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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Buletin Peternakan Biospecies Jurnal Natur Indonesia Jurnal Perikanan dan Kelautan Jurnal Sain Peternakan Indonesia JURNAL PETERNAKAN Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture Jurnal Peternakan Indonesia Tropical Animal Science Journal JURNAL ILMIAH PETERNAKAN TERPADU JOURNAL OF SCIENCE AND SOCIAL RESEARCH Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat TELEKONTRAN: Jurnal Ilmiah Telekomunikasi, Kendali dan Elektronika Terapan Jurnal Hilirisasi IPTEKS (JHI) JCRS (Journal of Community Research and Service) Abdi Masyarakat Jurnal Triton Wahana Peternakan Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks JETE Jurnal Ilmiah Ilmu-Ilmu Peternakan Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia Jurnal Veteriner
Jaswandi .
Fakultas Peternakan, Universitas Andalas
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Journal : Buletin Peternakan

 1 
Effect of Extender and Cooling Rate on the Quality of Frozen Thawed Semen of Bali Bull (Bos Sondaicus) Anna Farhana; Zaituni Udin; Jaswandi Jaswandi; Riyan Nugroho Aji
Buletin Peternakan Vol 42, No 4 (2018): BULETIN PETERNAKAN VOL. 42 (4) NOVEMBER 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i4.35173

Abstract

The purpose of this study was to observe the effect of extender and cooling rate on the quality of frozen thawed semen of Bali bull (Bos sondaicus). The experiment was conducted at Laboratory of Animal Biotechnology, Faculty of Animal Husbandry, Andalas University. A completely randomized design of factorial 2 x 3, 2 different extenders and 3 cooling rates with 3 bulls as the replicate. Data were analyzed using analysis of variance (ANOVA). The first factors was extender such as tris citrate egg yolk (TCEY) and tris citrate soy milk (TCSM). The second factors was the cooling rate such as: 15ºC/min, 10ºC/min and 5ºC/min. Variables were: motility, viability, abnormality, and membrane integrity. The results of treatment of TCEY extender on the average of motility 42.67±5.17%, viability of 53.78±3.79%, abnormality of 18.89±1.07%, and membrane integrity of 35.44±3.01%. The effect of cooling rate of 10°C /min has the highest semen motility of 44.35±5.28%, viability of 57.17±1.18%, abnormality of 17.84 ± 0.23 % and membrane integrity of 36.83±2.12%. The interaction between extender and cooling rate was founded significant different (P<0.05). Otherwise of extender and cooling rate on post-thawed of motility, viability and membrane integrity, had no difference (P>0.05) on semen abnormality. It can be concluded that tris-citrate egg yolk (TCEY) extender with cooling rate of 10ºC / min were the best semen motility, viability, and membrane integrity of spermatozoa.
Sperm Longevity and Motility in Ringer’s Lactate Solution with Addition of Egg Yolk among Five Phenotypes of Kokok Balenggek Chicken Ananda Ananda; Jaswandi Jaswandi; Rusfidra Rusfidra; Harif Gusdinal
Buletin Peternakan Vol 47, No 3 (2023): BULETIN PETERNAKAN VOL. 47 (3) AUGUST 2023
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v47i3.83647

Abstract

The application of cryopreservation to preserve germplasm in such specific breed requires preliminary studies, primarily related to the resistance of spermatozoa to low temperatures (4 – 5 °C) as measured by their motility and longevity. In this study, semen taken from five phenotypes of Kokok Balengeek Chicken (KBC) (Biriang, Jalak, Kinantan, Kuriak, and Taduang) was used to evaluate the effect of Ringer’s Lactate-egg yolk diluent on longevity and motility of spermatozoa. The treatments consisted of Ringer’s Lactate (RL) solution added with egg yolk at a concentration of 1% (RLKT1), 3% (RLKT3), and 5% (RLKT5). Evaluation of fresh semen showed that the spermatozoa of Jalak had the highest motility, namely 75.63 ± 0.5% (P<0.05). Post-dilution longevity and motility observations were carried out at 0, 24, 48, and 72 h, significantly decreasing each time (P<0.05). The lowest range of reduction was found in Jalak spermatozoa diluted with RL with longevity of 7.75 ± 0.70 days. Overall, the RL diluent showed the highest motility after 24 h, namely 41.13 ± 2.27%. Adding egg yolks to Ringer's Lactate solution could not maintain the motility of KBC spermatozoa when stored at 4-5°C for 48-72 h.
Sperm Kinematics of Pesisir Bull Thawed at Different Temperatures and Times Hendri, Hendri; Jaswandi, Jaswandi; Indriastuti, Rhesti; Ananda, Ananda
Buletin Peternakan Vol 48, No 4 (2024): BULETIN PETERNAKAN VOL. 48 (4) NOVEMBER 2024
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v48i4.96459

Abstract

Pesisir bulls from West Sumatra, Indonesia, have the potential to be valuable meat producers due to their adaptability and unique characteristics, but reproductive challenges necessitate the use of artificial insemination (AI) technology. The success of AI relies on the quality of frozen semen, which is influenced by thawing conditions, making the evaluation of sperm kinematics through Computer-Assisted Sperm Analysis (CASA) crucial for a comprehensive assessment of sperm function. This research evaluated the kinematics of frozen semen spermatozoa from Pesisir bulls. Frozen semen samples (n = 20) were thawed at 37°C for 10, 20, and 30 s and at 25°C for 30, 45, and 60 s. Kinematic parameters were observed using a Computer-Assisted Sperm Analyzer (CASA), including total motility (M), progressive motility (PM), the velocity of the average path (VAP) (?m/sec), the velocity of curvilinear (VCL) (?m/sec), the velocity of straight line (VSL) (?m/sec), straightness (STR) (%), linearity (LIN) (%), wobble (WOB) (%), amplitude of lateral head displacement (ALH) (?m), and beat/cross-frequency (BCF) (Hz). Thawed frozen semen at a temperature of 37°C for 20 s significantly (p<0.05) increased M, PM, and ALH. Furthermore, thawed frozen semen at 37°C for all durations and at 25°C for 60 s showed better quality for VAP, VCL, and VSL (p<0.05). Meanwhile, thawed frozen semen at 25°C increased STR, LIN, and WOB. However, thawing temperature and duration had no significant effect on BCF. Correlation analysis conducted on semen thawed at a temperature of 37°C for 20 s found that VAP was correlated with VCL, VSL, and ALH, while semen thawed at a temperature of 25°C for 60 s found that VAP was correlated with VCL, VSL, STR, LIN, and ALH. Most kinematic parameters were significantly better in the thawing treatment at 37°C compared to 25°C, suggesting careful consideration 
Co-Authors A Boediono A. Agustar Abdul Rahman Abimanyu, A. A. Adidin Aidin Maulana Adisti Rastosari Aditya Alqamal Alianta Aida Astiti Aji, Riyan Nugroho Alfian Asri Amrizal Anas Ananda Ananda Ananda Ananda, Ananda Ananda, Pinta Aftaprilia Rizki Anna Farhana Anna Farhana Anna Farhana Aprisal Aprisal, Aprisal arfai Arif Rahmat Auli Two Putra Syahminan B.P Zespin B.P. Zesfin Baehaqi Basyar, Basril C. Arman D. Dianti D. Mardona Damayanti, Elok Dara Surtina Defrinaldi Defrinaldi Delmita Nugrah Wati Delsi Afrini Dwi Yuzaria E. Nurdin E. Purwati Edi Set Ediset, Ediset Elfajri, Rifqi Elly Roza Elvina Endang Purwati RN Esti Rahayu F. Afrianti F. Arlina F. L. Syaiful Faizal, Noval Jumatul FAJAR HIDAYAT Faqih, M Ferawati, . Ferdinal Rahim Ferry Lismanto Syaiful Firdaus Gusdinal, H. H. Purwanto Hafrijal Syandri Harif Gusdinal Haris Satria Harissatria, Harissatria Hendri . Hendri Hendri Hendri Hendri Hendri Hendri Hendri Hendri Hendri Hendri Heriyanto, Edwin Husmaini Husmaini Husmaini, Husmaini I Gde Eka Budhiyadnya Indri Juliyarsi Indriastuti, Rhesti J. Hendri James Hellyward Jhon Hendri Jhon Hendri John Hendri John Hendri Khasrad . Leonardo E Lisna Sukmawati M A Setiadi M Ikhsan Rias M R Toelihere Mangku Mundana Mardhiyah, Indah Masrizal Masrizal Masrizal Masrizal Melati Trisnaneng Putri Mylaufa Asyraf Netti Aryani Ningsih, W. H. Putri Oktavially Putri, Atika Dwi Putri, Sri Mardhiani Penta R Saladin R. Saladin Ramadhan, R. Reswati, Reswati Rohmatulloh, Pradekita Rusdimansyah Rusdimansyah Rusfidra rusfidra Rusmana Rusmana S. Asmairicen S. Melia Sitepu, Sukma Aditya Siti Hanifah Sri Melia Tinda Afriani Windy Melinda Wulandari Wulandari Y Sukra Y. E. Satria Yendraliza - Yetmaneli, Yetmaneli Yuherman, . Yurnalis Yurnalis Yustiti, Aida Z Udin Z. Udin Z. Udin Z. Udin Zaituni Udin Zaituni Udin Zesfin BP Zuhelmi Zen Zumarni Zumarni