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Jaswandi .
Fakultas Peternakan, Universitas Andalas
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Journal : Tropical Animal Science Journal

 1 
Impacts of Cryopreservation on Semen Quality and Sperm Protein Profiles of Pesisir Bulls Ananda; Gusdinal, H.; Ramadhan, R.; Abimanyu, A. A.; Ningsih, W. H.; Jaswandi
Tropical Animal Science Journal Vol. 48 No. 3 (2025): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2025.48.3.189

Abstract

This study aimed to evaluate the impacts of cryopreservation on semen quality and sperm protein profile in Pesisir bulls. Semen samples were collected from three bulls and analyzed in fresh and post-thaw conditions. The sperm motility and kinematic variables were assessed using Computer-Assisted Sperm Analysis (CASA), while sperm viability and plasma membrane integrity (PMI) were evaluated through eosin-nigrosin staining and the hypoosmotic swelling test (HOST), respectively. Subsequently, the total protein concentration (PC) and profile were examined using SDS-PAGE. The results showed that there was a significant decrease in semen quality after thawing, with sperm motility reducing from 81.10% to 70.22%, viability reducing fom 87.47% to 77.27%, and PMI reducing from 85.09% to 71.32% (p<0.05). Kinematic variables such as velocity, straightness, and beat cross frequency also decreased significantly. Protein analysis showed a reduction in total concentration from 1.78 mg/mL to 1.19 mg/mL and alterations in protein band distribution, with the loss of specific high- and low-molecular weight after freezing. These results suggested that cryopreservation negatively impacts semen quality and sperm protein integrity, potentially impairing fertility. Moreover, further studies were recommended to optimize cryopreservation protocols and mitigate adverse effects.
Co-Authors A Boediono A. Agustar Abdul Rahman Abimanyu, A. A. Adidin Aidin Maulana Adisti Rastosari Aditya Alqamal Alianta Aida Astiti Aji, Riyan Nugroho Alfian Asri Amrizal Anas Ananda Ananda Ananda Ananda, Ananda Ananda, Pinta Aftaprilia Rizki Anna Farhana Anna Farhana Anna Farhana Aprisal Aprisal, Aprisal arfai Arif Rahmat Auli Two Putra Syahminan B.P Zespin B.P. Zesfin Baehaqi Basyar, Basril C. Arman D. Dianti D. Mardona Damayanti, Elok Dara Surtina Defrinaldi Defrinaldi Delmita Nugrah Wati Delsi Afrini Dwi Yuzaria E. Nurdin E. Purwati Edi Set Ediset, Ediset Elfajri, Rifqi Elly Roza Elvina Endang Purwati RN Esti Rahayu F. Afrianti F. Arlina F. L. Syaiful Faizal, Noval Jumatul FAJAR HIDAYAT Faqih, M Ferawati, . Ferdinal Rahim Ferry Lismanto Syaiful Firdaus Gusdinal, H. H. Purwanto Hafrijal Syandri Harif Gusdinal Haris Satria Harissatria, Harissatria Hendri . Hendri Hendri Hendri Hendri Hendri Hendri Hendri Hendri Hendri Hendri Heriyanto, Edwin Husmaini Husmaini Husmaini, Husmaini I Gde Eka Budhiyadnya Indri Juliyarsi Indriastuti, Rhesti J. Hendri James Hellyward Jhon Hendri Jhon Hendri John Hendri John Hendri Khasrad . Leonardo E Lisna Sukmawati M A Setiadi M Ikhsan Rias M R Toelihere Mangku Mundana Mardhiyah, Indah Masrizal Masrizal Masrizal Masrizal Melati Trisnaneng Putri Mylaufa Asyraf Netti Aryani Ningsih, W. H. Putri Oktavially Putri, Atika Dwi Putri, Sri Mardhiani Penta R Saladin R. Saladin Ramadhan, R. Reswati, Reswati Rohmatulloh, Pradekita Rusdimansyah Rusdimansyah Rusfidra rusfidra Rusmana Rusmana S. Asmairicen S. Melia Sitepu, Sukma Aditya Siti Hanifah Sri Melia Tinda Afriani Windy Melinda Wulandari Wulandari Y Sukra Y. E. Satria Yendraliza - Yetmaneli, Yetmaneli Yuherman, . Yurnalis Yurnalis Yustiti, Aida Z Udin Z. Udin Z. Udin Z. Udin Zaituni Udin Zaituni Udin Zesfin BP Zuhelmi Zen Zumarni Zumarni