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Journal : Biotropika

Compatibility of Neem (Azadirachta indica) and Beauveria bassiana for Control of Spodoptera exigua and the Theoretical Impact to the Agroecosystem Kurnianto, Agung Sih; Prastiwi, Santi; Dewi, Nilasari; Tanzil, Ahmad Ilham; Muhlison, Wildan; Wagiyana, Wagiyana
Biotropika: Journal of Tropical Biology Vol. 10 No. 2 (2022)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.02.01

Abstract

Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae) is the biggest threat to onion farming, especially in the vegetative phase. In severe attacks, this pest can cause yield losses of up to 100%. Compatibility is one way to increase the efficiency and effectiveness of pest control by using plant-based pesticides or biological agents. Neem Leaf Extract (Azadirachta indica, acronym=NLE) has an azadirachtin compound that can inhibit insect growth, reduce appetite, reproduction, and hatch eggs. The fungus Beauveria bassiana (acronym=Bb) can secrete chitinase, lipase, proteinase enzymes that are able to decompose insect cuticles. NLE was obtained through the extraction method of plant-based pesticides and stored at 4oC until the experiment time. The Bb used was a commercial B. bassiana inoculum in the flour form with a density of 4.5 x 1010 spores/gram (trade name = Natural BVR). To determine the advantages of compatibility, this study is held by a single toxicity test of Bb and NLE, and the combined toxicity test of both. This study used a completely randomized design (CRD) consisting of 6 treatments with 4 replications, each replication used 12 larvae (a total of 48 larvae). Combination toxicity has a higher toxicity than the single use of NLE and Bb. At the LC 95 level, the combination treatment of Bb and NLE was 1.06-1.15 times more toxic than the single treatment of Bb, and 6.87-7.79 times more toxic than the single treatment of NLE. NLE and Bb are considered to have high compatibility (strong synergistic with GI value <0.5). Theoretically, the compatibility of NLE plant-based pesticides and Bb is very promising to replace chemical pesticides that have long-term adverse effects on agroecosystems.
Co-Authors Agung S. Kurnianto Agung Sih Kurnianto Ahmad Ilham Tanzil Ahmad Muammar Kadafi Aini, Falzah Riski Khoirotul Aini, Wilda Qurrotul Ali Badrudin Arif Mohammad Siddiq Arizona, Salsa Dinta Arum, Ayu Puspita AYU LESTARI Banun Kusumawardani Berliand, Ghalih Daffa Bila, Zuyyina Hafsah Salsa Cahyono, Haris Dyah Ayu Savitri Efendi, Galang Prasetyo Febrianti, Wanda Hamidah Zakiyah Firdaus, Anggun Sausan Hanafi, Ahmad Rifqi A. Hanafi, Ahmad Rifqi Alief HARI PURNOMO Hari Sulistyowati Hartati, Kurnia Hasanah, Ela Apriliyanti Hidayat, Rachma Wahyu Hidayat, Yunus Hilda Ramadhani Ihtizar, Azizul Nur Irza Indra Nurtjahjaningtyas Indraswari, Nadya Oktarinz Kamalin, Indah M. Khoiruttamam, Muhammad Zulfa Khowatini, Husnul Laili, Fadlul Laily, Fajar Kurnia Lathifa, Nurul Lestari , Auralia Sakinah Lestari, Auralia Sakinah Magvira , Nur Laila Magvira, Nur Laila Margaretha, Novia Meliala, Susan Barbara Patricia Sembiring Mubarokah, Aiqe Seprinanda Muhammad Firdaus Muhlison , Wildan Mulyono, Abel Rahmad Nabil, Farrel Rayhan Nafsi, Ach. Sulthon Azizun Nanang Tri Haryadi Nindha Ayu Berlianti Nisak, Fauziatun Noviardhana, Astiningtia Nur Widodo Nurheni Wijayanto Oktaviani, Nabiila Salsabil Pamungkas, A. S. P. Pamungkas, Ardhi Satrio Paramita, Sofia Dwi Prameswari, Sekar Faradita Prastiwi, Santi Puspitasari, Dea Ayu Rachmawati, Rizki Amelia Ramadianto, R. Helmy Ratnasari, Tri Romdhon, Mochammad Syihab Rose Novita Sari Handoko Rosidah, Erin Indriana Sani, Amalia Putri Santosa, Enjel Meylita Sari, Sasmita Septiadi, Luhur Setiyono Setiyono Setyari, Agustina Dewi Shinta, Salsabila Nur Slameto Slameto Solekhah, Jihan Lutviatus Soleudin Efendi Sucipto , Irwanto Sulistiyowati, Hari Tecky Indriana Ummi Sholikhah Wagiyana Wagiyana, Wagiyana Wardani, Aditya Widianingrum, Desy Cahya Wildan Muhlison, Wildan Witjakso, Michele Aprilia Yulianto, Roni