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Supporting Environmental Resilience through Biological Approach: Initial Step for Building Robusta Coffee Germplasm Database Rustiati, Elly Lestari; Priyambodo, Priyambodo; Pratiwi, Dian Neli; Srihanto, Eko Agus; Lestari, Septi Wahyu; Sandra, Shifa; Winarno, Winarno; Thesalonika, Natasya
Bioma : Berkala Ilmiah Biologi Vol. 26 No 2, Tahun 2024
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2024.65548

Abstract

In response to environmental challenges and the critical imperative for sustainable progress, rural regions including agriculture practices are important in ecological robustness. It can be achieved through a synergistic relationship between rural development sustainability and the biological sciences. Concurrently, research in stewardship approaches are underway to amass data on robusta coffee, Coffea canephora, species. In collaboration with Lampung Disease Investigation Centre and local coffee farmers in Pesawaran, Lampung, and under DIPA BLU Universitas Lampung year 2024, initial ongoing work on coffee germ plasm data is done. Robusta coffee leaves were sampled from 2 blocks of traditional coffee plantations, Wiyono and Bogorejo, Pesawaran, Lampung.  Of 18 robusta leaves’ samples DNA extraction, electrophoresis showed 14 samples with good results. The optimum temperature for amplification is 62 °C and 63 °C.  The results will be sequenced for its species confirmation.  This project is expected to contribute bottom up towards inclusive and sustainable rural futures at the local level.
Efektivitas Ekoenzim Kulit Pisang Kepok Manado Muda sebagai Insektisida Nabati terhadap Hama Penghisap Buah Kakao (Helopeltis sp.) Agustin, Meli; Pratami, Gina Dania; Priyambodo; Agustrina, Rochmah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2025): February 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i1.9288

Abstract

Kakao merupakan tanaman perkebunan yang mempunyai peran cukup penting dalam perekonomian nasional. Salah satu kendala pada budidaya kakao adalah kehadiran organisme pengganggu tanaman, yaitu kepik penghisap buah kakao (Helopeltis sp.). Pemakaian insektisida kimia secara berlebihan dapat menimbulkan dampak negatif sehingga diperlukan insektisida alternatif yang ramah lingkungan. Ekoenzim kulit pisang kepok manado muda memiliki kandungan bahan aktif yang berpotensi dijadikan insektisida nabati. Penelitian ini bertujuan untuk mengetahui konsentrasi ekoenzim kulit pisang kepok manado muda yang efektif dalam mematikan hama penghisap buah kakao. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) faktorial 2 faktor perlakuan dan 3 ulangan. Faktor pertama adalah konsentrasi ekoenzim (0%, 5%, 10%, 15%, dan 20%). Faktor kedua adalah waktu pengamatan (6, 12, 24, 48, dan 72 jam). Data hasil pengamatan dianalisis menggunakan ANOVA dengan uji lanjut Tukey dan nilai LC50 dianalisis menggunakan probit. Hasil penelitian menunjukkan bahwa ekoenzim kulit pisang kepok manado muda berpotensi sebagai insektisida nabati terhadap hama penghisap buah kakao dengan konsentrasi paling efektif adalah 20%. Sementara hasil analisis probit didapatkan nilai LC50 sebesar 17,95% pada waktu pengamatan 72 jam.
STUDI FENOMENA GENETIKA POPULASI MELALUI ANALISIS PROPORSI GOLONGAN DARAH MAHASISWA SARJANA DAN DIPLOMA FAKULTAS MIPA UNILA BERDASARKAN SISTEM ABO Nugraha, M. Aqwam; Priyambodo, Priyambodo
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5624

Abstract

Pengetahuan atas sistem penggolongan darah ABO sangat penting sebagai upaya pengenalan diri lebih komprehensif dan bentuk upaya antisipatif dalam kondisi darurat medis. Penelitian ini dilakukan untuk mengetahui proporsi golongan darah dan frekuensi alel mahasiswa sarjana dan diploma FMIPA Unila berdasarkan sistem ABO. Penelitian telah dilakukan dengan penyebaran kuesioner dan informed consent secara daring. Dari 3.244 total mahasiswa, didapatkan informasi bahwa 384 mahasiswa telah mengetahui golongan darahnya. Proporsi tertinggi golongan darah mahasiswa sarjana dan diploma FMIPA Unila adalah O dengan persentase 36,72%, diikuti dengan golongan darah B (28,13%), golongan darah A (25,52%) dan golongan darah AB (9,64%). Berdasarkan formulasi perhitungan Hukum Hardy-Weinberg, diketahui bahwa frekuensi alel A, B, dan O berturut-turut adalah 0,18; 0,21; dan 0,61.
ONLINE AND SEMI-ONLINE MENTORING METHODS: COMMUNITY EMPOWERMENT STRATEGIES DURING THE NEW NORMAL PANDEMIC Elly Lestari Rustiati; Elsa Virnarenata; Priyambodo Priyambodo; Salih Alimudin; Dwi Asmi; Edi Santoso; Yanti Yulianti; Dian Neli Pratiwi; Erdi Suroso
Jurnal Abdisci Vol 1 No 5 (2024): Vol 1 No 5 Tahun 2024
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/abdisci.v1i5.207

Abstract

 Community stewardship can be done directly. Direct interaction is commonly applied, appreciated, and easy to communicate. During the COVID-19 pandemic, direct interaction and visits are almost impossible due to activity limitations and social distancing. Online and semi-online interaction can be applied by inviting community limited representatives combined with the online method. Initiation on online communication is introduced. This project was conducted in companionship and training in Braja Harjosari and Labuhan Ratu VII, East Lampung. Following the introduction of the online and semionline methods, local people can apply and share their knowledge of the new normal paradigm.
COMPANIONSHIP ON EDUCATION AND INTRODUCTION TO BAT SPECIES IN BRAJA HARJOSARI AND LABUHAN RATU VII, EAST LAMPUNG Elly Lestari Rustiati; Dian Neli Pratiwi; Enny Saswiyanti; Vidya Kurniasih; Sevira Nur Azmi; Elfita Nova Yunior; Raden Fadly Bayu Dwiyoga; Priyambodo; Eko Agus Prihanto; Ahyul Heni; Dwi Ayu Febriyani; Waryoko Susandi; I Wayan Tony Candra; Sunandar
Jurnal Abdisci Vol 1 No 12 (2024): Vol 1 No 12 Tahun 2024
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/abdisci.v1i12.488

Abstract

Under the HETI Project Program of Universitas Lampung 2023/2024, education and introduction to bats have been conducted in Braja Harjosari and Labuhan Ratu VII, East Lampung. Knowledge about bats and their ecological roles is essential as a conservation effort, particularly in residential areas adjacent to natural bat habitats. Information on bats, including their natural habitats, behaviors, and roles in supporting human life, as well as general species identification, was provided. The selection of locations, mist net installation, capture, handling, and release were demonstrated, including oral swab sample collection. The community’s response and enthusiasm were very positive in understanding bats as wildlife and their roles in human life.
Uji Biolarvasida Ekstrak Metanol Daun Pepaya Jepang pada Larva Anopheles sp. Dea Farrah Khairunnisa; Emantis Rosa; Priyambodo; Hendri Busman
OKTAL : Jurnal Ilmu Komputer dan Sains Vol 5 No 05 (2026): OKTAL : Jurnal Ilmu Komputer Dan Sains
Publisher : CV. Multi Kreasi Media

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Malaria remains a major health problem in endemic areas such as Pesawaran Regency, Lampung, transmitted through the bites of infected female Anopheles mosquitoes. Continuous use of synthetic larvicides may lead to resistance, toxicity to non-target organisms, and environmental pollution, thus safer biolarvicidal alternatives are needed. Japanese papaya leaves (Cnidoscolus aconitifolius) are known to contain secondary metabolites with potential biolarvicidal activity. This study aimed to determine the biolarvicidal activity of methanol extract of Japanese papaya leaves against the mortality of Anopheles larvae and to observe morphological changes in larvae after exposure. The study used a Completely Randomized Design with five treatments: 0% (control), 0.5%, 1%, 1.5%, and 2%, each with five replications. Data were analyzed using one-way ANOVA followed by the Least Significant Difference (LSD) test. Phytochemical screening revealed the presence of alkaloids, flavonoids, saponins, tannins, phenols, and steroids. ANOVA results showed significant differences among treatments (p<0.001), while the LSD test indicated that each concentration had a significantly different effect on larval mortality (p<0.05). The highest mortality was observed at 2% concentration, reaching 82.5% at 24 hours and 100% at 48 hours. The methanol extract also caused morphological damage in larvae, including tissue lysis, digestive tract damage, and respiratory system impairment. It can be concluded that the methanol extract of japanese papaya leaves has potential as a natural biolarvicide against Anopheles larvae.
EDUKASI DAN PELATIHAN BUDIKDAMBER SEBAGAI UPAYA PENINGKATAN KETAHANAN PANGAN RUMAH TANGGA DI KELURAHAN SEPANG JAYA Firsto Berianto; Nazila Hafidza; Fauzia Farsya Syuadi; Erni Sapitri; Muhakmal; M. Raihan Wicak Setiadi; Galih Abie Sadewa; Putri Handayani; Bunga Ayuningtias; Nailah Khairunnisa; Muhammad Arzuqo Bilhad; Alya Fadiyah Rahmah; Zahra Asnifa Setya Putri; Agung Kusuma Wijaya; Deny Sapto Chondro Utomo; Veronica Wanniatie; Priyambodo; Sofyan Musyabiq Wijaya
Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Vol. 5 No. 1 (2026): Jurnal Pengabdian Kepada Masyarakat FP Unila, Edisi Maret 2026
Publisher : Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpfp.v5i1.12752

Abstract

The issue of household food security in urban areas is a major concern, especially given limited land availability and the growing need for nutritious food. The Sepang Jaya neighborhood faces similar challenges, necessitating innovative solutions that are easy for the community to implement. This community service project focuses on implementing fish farming in buckets (budikdamber) as a strategy to improve household food security by utilizing limited space. The objective of this project is to enhance the community’s knowledge, skills, and self-reliance in independently producing animal and plant-based protein sources through the budikdamber system. The methods employed included a participatory approach involving the delivery of educational materials on the concepts, benefits, advantages, and techniques of budikdamber, as well as hands-on training in preparing the aquaculture medium and caring for the fish. Implementation strategies utilized interactive sessions, discussions, live demonstrations, and on-site guidance. The results of the activity demonstrated an increase in the community’s understanding of budikdamber techniques, as well as a growing interest in implementing this system in their own homes. Community members were able to assemble the aquaculture media independently and understand the process from maintenance through harvest. Overall, this activity contributed to strengthening household food security by providing a sustainable source of nutritious food, while also promoting community empowerment based on local potential.
Mitochondrial 16S rRNA Characterization of Heterotrigona itama Colonies from Central Lampung, Indonesia Priyambodo Priyambodo; Aditya Fahrezi; Elly Lestari Rustiati; Enny Saswiyanti; Eko Agus Srihanto; Aulia Imtitsal; Dian Neli Pratiwi; Nindy Permatasari
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1301-1307

Abstract

Stingless bees play important ecological and economic roles in tropical ecosystems, yet molecular baseline data for many regions in Sumatra remain limited. This study aimed to characterize mitochondrial genetic variation of Heterotrigona itama colonies from Punggur Subdistrict, Central Lampung, Indonesia, using partial 16S rRNA sequences. Three managed colonies were analyzed through PCR amplification and Sanger sequencing, followed by sequence alignment, BLAST comparison, genetic distance estimation using the Kimura-2-Parameter model, and phylogenetic reconstruction. All samples produced consistent amplicons of approximately 500 bp and showed complete nucleotide identity, indicating a single mitochondrial haplotype among the analyzed colonies. BLAST results revealed high similarity (96.91–98.38%) with reference sequences of H. itama. Genetic distance analysis showed zero divergence among the sampled colonies and low divergence (0.0069–0.0182) relative to other H. itama sequences, while higher divergence was observed with related taxa. Phylogenetic analysis placed all samples within a well-supported H. itama clade. These findings indicate mitochondrial uniformity among the sampled colonies and provide a preliminary genetic baseline for H. itama in Central Lampung. However, broader conclusions on population structure require further studies with larger sample sizes and additional molecular markers.
Genetic Research Trends in Caffeine Biosynthesis of Robusta Coffee (Coffea canephora) Nindy Permatasari; Sismita Sari; Sri Nurmayanti; Lu'lu' Kholidah Fauziah; Resti Puspa Kartika Sari; Maisuri Hardani; Siti Hamidatul Aliyah; Priyambodo Priyambodo
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11738

Abstract

Research on caffeine biosynthesis in Coffea canephora has expanded across multiple disciplines, yet its global genetic research trends remain insufficiently mapped. This study aimed to analyze the development and thematic structure of genetic research on caffeine biosynthesis in C. canephora using a bibliometric approach. Data were retrieved from the Scopus database through keyword-based sampling and filtered based on relevance to genetic and biosynthetic aspects. Bibliometric analyses were conducted to examine publication trends, authorship patterns, country contributions, and keyword co-occurrence networks. The results show a significant increase in publications since the early 2000s, with dominant contributions from the United States, Brazil, and China. Keyword mapping reveals four major research clusters, including plant metabolism, clinical and nutritional studies, experimental models, and genetic variability. However, studies explicitly focusing on the genetic regulation of caffeine biosynthesis, particularly involving N-methyltransferase genes, remain relatively limited compared to broader multidisciplinary themes.These findings indicate that caffeine research in C. canephora is highly interdisciplinary but still lacks a strong emphasis on genetic mechanisms, highlighting the need for more targeted molecular and genomic studies to support coffee improvement and functional trait development.
Co-Authors Aditya Fahrezi Agung Kusuma Wijaya Agustin, Meli Agustrina, Rochmah Ahyul Heni Alvin Wiwiet Susanto Alvin Wiwiet Susanto Alya Fadiyah Rahmah Amaliya Sabana Ani A. Yani Ani Andri Yani Ani Andri Yani Aprilia Eka Putri Arsan, Zulfi Aulia Imtitsal Ayuni, Ubaid Jan Bambang Irawan Bondan Pergola Bunga Ayuningtias Chicka Refina P. Putri Danisworo Zulkarnain Dea Farrah Khairunnisa DEDI CANDRA Deny Sapto Chondro Utomo Diah E. Anggraini Diah Esti Anggraini Dian Neli Pratiwi Dian Neli Pratiwi Dian Neli Pratiwi Dian Neli Pratiwi Dian Neli Pratiwi Dwi Asmi Dwi Ayu Febriyani Edi Santoso Eko A. Srihanto Eko Agus Prihanto Eko Agus Srihanto Eko Agus Srihanto Eko Agus Srihanto Eko Agus Srihanto, Eko Agus Elfita Nova Yunior Elizabeth D. Krismuniarti Elly L. Rustiati Elly L. Rustiati Elly L. Rustiati Elly Lestari Rusitati Elly Lestari Rustiati Elly Lestari Rustiati Elsa Virnarenata Emantis Rosa Emantis Rosa Enny Saswiyanti Enny Saswiyanti Enny Saswiyanti Enny Saswiyanti Erdi Suroso Erdi Suroso Erni Sapitri Eti Ernawiati Eti Ernawiati Eva Yunita Fauzia Farsya Syuadi Firsto Berianto Firwantoni Firwantoni Galih Abie Sadewa Ganis Mustikawati Gina Dania Pratami, Gina Dania Giyono Giyono Giyono Giyono Hendri Busman I Gede Swibawa I Wayan Tony Candra Ibransyah, Rasyid Indah A. Lestari Indah Ayu Lestari Indah Ayu Lestari Intan Diani Fardinatri Irwan Nasution Jani Master Jani Master, Jani Junaidi Junaidi Krismuniarti, Elisabeth Devi Kurniawati, Yeyen Lestari, Septi Wahyu Lili Chrisnawati Liza Angeliya Lousanja D. Sa'uddah Lousanja Dira Sa’uddah Lousanja Dira Sa’uddah Lu&#039;lu&#039; Kholidah Fauziah Lu'lu' Kholidah Fauziah Lumbanraja, Favorisen R M. Raihan Wicak Setiadi Mahfud Sidik Mahfud Sidik Mahfud Sidik Maisuri Hardani Martha Lulus Lande Masniar Vina Kesti Melya Riniarti Muhakmal Muhammad Arzuqo Bilhad Mustikawati, Ganis Nailah Khairunnisa Nazila Hafidza Nindy Permatasari Nindy Permatasari Novianasari, Tika Nugraha, M. Aqwam Nuning Nurcahyani Patrice Kevin Marcus Pertiwi, Vindo R. Pratiwi, Dian N. Pratiwi, Dian Neli Putri Ayu Febrina Putri Handayani Putri, Chicka R.P. Raden Fadly Bayu Dwiyoga Resti Puspa Kartika Sari Rochmah Agustrina Rochmah Agustrina Rodliyah, Aini Robby Romaya Wulan Suciningtyas Rustiati, Elly L. Salih Alimudin Sandra, Shifa Saswiyanti, Enny Sekar Pratiwi Sevira Nur Azmi Sismita Sari Siti Asiyah Siti Hamidatul Aliyah Sofyan Musyabiq Wijaya Sri Nurmayanti Srihanto, Eko A. Sukatmoko Sukatmoko Sukatmoko, Sukatmoko Sunandar Susanto, Alvin Wiwiet Susanto, Gregorius Nugroho Thesalonika, Natasya Tristiyanto Tristiyanto Tugiyono Tugiyono Tugiyono Tugiyono Veronica Wanniatie Vidya Kurniasih Vindo R. Pertiwi Virnarenata, Elsa Warsono Warsono Waryoko Susandi Winarno Winarno Winda Rahmawati Yanti Yulianti Yanti Yulianti Yeyen Kurniawati Yuni Tinasari Zahra Asnifa Setya Putri Zulfi Arsan Zulkarnain, Danisworo