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All Journal Bioma : Berkala Ilmiah Biologi Biotika: Jurnal Ilmiah Biologi El-Hayah : Jurnal Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Pengabdian Kepada Masyarakat Sakai Sambayan Jurnal Penelitian Sains Jurnal Biologi Tropis Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Biogenerasi Journal of Community Service and Empowerment Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun Jurnal Pengabdian Dharma Wacana BIOVALENTIA: Biological Research Journal Jurnal Mitrawarga JURNAL BIOLOGI PAPUA The International Journal of Tropical Veterinary and Biomedical Research BIO-SAINS | Jurnal Ilmiah Biologi

Priyambodo Priyambodo

Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Lampung

Author-ID : 1099743

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Populasi Telur Nyamuk Aedes sp. Pada Ovitrap yang Diberi Fermentasi Gula sebagai Atraktan Alami di Lingkungan Kampus FMIPA Universitas Lampung Sekar Pratiwi; Emantis Rosa; Priyambodo Priyambodo; Tugiyono Tugiyono
Jurnal Biologi Papua Vol 12, No 2 (2020)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Diseases transmitted by mosquito especially Aedes sp. there’s still happens in many areas of both city and village in Indonesia, until reducing Aedes sp. requires controlled effort. Attractant is a compound that can effect the behavior of mosquito even to decrease the population of mosquito directly. Sugar fermentation can be a natural attractant, this solution can produce carbon dioxide compound that can be a attract for mosquito, until mosquitoes are attracted to ovitrap. The purposes of this research was to determined the sugar fermentation to total of Aedes sp. eggs at ovitrap. The research design used is a Complete Random Design (CRD) with 5 repeated. Analysis of data using Analysis of Variance (ANOVA) and if there are the significant differences of treatment then test continued with BNT test signification levels α = 5%. The result is indicate that fermentation formula P1 (50 gr brown sugar+ 1 g yeast) found up to 15 mosquitos eggs at ovitrap with a percentage of 50% more than any other formula, ANOVA test indicate that the value of p<0,05 that’s mean there is none significant differences between the treatment. Key words: Aedes sp.; attractant; sugar fermentation

Pelatihan Penambahan Tanaman Sumber Nektar pada Masyarakat Pembudidaya Lebah Tanpa Sengat di Desa Harapan Jaya, Kecamatan Way Ratai, Kabupaten Pesawaran Nindy Permatasari; Priyambodo Priyambodo; Resti Puspa Kartika Sari; Lu'lu' Kholidah Fauziah
Jurnal Pengabdian Dharma Wacana Vol 3, No 4 (2023): Jurnal Pengabdian Dharma Wacana
Publisher : Yayasan Pendidikan dan Kebudayaan Dharma Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37295/jpdw.v3i4.379

Desa Harapan Jaya, Kecamatan Way Ratai, Kabupaten Pesawaran merupakan salah satu wilayah yang potensial untuk proses budidaya lebah tanpa sengat. Beberapa pembudidaya telah memulai proses budidaya lebah tanpa sengat. Namun, madu yang dihasilkan oleh lebah tanpa sengat yang dibudidayakan masih kurang optimal. Tujuan pelaksanaan pengabdian kepada masyarakat ini adalah untuk memberikan pemahaman dan pelatihan akan pentingnya tanaman sumber nektar dalam budidaya lebah tanpa sengat. Tanaman sumber nektar yang merupakan salah satu dari syarat vegetasi yang baik dalam pelaksanaan budidaya lebah tanpa sengat terdiri atas berbagai jenis dan varian. Kegiatan pengabdian kepada masyarakat dilaksanakan dengan metode ceramah, diskusi, penyerahan dan penanaman bibit tanaman sumber nektar. Kegiatan pengabdian masyarakat harus terus dimonitor agar memastikan upaya pelatihan yang dilaksanakan dapat berdampak positif terhadap produksi madu dari lebah yang dibudidayakan.

DNA Isolation on Captive Sumatran Elephant in Elephant Training Center, Way Kambas National Park: A First Step towards Its ID Card Elly L. Rustiati; Priyambodo Priyambodo; Siti Asiyah; Dedi Candra; Diah E. Anggraini; Elizabeth D. Krismuniarti; Eko Agus Srihanto; Liza Angeliya; Nuning Nurcahyani; Enny Saswiyanti
The International Journal of Tropical Veterinary and Biomedical Research Vol 3, No 1 (2018): Vol. 3 (1) May 2018
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Elephant Training Center (ETC) Way Kambas National Park (WKNP) was built to support human-elephantmitigation conflict. The small population of captive sumatran elephant in ETC WKNP need a comprehensivestrategy in order to maintain the genetic variation of each individual and avoid inbreeding drive. Currently, geneticstudies have opened new field studies in ecology, included conservation ecology. Patterns in variation of populationhas been investigated by molecular method supporting species conservation effort. The captive sumatran elephant’sID Card is a necessary in database building, which included morphology, health status, and genetic profile. Geneticprofile in each ID Card was filled by cytogenetic and molecular profile for RADP result, that initiated with DNAisolation. The DNA sources collected by blood sampling protocol described by Asiyah et al. (2016) from captivesumatran elephant in ETC, WKNP, and be carried to laboratory in cold condition. The DNA sources stored at 4oCand isolated following commercial protocol. The result of DNA isolation stored at -20oC until amplificationanalysis. DNA isolation was successfully done, for further individual genetic ID building.

Comparative Cytogenetic Study on Male and Female Captive Sumatran Elephant in Elephant Training Center, Way Kambas National Park Priyambodo Priyambodo; Elly L. Rustiati
The International Journal of Tropical Veterinary and Biomedical Research Vol 3, No 1 (2018): Vol. 3 (1) May 2018
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Sumatran elephant (Elephas maximus sumatranus) was one of five big mammals in Way Kambas National Park (WKNP). Cytogenetic study was a part of conservation effort for sumatran elephant, especially in conservation genetic field. Captive sumatran elephant’s blood in Elephant Training Center (ETC) WKNP was collected to karyotype analysis. Captive sumatran elephant’s chromosome was analyzed by squashing technique with pretreating cells in a hypotonic solution. The study of karyotypes were made by giemsa staining applied to captive elephant’s blood cell. The diploid number of captive sumatran elephant was 56 both in male and female. The karyotype analysis performed different fundamental number in male and female, both of total fundamental number (FN) and autosomal fundamental number (FNa). Chromosome of female sumatran elephant showed the FN = 8, and FNa = 6, but in male sumatran elephant FN = 7, and FNa = . The karyotypes of X chromosome was large submetacentric and Y chromosome was small acrocentric.. The karyotype presented here will compilled with DNA fingerprinting analysis of all captive sumatran elephant in ETC WKNP as references in future breeding policy.

Amplification of The GAPDH Gene from The Urine eDNA of Sumatran Rhino in Sumatran Rhino Sanctuary, Way Kambas National Park Priyambodo Priyambodo; Chicka Refina P. Putri; Elly L. Rustiati; Yeyen Kurniawati; Danisworo Zulkarnain; Dian Neli Pratiwi; Zulfi Arsan; Giyono Giyono; Ganis Mustikawati; Vindo R. Pertiwi; Sukatmoko Sukatmoko; Eko A. Srihanto; Enny Saswiyanti
Jurnal Biologi Tropis Vol. 23 No. 3 (2023): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i3.4989

Profiling the genetic character of each individual sumatran rhino is important in order to maintain individual viability and genetic variability of Sumatran rhinos. Non-invasive sampling is needed to minimize the disturbance of individual sumatran rhinos, this is due to the solitary character of sumatran rhinos and tend to be afraid to interact with humans. Until now, non-invasive sampling of sumatran rhinos in Way Kambas National Park (WKNP) has only been done through the source of the puddle. This study aims to amplify the GAPDH gene from sumatran rhino environmental DNA (eDNA) sourced from urine. Urine sampling was carried out on four of the eight sumatran rhino individuals in WKNP. DNA extraction of four sumatran rhino urine samples was carried out with reference to the DNeasy® Blood & tissue kit extraction protocol. Amplification of eDNA extraction results was carried out by polymerase chain reaction (PCR) using GAPDH primers. Visualization of extraction and amplification results from four individual sumatran rhinos at TNWK was tested qualitatively with 1% agarose gel electrophoresis. Electrophoresis results showed positive results after amplification of four individual sumatran rhino urine samples at sizes between 100-200bp. This study successfully amplified the GAPDH gene from four sumatran rhino individuals in WKNP based on qualitative tests. In further conservation efforts, it is necessary to explore eDNA extraction from other potential sources.

Optimizing honey production in stingless bee farming P. Priyambodo; Elly L. Rustiati; Nindy Permatasari; Mahfud Sidik; Indah A. Lestari; Ani A. Yani; Lousanja D. Sa'uddah
Journal of Community Service and Empowerment Vol. 4 No. 2 (2023): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jcse.v4i2.26431

Harapan Jaya Village, Way Ratai Subdistrict, Pesawaran Regency has very promising potential for cultivating trigona bees. Lecture and student from University of Lampung team conducted training on stingless bee cultivation in this village in 2022. At the end of 2022, several stingless bee colonies experienced deaths that were allegedly due to lack of nectar sources. This community service activity was carried out to provide understanding and training on vegetation management and adding stingless bee colonies. Activities are carried out with lectures, discussions, and hands-on practice. The results of the activities that have been carried out include the addition of seven types of nectar source plants and two stingless bee colonies. In addition, the community's understanding of the maintenance of stingless bee colonies and their supporting vegetation also increased. This is very supportive of long-term programmed in the achievement of SDGs, especially in the goals of life on land and no poverty.

KARAKTERISASI BEEBREAD DARI TUJUH SPESIES LEBAH TANPA SENGAT (STINGLESS BEE) BERDASARKAN KEANEKARAGAMAN POLLEN YANG DIKUMPULKANNYA Mahfud Sidik; Lousanja Dira Sa’uddah; Indah Ayu Lestari; Ani Andri Yani; Priyambodo Priyambodo
BIO-SAINS : Jurnal Ilmiah Biologi Vol 2 No 1 (2022): Bio-Sains Vol 2 No 1, Agustus 2022
Publisher : Fakultas Sains dan Teknologi, Universitas Islam As-Syafi'iyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6084/m9.figshare.23564679

Abstrak Lebah tanpa sengat atau stinglessbee akhir-akhir ini seringkali dipromosikan sebagai hewan budidaya yang sangat menjanjikan. Akan tetapi masih sangat sedikit literatur yang dapat digunakan dalam pembudidayaan lebah ini. Penelitian ini bertujuan untuk mengetahui karakteristik Beebread yang dikumpulkan oleh 7 spesies stinglessbee. penelitian ini dilaksanakan pada bulan Februari – Mei 2022, sampel beebread diambil langsung dari sarang lebah di PT Suhita Lebah Indonesia kota Bandarlampung dan dari pembudidaya lebah yang ada di desa Harapan Jaya kecamatan Wayratai kabupaten Pesawaran. Sampel dianalisis dengan metode asetolisis dan diamati menggunakan mikroskop serta dikelompokkan berdasarkan ketentuan Wingenroth (2001). Dari pengamatan yang dilakukan diketahui bahwa Spesies Tetrigona apikalis, Tetrigona vidua, dan Tetragonula reepeni memiliki karakteristik beebread monoflora dengan pollen didominasi oleh tumbuhan Cocos nucifera. beebread Tetragonula testaceitarsis dan Tetragonula levicep berkarakter biflora, serta beebread Heterotrigona itama dan Geniotrigona thoracica berkarakter multiflora. Dari ketujuh spesies lebah yang diamati mengumpulkan pollen dari tumbuhan C.nucifera dan memiliki persentase yang tinggi. Pollen C.nucifera dalam jumlah yang signifikan disebabkan oleh preferensi Stinglessbee untuk mencari makan pada bunga dengan konsentrasi gula tinggi yang tersedia sepanjang tahun. Kata Kunci: Beebread, Geniotrigona, Heterotrigona, Stinglessbee, Tetragonula, Tetrigona. Abstract The stingless bee or stinglessbee has recently been promoted as a very promising farm animal. However, there is still very little literature that can be used in the cultivation of these bees. . This study aims to determine the characteristics of beebread collected by 7 species of stinglessbee. This research was conducted in February – May 2022, the beebread samples were taken directly from bee hives at PT Suhita Lebah Indonesia, Bandarlampung city and from bee cultivators in Harapan Jaya village, Wayratai district, Pesawaran district. Samples were analyzed by acetolysis method and observed using a microscope and grouped according to the provisions of Wingenroth (2001). From the observations made, it is known that the species Tetrigona apikalis, Tetrigona vidua, and Tetragonula reepeni have the characteristics of beebread monoflora with pollen dominated by Cocos nucifera plants. Beebreads Tetragonula testaceitarsis and Tetragonula levicep have biflora characteristics, and Heterotrigona itama and Geniotrigona thoracica beebreads have multiflora characteristics. Of the seven species of bees observed, they collected pollen from the C. nucifera plant and had a high percentage. The significant amount of C. nucifera pollen was due to the Stinglessbee's preference for foraging on flowers with high sugar concentrations available throughout the year. Keywords : Beebread, Geniotrigona, Heterotrigona, Stinglessbee, Tetragonula, Tetrigona.

KAJIAN PERILAKU HARIAN BERUANG MADU (Helarctos malayanus) DI LEMBAGA KONSERVASI PT. LEMBAH HIJAU, LAMPUNG Rodliyah, Aini Robby; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Master, Jani; Ibransyah, Rasyid; Nasution, Irwan
BIOTIKA Jurnal Ilmiah Biologi Vol 21, No 2 (2023): BIOTIKA DESEMBER 2023
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v21i2.48771

Indonesia memiliki dua spesies beruang madu yaitu beruang madu sumatera (Helarctos malayanus malayanus) dan beruang madu kalimantan (Helarctos malayanus euryspilus). Lembaga Konservasi PT. Lembah Hijau, Lampung merupakan tempat berlangsungnya upaya perlindungan terhadap beruang madu. Penelitian dilakukan untuk mengetahui perilaku harian beruang madu di Lembaga Konservasi PT. Lembah Hijau, Lampung pada bulan Januari–Maret 2023. Metode penelitian yang digunakan adalah scan sampling, yang dilakukan dalam tiga tahapan meliputi survei pendahuluan, habituasi, dan observasi langsung. Pengamatan dimulai pada pukul 08.20–16.10 WIB pada hari kerja (Senin–Jumat), pukul 08.00–17.00 WIB hari libur (Sabtu–Minggu) dan pada hari libur nasional yang ditetapkan pemerintah di luar hari Sabtu–Minggu dengan interval waktu 10 menit dari area kunjungan pada kandang tampilan beruang madu. Perilaku tertinggi ke rendah secara berurutan meliputi perilaku sosial (15,22%), perilaku berjalan/jelajah (15,14%), perilaku tidur/istirahat (13,42%), perilaku bermain (8,3%), perilaku makan (6.61%), perilaku menelisik (4,45%), perilaku minum (1,49%), perilaku urinasi (0,92%), perilaku bersuara (0,77%), dan perilaku defekasi (0,35%). Beruang madu yang sedang dalam masa kawin menjadi faktor tingginya perilaku sosial dan berjalan. Defekasi dan urinasi dengan posisi yang berbeda pada masing-masing individu menjadi penanda masih adanya sifat soliter pada beruang madu.

Identification of Visual Character of Leaves with Sumatran Rhino's Urine in the Sumatran Rhino Sanctuary Way Kambas National Park as Potential Source of eDNA Priyambodo, Priyambodo; Putri, Chicka R.P.; Rustiati, Elly L.; Kurniawati, Yeyen; Zulkarnain, Danisworo; Pratiwi, Dian N.; Arsan, Zulfi; Giyono, Giyono; Mustikawati, Ganis; Pertiwi, Vindo R.; Sukatmoko, Sukatmoko; Srihanto, Eko A.; Saswiyanti, Enny
El-Hayah:Jurnal Biologi Vol 9, No 2 (2023): EL-HAYAH (VOL 9, NO 2 Maret 2023)
Publisher : Program Study of Biology, Science and Technology Faculty, UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v9i2.21179

The sumatran rhinoceros is one of the large endemic mammals to the island of Sumatra, with critically endangered status by the IUCN. Currently, the existence of the sumatran rhino is challenging to find in its natural habitat. Therefore, it is necessary to develop indirect monitoring to maintain and increase the stability of the population in nature. Monitoring through camera traps has been used in several locations. Another method is carried out by identifying traces and remains of the sumatran rhino in natural habitats, for example, through urine samples. The sumatran rhinoceros has the behavior of urinating, namely through urine spray. Urine sprayed by the sumatran rhino will hit plant parts in its habitat, including leaves. This study was aimed to identify the visual characteristics of leaves containing samples of sumatran rhino urination as a potential source of eDNA material. The results showed the leaves characteristics containing the sumatran rhino's urine on the 0, 1, and 2 days after the sumatran rhino urination. The difference in visual observations of the sumatran rhino's urine spray attached to the leaves can be seen through the change in the color of the urine fluid, which is getting more and more concentrated. Visualization of leaves containing sumatran rhino urine can be used to recommend visual leaf characteristics that can be taken as similar samples in natural habitats.

Plankton Community Structure as a Bioindicator of Water Quality in the Way Sekampung River- the Segment of Rulung Helok Tugiyono, Tugiyono; Ayuni, Ubaid Jan; Priyambodo, Priyambodo; Susanto, Gregorius Nugroho
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 2 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i2.293

Planktons are microorganisms that live in aquatic habitats. Plankton is used as a quality bioindicator of a water body. Way Sekampung flows to several areas in Lampung Province. The existence of activities around the watershed such as settlements, agriculture, and industrial waste disposal is thought to affect the water quality of the Way Sekampung River. The purpose of this study was to determine the water quality of Way Sekampung River based on plankton community structure consisting of abundance index, diversity index, uniformity index, and dominance index. Water sample data were analyzed using chemical parameters namely pH, BOD, DO, Phosphate, Nitrate, and physical parameters namely temperature and Total Suspended Solid (TSS) which are used to determine the status of water quality standards. This research was conducted at the beginning of the dry season (April) and the beginning of the rainy season (October). Sampling used a survey method based on the Way Sekampung River segment. Sampling points were carried out before and after the Argoguruh Dam. The results showed that the water quality of Way Sekampung River at the beginning of the dry season (April) and the beginning of the rainy season (October) were moderately polluted based on the analysis of plankton community structure such as abundance index, diversity, uniformity, and dominance.

Co-Authors Agustin, Meli Agustrina, Rochmah Alvin Wiwiet Susanto Alvin Wiwiet Susanto Amaliya Sabana Ani A. Yani Ani Andri Yani Ani Andri Yani Aprilia Eka Putri Arsan, Zulfi Ayuni, Ubaid Jan Bambang Irawan Bondan Pergola Chicka Refina P. Putri Danisworo Zulkarnain DEDI CANDRA Diah E. Anggraini Diah Esti Anggraini Dian Neli Pratiwi Dian Neli Pratiwi Dian Neli Pratiwi Eko A. Srihanto Eko Agus Srihanto Eko Agus Srihanto Eko Agus Srihanto, Eko Agus Elizabeth D. Krismuniarti Elly L. Rustiati Elly L. Rustiati Elly L. Rustiati Elly Lestari Rusitati Elly Lestari Rustiati Emantis Rosa Enny Saswiyanti Enny Saswiyanti Enny Saswiyanti Erdi Suroso Eti Ernawiati Eti Ernawiati Eva Yunita Favorisen R. Lumbanraja Firwantoni Firwantoni Ganis Mustikawati Gina Dania Pratami, Gina Dania Giyono Giyono Giyono Giyono I Gede Swibawa Ibransyah, Rasyid Indah A. Lestari Indah Ayu Lestari Indah Ayu Lestari Intan Diani Fardinatri Irwan Nasution Jani Master Jani Master, Jani Junaidi Junaidi Krismuniarti, Elisabeth Devi Kurniawati, Yeyen Lestari, Septi Wahyu Lili Chrisnawati Liza Angeliya Lousanja D. Sa'uddah Lousanja Dira Sa’uddah Lousanja Dira Sa’uddah Lu'lu' Kholidah Fauziah Mahfud Sidik Mahfud Sidik Mahfud Sidik Martha Lulus Lande Masniar Vina Kesti Melya Riniarti Mustikawati, Ganis Nindy Permatasari Novianasari, Tika Nugraha, M. Aqwam Nuning Nurcahyani Patrice Kevin Marcus Pertiwi, Vindo R. Pratiwi, Dian N. Pratiwi, Dian Neli Putri Ayu Febrina Putri, Chicka R.P. Resti Puspa Kartika Sari Rochmah Agustrina Rochmah Agustrina Rodliyah, Aini Robby Romaya Wulan Suciningtyas Rustiati, Elly L. Sandra, Shifa Saswiyanti, Enny Sekar Pratiwi Siti Asiyah Srihanto, Eko A. Sukatmoko Sukatmoko Sukatmoko, Sukatmoko Susanto, Alvin Wiwiet Susanto, Gregorius Nugroho Thesalonika, Natasya Tristiyanto Tristiyanto Tugiyono Tugiyono Tugiyono Tugiyono Vindo R. Pertiwi Virnarenata, Elsa Warsono Warsono Winarno Winarno Winda Rahmawati Yanti Yulianti Yeyen Kurniawati Yuni Tinasari Zulfi Arsan Zulkarnain, Danisworo

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