taken from marineculture include grouper, barramundi and silver pomfret, while in freshwater fishes were gourami, tilapia and freshwater ornamental fish from several regencies in Indonesia. Selected fishes were fishes with clinical signs such as the body looks dark, decreased appetite, mortality rate above 40%, less active in swimming (lethargy) and separating from other fish groups. The method for virus detection using coagglutination test and species identification using the Polymerase Chain Reaction (PCR) with the target gene Major Capsid Protein (MCP). All samples from North Sumatera Province showed negative results both serologically and molecular, while 11 other provinces gave varying results. All samples of freshwater fish were infected with ISKNV, fishes from marineculture were infected with ISKNV and RSIV. The TRBIV virus was not detected in all samples.

Siklus Reproduksi Lutung Jawa (Trachypithecus uratus) Betina Berdasarkan Kadar Hormon Estrogen dan Luteinizing Hormone Nurina Titisari; Aulia Firmawati; Ahmad Fauzi; Made Ayu; Ida Masnur; Iwan Kurniawan
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Currently, the information about Javan langurs female reproductive cycle in both cycle length and hormonal patterns is still widely unknown. Meanwhile, this information can be used as a guide in determining the fertile period of Javan langurs to conduct mating with higher pregnancy rate. The purpose of this study was to determine the reproductive cycle of adult female Javan langurs based on estrogen and luteinizing hormone (LH) levels. The experimental animals was originated from Coban Talun forest in Batu, which was undergoing a rehabilitation program. Feces samples of two female Javan langurs were taken for 40 days. Examination of estrogen and LH hormone levels in the feces was carried out by the enzyme-linked immunosorbent assay (ELISA) method and then analyzed descriptively. The results showed that fluctuations in the graphs of the hormones estrogen and LH appeared on days 8 to 16 and days 30 to 40 which showed the existence of two reproductive cycles. Whereas younger langur showed a pattern of hormonal fluctuations that are less regular, making identification of the reproductive cycle difficult. Based from the results, we concluded that the reproductive cycle of female Javan langur was estimated about 24 days with a hormonal pattern consisting of the follicular phase, the ovulation period and the luteal phase.

Gambaran Mikroskopis Berbagai Organ Merpati yang Menunjukkan Gejala Tortikolis dan Identifikasi Virus Penyakit Tetelo Berdasarkan Uji Agar Gel Presipitasi Risang Aji Dewandaru; Ferdinand Prayogo Cahyo Santoso; Sugiyono Sugiyono; Michael Haryadi Wibowo
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study aimed to determine the microscopic conditions of organs of pigeons that suffered from torticollis and identify the cause of disease in torticollis pigeons. Three pigeons showed torticollis symptoms were obtained as sample and marked as pigeon A, B and C, respectively. Isolation of pathogen with inoculation into embryonated pigeon eggs obtained from parents with no history of vaccination and not indicated ND. Identification of ND with agar gel precipitation (AGP) test. Observation of microscopical changes with histopathologic preparation using hematoxylin and eosin staining. Histopathological examination showed that pigeons was done severe neuritis vagus, trakheitis, pneumonia, air sacculitis, hepatitis, pankreatitis, nefritis, jejunoileitis, ileocolitis and orchitis. Perivascular cuffing found in brain. Degenerative changes found in the hepar and ren. Cardiac severe necrotic lesion, and depletion in white pulp area of spleen. Proventricular tissue showed flattening of mucosal epithelium, congestion lesions found in pulmonary tissue. The results of slow hemagglutination test of pigeon egg allantoic fluid, which tested for hemagglutination (HA) showed positive HA result with titers varying between 2, 32, and 64. Serological identification carried out with the AGP test on all culture samples against ND antiserum showing positive results of ND virus. Based on these dara, it can be concluded that the pigeons withsymptoms of torticollis is caused by ND virus.

Apoptosis Neuron Nukleus Merah pada Tikus Model Penyakit Parkinson Akibat Induksi Parakuat Diklorida Ayu Miftahul Khasanah; Niswah Nurul Fahma; Tri Wahyu Pangestiningsih
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Red nucleus plays a role in motoric control, so that disturbanced of neurons in this nucleus could be affected on motor impairment. Previous study reported that paraquat (PQ) could induced Parkinson disease, a neurodegeneratif disease that affected subtantia nigra pars compactca and clinically characterized by motoric disorders. Apoptosis is a programed cell death that physiologically normal for the body, but in large quantities will cause progresive cell death. This study aimed to observed apoptosis in rats RN neurons following by PQ injection. Ten male Wistar rats with the aged of 3 month, devided into two groups of five. Control group was given aquadest and treatment group was given PQ, injected intraperitoneally, dosage 7 mg/kg BW, twice a week for three weeks. At day 24th all rats were anesthetized using ketamin dosage 40 mg/kg BW and xylazine dosage 5 mg/kg BW, perfused intracardially using 4% paraformaldehyde and mesencephalons were collected. Mesencephalons were processed for histological preparations using the paraffin method and cut stereologically in coronal section, in 4 ?m thickness. Immunohistochemistry staining was done using caspase 3 antibody as a marker of apoptosis. Data were analyzed descriptively and quantitatively. The results in both groups showed RN neurons were large size and stelat or fusiform in shape. The percentage of apoptotic neurons in the RN of treatment group was significantly increased (80,4%±13,8%) compared to the control group (26,6%±18,32%) (P<0.01). In conclusion, following PQ exposurethere is a significant increased of apoptosis neurons number in the RN of rat model of Parkinson disease.

Antibiotic Resistant Pattern and Resistant Gene Identification of Staphylococcus aureus from Chicken Farm in Bogor Nabila Swarna Puspa Hermana; Usamah Afiff; Safika Safika; Agustin Indrawati; Fachriyan Hasmi Pasaribu
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Chicken is one of the important protein source in Indonesia. Moreover, the largest population of chicken layer and poultry in Indonesia is known situated at West Java province with Bogor manicipality as the main producer. The aims of this study were to determine the antibiotic resistance pattern of Staphylococcus aureus isolated from poultry and layer farm in Bogor. The study also identified gene encoded the resistance. Cloacal swab samples were collected from chicken broiler and layer farm in Bogor manicipality. The samples were then cultured in Mannitol Salt Agar (MSA) medium to obtain S. aureus. Suspected colony was then confirmed by biochemical test. Positive strains were tested against several antibiotics and the diameter of clear zone arround of colony was interpreted based on Clinical and Laboratory Standard Institute. Furthermore, the DNA from resistant strains were then extracted, followed by detection of the resistance gene by using polymerase chain reaction (PCR) method. A total of 14 isolates of S. aureus were positive from poultry farm, and 15 isolates from layer farm. Most of all were resistant to tetracycline, ampicillin, oxytetracycline, erythromycin and nalidixic acid. On the other hands, several strains were sensitive to gentamycin and chloramphenicol. The study showed 28 isolates out of them were multi-drug resistant. Resistant gene such as blaTEM, gyrA and tetA were also identified in some isolates except for ErmB gene which was found in isolates originated from poultry farm. In conclussion, S. aureus in both farm showed mostly multi-drug resistant to several antibiotics which were supported by identification of resistant gene among isolates.

The Difference of Results in Abdomen and Anus Area Measurement With Non-Contact Infrared Thermometer Anna Surgean Veterini; Nancy Margarita Rehatta; Hamzah Hamzah; Widijiati Widijiati; Sarmanu Sarmanu; Subijanto Marto Soedarmo; Widodo Jatim Pudjirahardjo; Annis Catur Adi; Heni Rachmawati; I Ketut Sudiana
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Study about sepsis is chalenging to get the right therapeutic method. One clinical signs of the sepsis mice model is changes in body temperature. An easy way to examine body temperature is using infrared non-contact thermometer. The aim of this study is to compare the body temperature obtained from infrared non-contact thermometer at the abdomen and anus area. We used male Mus musculus mice, body weight of 25–30 grams, were divided into 2 groups (control and treated groups). In the control group, mice were injected with NaCl 0.9% solution, with the amount of NaCl volume equal to LPS. In the treated group, mice were injected with 2.5 mg/kgBW of LPS intraperitoneally. Body temperature measurement was measured in abdomen (tabd) and anus (tan) area. Body temperature was measured at 8th and 24th hour after LPS or NaCl injection. Body temperature value result using tabd was higher than tan. LPS injection to mice produced an increase in body temperature but was not significant when compared to the control group (8th and 24th hour). The mean difference between tabd and tan in 8th hour control groups respectively were 2.12oC. The mean difference between tabd and tan in 24th hour control groups 4.6oC. The mean difference in treated groups (8th hour) was 4.66oC, while it was 4.77oC in the 24th hour treated groups. LPS could caused vasodilation of the vessels, the measurement area of non contact-infrared thermometer will be the most important factor to be considered; tan may closer to the physiology condition.

Deteksi Kontaminasi Bovine Viral Diarrhea Virus Pada Fetal Bovine Serum Yang Tersedia Secara Komersial Hastari Waryastuty; Sri Handayani Irianingsih; Raden Wasito
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Teknik diagnostik menggunakan kultur sel merupakan gold standard untuk menentukan penyebab utama suatu penyakit terutama yang disebabkan oleh virus seperti bovine viral diarrhea virus (BVDV). Prosedur kultur sel memerlukan suplementasi serum pada media pertumbuhan. Serum yang digunakan sebagai suplementasi umumnya berasal dari hewan spesies yang sama agar sel dapat tumbuh sesuai dengan habitatnya. Serum yang paling umum digunakan dalam teknik kultur sel adalah fetal bovine sera (FBS) karena kandungan faktor pertumbuhannya yang tinggi. Infeksi BVDV sering terjadi pada populasi sapi. Infeksi BVDV in utero menyebabkan materi (serum, sel dan jaringan) yang berasal dari fetus sapi terkontaminasi BVDV. Penelitian telah membuktikan bahwa dua dari sepuluh sampel FBS dengan merek dagang yang berbeda, menunjukkan hasil positif terhadap virus BDV dengan uji polymerase chain reaction. Isolasi terhadap BVDV dari FBS yang positif dilakukan pada kultur sel Madin-Darby bovine kidney (MDBK), dilanjutkan dengan pengecatan menggunakan uji immuno peroxidase monolayer assay, memberikan hasil negatif. Meskipun BVDV yang terkandung di dalam FBS sampel tidak bereplikasi, tetapi hasil ini tidak dapat diekstrapolasikan untuk setiap produk FBS yang tersedia secara komersial. Berdasarkan hasil tersebut disarankan adanya pengujian rutin terhadap kemungkinan terjadinya kontaminasi oleh BVDV yang dapat berpengaruh pada hasil diagnostik di suatu laboratorium.

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Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia

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Home Page

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Contact Name I G. Made Krisna Erawan

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Editorial Address Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia

Location Kota denpasar, Bali INDONESIA

Abstract

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Contact Name
I G. Made Krisna Erawan

Contact Email
krisnaerawan@unud.ac.id

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Journal Mail Official
-

Editorial Address
Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia

Location
Kota denpasar,

Bali

INDONESIA