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Populasi Telur Nyamuk Aedes sp. Pada Ovitrap yang Diberi Fermentasi Gula sebagai Atraktan Alami di Lingkungan Kampus FMIPA Universitas Lampung Sekar Pratiwi; Emantis Rosa; Priyambodo Priyambodo; Tugiyono Tugiyono
Jurnal Biologi Papua Vol 12, No 2 (2020)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.059 KB) | DOI: 10.31957/jbp.1117

Abstract

Diseases transmitted by mosquito especially Aedes sp. there’s still happens in many areas of both city and village in Indonesia, until reducing Aedes sp. requires controlled effort. Attractant is a compound that can effect the behavior of mosquito even to decrease the population of mosquito directly.  Sugar fermentation can be a natural attractant, this solution can produce carbon dioxide compound that can be a attract for mosquito, until mosquitoes are attracted to ovitrap.  The purposes of this research was to determined the sugar fermentation to total of Aedes sp. eggs at ovitrap.  The research design used is a Complete Random Design (CRD) with 5 repeated. Analysis of data using Analysis of Variance (ANOVA) and if there are the significant differences of treatment then test continued with BNT test signification levels α = 5%.  The result is indicate that fermentation formula P1 (50 gr brown sugar+ 1 g yeast) found up to 15 mosquitos eggs at ovitrap with a percentage of 50% more than any other formula, ANOVA test indicate that the value of p<0,05 that’s mean there is none significant differences between the treatment.  Key words: Aedes sp.; attractant; sugar fermentation
Pelatihan Penambahan Tanaman Sumber Nektar pada Masyarakat Pembudidaya Lebah Tanpa Sengat di Desa Harapan Jaya, Kecamatan Way Ratai, Kabupaten Pesawaran Nindy Permatasari; Priyambodo Priyambodo; Resti Puspa Kartika Sari; Lu&#039;lu&#039; Kholidah Fauziah
Jurnal Pengabdian Dharma Wacana Vol 3, No 4 (2023): Jurnal Pengabdian Dharma Wacana
Publisher : Yayasan Pendidikan dan Kebudayaan Dharma Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37295/jpdw.v3i4.379

Abstract

Desa Harapan Jaya, Kecamatan Way Ratai, Kabupaten Pesawaran merupakan salah satu wilayah yang potensial untuk proses budidaya lebah tanpa sengat. Beberapa pembudidaya telah memulai proses budidaya lebah tanpa sengat. Namun, madu yang dihasilkan oleh lebah tanpa sengat yang dibudidayakan masih kurang optimal. Tujuan pelaksanaan pengabdian kepada masyarakat ini adalah untuk memberikan pemahaman dan pelatihan akan pentingnya tanaman sumber nektar dalam budidaya lebah tanpa sengat. Tanaman sumber nektar yang merupakan salah satu dari syarat vegetasi yang baik dalam pelaksanaan budidaya lebah tanpa sengat terdiri atas berbagai jenis dan varian. Kegiatan pengabdian kepada masyarakat dilaksanakan dengan metode ceramah, diskusi, penyerahan dan penanaman bibit tanaman sumber nektar. Kegiatan pengabdian masyarakat harus terus dimonitor agar memastikan upaya pelatihan yang dilaksanakan dapat berdampak positif terhadap produksi madu dari lebah yang dibudidayakan.
DNA Isolation on Captive Sumatran Elephant in Elephant Training Center, Way Kambas National Park: A First Step towards Its ID Card Elly L. Rustiati; Priyambodo Priyambodo; Siti Asiyah; Dedi Candra; Diah E. Anggraini; Elizabeth D. Krismuniarti; Eko Agus Srihanto; Liza Angeliya; Nuning Nurcahyani; Enny Saswiyanti
The International Journal of Tropical Veterinary and Biomedical Research Vol 3, No 1 (2018): Vol. 3 (1) May 2018
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (189.698 KB) | DOI: 10.21157/ijtvbr.v3i1.11368

Abstract

Elephant Training Center (ETC) Way Kambas National Park (WKNP) was built to support human-elephantmitigation conflict. The small population of captive sumatran elephant in ETC WKNP need a comprehensivestrategy in order to maintain the genetic variation of each individual and avoid inbreeding drive. Currently, geneticstudies have opened new field studies in ecology, included conservation ecology. Patterns in variation of populationhas been investigated by molecular method supporting species conservation effort. The captive sumatran elephant’sID Card is a necessary in database building, which included morphology, health status, and genetic profile. Geneticprofile in each ID Card was filled by cytogenetic and molecular profile for RADP result, that initiated with DNAisolation. The DNA sources collected by blood sampling protocol described by Asiyah et al. (2016) from captivesumatran elephant in ETC, WKNP, and be carried to laboratory in cold condition. The DNA sources stored at 4oCand isolated following commercial protocol. The result of DNA isolation stored at -20oC until amplificationanalysis. DNA isolation was successfully done, for further individual genetic ID building.
Comparative Cytogenetic Study on Male and Female Captive Sumatran Elephant in Elephant Training Center, Way Kambas National Park Priyambodo Priyambodo; Elly L. Rustiati
The International Journal of Tropical Veterinary and Biomedical Research Vol 3, No 1 (2018): Vol. 3 (1) May 2018
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.059 KB) | DOI: 10.21157/ijtvbr.v3i1.11333

Abstract

Sumatran elephant (Elephas maximus sumatranus) was one of five big mammals in Way Kambas National Park (WKNP). Cytogenetic study was a part of conservation effort for sumatran elephant, especially in conservation genetic field. Captive sumatran elephant’s blood in Elephant Training Center (ETC) WKNP was collected to karyotype analysis. Captive sumatran elephant’s chromosome was analyzed by squashing technique with pretreating cells in a hypotonic solution. The study of karyotypes were made by giemsa staining applied to captive elephant’s blood cell. The diploid number of captive sumatran elephant was 56 both in male and female. The karyotype analysis performed different fundamental number in male and female, both of total fundamental number (FN) and autosomal fundamental number (FNa). Chromosome of female sumatran elephant showed the FN = 8, and FNa = 6, but in male sumatran elephant FN = 7, and FNa = . The karyotypes of X chromosome was large submetacentric and Y chromosome was small acrocentric.. The karyotype presented here will compilled with DNA fingerprinting analysis of all captive sumatran elephant in ETC WKNP as references in future breeding policy.
Amplification of The GAPDH Gene from The Urine eDNA of Sumatran Rhino in Sumatran Rhino Sanctuary, Way Kambas National Park Priyambodo Priyambodo; Chicka Refina P. Putri; Elly L. Rustiati; Yeyen Kurniawati; Danisworo Zulkarnain; Dian Neli Pratiwi; Zulfi Arsan; Giyono Giyono; Ganis Mustikawati; Vindo R. Pertiwi; Sukatmoko Sukatmoko; Eko A. Srihanto; Enny Saswiyanti
Jurnal Biologi Tropis Vol. 23 No. 3 (2023): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i3.4989

Abstract

Profiling the genetic character of each individual sumatran rhino is important in order to maintain individual viability and genetic variability of Sumatran rhinos. Non-invasive sampling is needed to minimize the disturbance of individual sumatran rhinos, this is due to the solitary character of sumatran rhinos and tend to be afraid to interact with humans. Until now, non-invasive sampling of sumatran rhinos in Way Kambas National Park (WKNP) has only been done through the source of the puddle. This study aims to amplify the GAPDH gene from sumatran rhino environmental DNA (eDNA) sourced from urine. Urine sampling was carried out on four of the eight sumatran rhino individuals in WKNP. DNA extraction of four sumatran rhino urine samples was carried out with reference to the DNeasy® Blood & tissue kit extraction protocol. Amplification of eDNA extraction results was carried out by polymerase chain reaction (PCR) using GAPDH primers. Visualization of extraction and amplification results from four individual sumatran rhinos at TNWK was tested qualitatively with 1% agarose gel electrophoresis. Electrophoresis results showed positive results after amplification of four individual sumatran rhino urine samples at sizes between 100-200bp. This study successfully amplified the GAPDH gene from four sumatran rhino individuals in WKNP based on qualitative tests. In further conservation efforts, it is necessary to explore eDNA extraction from other potential sources.
Optimizing honey production in stingless bee farming P. Priyambodo; Elly L. Rustiati; Nindy Permatasari; Mahfud Sidik; Indah A. Lestari; Ani A. Yani; Lousanja D. Sa'uddah
Journal of Community Service and Empowerment Vol. 4 No. 2 (2023): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jcse.v4i2.26431

Abstract

Harapan Jaya Village, Way Ratai Subdistrict, Pesawaran Regency has very promising potential for cultivating trigona bees. Lecture and student from University of Lampung team conducted training on stingless bee cultivation in this village in 2022. At the end of 2022, several stingless bee colonies experienced deaths that were allegedly due to lack of nectar sources. This community service activity was carried out to provide understanding and training on vegetation management and adding stingless bee colonies. Activities are carried out with lectures, discussions, and hands-on practice. The results of the activities that have been carried out include the addition of seven types of nectar source plants and two stingless bee colonies. In addition, the community's understanding of the maintenance of stingless bee colonies and their supporting vegetation also increased.  This is very supportive of long-term programmed in the achievement of SDGs, especially in the goals of life on land and no poverty.
Digital Dissemination of Waste Management Smart Building as a Sustainable Solution for Waste Management: Sosialisasi Digital Gagasan Waste Management Smart Building sebagai Solusi Pengelolaan Limbah Berkelanjutan Priyambodo; Indah Ayu Lestari; Lisa Alfina Damayanti; Fahrul Azzami; Faiz Zainul Muttaqin; Fathan Naufal Ahsan; Hestin Oktiani
Mattawang: Jurnal Pengabdian Masyarakat Vol. 4 No. 3 (2023)
Publisher : Yayasan Ahmar Cendekia Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35877/454RI.mattawang1913

Abstract

Waste management in Indonesia is one of the problems that cannot be handled optimally. Waste Management Smart Building (WMSB) is an integrated waste management idea, both in the form of plastic waste and agricultural waste based on a zero waste system. Digital dissemination carried out through Unila TV as an effort to overcome integrated waste management. The stages of implementation carried out include; pre-production, production and post-production. The achievement indicator is seen from the percentage of likes and the percentage of positive comments from viewers. Data analysis is presented in graphical form and analyzed descriptively. Based on the analysis obtained from the video that broadcasted on Unila TV starting June 22, 2023, it has currently been watched 398 times (19.8%). There were 134 viewers who gave reactions in the form of likes (33.66%) and no viewers who gave dislike reactions (0%). Based on the comments given by the audience, 100% are positive which is expected to be a cognitive response that arises after an understanding regarding the information conveyed through the video.
ONLINE AND SEMI-ONLINE MENTORING METHODS: COMMUNITY EMPOWERMENT STRATEGIES DURING THE NEW NORMAL PANDEMIC Elly Lestari Rustiati; Elsa Virnarenata; Priyambodo Priyambodo; Salih Alimudin; Dwi Asmi; Edi Santoso; Yanti Yulianti; Dian Neli Pratiwi; Erdi Suroso
Jurnal Abdisci Vol 1 No 5 (2024): Vol 1 No 5 Tahun 2024
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/abdisci.v1i5.207

Abstract

 Community stewardship can be done directly. Direct interaction is commonly applied, appreciated, and easy to communicate. During the COVID-19 pandemic, direct interaction and visits are almost impossible due to activity limitations and social distancing. Online and semi-online interaction can be applied by inviting community limited representatives combined with the online method. Initiation on online communication is introduced. This project was conducted in companionship and training in Braja Harjosari and Labuhan Ratu VII, East Lampung. Following the introduction of the online and semionline methods, local people can apply and share their knowledge of the new normal paradigm.
KARAKTERISASI BEEBREAD DARI TUJUH SPESIES LEBAH TANPA SENGAT (STINGLESS BEE) BERDASARKAN KEANEKARAGAMAN POLLEN YANG DIKUMPULKANNYA Mahfud Sidik; Lousanja Dira Sa’uddah; Indah Ayu Lestari; Ani Andri Yani; Priyambodo Priyambodo
BIO-SAINS : Jurnal Ilmiah Biologi Vol 2 No 1 (2022): Bio-Sains Vol 2 No 1, Agustus 2022
Publisher : Fakultas Sains dan Teknologi, Universitas Islam As-Syafi'iyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6084/m9.figshare.23564679

Abstract

Abstrak Lebah tanpa sengat atau stinglessbee akhir-akhir ini seringkali dipromosikan sebagai hewan budidaya yang sangat menjanjikan. Akan tetapi masih sangat sedikit literatur yang dapat digunakan dalam pembudidayaan lebah ini. Penelitian ini bertujuan untuk mengetahui karakteristik Beebread yang dikumpulkan oleh 7 spesies stinglessbee. penelitian ini dilaksanakan pada bulan Februari – Mei 2022, sampel beebread diambil langsung dari sarang lebah di PT Suhita Lebah Indonesia kota Bandarlampung dan dari pembudidaya lebah yang ada di desa Harapan Jaya kecamatan Wayratai kabupaten Pesawaran. Sampel dianalisis dengan metode asetolisis dan diamati menggunakan mikroskop serta dikelompokkan berdasarkan ketentuan Wingenroth (2001). Dari pengamatan yang dilakukan diketahui bahwa Spesies Tetrigona apikalis, Tetrigona vidua, dan Tetragonula reepeni memiliki karakteristik beebread monoflora dengan pollen didominasi oleh tumbuhan Cocos nucifera. beebread Tetragonula testaceitarsis dan Tetragonula levicep berkarakter biflora, serta beebread Heterotrigona itama dan Geniotrigona thoracica berkarakter multiflora. Dari ketujuh spesies lebah yang diamati mengumpulkan pollen dari tumbuhan C.nucifera dan memiliki persentase yang tinggi. Pollen C.nucifera dalam jumlah yang signifikan disebabkan oleh preferensi Stinglessbee untuk mencari makan pada bunga dengan konsentrasi gula tinggi yang tersedia sepanjang tahun. Kata Kunci: Beebread, Geniotrigona, Heterotrigona, Stinglessbee, Tetragonula, Tetrigona. Abstract The stingless bee or stinglessbee has recently been promoted as a very promising farm animal. However, there is still very little literature that can be used in the cultivation of these bees. . This study aims to determine the characteristics of beebread collected by 7 species of stinglessbee. This research was conducted in February – May 2022, the beebread samples were taken directly from bee hives at PT Suhita Lebah Indonesia, Bandarlampung city and from bee cultivators in Harapan Jaya village, Wayratai district, Pesawaran district. Samples were analyzed by acetolysis method and observed using a microscope and grouped according to the provisions of Wingenroth (2001). From the observations made, it is known that the species Tetrigona apikalis, Tetrigona vidua, and Tetragonula reepeni have the characteristics of beebread monoflora with pollen dominated by Cocos nucifera plants. Beebreads Tetragonula testaceitarsis and Tetragonula levicep have biflora characteristics, and Heterotrigona itama and Geniotrigona thoracica beebreads have multiflora characteristics. Of the seven species of bees observed, they collected pollen from the C. nucifera plant and had a high percentage. The significant amount of C. nucifera pollen was due to the Stinglessbee's preference for foraging on flowers with high sugar concentrations available throughout the year. Keywords : Beebread, Geniotrigona, Heterotrigona, Stinglessbee, Tetragonula, Tetrigona.
Pemanfaatan Botol Bekas untuk Vertical Garden sebagai Upaya Pencapaian Kampus Berkelanjutan Berbasis SDGs di Fakultas MIPA Universitas Lampung Priyambodo Priyambodo; Aspita Laila; John Hendri; Rinawati Rinawati; Heri Satria
Jurnal Pengabdian Dharma Wacana Vol 4, No 3 (2023): Jurnal Pengabdian Dharma Wacana
Publisher : Yayasan Pendidikan dan Kebudayaan Dharma Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37295/jpdw.v4i3.476

Abstract

Universitas Lampung (Unila) terus berupaya menjadi salah satu kampus berkelanjutan terbaik di Indonesia. Oleh karena itu, seluruh elemen berupaya untuk mewujudkan butir-butir Sustainable Development Goals (SDGs), termasuk Fakultas MIPA. Salah satu butir yang dapat diwujudkan adalah butir life on land dengan mengupayakan langkah-langkah strategis untuk mengurangi kehilangan keanekaragaman hayati. Beberapa upaya telah dilaksanakan, termasuk penambahan mata kuliah berwawasan lingkungan, pelaksanaan green kurban, pembuatan biopori, dan pengolahan limbah organik. Pengabdian kepada Masyarakat ini dilaksanakan untuk memanfaatkan limbah anorganik berupa botol bekas untuk dijadikan sebagai wahana tanam pada vertical garden. Pelaksanaan pengabdian melibatkan masyarakat akademik Fakultas MIPA Unila. Pada akhir kegiatan tercipta sebuah vertical garden yang ditanami dengan tanaman hias untuk memberikan nilai estetika dan menambah nyaman serta menjadi pengendali atas iklim mikro di sekitar penanaman.
Co-Authors Agustin, Meli Agustrina, Rochmah Ahyul Heni Alvin Wiwiet Susanto Alvin Wiwiet Susanto Amaliya Sabana Ani A. Yani Ani Andri Yani Ani Andri Yani Aprilia Eka Putri Arsan, Zulfi Aspita Laila Ayuni, Ubaid Jan Bambang Irawan Bondan Pergola Chicka Refina P. Putri Danisworo Zulkarnain DEDI CANDRA Diah E. Anggraini Diah Esti Anggraini Dian Neli Pratiwi Dian Neli Pratiwi Dian Neli Pratiwi Dian Neli Pratiwi Dwi Asmi Dwi Ayu Febriyani Edi Santoso Eko A. Srihanto Eko Agus Prihanto Eko Agus Srihanto Eko Agus Srihanto Eko Agus Srihanto, Eko Agus Elfita Nova Yunior Elizabeth D. Krismuniarti Elly L. Rustiati Elly L. Rustiati Elly L. Rustiati Elly Lestari Rusitati Elly Lestari Rustiati Elly Lestari Rustiati Elsa Virnarenata Emantis Rosa Enny Saswiyanti Enny Saswiyanti Enny Saswiyanti Erdi Suroso Erdi Suroso Eti Ernawiati Eti Ernawiati Eva Yunita Fahrul Azzami Faiz Zainul Muttaqin Fathan Naufal Ahsan Favorisen R. Lumbanraja Firwantoni Firwantoni Ganis Mustikawati Gina Dania Pratami, Gina Dania Giyono Giyono Giyono Giyono Heri Satria Hestin Oktiani I Gede Swibawa I Wayan Tony Candra Ibransyah, Rasyid Indah A. Lestari Indah Ayu Lestari Indah Ayu Lestari Intan Diani Fardinatri Irwan Nasution Jani Master Jani Master, Jani John Hendri Junaidi Junaidi Krismuniarti, Elisabeth Devi Kurniawati, Yeyen Lestari, Septi Wahyu Lili Chrisnawati Lisa Alfina Damayanti Liza Angeliya Lousanja D. Sa'uddah Lousanja Dira Sa’uddah Lousanja Dira Sa’uddah Lu&#039;lu&#039; Kholidah Fauziah Lu&#039;lu&#039; Kholidah Fauziah M. Kanedi Mahfud Sidik Mahfud Sidik Mahfud Sidik Martha Lulus Lande Masniar Vina Kesti Melya Riniarti Mustikawati, Ganis Nindy Permatasari Novianasari, Tika Nugraha, M. Aqwam Nuning Nurcahyani Patrice Kevin Marcus Pertiwi, Vindo R. Pratiwi, Dian N. Pratiwi, Dian Neli Putri Ayu Febrina Putri, Chicka R.P. Raden Fadly Bayu Dwiyoga Resti Puspa Kartika Sari Resti Puspa Kartika Sari Rinawati Rinawati Rochmah Agustrina Rochmah Agustrina Rodliyah, Aini Robby Romaya Wulan Suciningtyas Rustiati, Elly L. Salih Alimudin Sandra, Shifa Saswiyanti, Enny Sekar Pratiwi Sevira Nur Azmi Siti Asiyah Srihanto, Eko A. Sukatmoko Sukatmoko Sukatmoko, Sukatmoko Sunandar Susanto, Alvin Wiwiet Susanto, Gregorius Nugroho Thesalonika, Natasya Tristiyanto Tristiyanto Tugiyono Tugiyono Tugiyono Tugiyono Tugiyono Vidya Kurniasih Viki Ramadan Vindo R. Pertiwi Virnarenata, Elsa Warsono Warsono Waryoko Susandi Winarno Winarno Winda Rahmawati Yanti Yulianti Yanti Yulianti Yeyen Kurniawati Yuni Tinasari Zulfi Arsan Zulkarnain, Danisworo